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METACESTODES INFECTION IN BLACK BENGAL GOATS IN BANGLADESH

  • Islam, M.K.;Mondal, M.M.H.;Das, P.M.
    • Asian-Australasian Journal of Animal Sciences
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    • 제8권1호
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    • pp.13-16
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    • 1995
  • In this study out of 3036 Black Bengal goats examined, 1755(57.80%) were infected with different types of metacestodes. The metacestodes were Cysticercus tenuicollis(54.54%), Coenurus cerebralis(6.32%) and hydatid cysts(11.13%). Animals > 18 months old were relatively more susceptible to Cysticercus tenuicollis and Coenurus cerebralis infection. On the contrary, animals > 8 months to 18 months old were more susceptible to hydatid infection, Both male and female goats were found to be equally infected with the metacestodes. A fully grown Coenurus cerebralis was also recovered from the abdominal muscles of a goat. In Cysticercus tenuicollis infection, pathological effects were not so marked. However, in few cases of Coenurus cerebralis infection the bones of the skull were found to become thin and soft. The hydatid cysts were found to develop in the vital organs by replacing the tissues, thus impairing normal functions.

개의 발정개시 후 초음파 진단에 의한 수정적기 판단기술 개발

  • 이장희;지달영;박성재;최선호;류일선;김상운;정경용;백순화;김창근
    • 한국동물번식학회:학술대회논문집
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    • 한국동물번식학회 2003년도 학술발표대회 발표논문초록집
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    • pp.87-87
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    • 2003
  • 본 연구는 개의 수태율 향상을 위한 인공 수정적기를 구명하기 위하여 발정개시 후 초음파를 이용하여 난소내 난포발달 상태를 진단하였다. 자연적으로 발정이 개시된 Greyhound 2두에 대해서 초음파기(SA 600S, 메디슨, 한국)와 3.SMHz probe를 이용하여 난포발달에 대한 초음파화상을 얻었다. 외음부가 종창하고 출혈이 확인된 첫날을 발정개시일(Day 1)로 하였으며 발정개시 후 11일째, 13일째 및 15일째 난포발달 상태를 조사한 결과 좌우 난소에 각각 3개 및 4개의 난포가 발달중에 있었으며, 난포의 크기는 좌우 각각 11일째에 6, 9, 17mm(좌측)와 6, 9, 6, 5mm(우측)였으며, 13일째에는 각각 10, 11, 11mm 및 9, 14, 13, 12mm였다. 15일째에는 이미 배란이 일어나서 난포상을 관찰할 수 없었으나 배란흔적은 관찰할 수 있었다. 본 실험에서는 발정개시 후 11, 13, 15일째 0.25$m\ell$ 주입기를 이용하여 그레이하운드 동결정액으로 인공수정하였으며, 이때 동결정액(좋은유전자 Co.)의 융해 후 활력 있는 정자수는 3$\times$$10^{7}$ spermatozoa/dose(0.25$m\ell$)였으며, 자궁경관내 주입기선단의 관통 여부를 촉진한 후 자궁내 정액 주입을 확인할 수 있었다. 난포발달에 대한 초음파 진단결과 Greyhound에 있어서 1회 인공수정시에는 발정개시 후 15일째가 높은 수태를 위한 수정적기라고 사료되었으며 이러한 결과는 Concannon 등(1989)이 배란 후 2일째가 수정적기로 판단한 연구 결과와 유사한 경향을 나타내었다.

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Qualitative and Quantitative Analysis of Thirteen Marker Components in Traditional Korean Formula, Samryeongbaekchul-san using an Ultra-Performance Liquid Chromatography Equipped with Electrospray Ionization Tandem Mass Spectrometry

