• 한국축산식품학회지
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• 제42권1호
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• pp.61-72
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• 2022

Antioxidants present in fruits and vegetables have a potential to reduce disease risk, and increase the shelf life of food products by reducing lipid oxidation. The effect of marination with antioxidants-rich fruit juices on quality characteristics of vacuum-packed chicken wings were examined during frozen storage. Chicken wings were mixed separately with marinades containing pineapple juice, June plum juice, and mango juice and kept for 12 h and 24 h. Three best marination conditions were selected based on a sensory evaluation. Antioxidant activity and total phenolic content of fruit juices, and marinade uptake, and marinade loss of marinated chicken wings were determined. In addition, vacuum packed marinated chicken wings were tested for pH, water holding capacity (WHC), 2-thiobarbituric acid reactive substances (TBARS) value and antioxidant activity over a 4-wk frozen storage. The best sensory properties were reported from chicken wings marinated with pineapple juice for 24 h, mango juice for 24 h, and June plum juice for 12 h (p<0.05) compared to other marinade-time combinations. Mango juice showed the highest antioxidant activity (92.2%) and total phenolic content (38.45 ㎍/mL; p<0.05) compared to other fruit juices. The pH and WHC of vacuumpacked chicken wings were slightly decreased over the frozen storage (p<0.05). Moreover, chicken wings marinated with mango juice had the lowest TBARS values and the highest 2,2-diphenyl-1-picryl-hydrazyl-hydrate free radical scavenging activity. In conclusion, mango juice was selected among tested as the most effective marinade for enhancing the oxidative stability of lipid while maintaining the other meat quality traits of vacuum-packed chicken wings.

• Preventive Nutrition and Food Science
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• 제14권4호
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• pp.323-328
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• 2009

Cryotin F could be used for hydrolyzing shrimp byproducts into bioactive ingredients, which could be used as value-added products. The objective of this study was to investigate the optimum condition for antioxidative activities of the enzymatic hydrolysate produced with Cryotin F using response surface methodology with central composite rotatable design. Shrimp byproducts (shells and heads) were hydrolyzed with Cryotin F. The experimental ranges of the independent variables for 20 experimental runs were 28.2-61.8${^{\circ}C}$ reaction temperature, pH 6-10 and 0.5-5.5% enzyme concentration. The degree of hydrolysis for the reaction products was measured. Their antioxidative activities were measured using 1,1-diphenyl-2-picryl-hydrazyl (DPPH) scavenging activity and Fe-chelating activity. The experimental method with central composite rotatable design was well designed to investigate the optimum condition for biofunctional ingredients with antioxidative activities using Cryotin F because of their high R2 values of 0.97 and 0.95 for DPPH-scavenging activity and Fe-chelating activity, respectively. Change in enzyme concentration did not significantly affect their antioxidative activities (p<0.05). Both DPPH scavenging activity and chelating activity against Fe for the enzyme hydrolysates were more affected by the pH of enzyme hydrolysis than by their action temperature. DPPH-scavenging activity was higher at acidic pH than alkali pH, while chelating activity against Few was inversely affected. Hydrolysate of shrimp byproducts showed high antioxidative activities depending on the treatment condition, so the optimum treatment of enzymatic hydrolysate with Cryotin F and other proteases can be applied to shrimp byproducts (shells) and other protein sources for biofunctional ingredients.

