• The Journal of Korean Society for School & Community Health Education
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• v.20 no.1
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• pp.57-68
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• 2019

Objectives: We would like to manage university student's health factors by researching the connection between BMI and oral health and behavior of university students. And provide basic data by integrating for weight management programs that target overweight people can improve oral health. Methods: Self-evaluation questionnaires were surveyed for 315 students who agreed to participate in the survey, using questionnaires used in the preceding study were modified and complemented. We analyzed the data with frequency analysis, descriptive statistics, t-test, chi-square test와 ANOVA, Spearman correlation coefficient by using IBM SPSS Statistics 21.0 (IBM Co., Armonk, NY, USA). The significance level for significance was set at 0.05. Results: People who smoke and alcohol drink have a lower score in their oral health behavior and oral health knowledge than those who do not. There was a significant difference between the BMI group, in the oral health behavior and oral health knowledge scores. BMI and smoking (r=0.230, p<0.001), alcohol drinking (r=0.121, p<0.05) were significant positive correlation. BMI and sleep time (r=-0.127, p<0.05), oral health behaviors (r=-0.133, p<0.001) were significant negative correlation. oral health behaviors and oral health knowledge (r=0.344, p<0.001) were significant positive correlation. Conclusion: Schools and communities will need to be educated about smoking and drinking, while at the same time developing programs that can improve oral health by integrating weight management programs.

• The Korean Journal of Mycology
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• v.23 no.2 s.73
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• pp.129-138
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• 1995

DNA fragment being able to restore in vitro activity of ${\beta}-1,3-glucan$ synthase was cloned by transformation of the Saccharomyces cerevisiae LP353 mutant strain with genomic library constructed in the YCp50. For the selection of transformants which showed no detectable phenotype linked to recovery of the defect in ${\beta}-1,3-glucan$ synthase activity, the colony autoradiography was succesfully applied. The restriction map of the cloned DNA fragment, which is 8.5-kb in length, was constructed. Both the YEplac195 and the YCp50 carrying the 8.5-kb fragment increased ${\beta}-1,3-glucan$ synthase activity of LP353 by two fold. Neither the YEplac195 nor the YCp50 carrying the 8.5-kb DNA fragment, however, complemented the temperature-dependent osmotic sensitivity which is another distinctive phenotype of LP353. Subcloning experiments indicated that a functional region was located in 4.8-kb BglII-KpnI fragment. The 4.8-kb fragment was also able to increase the level of ${\beta}-1,3-glucan$ content in cell wall as well as the resistance of cells to cell wall lytic enzyme, ${\beta}-1,3-glucanase$. The growth rate of the LP353 with 4.8-kb fragment was almost same as that of wild type strain in liquid medium with 1.2 M sorbitol at nonpermissive temperature. Taken these results together, the 4.8-kb fragment seemed to contain the BGS2 gene for ${\beta}-1,3-glucan$ synthase activity in yeast S. cerevisiae.

• Microbiology and Biotechnology Letters
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• v.17 no.1
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• pp.1-7
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• 1989

To enhance the capability of starch fermentation of the transformant TSD-14, the heat treated protoplasts of TSD-14 were fused with the protoplasts of C. tropicalis (lys$^-$) in the presence of 30% (w/ v) PEG and 20 mM CaC1$_2$. Fusants were selected by nutritional complementation on minium medium and the fusion frequency was 4.4$\times$10$^{-5}$. All fusants tested were possessed of complemented traits concerning carbon compound assimilation, and the cell volumes of the fusants were approximately 1.5 times larger than the parental strains. The fusants were genetically very stable, and were able to hydrolyze alpha 1,4-glucosidic linkage as well as alpha 1,6-linkage of starch contrary to one of parents TSD-14, The most promising fusant FSC-14-75 produced 8.7% (v/v) of ethanol from 15% liquefied potato starch medium, but the result was enhanced to 9.3% (v/v) by addition of 0.3% peptone. The corresponding fermentation efficiency was 86.0%.

