• Title/Summary/Keyword: 1차계통

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Codon usage analysis of rice glutelin genes (쌀 저장 단백질 글루텔린 유전자 암호 분석)

  • Shin, Yun-Cheol;Kim, Ju-Kon;Nahm, Baek-Hie
    • Applied Biological Chemistry
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    • v.36 no.6
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    • pp.517-524
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    • 1993
  • To characterize glutelins, the most abundant storage protein in rice, 13 complete coding sequences of glutelin genes from the database were analyzed. According to the phylogenic analysis, these genes could be classified into 5 groups, Group I to V. The degrees of homology were calculated to be in the range of 90 to 60%, but the patterns of hydrophobicity were similar in all the groups. Also, each group was found to have similar amino acid composition with variations in lysine content from 2.5 to 3.6% due to the point mutation of arginine to lysine. The isoelectric points of mature proteins and their basic chains of all the groups showed the value of about 9.0 and 10.0, respectively, while the isoelectric points of acidic chains in these groups showed the distinct value of 6.6, 6.7, 7.2, 8.4 and 7.9. The plot of the fraction of G+C at synonimous site in codons (GC3s) against effective codon numbers suggest no major difference in translational efficiency in the expression of glutelin multigenes.

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A Study on Suppression of UT Grain Noise Using SSP MPO Algorithms (SSP MPO 알고리즘을 이용한 초음파 결정립 잡음 억제에 관한 연구)

  • Koo, Kil-Mo;Jun, Kye-Suk
    • The Journal of the Acoustical Society of Korea
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    • v.15 no.6
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    • pp.81-89
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    • 1996
  • It is very important for ultrasonic test method to evaluate the integrity of the class I components in nuclear power plants. However, as the rltrasonic test is affected by internal structures and configurations of test materials, backscattering, that is, time invariant noise is generated in large grain size materials. Due to the above reason, the received signal results in low signal to noise(S/N) ratio. Split spectrum processing(SSP) technique is effective to suppress the grain noise. The conventional SSP technique. however, has been applied to unique algorithm. This paper shows that MPO(minimization and polarity threshold) algorithm which two algorithms are applied simulatancously, was utilized, the signal processing time was shorten by using the new constant-Q SSP with the FIR filter which frequency to bandwidth ratio is constant and the optimum parameters were analysed for the signal processing to longitudinal wave and shear wave with the same requirements of inspection on nuclear power plant site. Moreover, the new ultrasonic test instrument, the reference block of the same product form and material specification, stainless stell test specimens and copper test specimens block of the same fabricated for the application of new SSP technique. As the result of experimental test with new ultrasonic test instrument and test specimens, the signal to noise ratio was improved by appying the new SSP technique.

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A Study on the Collaboration of the Function Analysis and Idea Creation Phase with Function Integration FAST(FI FAST) and Hierarchical Value Engineering Concept Modules(HVECM) (통합 기능계통도와 계층적 컨셉모듈을 활용한 기능분석단계와 창조단계의 연계성에 관한 고찰)

  • In, Chi-Sung;NamKung, In-Il;Hyun, Chang-Taek;Koo, Kyo-Jin
    • Proceedings of the Korean Institute Of Construction Engineering and Management
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    • 2006.11a
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    • pp.355-360
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    • 2006
  • Function analysis and idea creation phase in Value Engineering(VE) Process is applied to manufacturing Industry for developing functions for elements of products, and in construction come to use various components like process, size of project, spaces as well as function itself due to multi-functional aspect. For this reason, VE applications to the construction industry are considered to be less frequent and efficient than to the manufacturing. To resolve this problem, a new technique of Function Integration Fast(HI FAST) and Hierarchical Value Engineering Concept Modules(HVECM) was made for practical integration and sequence of VE job Plan. Two types of function would be defined In this method, first and second function, during the job Plan process, and two function champions be selected for next creation phase process. For verification of consecutiveness of function analysis and idea creation phase for successful VE project implementation, this methodology was tested under design phase in office and IT Multi-functional building project. Make sure the successful application of this process with this method, need to develop the talent of VE experts and team members for process of function analysis and creation phase.

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Isolation and Characterization of Bacillus Species Possessing Antifungal Activity against Ginseng Root Rot Pathogens (인삼 뿌리썩음병에 길항력이 있는 Bacillus 균의 분리 동정 및 특성 조사)

  • Kim, Byung-Yong;Ahn, Jae-Hyung;Weon, Hang-Yeon;Song, Jaekyeong;Kim, Sung-Il;Kim, Wan-Gyu
    • The Korean Journal of Pesticide Science
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    • v.16 no.4
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    • pp.357-363
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    • 2012
  • Ginseng (Panax ginseng C. A. Meyer) is an economically important crop in Korea. While the consumption of the crop is gradually increasing, the yield is decreasing due to the injury of continuous cultivation or infection of soil-borne fungal pathogens such as Cylindrocarpon destructans, Fusarium solani, Rhizoctonia solani and Sclerotinia nivalis. In order to find promising biocontrol agents, we have isolated 439 soil bacteria from ginseng cultivated soil and tested their antifungal activities against ginseng rot pathogens. Among them, 3 strains were finally selected and tested for the elucidation of their genetic and biochemical properties. They were identified as Bacillus amyloliquefaciens using phylogenetic analysis based on 16S rRNA gene sequences. Moreover, all selected strains showed positive reaction for PCR detection targeting biosynthetic gene sequences of iturin A and surfactin. The results provided promising evidences that the bacterial strains isolated from ginseng cultivated soil can be novel biocontrol agents for ginseng cultivaion.

