• BMB Reports
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• v.28 no.1
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• pp.51-56
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• 1995

Thioredoxin is a small redox protein with an active-site disulfide/dithiol, and is ubiquitous in bacteria, plants, and animals. To investigate the structure-function relationship of thioredoxin, the genes encoding Escherichia coli thioredoxin and Corynebacterium nephridii thioredoxin C3 were fused via a common restriction site in the nucleotide sequence coding for the active site of the proteins to generate two chimeric thioredoxins, designated E-C3(N to C-terminal) and C3-E. The hybrid thioredoxin genes were put under the T7 promoter and their productions were confirmed. The two hybrid thioredoxins complemented phenotypes of a thioredoxin-deficient E. coli strain. A strain containing the C3-E hybrid thioredoxin supported growth of the T7 phage, whereas a strain expressing the E-C3 hybrid thioredoxin did not. However, both hybrids supported growth of M13 phages. The two hybrid thioredoxins were also characterized in other aspects. Differences in activity between the hybrid thioredoxins were attributed to altered interactions of the N- and C-terminal domains of the molecule, which produced changes in the three-dimensional structure of the active site region.

• Asian Journal of Turfgrass Science
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• v.11 no.1
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• pp.59-72
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• 1997

The influence of respiration on photosythetic electron transport were investigated in the Wid type and psaB mutants from Syneehocystis sp. PCC6803. The amount of glucose uptake in the wild type was proportional to the glucose concentration added in wild type and less than that of psaB mutants in the dark. It was suggested that psaB mutants more depend on the glucose than the wild type. It was investigated how the activities of isocitrate dehydrogenase(IDH) and glucose-6-phos-phate dehydrogenase(G6PDH) were changed. The activities of IDH were very low. While, the ac-tivities of G6PDH were much higher than that of IDH. These results agree to the reports that ex-ogenous glucose was dismilated aerobically via Oxidative Pentose Phosphate Pathway in heterotrophic cyanobacteria. PsaB mutants showed high G6PDH activity in the presence of glucose as well as in the dark and high respiratory activities especially in the dark. It was also investigated how photosynthetic electron transport activities were changed. PsaB mutants showed higher photosynthetic electron tranasport activities than wild type in the presence of glucose as well as in the dark. In the results, it was proposed that photosynthetic electron transport between PS I and PS U was complemented by respiratory electron transport through the NADPH generated by Dxidative Pentose Phophate Pathway in psaB mutant from Synechocystis sp. PCC6803. Key words: Photosynthetic electron transport, Respiration, Synechoystis sp. PCC6803, psaB mutant, Glucose uptake, IDH, G6PDH, Respiratory electron transport activity.

• Knowledge Management Research
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• v.17 no.1
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• pp.49-72
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• 2016

Extant research on firm innovation productivity is limited in measuring the innovation productivity, in which they measured firm innovation productivity by using either inputs or outputs of innovation. The present study complemented the extant research by employing Data Envelopment Analysis (DEA) approach to measure firm innovation productivity. Furthermore, this paper examined the effects of firms' external knowledge search, as one of open innovation practices, on firm innovation productivity, for open innovation activities are regarded as an influencing factor on firm innovation productivity in the previous literatures. Using the data of the Korean Innovation Survey (KIS) of manufacturing industries conducted in 2008, this study developed hypotheses in which we considered not only two dimensions of external knowledge search (breadth and depth) but also two subtypes of external knowledge search (market-driven and science-driven). The results found that searching deeply and market-driven search are positively related to firm innovation productivity, but science-driven search is somewhat negatively related to firm innovation productivity. Furthermore, market-driven search can mitigate the negative effect of science-driven search on innovation productivity.

• Journal of International Academy of Physical Therapy Research
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• v.5 no.1
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• pp.641-646
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• 2014

The purpose of this study is to observe how balance exercise on an unstable platform and on a stable platform affects balance ability. The subjects were 35 adults in their 20s and were randomly assigned to a stable platform group and an unstable platform group. They performed balance exercise three times per week for six weeks. Balance exercise introduced by previous research was modified and complemented for use in this study. Balance ability of the subjects was measured through center of pressure(COP) area, medial-lateral displacement, and anterior-posterior displacement using a portable balance platform BT4. There was significant difference in the COP area between the unstable platform exercise group and the stable platform exercise group. In comparison in differences between the unstable platform exercise group and the stable platform exercise group after the exercise, there was significant difference in anterior-posterior movement. Therefore, exercise on an unstable platform is more effective than exercise on a stable platform in strengthening balance ability.