  • Seo, Chang-Seob;Shin, Hyeun-Kyoo
    • Natural Product Sciences
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    • 제22권2호
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    • pp.93-101
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    • 2016
  • For efficient quality control of the Samryeongbaekchul-san decoction, a powerful and accurate an ultra-performance liquid chromatography (UPLC) coupled with electrospray ionization (ESI) tandem mass spectrometry (MS) method was developed for quantitative analysis of the thirteen constituents: allantoin (1), spinosin (2), liquiritin (3), ginsenoside Rg1 (4), liquiritigenin (5), platycodin D2 (6), platycodin D (7), ginsenoside Rb1 (8), glycyrrhizin (9), 6-gingerol (10), atractylenolide III (11), atractylenolide II (12), and atractylenolide I (13). Separation of the compounds 1 - 13 was performed on a UPLC BEH $C_{18}$ column ($2.1{\times}100mm$, $1.7{\mu}m$) at a column temperature of $40^{\circ}C$ with a gradient solvent system of 0.1% (v/v) formic acid aqueous-acetonitrile. The flow rate and injection volume were 0.3 mL/min and $2.0{\mu}L$. Calibration curves of all compounds were showed good linearity with values of the correlation coefficient ${\geq}0.9920$ within the test ranges. The values of limits of detection and quantification for all analytes were 0.04 - 4.53 ng/mL and 0.13 - 13.60 ng/mL. The result of an experiment, compounds 2, 6, 12, and 13 were not detected while compounds 1, 3 - 5, and 7 - 11 were detected with 1,570.42, 5,239.85, 299.35, 318.88, 562.27, 340.87, 12,253.69, 73.80, and $115.01{\mu}g/g$, respectively.

AKARI IRC SURVEY OF THE LARGE MAGELLANIC CLOUD: AN OVERVIEW OF THE SURVEY AND A BRIEF DESCRIPTION OF THE POINT SOURCE CATALOG

  • Ita, Yoshifusa;Kato, Daisuke;Onaka, Takashi;AKAR.LMC survey team
    • 천문학논총
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    • 제27권4호
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    • pp.165-169
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    • 2012
  • We observed an area of 10 $deg^2$ of the Large Magellanic Cloud using the Infrared Camera (IRC) onboard AKARI. The observations were carried out using five imaging filters (3, 7, 11, 15, and $24{\mu}m$) and the prism disperser ($2-5{\mu}m$, ${\lambda}/{\Delta}{\lambda}{\sim}20$) equipped in the IRC. This paper presents an outline of the survey project and also describes very briefly the newly compiled near- to mid-infrared point source catalog. The $10{\sigma}$ limiting magnitudes are 17.9, 13.8, 12.4, 9.9, and 8.6 mag at 3.2, 7, 11, 15 and $24{\mu}m$, respectively. The photometric accuracy is estimated to be about 0.1 mag at $3.2{\mu}m$ and 0.06 - 0.07 mag in the other bands. The position accuracy is 0.3" at 3.2, 7 and $11{\mu}m$ and 1.0" at 15 and $24{\mu}m$. The sensitivities at 3.2, 7, and $24{\mu}m$ are roughly comparable to those of the Spitzer SAGE LMC point source catalog, while the AKARI catalog provides the data at 11 and $15{\mu}m$, covering the near- to mid-infrared spectral range continuously.

Characterization of CYP125A13, the First Steroid C-27 Monooxygenase from Streptomyces peucetius ATCC27952

  • Rimal, Hemraj;Subedi, Pradeep;Kim, Ki -Hwa;Park, Hyun;Lee, Jun Hyuck;Oh, Tae-Jin
    • Journal of Microbiology and Biotechnology
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    • 제30권11호
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    • pp.1750-1759
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    • 2020
  • The characterization of cytochrome P450 CYP125A13 from Streptomyces peucetius was conducted using cholesterol as the sole substrate. The in vitro enzymatic assay utilizing putidaredoxin and putidaredoxin reductase from Pseudomonas putida revealed that CYP125A13 bound cholesterol and hydroxylated it. The calculated KD value, catalytic conversion rates, and Km value were 56.92 ± 11.28 μM, 1.95 nmol min-1 nmol-1, and 11.3 ± 2.8 μM, respectively. Gas chromatography-mass spectrometry (GC-MS) analysis showed that carbon 27 of the cholesterol side-chain was hydroxylated, characterizing CYP125A13 as steroid C27-hydroxylase. The homology modeling and docking results also revealed the binding of cholesterol to the active site, facilitated by the hydrophobic amino acids and position of the C27-methyl group near heme. This orientation was favorable for the hydroxylation of the C27-methyl group, supporting the in vitro analysis. This was the first reported case of the hydroxylation of cholesterol at the C-27 position by Streptomyces P450. This study also established the catalytic function of CYP125A13 and provides a solid basis for further studies related to the catabolic potential of Streptomyces species.