Analytical Evaluation of Residual Stresses in Dissimilar Metal Weld for Cast Stainless Steel Pipe and Low-Alloy Steel Component Nozzle (스테인리스주강 배관과 저합금강 기기노즐 이종금속용접부 잔류응력의 해석적 평가)

  • Park, June-Soo;Song, Min-Seop;Kim, Jong-Soo;Kim, In-Yong;Yang, Jun-Seog
    • Proceedings of the KWS Conference
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    • 2009.11a
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    • pp.100-100
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    • 2009
  • This paper is concerned with numerical analyses of residual stresses in welds and material's susceptibility to stress corrosion cracking (SCC) for the primary piping system in nuclear power plants: Both the dissimilar metal weld (DMW) for stainless steel to low alloy steel joints and the similar metal weld (SMW) for forged stainless steel to cast stainless steel joints are considered. Thermal elasto-plastic analyses using the finite element method (FEM) are performed to predict residual stresses generated in fabrication welding and its related processes for both the DMW and SMW, including effects of quenching for cast stainless steel piping, machining of the DMW root, and grinding of the SMW root. As a result, the effect of quenching should be included in the evaluation of residual stresses in the SMW for the cast stainless steel piping. It is deemed that residual stresses in both the DMW and SMW would not affect the SCC susceptibility of the welds providing that the welding processes are completed without any weld repair on the inside wall of the joint. However, the grinding process if performed on the safe-end to piping weld, would produce a high level of residual stresses in the inner surface region and thus a stress improvement process (e.g. buffing) should be considered to reduce susceptibilities to SCC.

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Effects of Sodium Chloride Treatment on Seed Germination and Seedling Growth of Italian Ryegrass Cultivars (이탈리안 라이그라스 품종별 NaCl 처리가 발아 및 초기생육에 미치는 영향)

  • Lee, Sang-Hoon;Choi, Gi Jun;Lee, Dong-Gi;Mun, Jin-Yong;Kim, Ki-Yong;Ji, Hee Jung;Park, Hyung Soo;Lee, Ki-Won
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.34 no.2
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    • pp.108-113
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    • 2014
  • This study was conducted to evaluate the performance of Italian ryegrass cultivars for salt tolerance under in vitro condition. Italian ryegrass cultivars such as Greenfarm, Florida80, Kowinearly, and Hwasan101 were tested for their tolerance to various sodium chloride levels (0, 50, 150, 250, and 350 mM). The seed germination, growth, and activities of antioxidant enzymes were investigated under salt treatment. Physiological traits such as seed germination percentage, germination period, shoot and root length, and dry weight were suppressed under entire salt stress conditions. The results indicated that the highest germination percentage and shoot and root length were recorded at normal conditions. Increased sodium chloride levels caused a significant reduction in the seed germination and growth rate. Among the four tested cultivars, Italian ryegrass 'Hwasan101' could be considered as salt tolerant owing to its higher germination percentage, better seedling growth and antioxidant activities under salinity stress, whereas Greenfarm cultivar was more sensitive. The selection of Italian ryegrass cultivars for greater tolerance to saline environment would allow greater productivity from large saline lands.

Characteristics of Biocellulose by Gluconobacter uchimurae GYS15 (Gluconobacter uchimurae GYS15 균주로부터 생산되는 Biocellulose의 특성 확인)

  • Lee, Young Sun;Kim, Jae Young;Cha, Mi Yeon;Kang, Hee Cheol
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.42 no.3
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    • pp.247-255
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    • 2016
  • In order to select a strain that forms a Biocellulose (BC), strain producing acetic acid was selected from commercially available kombucha. Through SM broth it was confirmed that the strain is a gram negative bacteria in the form of rods having no motility through a phase contrast microscope. The result of phylogenetic inference analysis based on 16S rDNA sequence analysis for the identification of strains was most closely related to Gluconobacter uchimurae (G. uchimurae) and was named G. uchimurae GYS15 strain. The strain showed the highest degree of growth when cultured for 14 days under the conditions of pH 5 and $25^{\circ}C$. Moreover, it showed the highest degree of growth in a Glucose addition disaccharide as the optimum carbon source sucrose and fructose. Also, 0.5% NaCl, upon the addition of Malto extract, showed the highest degree of growth. Based on investigation by the optimum growth conditions to confirm the physical properties of BC obtained by culturing G. uchimurae GYS15 strains. The surface structure was observed through an scanning electron microscope (SEM) showed a high networks structure. It until $8.6{\pm}0.38$ times when the water holding capacity is re-absorbed and re-absorbed holding oil up to $6.6{\pm}0.51$ times confirmed. In conclusion, using these material properties, it was possible to confirm the possibility of a variety of cosmetic materials and mask pack materials.

