• Title/Summary/Keyword: 03A05

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New Species of the Genus Metschnikowia Isolated from Flowers in Indonesia, Metschnikowia cibodasensis sp. nov.

  • Sjamsuridzal, Wellyzar;Oetari, Ariyanti;Nakashima, Chiharu;Kanti, Atit;Saraswati, Rasti;Widyastuti, Yantyati;Ando, Katsuhiko
    • Journal of Microbiology and Biotechnology
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    • v.23 no.7
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    • pp.905-912
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    • 2013
  • A novel species, Metschnikowia cibodasensis, is proposed to accommodate eight strains (ID03-$0093^T$, ID03-0094, ID03-0095, ID03-0096, ID03-0097, ID03-0098, ID03-0099, and ID03-0109) isolated from flowers of Saurauia pendula, Berberis nepalensis, and Brunfelsia americana in Cibodas Botanical Garden, West Java, Indonesia. The type strain of M. cibodasensis is ID03-$0093^T$ (= NBRC $101693^T$ =UICC $Y-335^T$ = BTCC-$Y25^T$). The common features of M. cibodasensis are a spherical to ellipsoidopedunculate shaped ascus, which contains one or two needle-shaped ascospores, and lyse at maturity. Asci generally develop directly from vegetative cells but sometimes from chlamydospores. The neighbor-joining tree based on the D1/D2 domain of nuclear large subunit (nLSU) ribosomal DNA sequences strongly supports that M. cibodasensis (eight strains) and its closest teleomorphic species, M. reukaufii, are different species by a 100% bootstrap value. The type strain of M. cibodasensis, ID03-$0093^T$, differed from M. reukaufii NBRC $1679^T$ by six nt (five substitutions and one deletion) in their D1/D2 region of nLSU rDNA, and by 18 nt (five deletions, four insertions, and nine substitutions) in their internal transcribed spacer regions of rDNA, respectively. Four strains representative of M. cibodasensis (ID03-$0093^T$, ID03-0095, ID03-0096, and ID03-0099) showed a mol% G+C content of $44.05{\pm}0.25%$, whereas that of M. reukaufii NBRC $1679^T$ was 41.3%. The low value of DNA-DNA homology (5-16%) in four strains of M. cibodasensis and M. reukaufii NBRC $1679^T$ strongly supported that these strains represent a distinct species.

Knowledge, Attitude, Performance on the Hand Washing by Dental Hygiene Students (치위생과 학생의 손 씻기에 대한 지식, 태도, 수행도)

  • Lee, Bo-Ram;Han, Ok-Sung
    • Journal of dental hygiene science
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    • v.15 no.2
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    • pp.166-171
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    • 2015
  • This study was conducted to assess knowledge, attitude, and performance of dental hygiene students on the hand washing. We surveyed 288 students attending the department of dental hygiene at K Universities and H Universities in Gwangju during the period from September 5 to November 20, 2014. The obtained data were analysed using SPSS version 20.0. According to the general characteristics, the hand washing knowledge of the third year students was highest with 4.72. At the question on the change of awareness after the education of hand washing, the hand washing performance showed that 4.17 for 'yes' was higher than 4.03 for 'no'. There was negatively correlated between knowledge and attitude (r=-0.020, p>0.05). The performance was positively correlated with attitude (r=0.161, p<0.05). There is a need to develop a continuous education program for finding out the right way to hand washing, which is able to enhance knowledge, attitude, and performance on hand washing.

Effects of Dietary Chelated Fe and Yeast Fe on Growth Performance and Body Composition in Broiler Chicks (유기태 철과 효모철의 첨가급여가 육계의 성장 및 체조성에 미치는 영향)

  • Na S.J.;Oh J.I.;Uuganbayar D.;Jung D.K.;Kim H.Y.;Moon S.T.;Yang C.J.
    • Korean Journal of Poultry Science
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    • v.33 no.2
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    • pp.113-119
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    • 2006
  • This study was carried out to investigate the effects of dietary Saccharomyces cerevisiae (wild yeast mutant) and Saccharomyces cerevisiae hFeHLC (ferritin containing yeast) and chelated Fe on growth performance and body composition of broiler chicks. A total of 252 (1-day old) 'Ross' broiler chicks were allotted to seven dietary treatments: I) control without iron supplementation, 2) 0.1% wild yeast mutant (YM03), 3) 1.0% wild yeast mutant, 4) 0.1% ferritin containing yeast (YF04), 5) 1.0% ferritin containing yeast, 6) 0.01% chelated Fe and 7) 0.1% chelated Fe. The highest weight gain obtained from broilers fed 0.01% chelated Fe was 2,121g, the lowest weight gain obtained from broilers fed control diet was 2.087g. The growth performance results were not significantly different in weight gain, feed intake and feed conversion ratio of broilers diets containing wild yeast, ferritin containing yeast and chelated Fe supplementation and control diets (P>0.05). The lowest iron content in meat was for control 30.51 mg/dL and the highest Fe content was for 0.1% ferritin containing yeast 41.58 mg/dL, but these were not significantly different (P>0.05).