• Journal of Korean Academy of Nursing
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• v.30 no.1
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• pp.18-28
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• 2000

The Purpose of this study is to extend the understanding and knowledge of menopause by comparing menopausal symptoms of married women and nuns in order to improve health and the quality of life for women. In this study a questionnaires were distributed to 116 nuns and 121 married women, from forty to fifty nine years old in Daegu metropolitan city. This study used the measurement of menopausal symptoms (Cronbach's Alpha=0.96) corrected and complemented by this student with consideration of various literature centered around menopausal symptoms, suggested by Janette M. Perz as 51 questions including 4 realms; [mental psychological factors], [physical physiological factors], [eyesight factors], and [urinary generative factors] in order to measure the degree of menopausal symptoms. The results are as follows : 1. There was a significant difference between educational background, religion, occupation, leisure time, satisfaction of marriage or ascetic life, existence of hormonal treatment, name of medical operation, and existence of counselling about menstruation as general and physiological characteristics of the nuns' group and the married women's group. 2. The menopausal symptoms of the nuns' group and the married women's group according to order in which they were presented were; 'feeling tired and lacking in energy(1.71 points)' 'vision not clear or clouded(1.69 points)', and 'be forgetful (1.57 points)' in nuns' group. 'be forgetful (1.87 points)', 'vision not clear or clouded(1.83 points)' and 'feeling tired and lacking in energy(1.76 points)' in the married women's group. The symptoms which showed the highest rank of menopausal symptoms had a maximum score of 4 points. 3. There was a statistical a significance (t=-3.9807, p<.0001) between the two groups which showed, on an average, 57.92 points in the married women's group and 43.03 points in the nuns' group from 0 to 196 of the possible points of menopausal symptoms. In difference of menopausal symptoms by menstrual aspect of the nuns' group and the married women's group, there was statistically significant difference between the two groups, showing 44.81±26.07 score in the nuns' group and 72.33±35.29 score in the married women's group as the mean score of the groups with no menstruation(t=-4.1132, p=0.0001). 4. The differences in menopausal symptoms with respect to the general and physiological characteristics of the nuns' group and the married women's group were that the nuns' group showed less menopausal symptoms on all the items than that of the married women's group. Finally, in these results, the married women's group showed higher menopausal symptoms than that of the nuns' group. Especially as the score of menopausal symptoms since the climacteric was very high it is confirmed to be a new phenomenon. Accordingly, it is considered to be necessary to carry out an indepth study of the factors related to establishing a strategy for nursing service.

• Journal of Life Science
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• v.16 no.7 s.80
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• pp.1112-1118
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• 2006

Bordetella bronchiseptica, the agent of swine atrophic rhinitis and kennel cough in dogs, is a mucosal pathogen and produces the hydroxamate type alcaligin siderophore under iron-limited conditions. Genes involved in alcaligin siderophore biosynthesis are contained in an alcABCDE operon. In order to provide direct evidence for the role of AlcA in alcaligin biosynthesis, we needed a B. bronchiseptica mutant carrying alcA gene deletion. A 0.6 kb alcA 5'-flanking and 0.7kb 3'-flanking DNA fragments were PCR amplified with the use of pCP1.11 as a template DNA. The 5'-and 3'-flanking DNA fragments were joined in a suicide plasmid, resulting in a recombinant suicide plasmid pDM1. After introduction of pDM1 into B. bronchiseptica by conjugation, the allelic exchange technique was performed and a B. bronchiseptica alcA deletion mutant, named B. bronchiseptica H1, was obtained. The mutant strain produced reduced amount of siderophore as expected. When a plasmid containing complete alcA gene was transformed back into the mutant, the complemented mutant recovered ability of siderophore production. These results indicated that AlcA is one of essential components for the alcaligin siderophore biosynthesis. The mutant strains obtained in this study will be used in the further studies for the biochemical function of AlcA.

• The Korean Journal of Emergency Medical Services
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• v.13 no.1
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• pp.115-127
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• 2009

Purpose : This study is descriptive correlation research aimed to examine knowledge and performance ability of cardiopulmonary resuscitation of student attending the college and provide basic materials in developing cardiopulmonary resuscitation program. Methods : Data collection was conducted with the 1st, 2nd and 3rd grade college students sampled randomly by giving them questionnaire from Sep. 1 to 4, 2008. It used the research instrument used by Choi Hyang Ok(2006) and revised and complemented by the researcher and 200 data excluding questionnaire showing unfaithful responses were used for analysis. Data collected were verified with technical statistics using SPSS/PC(version 12.0), t-test, ANOVA, and Pearson's correlation coefficient. Results : The results of this study are as follows. 1. Subjects' knowledge score of cardiopulmonary resuscitation was average .40(out of 1) and their performance ability score was average 2.39. 2. In the knowledge of cardiopulmonary resuscitation according to subjects' general characteristics, score was higher between over thirty and below twenty in age and in male subjects by sex and there were statistically significant differences(t=3.069, p=.029). 3. In the knowledge of cardiopulmonary resuscitation related to subjects' cardiopulmonary resuscitation, there were statistically significant differences in such variables as 'have you ever heard about cardiopulmonary resuscitation' (t=3.685, p=.000), 'presence or absence of education of cardiopulmonary resuscitation'(t=3.764, p=.000) and 'experience of observing cardiopulmonary resuscitation'(t=4.747, p=.000). In the performance ability of cardiopulmonary resuscitation by subjects, there were also statistically significant differences in such variables as 'have you ever heard about cardiopulmonary resuscitation' (t=2.269, p=.024), 'presence or absence of education of cardiopulmonary resuscitation' (t=3.541, p=.000) and 'experience of observing cardiopulmonary resuscitation' (t=8.118, p=,000). 4. Relation between the knowledge and the performance ability of cardiopulmonary resuscitation showed positive correlations as the more knowledge, the higher performance ability(r=.570, p=.000). Conclusion : Consequently, it was found that the knowledge and the performance ability of cardiopulmonary resuscitation by subjects were below the average and their levels of knowledge and performance ability were varied. Therefore, if 1 : 1 education with theory and practice considering knowledge and performance ability of each student in performing cardiopulmonary resuscitation education with college students is conducted, more lives can be saved by applying cardiopulmonary resuscitation not to be embarrassed when they encounter cardiac arrest case and contribute to the increase of survival of cardiac arrest patients.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.30 no.1
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• pp.46-54
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• 1997

