• Korean Chemical Engineering Research
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• v.58 no.1
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• pp.113-121
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• 2020

In batch experiments, the adsorption of 7-epi-10-deacetylpaclitaxel was studied using Sylopute. Experimental equilibrium data were applied to Langmuir, Freundlich, Temkin and Dubinin-Radushkevich isotherm models. Among the four isotherm models tested, the Langmuir isotherm model gave the highest accuracy. The adsorption capacity was found to decrease with increases in temperature and the adsorption of 7-epi-10-deacetylpaclitaxel onto Sylopute was a favorable physical process. Adsorption kinetic data agreed very well with the pseudo-second-order kinetic model, while boundary layer diffusion and intraparticle diffusion did not play a key role in the adsorption process. The process of 7-epi-10-deacetylpaclitaxel adsorption onto Sylopute was exothermic and nonspontaneous. Also, the adsorption isosteric heat was independent of surface loading indicating an energetically homogeneous adsorbent.

• Korean Chemical Engineering Research
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• v.54 no.1
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• pp.89-93
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• 2016

In this study, we investigated the separation behavior of the anticancer agent paclitaxel and its semi-synthetic precursor 10-deacetylpaclitaxel (10-DAP) from plant cell cultures. As a result of sequential separation/purification performed by biomass extraction with solvent, liquid-liquid extraction, adsorbent treatment, hexane precipitation, and fractional precipitation, the adsorbent treatment was found to be the most effective in separating and recovering 10-DAP from paclitaxel. The optimal adsorbent type, crude extract/adsorbent ratio, and adsorbent treatment temperature were sylopute, 1:1.5 (w/w), and $20^{\circ}C$, respectively. The separation/recovery of 10-DAP from paclitaxel was 74.1% in adsorbent treatment process under optimal conditions.