• Nutritional Sciences
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• 제5권4호
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• pp.197-202
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• 2002

We compared the radical scavenging activity of flavonoids and their antioxidant effects on erythrocyte Na leak, platelet aggregation and TBARS (thiobarbituric acid reactive substance) production, using Sprague Dawley rats. The concentrations of flavonoids needed for scavenging radicals by 50% ($SC_{50}$) in 0.1mM DPPH (2,2 Diphenyl 1-picryl hydrazyl) were: Quercetin, 7.4/$\mu$M; Catechin, 10.6$\mu$M; Morin, 22$\mu$M; Hesperidin, 400uM; and Naringin, 3.95mM. Morin completed its antioxidant activity in 2 minutes, while catechin, hesperidin and naringin had slow but long lasting antioxidant activity. Whole blood platelet aggregation, when incubated with quercetin or catechin, was significantly decreased (P<0.05) compared with the control. Sodium leak in intact erythrocytes was significantly lower when incubated with quercetin, compared with other flavonoids (P<0.05). Morin, hesperidin and naringin somewhat increased Na leak in intact erythrocytes. Sodium leak in erythrocytes treated with phenazine methosulfate (PMS) was increased overall, but was not affected by flavonoids. Intracelluar Na and K were not affected by treatment with PMS. TBARS production in platelet rich plasma (PRP) was significantly lower (P<0.05) than the control when incubated with quercetin or hesperidin. PMS treatment caused an increase in TBARS production regardless of flavonoids. In the present study antioxidant effects of flavonoids were not well correlated with their radical scavenging activities, although quercetin, which showed the strongest radical scavenging activity, had the greatest antioxidant effect.

• 한국미생물·생명공학회지
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• 제40권4호
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• pp.333-338
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• 2012

지의류는 곰팡이, 조류 및 남조균류의 공생체이다. 본 연구팀은 최근 북극 지의류로부터 분리한 몇몇 미생물 종의 항산화 활성에 대하여 연구하였고, 그들의 높은 항산화 활성을 확인하였다. 본 연구에서는 Cladonia sp., Sterocaulon sp., Umbilicaria sp. 및 Cetraria sp. 총 4종류의 지의류로부터 5종의 미생물을 새롭게 분리하였고 배양 후, 다양한 용매 추출법으로 그들의 항산화능을 조사하였다. DPPH와 ABTS 자유 라디컬 소거능 측정법 및 FRAP 분석 등을 수행하였다. 또한 총 폴리페놀함량과 총플라보노이드 함량 분석 역시 수행하였다. 지의류 유래 미생물 배양 추출액 중, Burkholderia sordidicola S5-B(T) 유사 미생물 종의 에틸아세테이트 추출액은 DPPH 분석에서 대조군인 아스코르빈산 (51.3%)에 비해 72.9%로 높은 항산화 활성을 보였을 뿐만 아니라, 높은 플라보노이드와 폴리페놀 함량을 나타내었다. 결과적으로, 이러한 지의류 유래 미생물 종들은 잠재적으로 천연 항산화제의 원천소재로서 이용가능할 것이다.

• Journal of Applied Biological Chemistry
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• 제60권3호
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• pp.219-226
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• 2017

본 연구에서는 탁리산 열수추출물을 용매 분획하여 항산화, 주름억제, 미백효과에 대한 활성을 검증하여 화장품 소재로서 이용가능성을 연구하였다. 용매 분획물로 DPPH radical scavenge, ABTS radical scavenge, elastase, tyrosinase 저해활성을 확인하였고 에틸아세테이트 분획물(TW-EA)이 가장 높은 저해활성을 나타내었다. 세포내에서의 주름억제, 미백효과를 확인하기 위해서 western blot을 실시하였고 비교 검증을 위해 사용한 positive contol보다 TW-EA의 억제효과가 뛰어난 것을 확인하였다. 이러한 결과로 탁리산의 화장품소재로서 적용 가능성을 검증하였다.