Sporulation in the fission yeast Schizosaccharomyces pombe has been regarded as an important model of cellular development and differentiation. S. pombe cells proliferate by mitosis and binary fission on growth medium. Deprivation of nutrients especially nitrogen sources, causes the cessation of mitosis and initiates sexual reproduction by malting between two sexually compatible cell types. Meiosis is then followed in a diploid cell in the absence of nitrogen source. DNA fragment complemented with the mutations of sporulation gene was isolated from the S. pombe gene library constructed in the vector, pDB 248' and designated as pDB (spo 5)1. We futher analyzed six recombinant plasmids, pDB (spo 5)2, pDB(spo 5)3, pDB(spo 5)4, pDB(spo 5)5, pDB(spo 5)6, pDB(spo 5)7, and found each plasmids is able to rescue the spo 5-2, spo 5-3, spo 5-4, spo 5-5, spo 5-6, spo 5-7, mutations, respectively. Mapping of the integrated plasmid into the homologous site of the S. pombe chromosomes demonstrated that pDB (spo 5)1, and pDB (spo 5)R1 contained the spo 5 gene. Transcipts of spo 5 gene were analyzed by Northern hybridization. Two transcripts of 3.2 kb and 25 kb were detected with 5 kb Hind III fragment containing a part of the spo 5 gene as a probe. The small mRNA (2.5 kb) appeared only when a wild-type strain was cultured in the absence of nitrogen source in which condition the large mRNA (3.2 kb) was produced constitutively. Appearance of a 2.5 kb spo 5-mRNA depends upon the function of the mei1, mei2 and mei3 genes.

• Asian-Australasian Journal of Animal Sciences
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• v.15 no.5
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• pp.699-707
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• 2002

Diets with foliage of Flemingia (Flemingia macrophylla) or Jackfruit (Artocapus heterophyllus were fed to goats with the objective to study nitrogen (N) balance and effect of a daily supplementation of polyethylene glycol (PEG) on intake and digestion. In experiment 1, three male Alpine${\times}$Jamnapary goats with initial weights varying from 26.9 to 27.7 kg were used in a $3{\times}3$ Latin square design in the dry season. Three Alpine${\times}$Bachthao crosses, 15.3-16.7 kg, were used in the same design in the wet season. The three diets were based on chopped whole sugar cane complemented with the two green foliages, Jackfruit and Flemingia, or soybean meal (SBM). The level of dry matter (DM) offered was 4% of body weight (BW), 2.7% as foliage and 1.3% as chopped whole sugar cane. The amount of SBM offered was calculated to give the same amount of crude protein (CP) as the foliages. Each experimental period lasted 32 days (14 days for adaptation, 7 days for collection and 10 days for rest). Feed intake, apparent digestibility of DM, organic matter (OM), CP, neutral detergent fiber (NDF) and acid detergent fiber (ADF) and retained nitrogen (N) were measured by total faecal and urine collection. In experiment 2, four male goats (Alpine${\times}$Jamnapary) with initial weights from 17.1 to 23.1 kg were used in a $4{\times}4$ Latin square design. The four treatments were Jackfruit or Flemingia with or without addition of PEG, which was fed at a level of 5 g/goat and day by mixing with a small amount of rice bran. Each experimental period lasted 15 days (8 days for adaptation, 7 days for collection). Measurements were done as in experiment 1. The DM digestibility was highest (65.9-74.3%) for goats fed the SBM diet in both the dry and wet season. The DM digestibility of goats fed the Jackfruit and the Flemingia diets was similar in both the dry (58.6-59.2% respectively) and the wet season (53.9-56.1% respectively). The CP digestibility was highest (73.0-73.6%) for the SBM diet followed by the Jackfruit diet (47.0-38.5%) and was lowest (36.8-30.0%) for the Flemingia diet in both dry and wet seasons, respectively. The NDF digestibility was low for both the Jackfruit (36.4%) and Flemingia (38.0%) diets in the wet season. All diets resulted in a positive N balance. The N retention was highest (0.465-0.604 g/kg $W^{0.75}$) in the SBM diets and lowest (0.012-0.250 g/kg $W^{0.75}$) in the Flemingia diet. Addition of PEG had no effect on feed intake for any of the diets. PEG added in the Flemingia diet had a positive effect only on NDF digestibility, but the digestibility of the Jackfruit diet was significantly increased. Supplementation with PEG reduced digestibility and N retention of Flemingia, possibly because of the low tannin level, but increased digestibility and N retention for Jackfruit foliage.