• International Journal of Industrial Entomology and Biomaterials
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• v.13 no.2
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• pp.85-95
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• 2006

Seveval Chinese and Japanese varieties with good characters were used in the breeding. After 5 years (15 generations), a pair of robust and high quality silkworm variety with NPV resistance was bred by means of a combination of crossing and pedigree selection complemented by the selection of NPV resistance. The variety was identified jointly nationwide in 2003 and 2004, and appraised by National Mulberry and Silkworm Appraising Committee. Results are as follows: its cocooning rate is over 93%, shell rate 23-25%, filament length 1200-1300 meters, reelability 75-88%, Length of non-broken cocoon filament 900-1100 meters, raw silk rate 17-19%, neatness 95-97 points, and cocoon crop, cocoon shell weight and raw silk weight per 10000 larvae is higher than those of the control variety by 7-10%, 14-19% and 14-18%, respectively. The variety is not only robust, resistant to high temperature and NPV, easy to rear, uniform in hatching, molting and maturing, but also lays more eggs, and its fecundity is high. It is suitable to rear in the Yangtze River Basin, the Yellow River basin and the Pearl River basin of China.

• Macromolecular Research
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• v.17 no.7
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• pp.533-537
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• 2009

This paper introduces a modified electro spinning system for biomedical wound-healing applications. The conventional electrospinning process requires a grounded electrode on which highly charged electro spun ultrafine fibers are deposited. Biomedical wound-healing membranes, however, require a very low charge and a low level of remnant solvent on the electrospun membrane, which the conventional process cannot provide. An electrohydrodynamic process complemented with field-controllable electrodes (an auxiliary electrode and guiding electrodes) and an air blowing system was used to produce a membrane, with a considerably reduced charge and low remnant solvent concentration compared to one fabricated using the conventional method. The membrane had a small average pore size (102 nm) and high porosity (85.1%) for prevention of bacterial contamination. In vivo tests on rats showed that these directly electro spun fibrous membranes produced using the modified electro spinning process supported the good healing of skin bums.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• v.9 no.1
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• pp.335-338
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• 2005

Though GF(2) CA can only handle data with bit units, GF(2$^{p}$) CA can handle data with byte units. In this paper we analyze the state-transition of nongroup cellular automata(CA) with a single attractor over GF(2$^{p}$). And we propose the constructing method of the state-transition diagram of a linear SACA over GF(2$^{p}$) by using the concept of basic path. Also we propose the state-transition diagram of the nonlinear complemented SACA by using the state-transition diagram of a linear SACA.

• Journal of Information Processing Systems
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• v.8 no.3
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• pp.521-538
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• 2012

As the knowledge society matures, not only distant, but also off-line universities are trying to provide learners with on-line educational contents. Particularly, high effectiveness of mobile devices for e-Learning has been demonstrated by the university sector, which uses distant learning that is based on blended learning. In this paper, we analyzed previous m-Learning scenarios and future technology prospects. Based on the proposed m-Learning scenario, we designed cellular phone-based educational contents and service structure, implemented m-Learning system, and analyzed m-Learning service satisfaction. The design principles of the m-Learning service are 1) to provide learners with m-Learning environment with both cellular phones and desktop computers; 2) to serve announcements, discussion boards, Q&A boards, course materials, and exercises on cellular phones and desktop computers; and 3) to serve learning activities like the reviewing of full lectures, discussions, and writing term papers using desktop computers and cellular phones. The m-Learning service was developed on a cellular phone that supports H.264 codex in 3G communication technology. Some of the functions of the m-Learning design principles are implemented in a 3G cellular phone. The contents of lectures are provided in the forms of video, text, audio, and video with text. One-way educational contents are complemented by exercises (quizzes).

• Journal of Korean Society of Steel Construction
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• v.20 no.1
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• pp.165-173
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• 2008

In order to promote the practicality of high-rise steel buildings, the development of structural system which have the better fire resistance, the changeable plan, and the quality control of construction with general composite beams is needed. In this research, new semi-slim floor which the defect of general slim floor was complemented was evaluated to investigate the concrete integration with slim-flor beam and the flexural performance. 5 simply supported semi-slim floor beam tests were performed with parameters; structural form of slab support beam, slab thickness, with or without web opening, and shear connection. Experimental results showed that all specimen s had good ductile behavior.

• Journal of Korean Clinical Nursing Research
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• v.16 no.3
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• pp.175-186
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• 2010

Purpose: Delirium can be a highly prevalent symptom in intensive care units but it may still be under-recognized despite its relation with inclined morbidity, mortality, cost, and readmission. Therefore, this study aimed to develop a protocol that covers risk factors and non-pharmacological interventions to prevent delirium in ICU patients. Methods: This study was conducted using methodological design, and it followed the Scottish Intercollegiate Guideline Network (SIGN) guideline development steps: 1) the scope of protocol was decided (population, intervention, comparison, and outcomes); 2) guidelines, systematic reviews, and protocols were reviewed and checked using methodology checklist; 3) the level of evidence and recommendation grades was assigned; 4) the appropriateness of recommendations was scored by experts; 5) the final protocol & algorithm was modified and complemented. Results: The evidence-based delirium prevention protocol was completed that includes predisposing factors, precipitating factors and recommendations with evidenced grades. Conclusion: This protocol can be used as a guide nurses in screening patients with high risk factors of delirium as well as in intervening the patients non-pharmacologically to prevent delirium.