• Natural Product Sciences
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• 제11권4호
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• pp.240-247
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• 2005

Phytochemical investigation of the berries of Juniperus phoenicea led to the isolation of 4 compounds, namely; scutellarin, isoscutellarin, shikimic acid and the new palmitoyl lactone derivative 16-hydroxy palmitic-1,16-olide. This is the first report for the occurrence of these compounds in the species grown in Libya. The identification of the isolated compounds was based on the application of different spectroscopic techniques. In addition, the antihepatotoxic effect of the aqueous decoction of the berries was investigated.

• Natural Product Sciences
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• 제20권3호
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• pp.160-169
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• 2014

44 compounds and 9 minerals were isolated from and detected in the New Zealand deer velvet antler Cervus elaphus var. scoticus L$\ddot{o}$nnberg. The chemical structures of (1 - 26) were identified on the basis of the spectroscopic methods and comparisons with literature, respectively. The structures were identified as cholesterol (CS, 6), 7-keto-CS (7), $7{\beta}$-hydroxy-CS (8), and $7{\alpha}$-hydroxy-CS (9), and included 12 steroid $3{\beta}$-O-(palmitic/stearic/myristic acid esters; PM/SA/MS) [CS-$3{\beta}$-O-PM (1 - 1), CS-$3{\beta}$-O-SA (1 - 2), CS-$3{\beta}$-O-MR (1 - 3), 7-keto-CS-$3{\beta}$-O-PM (2 - 1), 7-keto-CS-$3{\beta}$-O-SA (2 - 2), 7-keto-CS-$3{\beta}$-O-MR (2 - 3), $7{\beta}$-hydroxy-CS-$3{\beta}$-O-SA (3 -1), $7{\beta}$-hydroxy-CS-$3{\beta}$-O-PM (3 - 2), $7{\beta}$-hydroxy-CS-$3{\beta}$-O-MR (3 - 3), $7{\alpha}$-hydroxy-CS-$3{\beta}$-O-SA (4 - 1), $7{\alpha}$-hydroxy-CS-$3{\beta}$-O-PM (4 - 2), and $7{\alpha}$-hydroxy-CS-$3{\beta}$-O-MR (4 - 3)], dinonyl phthalate (5), 8 nucleic acids analogues [uracil (10), deoxyguanosine (11), deoxyuridine (12), uridine (13), deoxyadenosine (14), adenosine (15), inosine (16), and guanosine (17)], and the 9 free amino acids [L-phenylalanine (18), L-isoleucine (19), L-leucine (20), L-tyrosine (21), L-valine (22), L-proline (23), L-threonine (24), L-alanine (25), and L-hydroxyproline (26)]. Also, there are 8 kinds of amino acids [asparagine, serine, glutamine, glycine, histidine, arginine, methionine, and lysine], 2 sialic acids [N-acetylneuraminic acid (27), ketodeoxynonulosonic acid (28)], and 9 minerals [Na > K > Ca > Mg > Fe > Zn > B > Al > Cu] were detected from the autoaminoacid analyzer and ICP spectrometer, HPAEC-PAD/HPLC-FLD, respectively. 9 kinds of oxycholesterol-$3{\beta}$-O-fatty acid ester (2 - 1, 2 - 2, 2 - 3, 3 - 1, 3 - 2, 3 - 3, 4 - 1, 4 - 2, and 4 - 3) and 3 nucleic acids (12, 14, and 15) were isolated from the velvet antler for the first time. 6 kinds of steroids (7, 8, 9, 2 - 1, 3 - 1, and 4 - 1) were examined for their anti-proliferative effects against L1210, P388D1, K562, MEG-01, KG-1, MOLT-4, A549, HepG2, MCF-7, SK-OV-3, and SW-620 cancer cell lines. They showed anti-proliferative effects with $IC_{50}$ values of 0.06, 2.16, 2.42, > 50.0, 1.66 and $8.31{\mu}M$ against L1210, while the values were 24.05, 9.44, 5.22, 0.25. 9.48 and $49.77{\mu}M$ against P388D1, respectively. The others were inactive.

• 한국식품과학회지
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• 제25권6호
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• pp.794-800
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• 1993

알바비스커스 잎으로부터 세포외 지질을 대량 생산하는 효모를 분리하고 동정한 결과 Rhodotorula graminis SW 214로 확인하였으며, $25^{\circ}C$에서 8일간 진탕배양시 Rhodotorula graminis SW 214 세포의 지질 생산량은 7.31g/l였다. 배지내의 질소원과 탄소원의 농도는 세포의 지질 생산에 큰 영향을 주었는데 배지내의 질소원이 효모 성장에 거의 이용되고 탄소원이 아직 남아 있을때 지질 생산이 활발히 이루어졌다. 배지내에 질소원 농도가 높은 동안에는 지질 생산이 더디게 이루어졌다. Rhodotorula graminis SW 214 세포의 지질 생산량의 최적조건으로는 gluaminis SW 214 세포의 지질 생산량의 최적조건으로는 glucose 8%, yeast extract 2.5g/l, $KH_{2}PO_{4}\;1g/l,\;MgSO_{4}{\cdot}7H_{2}O\;0.2g/l$, pH6에서 8일간 배양할 때임을 알 수 있었고 효모건조 균체량은 8.05g/l, 지방 생산량은 8.89g/l였다. Rhodotorula graminis SW 214 세포의 지질의 TLC에 의한 분별 정량 결과는 triglyceride 75.16%, phospholipid 10.77%, free fatty acid 5.88, esterified sterol 4.94%, free sterol 3.25%였다. Rhodotorula graminis SW 214 세포의 지방의 주된 지방산 조성은 3-hydroxypentadecanate 37.90%, 3-hydroxypentadecanate 20.80%, trans-9-octadecanate 11.53%, cis-9-headecanate 10.93%, 15-methylhexadecanate 9.82%, 18-methylnonadecanate 4.63%, octadecanate 2.64%, 3-hydroxytridecanate 1.75%였다.