• 한국약용작물학회지
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• 제27권4호
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• pp.259-270
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• 2019

Background: Eucommia ulmoides has long been used as an herbal medicine for the treatment of diabetes, hypertension and other diseases in many Asian countries. Methods and Results: This study aimed at evaluating the antioxidant and anti-inflammatory properties of its water (EU-DW, and REU-DW) and ethanol (EU-EtOH, and REU-EtOH) extracts, as well as those of non-roasted E. ulmoides (EU) and roasted EU (REU) cortex. The following were assessed in each extract: total phenolic and flavonoid contents, 1,1-diphenyl-2-picryl-hydrazyl (DPPH), 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), and nitrite radical scavenging activities, reducing power, DNA damage prevention activity, and nitric oxide (NO) inhibition activity. Both EU and REU extracts showed high phenolic and flavonoid content, dose-dependent DPPH radical scavenging capacity, high reducing activity, and considerable DNA damage prevention activity. EU extracts showed remarkable ABTS free radicals scavenging capacity. REU extracts showed a higher radical scavenging capacity and played an important role in inhibiting NO production. Conclusions: The results of this study suggested that aqueous and ethanol extracts of EU and REU possess antioxidant capacities, and prevent oxidative damage to DNA, probably owing to their phenolic and flavonoid content. Therefore, EU and REU could be candidates antioxidant supplements.

• KSBB Journal
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• 제25권3호
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• pp.244-250
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• 2010

약용식물인 도꼬마리 추출물을 사용하여 유산균을 이용한 단일 및 복합배양을 통하여 새로운 기능성 물질의 생산을 검토하였다. 도꼬마리 추출물의 유산발효 시 단일배양 결과에서 유산생산을 통해 대체로 잘 증식하는 것이 확인되었으며, H. pylori의 항균 활성은 단일배양보다 복합배양 시 항균활성이 아주 우수하였다. 항균성 물질의 분리는 $C_{18}$ Seppak cartridge에 흡착시켜 30% methanol 용출획분에서 항균성물질이 확인되었으며, TLC로 분석한 결과 $R_f$ 0.46의 위치에서 항균성 물질을 확인하였다. 도꼬마리 유산발효액의 항산화활성은 총 polyphenol 함량 및 radical 소거능을 검토하였으며, 그 결과 총 polyphenol 함량은 미발효시에 비해 도꼬마리 추출물의 저농도에서 단일배양의 경우 2~4배 증가하였으며, Lactobacillus brevis KCTC 3498과 Lactobacillus helveticus KCTC 3545를 복합배양한 경우 1 mg/mL 첨가농도에서 polyphenol이 가장 높게 나타났다. Radical 소거능은 도꼬마리 추출물을 0.1 및 0.5 mg/mL로 첨가하여 발효하였을 때 30~40%의 scavenging이 확인되어 발효를 하지않았을 때 (25%)보다 발효를 하였을 때 높아지는 것으로 나타났다. 특히 Lactobacillus casei KCTC 3109의 경우 0.1 mg/mL의 농도에서 70%의 scavenging을 나타내었다. 이들 결과로부터 도꼬마리 추출물의 유산발효는 미발효시에 비해 Helicobactor pylori에 대한 항균활성물질의 생산과 기능성물질인 항산화물질의 생산성이 우수하다고 사료되었으며 천연한약재의 발효를 통한 천연물 신약 등 신기능물질의 생산가능성을 확인하였다.

• Asian-Australasian Journal of Animal Sciences
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• 제27권12호
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• pp.1736-1743
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• 2014

This study was designed to test the effects of dietary distillers dried grain (DDG) level on the growth performance, feed utilization, body composition and antioxidant activity of juvenile red seabream (Pagrus major). Six isonitrogenous and isocaloric diets were formulated to contain 0%, 5%, 10%, 15%, 20%, and 25% DDG from rice (designated as DDG0, DDG5, DDG10, DDG15, DDG20, and DDG25), respectively. Juvenile red seabream averaging $10.1{\pm}0.05g$ were randomly distributed into 400-L tanks in a flow through systems. Three replicate groups of fish were fed one of the experimental diets to visual satiation two times a day for 10 weeks. Survival, weight gain, feed efficiency, protein efficiency ratio and hepatosomatic index of fish were not affected by dietary DDG levels (p>0.05). Proximate and amino acid composition of whole body in juvenile red seabream were not affected by dietary DDG levels (p>0.05). Plasma content of total protein, glucose, cholesterol, glutamic-pyruvic transaminase, phospholipid and triglyceride were not affected by dietary DDG levels (p>0.05). 1, 1-Diphenyl-2-picryl-hydrazyl radical and alkyl radical scavenging activities in plasma and liver of fish were not affected by dietary DDG levels (p>0.05). The results of this experiment suggest that DDG has the potential to replace plant origin ingredients such as wheat flour and corn gluten meal and could be used up to 25% in diet without incurring negative effects on the growth performance of juvenile red seabream.

