• Journal of Life Science
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• v.6 no.4
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• pp.304-312
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• 1996

In the present report, a p[ossibility of the interaction fo Ser-33 and Asp-112 residues in folding of tryptophan synthase $\alpha$ subunit was explored by examining the effect of single or double substitution of these residues on folding of $\alpha$ subunit in E. coli. $\alpha$ subunit of which Ser-33 was substituted with Leu (SL33) was accumulated as insoluble aggregate form, when overproduced in E. coli, whereas $\alpha$ subunit of which Asp-112 was replaced by Asn (DN112) or Gly (DG112) was accumulated as soluble form to the similar extent as wild type $\alpha$ subunit was. When these alterations were combined into one protein, the synergistic effect of residues 33 and 112 on the amount of aggregate form was shown. The amount of doubly altered SL33/DG112 $\alpha$ subunit as aggregate form was increased 5-13 fold that of SL33 $\alpha$ subunit, and the amount of SL33/DG112 $\alpha$ subunit as aggregate form was decreased 3-4 fold that of SL33 $\alpha$ subunit. Aggregates are derived from the specific association of partially folded or unassembled subunits in the folding process. Therefore, this result suggests that residues 33 and 112 of $\alpha$ subunit may unteract during the folding of this enzyme in E. coli.

• Korean Journal of Weed Science
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• v.9 no.3
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• pp.221-229
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• 1989

This study is conducted to evaluate the effect of KIM-112 (Ca-3, 5-diode-4-propyonly-cyclohexane-1-carboxylate) application at 13, 8 and 3 days before heading for the possibility of lodging prevention, growing pattern of several organisms, growth and yield in paddy rice. KIM-112 was the most effective in reducing elongation of the third internode when it was applied at 13 days before heading(13DBH) and the second internode when it was applied at 8DBH and 3. KIM-112 gradually inhibited internode elongation of rice with increase in dosage starting from 1.0ga.i./10a. The culm length shortened by 7-17% at 3DBH. The maximum distribution of leaf blade at canopy structure was positioned higher on KIM-112 application than on untreated. Lodging index decreased by 8-47% on KIM-112 application and field lodging degree(0-5) was 5 at untreated but KIM-112 application was only 1.3-2.0 of 3 DBH and was little lodging of 8-13 DBH. Ripened grain ratio of KIM-112 application were higher than those of untreated. Yield increased by 27-37% at KIM-112 application.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2015.05a
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• pp.244-247
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• 2015

People who live in the Republic of Korea the world's leading information technology, all use a smartphone. When all the people calling 112 to report an emergency many use smartphone. Accordingly, this paper is not to be put on the emergency police report arguing for a second location backtrack through the 112 complainant to report a smartphone and not wasted also Identifying a false declaration to become beneficial to all citizens.

• Korean Journal of Veterinary Service
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• v.28 no.4
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• pp.393-405
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• 2005

In prion pathogenesis, the structural conversion of the cellular prion protein $(PrP^c)$ to its abnormal isomer $(PrP^{Sc})$ is believed to be a major event. The susceptibility or resistance to natural sheep scrapie is associated with polymorphisms of host PrP gene (PRNP) at amino acid residues 136, to a lesser extent 154. The 112 residue in ovine PrP displays a natural polymorphism, Methionine to Threonine, which has not been thoroughly investigated. However the cell-free conversion assay showed that ARQ with Thr112 $(T_{112}ARQ)^{1)}$ presents lower convertibility to $PrP^{Sc}$than wild type ARQ $(M_{112}ARQ)$ [1] In this study we generated ovine recombinant PrPs of 112 allelic variants by metal chelate affinity chromatography and cation exchange chromatography. The final purity of the ovine PrP ARQ was more than $95\%$. These variants showed similar immunoreactivity against anti-PrP monoclonal antibodies in Western blot and ELISA. The refolded $M_{112}ARQ$ and $M_{112}ARQ$ presented the secondary structural content to similar extent via CD spectroscopy analysis. The inherited structural features of $M_{112}ARQ$ and $M_{112}ARQ$ under the different biophysical conditions are in the middle of investigation.