지역사회 거주 심장질환 환자들을 위한 모바일 건강중재: 통합적 문헌고찰 (Mobile Health Interventions for Community-Dwelling Patients with Heart Diseases: An Integrative Review)

  • 고지운;강현욱
    • 중환자간호학회지
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    • 제13권1호
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    • pp.63-75
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    • 2020
  • Purpose : The purpose of this study was to review mobile health (mHealth) interventions based on studies from online databases for community-dwelling patients with heart diseases. Methods : Six databases (RISS, KISS, PubMed, CINAHL, EMBASE, and SCOPUS) were searched to select studies conducted from January 1 to September 30, 2010. After this, quality appraisals were carried out using the Scottish Intercollegiate Guidelines Network checklist and a total of 11 studies were selected. Results : The selected 11 studies included 7 randomized controlled studies, 1 quasi-experimental study and 3 pilot studies. The main components of mHealth interventions included symptom monitoring at home, provisions for individualized messages for health management using text messaging, telephone or smart phone applications, and running websites for symptom monitoring or health education. Intervention periods varied from 6 weeks to 12 months. The findings of the studies suggested that the mHealth interventions were effective in improving self-management of heart diseases, quality of life, and decreasing symptoms. Conclusions : The results of the review suggested that mHealth interventions had positive effects on community-dwelling patients with heart diseases. More mHealth intervention studies need to be conducted in Korea to aid community-dwelling patients with heart diseases.

집진용 세라믹 필터의 제조 및 공기 투과 특성 (Preparation of Ceramic Foam Filter and Air Permeability)

  • 박재구
    • 한국대기환경학회지
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    • 제16권4호
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    • pp.381-388
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    • 2000
  • Ceramic foam prepared with cordierite as a starting material by foam method was tested to evaluate the feasibility as a filter for the dust collection in hot gas. Two different types of agents Benzethonium chloride (BZTC, C27H42NO2Cl) and Sodium Lauryl Sulfate(SLS, CH3(CH2)11OSO3Na) were used as foaming agents in foaming process. Porosityof ceramic foam was about 80% and mean pore size were 100${\mu}{\textrm}{m}$ for SLS agent and 200 ${\mu}{\textrm}{m}$ for BZTC. It was observed that ceramic foam was composed of continuous macro-pore structure with opening windows interconnecting macro-pores. The surface of ceramic foam support of was coated with cordierite particles ranged from 20${\mu}{\textrm}{m}$ to 50${\mu}{\textrm}{m}$ Meso-pore size in the coating layer on ceramic foam was below 10${\mu}{\textrm}{m}$. While air permeability of the support increased with increasing macro-pore size coated ceramic filters showed a constant permeability without regard to the macro-pore size of the support. The permeabuilities of support varied in the range of 600$\times$10-13m2 to 1000$\times$10-13m2. For the case of coated ceramic filter it was about 200$\times$10-13m2. As a result of particle trapping test by using fly ash the particle removal efficiency was over the 99.9%.

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A report of 7 unrecorded bacterial species isolated from several Jeju soil samples in 2016

  • Kim, Ju-Young;Jang, Jun Hwee;Maeng, Soohyun;Kang, Myung-Suk;Kim, Myung Kyum
    • Journal of Species Research
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    • 제7권2호
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    • pp.151-160
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    • 2018
  • Seven bacterial strains, 15J4M-1, 15J13-8, 16MFM10, 15J1-8, SR1-5-4, 15J13-6, and 15J8-11 assigned to the phylum Actinobacteria, Bacteroidetes, and Firmicutes were isolated from soil samples collected from Jeju, Korea. Phylogenetic analysis based on 16S rRNA gene sequence revealed that strains 15J4M-1, 15J13-8, 16MFM10, 15J1-8, SR1-5-4, 15J13-6, and 15J8-11 were most closely related to Bacillus selenatarsenatis $SF-1^T$ (with 99.4% similarity), Brevibacterium luteolum $CF87^T$ (99.5%), Carnobacterium iners CCUG $62000^T$ (99.6%), Exiguobacterium profundum $10C^T$ (99.3%), Larkinella insperata LMG $22510^T$ (99.3%), Pseudokineococcus lusitanus CECT $7306^T$ (99.4%), and Spirosoma endophyticum $EX36^T$ (99.3%), respectively. This is the first report of these seven species in Korea.