• Journal of Microbiology and Biotechnology
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• 제20권11호
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• pp.1524-1528
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• 2010

The antioxidant properties of 46 lichen species, collected from the highly UV-exposed alpine areas of southwestern China, were evaluated for their potential therapeutic utilization. The anti-linoleic acid peroxidation activity, 1,1-diphenyl-2-picryl-hydrazyl (DPPH) scavenging activity, reducing power, and total phenolic contents were all assessed in vitro in the methanol extract of the lichens. A potent reducing power was detected in a number of the lichen extracts, when compared with butylated hydroxyanisole (BHA). In general, it was found that many of the lichens, with antioxidant properties, contained large quantities of phenolic content. Extracts of Peltigera praetextata and Sticta nylanderiana were found to exhibit the most potent activity in all of the antioxidant tests. In particular, extracts of S. nylanderiana displayed a 1.37 times greater anti-linoleic acid peroxidation activity, when compared with the ascorbic acid used as the positive control. S. nylanderiana also possessed the strongest free radical scavenging activity amongst all the tested species, with an inhibition rate of 90.4% at concentration of $330{\mu}g/ml$. Activity-guided bioautographic TLC and HPLC analyses were used to establish which compounds were responsible for the potent antioxidant activities of the S. nylanderiana extract. These analyses revealed lecanoric acid to be primarily responsible for the effective antioxidant properties of S. nylanderiana. Overall, these results have indicated that several highland lichens have the potential of being utilized as novel bioresources for naturally occurring antioxidant therapies.

• Fisheries and Aquatic Sciences
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• 제17권1호
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• pp.85-93
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• 2014

A 9-week feeding experiment was designed to test the effects of the dietary inclusion of distillers dried grain (DDG) on the growth performance, body composition, and antioxidant activity of juvenile rockfish Sebastes schlegeli. Five isonitrogenous and isocaloric diets were formulated to contain 0% DDG (DDG0), 7% and 14% DDG from rice (diets DDG-R7 and DDG-R14, respectively), as well as 7% and 14% DDG from rice and wheat flour (diets DDG-RW7 and DDG-RW14, respectively). Three replicate groups of juvenile rockfish averaging $68.0{\pm}0.4g$ were fed one of the diets to apparent satiation twice a day. Survival, weight gain, feed efficiency, and the protein efficiency ratio of fish were not affected by dietary DDG (P > 0.05). Proximate and amino acid compositions of the whole body were not affected by dietary DDG (P > 0.05). Plasma total protein, glucose, total cholesterol, glutamate oxaloacetate transaminase, glutamate pyruvate transaminase, phospholipid, and triglyceride were not affected by dietary DDG (P > 0.05). Radical scavenging activity on 1,1-diphenyl-2-picryl-hydrazyl (DPPH) in the plasma of rockfish fed the DDG-RW14 diet was higher than that of fish fed the DDG0 diet (P < 0.05). Hydroxyl radical scavenging activity in the liver of rockfish fed diets containing DDG was higher than that of fish fed the DDG0 diet (P < 0.05). Alkyl radical scavenging activity in the liver of rockfish fed the DDG-R7 diet was higher than that of fish fed the DDG0 diet (P < 0.05). These results suggest that DDG is a suitable ingredient for the partial replacement of wheat flour and can be used at a rate of up to 14% in the diet without incurring negative effects on the growth performance or body chemical composition of juvenile rockfish.