• Journal of the Korean Applied Science and Technology
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• v.37 no.5
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• pp.1138-1150
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• 2020

In this study, an experiment was conducted to develop a sunscreen with antioxidant effects by simultaneously investigating the antioxidant and UV protection capabilities of various plant extracts. First, to investigate the UV-blocking ability of 33 kinds of plant extracts, the absorbance spectrum between the UV wavelength of 280 to 400 nm was investigated. Arrowroot, graviola, wheat sprout, sangbaek skin, thorn meal, lacquer, etc. 11 species were selected. The total polyphenol content, total flavonoid content, and DPPH radical scavenging activity of the selected plant extracts are measured to examine the degree of antioxidant activity, and from this, it is a plant extract that has excellent UV protection and antioxidant activity at the same time. The species was selected. A gel-shaped cream is prepared by mixing the selected gold, hops, and licorice extracts in a ratio of 1:1:1, and the UV protection effect of this cream is measured when the cultured cells are irradiated with UV rays. Determined by the method. As a result of the study, it was confirmed that the selected mixture of plant extracts complemented each other in terms of ultraviolet absorption ability and increased cell damage protection effect. Through these results, it was confirmed that it was possible to develop a sunscreen with an antioxidant effect if the antioxidant and sunscreen capabilities of various plant extracts were determined at the same time.

• Journal of Gifted/Talented Education
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• v.22 no.3
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• pp.619-637
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• 2012

The purpose of this study is to develop a new program for elementary math-gifted students by using 'Girih Tililng' and apply it to the elementary students to improve their math-ability. Girih Tililng is well known for 'the secrets of mathematics hidden in Mosque decoration' with lots of recent attention from the world. The process of this study is as follows; (1) Reference research has been done for various tiling theories and the theories have been utilized for making this study applicable. (2) The characteristic features of Mosque tiles and their basic structures have been analyzed. After logical examination of the patterns, their mathematic attributes have been found out. (3) After development of Girih tiling program, the program has been applied to math-gifted students and the program has been modified and complemented. This program which has been developed for math-gifted students is called 'Exploring the Secrets of Girih Hidden in Mosque Patterns'. The program was based on the Renzulli's three-part in-depth learning. The first part of the in-depth learning activity, as a research stage, is designed to examine Islamic patterns in various ways and get the gifted students to understand and have them motivated to learn the concept of the tiling, understanding the characteristics of Islamic patterns, investigating Islamic design, and experiencing the Girih tiles. The second part of the in-depth learning activity, as a discovery stage, is focused on investigating the mathematical features of the Girih tile, comparing Girih tiled patterns with non-Girih tiled ones, investigating the mathematical characteristics of the five Girih tiles, and filling out the blank of Islamic patterns. The third part of the in-depth learning activity, as an inquiry or a creative stage, is planned to show the students' mathematical creativity by thinking over different types of Girih tiling, making the students' own tile patterns, presenting artifacts and reflecting over production process. This program was applied to 6 students who were enrolled in an unified(math and science) gifted class of D elementary school in Daejeon. After analyzing the results produced by its application, the program was modified and complemented repeatedly. It is expected that this program and its materials used in this study will guide a direction of how to develop methodical materials for math-gifted education in elementary schools. This program is originally developed for gifted education in elementary schools, but for further study, it is hoped that this study and the program will be also utilized in the field of math-gifted or unified gifted education in secondary schools in connection with 'Penrose Tiling' or material of 'quasi-crystal'.