The Effect of Coloring Food Additives on the ${\alpha}$-Chymotrypsin Activity (식품착색료가 ${\alpha}$-Chymotrypsin 작용에 미치는 영향)

  • Choi Cheong;Kim, Sang Ok
    • Journal of the Korean Chemical Society
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    • v.21 no.6
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    • pp.445-448
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    • 1977
  • This study was carried out to understand the activity of ${\alpha}$-chymotrypsin, a proteolytic enzyme, to a oligopeptide in the presence of various coloring food additives. 1. The melting point of synthetic oligopeptide, Asp-Arg-Val-Tyr-Ile-His-Pro-D-Ala, ((8-D-Ala) angiotensin Ⅱ) was 210∼$212^{\circ}C$. Chemical formula and molecular weight were $C_{44}H_{67}N_{13}O_{12}{\cdot}2CH_3COOH{\cdot}H_2O$ and 970.08, respectively. 2.The amino acid rations by acid hydrolysis were Asp : 1.01, Arg : 1.03, Val : 1.00, Tyr :40.94, Ile : 1.00, His : 1.05, Pro : 1.04, D-Ala : 1.03. 3. ${\alpha}$-Chymotrypsin cleaved the oligopeptide bond between tyrosine and isoleucine (Tyr-Ile). 4. The addition of food coloring additives as determined by paper chromatogram, did not influence the inhibitory activity of ${\alpha}$-chymotrypsin on oligopeptide, (8-D-Ala) angiotensin II.

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Variance component analysis of growth and production traits in Vanaraja male line chickens using animal model

  • Ullengala, Rajkumar;Prince, L. Leslie Leo;Paswan, Chandan;Haunshi, Santosh;Chatterjee, Rudranath
    • Animal Bioscience
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    • v.34 no.4
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    • pp.471-481
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    • 2021
  • Objective: A comprehensive study was conducted to study the effects of partition of variance on accuracy of genetic parameters and genetic trends of economic traits in Vanaraja male line/project directorate-1 (PD-1) chicken. Methods: Variance component analysis utilizing restricted maximum likelihood animal model was carried out with five generations data to delineate the population status, direct additive, maternal genetic, permanent environmental effects, besides genetic trends and performance of economic traits in PD-1 chickens. Genetic trend was estimated by regression of the estimated average breeding values (BV) on generations. Results: The body weight (BW) and shank length (SL) varied significantly (p≤0.01) among the generations, hatches and sexes. The least squares mean of SL at six weeks, the primary trait was 77.44±0.05 mm. All the production traits, viz., BWs, age at sexual maturity, egg production (EP) and egg weight were significantly influenced by generation. Model four with additive, maternal permanent environmental and residual effects was the best model for juvenile growth traits, except for zero-day BW. The heritability estimates for BW and SL at six weeks (SL6) were 0.20±0.03 and 0.17±0.03, respectively. The BV of SL6 in the population increased linearly from 0.03 to 3.62 mm due to selection. Genetic trend was significant (p≤0.05) for SL6, BW6, and production traits. The average genetic gain of EP40 for each generation was significant (p≤0.05) with an average increase of 0.38 eggs per generation. The average inbreeding coefficient was 0.02 in PD-1 line. Conclusion: The population was in ideal condition with negligible inbreeding and the selection was quite effective with significant genetic gains in each generation for primary trait of selection. The animal model minimized the over-estimation of genetic parameters and improved the accuracy of the BV, thus enabling the breeder to select the suitable breeding strategy for genetic improvement.

An Experimental Study on the Determination of Minimum Response Concentration of Inorganic Pollutants in Tap Water (수돗물에서 무기 오염물질 최소 반응 농도 결정의 실험적 고찰)

  • Yoon, Sukmin;Kim, Seong-Su;Chea, Seon-Ha;Park, No-Suk
    • Journal of Korean Society of Environmental Engineers
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    • v.39 no.4
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    • pp.208-213
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    • 2017
  • In this study, four inorganic pollutants (cadmium, chromium, manganese, lead), that could cause contamination events in drinking water distribution system, were selected and batch tests were carried out to determine the "minimum response concentration (MRC)", a part of Korean Contamination Warning system establishment. As the results, the minimum response concentration of cadmium was found to be 0.05 to 0.08 mg/L (0.005 mg/L : water quality standard) and that of chrome was 0.03 mg/L (0.05 mg/L). And the minimum reaction concentration was 0.005 mg/L for manganese (0.05 mg / L for water quality) and 0.02~0.08 mg/L for lead (0.01 mg/L).