• Proceedings of the Korean Society for Technology of Plasticity Conference
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• 1995.10a
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• pp.167-171
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• 1995

The formation of preferred orientations in cold rolled and recrystallized Inconel 690 sheets was studied by the x-ray texture measurements and TEM observations. The increasing{220} pole intensity in the plane normal at the higher reductions was related to the{110}<112> texture component. The rolling texture of the Inconel 690 was the pure metal type. THe dislocation cells were found in the near{110}<112> oriented grains. The onset of deformation twins in the {112}<111>oriented grains. The onset of deformation twins in the {112}<111> oriented grains. The onset of deformation twins in the {112}<111> oriented grains caused the weakening of {112}<111> and the development of {552}<115> in the rolling texture. The annealing texture of the Inconel 690 sheets was dependent on the annealing temperature. The annealing texture of 750$^{\circ}C$ annealed sheets was similar to the cold rolling texture. The major preferred orientations of the 950$^{\circ}C$ annealed specimens were {112}<110> and {001}<110>. The formation of fine and closely spaced annealing twins in the specimen annealed at 1150$^{\circ}C$ led to the randomization of the annealing texture.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.43 no.2
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• pp.71-76
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• 1998

To define the relations between endogenous GA levels and growth and flowering in short-day plant sorghum, growth retardant BX-112 was applied to two sorghum genotypes, wild-type and phytochrome B mutant (phyB-1), which grows faster and flowers earlier than the wild-type. BX-112 and $GA_3$ were applied as a soil drench, and plant height, culm length, and date to floral initiation were investigated. Endogenous GAs contents were measured with GC-MS-SIM. BX-112 treatments inhibited shoot growth in both genotypes and drastically reduced $GA_1$ and $GA_8$ levels. With increasing BX-112 concentrations, $GA_1$ concentrations declined linearly, but caused the accumulation of intermediates from $GA_12$ to $GA_20$. This result implies that $GA_1$ is the major active endogenous GA in shoot elongation in a short day plant sorghum. The inhibition of plant growth in both of wild type and phyB-1 by BX-112 was very similar, while BX-112 effects on floral initiation in two types of plants differed significantly. Floral initiation of phyB-1 was not affected by BX-1l2, but that of wild-type was delayed as BX-1l2 concentration increased. Because BX-112 treatment causes accumulation of biosynthetic intermediates between synthetic pathway from $GA_12$ to $GA_20$ and because phyB-1 is altered in GA metabolism in this same region of the early C13-hydroxylation pathway, BX-112 may fail to block flowering of phyB-1.

• The Korean Journal of Mycology
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• v.44 no.3
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• pp.176-183
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• 2016

Bacillus velezensis CC112 inhibited the mycelial growth of several plant pathogens, including Rhizoctonia solani, causing damping-off on ginseng. The control efficacies of B. velezensis CC112 against R. solani by seed dipping in LB and BSM broth diluted 10 times, soil dipping, and soil drenching with LB broth diluted 10 times were 65.8%, 67.1%, and 64.2%, respectively. Treatment of soil drenching with the 100 times diluted prototype of B. velezensis CC112 against R. solani and Pythium sp. by soil revealed control efficacies of 77.3% and 65.7%, respectively. These results indicate that B. velezensis CC112 is a prospective biofungicide for the biological control of ginseng damping off.

• Transactions of Materials Processing
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• v.3 no.4
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• pp.392-400
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• 1994

The formation of preferred orientations in the cold rolling texture of the Inconel 690 sheets was studied by the x-ray texture measurements and TEM observations. The increasing {220} pole intensity in the plane normal at the higher reductions was related to the {110}<112> texture component. The rolling texture of the Inconel 690 was the pure metal type which could be described by {112}<111>, {123}<634> and {110}<112> orientations. The dislocation cells were found in the near {110}<112> oriented grains. The onset of deformation twins in the {112}<111> oriented grains caused the weakening of {112}<111> and the development of {552}<115> in the rolling texture.

• Journal of the Optical Society of Korea
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• v.19 no.1
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• pp.112-112
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• 2015

• Bulletin of the Korean Chemical Society
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• v.10 no.1
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• pp.112-112
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• 1989