Effects of Corn Distiller's Dried Grains with Solubles on Production Performance, Nutrient Digestibilities and Fecal Ammonia Emission in Laying Hens (옥수수주정박이 산란계의 생산성, 영양소이용율 및 분 암모니아 방출에 미치는 효과)

  • Shin, M.H.;Rew, H.J.;Lee, H.R.;Lee, S.K.;Lee, B.D.
    • Korean Journal of Agricultural Science
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    • v.35 no.2
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    • pp.137-145
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    • 2008
  • A layer feeding trial was conducted to investigate the effects of high quality corn distiller's dried grains with solubles(DDGS) on laying performance and nutrient metabolizabilities. A total of 216 Hy-line Brown layers, 23-wk of age, were employed in a 10-wk feeding trial consisting of three dietary treatments(0%, 10%, and 20% DDGS), and six replicates per treatment. All experimental diets were prepared as iso-protein(17%) and iso-calorie(2,780 kcal/kg). At the end of the trial, the effect of DDGS on fecal ammonia emission was also estimated. The use of DDGS up to 20% in layer diets did not affect the feed intake, laying rate, egg weight, and feed conversion ratio(P>0.05). The DM metabolizability decreased significantly(P<0.05) by the DDGS supplementation(P<0.05). The NFE metabolizability decreased gradually as the level of DDGS increased(P<0.05). The fecal ammonia emission from DDGS-fed birds tended to decrease as the storage days extended. In conclusion, albeit DDGS has some unfavorable effects on DM and NFE metabolizabilities, it could be included in layer diets up to 20% level without any harmful effects on laying performance.

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Introduction of Spring Cultivation of Onions by Adapting the Plug Seedling System in the Middle Region of Korean Peninsula (중부지방에서 플러그 육묘에 의한 양파의 춘파재배 가능성)

  • Lee, Jung-Soo;Seong, Ki-Cheol;Sin, Young-An;Ro, Hee-Myong;Um, Young-Cheol
    • Horticultural Science & Technology
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    • v.18 no.1
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    • pp.9-13
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    • 2000
  • Cultivation of onions in Korean peninsula is so restricted to Southern region, mainly due to inadequate air temperature for the growth of onions in this region. This sometimes resulted in unstable supply of onions. The fluctuation of market prices mainly results from the limited production of onions in such restricted areas. Onions are usually transplanted at the end of fall and cultivated until the beginning of next summer. This study was designed to examine the possibility of extending production region of onions by adopting the plug seedling system, and the effects of plug seedling age, transplanting date, plug cell volume, and variety on the yield of onions were investigated. Transplanting date (Mar. 15 or Apr. 15) did not affect the yield of onions. However, 60-day-old seedlings produced significantly greater bulb yield (107.2 ton/ha) than those of 30- or 90- day-old seedlings. Bulb yields of seedlings raised in 128 and 72 cell trays were 125.0 and 120.8 ton/ha, which were significantly greater than those in other cell trays. Yield of seedlings raised in 406 cell trays was the lowest. Seedlings of mid-late 'Chenjuwhang' yielded 130.5 Ton/ha, which was significantly higher than those of other varieties. Considering the rainy season during early summer, we suggest that seedlings of onions raised for 60 days and transplanted on Mar. 15 should be advantageous in producing of marketable bulbs and that an early 'Yongbongwhang' should be the best variety.

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A Light Pink Freesia 'Bolero' with Single Multi-flowering for Cut Flower (연분홍색 홑꽃 다화성 절화용 프리지아 신품종 '볼레로' 육성)

  • Cho, Hae Ryong;Rhee, Hye Kyung;Kim, Mi Seon;Park, Sang Kun;Shin, Hak Ki;Joung, Hyang Young;Lim, Jin Hee
    • FLOWER RESEARCH JOURNAL
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    • v.19 no.2
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    • pp.115-118
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    • 2011
  • A light pink single freesia (Freesia hybrida Hort.) 'Bolero' was developed from a cross of breeding line ('Yellow Ballet' and 'Yellow Dream') and 'Sandra' followed by seedling and line selections at the National Institute of Horticultural and Herbal Science (NIHHS), Rural Development Administration (RDA) in 2007. Growth and flowering characteristic tests were conducted from 2003 to 2006. 'Bolero' has single type flower with light pink color (RHS, R55B). It has multiple florets, stable flower shape, upright leaves and stalks. The average cut flower yield of 'Bolero' observed in the greenhouse from 2005 to 2007 was 7.5 cuttings per plant. It has good production of cormlets and vigorous growth. 'Bolero' could be cultured under greenhouse conditions in Korea.