• 미생물학회지
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• 제49권2호
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• pp.165-171
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• 2013

순천대학교 지의류 은행(Lichen Bioresources Center)에서 571종의 지의류 내생 곰팡이를 분양받아 Candida albicans에 대한 항진균 활성을 조사하여 4종을 선정하였다. 선정된 4종의 지의류 내생 곰팡이의 배양액 내에 존재하는 항진균 활성을 알아보기 위해 disc diffusion method 방법을 이용한 결과 Stereocaulon sp. (1429,1430)가 C. albicans에 대한 항진균 활성이 가장 좋았으며, 다음으로는 Graphis sp. (1245), Cryptosporiopsis diversispora (0156) 순으로 활성이 좋게 나타났으며, 이들 지의류 내생곰팡이가 고체배지와 액체배지에서도 항진균 활성 물질을 생성하였다. 고체배지에서와 액체배지에서 공통적으로 활성이 있는 지의류 내생 곰팡이 배양액의 chloroform, ethyl acetate 용매 추출물의 C. albicans의 항진균 활성도를 측정한 결과 4종 모두 chloroform 추출물에서 높은 활성도를 나타냈다. Candida albicans, Candida glabrata, Candida tropicalis, Candida lusitaniae에 대한 항진균 활성은 Stereocaulon sp. (1429, 1430)의 chloroform 추출물에서 모두 98% 이상의 높은 저해율을 보였고 Stereocaulon sp. (1429) 배양액의 chloroform 추출액과 ethyl acetate 추출액을 GC/MS를 이용해 성분을 분석한 결과 hexadecanonic acid-methyl ester, 14-octadecenoic acid-methyl ester, octadecenoic acid-methyl ester가 새로운 항진균 물질일 가능성을 보여주었다.

• Journal of Acupuncture Research
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• 제28권2호
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• pp.89-95
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• 2011

Objectives : The purpose of this study was to investigate the composition of Rehmannia glutinosa's essential oils with Rehmanniae Radix herbal acupuncture Methods : I obtained the essential oils of Rehmannia Radix by hydrodistillation extraction method and supercritical fluid extraction(SFE) method, and then I analyzed those by GC/MS(gas chromatography/mass spectrum). Results : 1. With GC(gas chromatography) and GC/MS(gas chromatography/mass spectrum) analysis. I identified 9 compounds in the Rehmanniae Radix's essential oil obtained through the SFE method. The main compounds were as follows : Hexachloroethane(2.24%), N-Butyl-benzenesulfonamide(2.05%), hexadecanoic acid(1.93%), hexadecanoic acid, ethyl ester(3.49%), 9,12-Octadecadienoic acid(z,z)(2.70%), (9E)-9-Octadecenoic acid(6.14%), ethyl linoleate(4.43%), ethyl oleate(5.80%). 2. I failed to get Rehmanniae Radix's essential oil obtained through the hydrodistillation method. 3. With GC(gas chromatography) and GC/MS(gas chromatography/mass spectrum) analysis. I identified 4 compounds in the Rehmanniae Radix's essential oil obtained through the hydrodistillation method. The main compounds were as follows : Ethylbis(trimethylsilyl)amine(1.04%), 2-(Trimethylsiloxy)benzoic methyl ester(2.65%), Hexadecanoic acid trimethylsilyl ester(12.61%), octadecanoic acid, trimethylsilyl ester(6.28%). Conclusions : The substances by hydrodistillation method may not perfectly match with the substances by supercritical fluid extraction(SFE) method in essential oils extracted form Rehmanniae Radix. But, the main substances was assumed Hexadecanoic acid and octadecanoic acid.