Effect of Perenniporia fraxinea on Eliminating Urushiol from Rhus verniciflua Stokes Stem Bark (아까시재목버섯이 옻나무 껍질의 urushiol 제거에 미치는 영향)

  • Lee, Ji-Hyun;Jeong, Seok-Tae;Kang, Ji-Eun;Choi, Han-Seok
    • The Korean Journal of Mycology
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    • v.47 no.4
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    • pp.347-357
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    • 2019
  • This study evaluated the raw materials for spawning Perenniporia fraxinea, to eliminate urushiol. The growth rates of spawns on grains of millet, brown rice, and wheat were 4.92±0.05, 2.20±0.03, and 1.93±0.03 mm/day, respectively, and the laccase activity was 0.86±0.02, 0.04±0.01, and 0.01±0.00 U/mL, respectively. These observations revealed millet as the most appropriate grain for spawn production in terms of growth rate and enzyme activity. Inoculation of lacquer tree (Rhus verniciflua Stokes) stem bark with millet spawns of P. fraxinea resulted in a reduction of its urushiol contents, up to 86.6% on the third day and up to 98.5% on the seventh day. The optimal period of cultivation to eliminate urushiol was three days with 68% of residual flavonoids and 42% of phenolic components. When compared to the product cultivated from liquid spawn, the millet spawn reduced the cultivation period from 10 days to 3 days for eliminating urushiol.

Development of Temporary Preservation Method for Small Scale Dairy Farm Milk by $H_2O$$_2$ Catalase Treatment (Part 1) Bactericidal Effect of Hydrogen Peroxide and Its Stability in Milk ($H_2O$$_2$-Catalase처리에 의한 소규모 목장우유의 일시적 보존법의 개발 (제1보) 우유에 있어서 과산화수소의 살균효과 및 안정성)

  • Park, I.S.;Pack, M.Y.
    • Microbiology and Biotechnology Letters
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    • v.5 no.3
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    • pp.113-118
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    • 1977
  • Into the precontaminated farm milk hydrogen peroxide ($H_2O$$_2$) was added at the concentrations ranging from 0.01% to 0.05% and kept at 3$0^{\circ}C$ for 16 hours with periodical determinations for viable counts, residual $H_2O$$_2$, and lactic acid. Under the tested conditions the initial level of contaminated bacteria could be arrested from growing at least for 8, 12, and 16 hours by treating the milk with 0.01, 0.02. and 0.03 per cent of $H_2O$$_2$, respectively. Furthermore, when the $H_2O$$_2$concentrations ware limited within the level of 0.03 Per cent the added $H_2O$$_2$was completely decomposed within 12 hours without the aid of external catalase and the decomposition time decreased in parallel with the $H_2O$$_2$ concentrations. A safer use of $H_2O$$_2$for preserving farm milk temporarily by limiting its concentration has been discussed.

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A Study on Body Composition and IADL of the Elderly according to the Sex (성별에 따른 노인의 신체조성과 IADL에 관한 연구)

  • Um, Ki-Mai;Yang, Yoon-Kwon
    • Journal of Korean Physical Therapy Science
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    • v.7 no.1
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    • pp.321-331
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    • 2000
  • The purpose of this study was to investigate the difference of body composition and IADL of between male elder and female elder. (This study consisted of elder male(n=10) and elder female(n=10),) The mean age of elder male and female was 67.20, 67.50 years. The data were analyzed with t-test, using SPSS PC+ program. Body composition was measured using Inbody 2.0(seoul, korea) of segmental multi-frequency impedance analyzer technique. IADL was measured with Philadelphia Geriatric Center IADL. The result of this study were the following: 1. The difference of Body composition 1) The %fat of elder female was significantly(p<0.05) higher than elder male by 11.48%. 2) The fat mass(kg) of elder female was significantly(p<0.05) higher than elder male by 6.28kg. 3) The free fat mass(kg) of elder male was significantly(p<0.05) higher than elder female by 9.03kg. 4) The muscle mass(kg) of elder male was significantly(p<0.05) higher than elder female by 8.63kg. 5) The WHR(%) elder female was no significantly higher than elder male by 0.03%. 6) The TBW(l) of elder male was significantly(p<0.05) higher than elder female by 6.321. The factor of significant difference of between elder male and female was %fat, fat mass(kg), free fat mass(kg), total body water(l). 2. The difference of IADL 1) IADL of elder male was no significantly higher than elder female by 1.20. As a result of this study, optimal body composition group was elder male group. Optimal body composition of elder had improved IADL. In addition to, this result of this study, it can suggested the consideration of the Health promotion program for elder.

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A Study on Optimized MRI Fat-Saturation Technique for Brachial Plexus Patients : Focused on SPAIR and STIR Fat-Saturation (상완신경총 환자에서 최적의 자기공명영상 지방소거 기법에 관한 연구 : SPAIR, STIR 지방소거 중심으로)

  • Goo, Eunhoe
    • Journal of the Korean Society of Radiology
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    • v.8 no.5
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    • pp.271-278
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    • 2014
  • The purpose of this study is to know optimized fat suppression techniques for brachial plexus compared with STIR and SPAIR T1, T2 techniques. A total of 30 normal volunteers without brachial plexus disease were studied on a 3.0 T MRI scanner. As an analytical method, SNR, CNR, 4-point grading scale were evaluated by using three pulse sequences. As a quantitative analysis, the SNR, CNR for SPAIR T1 technique provided high value in branchial plexus roots (03.07, -2.25), branchial plexus trunks(06.70, 36.31)(p<0.05). As a qualitative Analysis, The visibility for delineation of brachial plexus, fat suppression, artifact was significantly better on SPAIR T1(3.2, 3.6, 3.4) technique(p<0.05).