• 제목/요약/키워드: 03-03

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An ${\beta}$-1,4-Xylanase with Exo-Enzyme Activity Produced by Paenibacillus xylanilyticus KJ-03 and Its Cloning and Characterization

  • Park, Dong-Ju;Lee, Yong-Suk;Chang, Jie;Fang, Shu-Jun;Choi, Yong-Lark
    • Journal of Microbiology and Biotechnology
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    • 제23권3호
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    • pp.397-404
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    • 2013
  • Paenibacillus xylanilyticus KJ-03 was isolated from soil samples obtained from a field with Amorphophallus konjac plants. A gene encoding xylanase was isolated from KJ-03 and cloned using a fosmid library. The xynA gene encodes xylanase; it consists of 1,035 bp and encodes 345 amino acids. The amino acid sequence deduced from the P. xylanilyticus KJ-03 xylanase showed 81% and 69% identities with those deduced from the P. polymyxa E681 and Paenibacillus sp. HPL-001 xylanases, respectively. The xynA gene comprises a single domain, consisting of a catalytic domain of the glycosyl hydrolase (GH) 10 family. The xynA gene was expressed in Escherichia coli BL21 (trxB), and the recombinant xylanase was purified by Niaffinity chromatography. The purified xylanase showed optimum activity with birchwood xylan as a substrate at $40^{\circ}C$ and pH 7.4. Treatment with $Mg^{2+}$ and $Li^+$ showed a slight decrease in XynA activity; however, treatment with 5 mM $Cu^{2+}$ completely inhibited its activity. The results of the thin layer chromatography analysis indicated that the major hydrolysis product was xylobiose and small amounts of xylose and xylotriose. XynA showed increased activity with oat spelt xylan and birchwood xylan, but showed only slight activity with locust bean gum.

(1-x)MgxSr$TiO_3$(x=0.03~0.04) 세라믹스의 마이크로파 유전특성 (Microwave Dielectric Properties of the (1-x)MgxSr$TiO_3$(x=0.03~0.04) ceramics)

  • 최의선;이문기;류기원;배선기;이영희
    • 한국전기전자재료학회:학술대회논문집
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    • 한국전기전자재료학회 2000년도 추계학술대회 논문집
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    • pp.547-550
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    • 2000
  • The (1-x)MgTiO$_3$-xSrTiO$_3$(x=0.03~0.04) ceramics were fabricated by the conventional mixed oxide method. The sintering temperature and time were 125$0^{\circ}C$~135$0^{\circ}C$, 2hr., respectively. From the X-ray diffraction patterns, it was found that the perovskite SrTiO$_3$ and ilmenite MgTiO$_3$ structures coexisted in the (1-x)MgTiO$_3$-xSrTiO$_3$(x=0.03~0.04) ceramics. The dielectric constant($\varepsilon$$_{r}$) was increased with addition of SrTiO$_3$. The temperature coefficient of resonant frequency($\tau$$_{f}$) was gradually varied from negative value to the positive value with increasing the SrTiO$_3$. The negative temperature coefficient of resonant frequency of the magnesium titanate was adjusted to near zero at x=0.036, where the dielectric constant, quality factor, and $\tau$$_{f}$ were 20.65, 95120, and +1.3ppm/$^{\circ}C$, respectively. The temperature stability of qualify factor in (1-x)MgTiO$_3$-xSrTiO$_3$(x=0.03~0.04) ceramics increased as the amount of MgTiO$_3$./TEX>.

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남해지역 마늘종의 이화학적 특성 (Physicochemical characteristics of garlic (Allium sativum L.) shoots from different areas in Namhae)

  • 윤환식;강민정;황초롱;심혜진;김경민;신정혜
    • 한국식품저장유통학회지
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    • 제21권3호
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    • pp.321-327
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    • 2014
  • 마늘종의 주요 성분 함량에 대한 기초자료를 확보하고자 마늘의 주산지인 남해군 8개 지역에서 마늘종을 수거하여 비교 분석하였다. 수분은 $80.15{\pm}0.37{\sim}83.87{\pm}0.15g/100g$, 조단백 및 조지방은 각각 $1.59{\pm}0.02{\sim}2.25{\pm}0.09g/100g$$0.35{\pm}0.01{\sim}0.48{\pm}0.03g/100g$이었으며 회분은 $0.65{\pm}0.03{\sim}0.71{\pm}0.03g/100g$이었다. 총페놀 및 플라보노이드 함량은 각각 $33.02{\pm}1.78{\sim}58.41{\pm}1.08mg/100g$$11.60{\pm}3.36{\sim}23.95{\pm}2.04mg/100g$이었다. 총 클로로필 함량은 이동면 지역의 마늘종에서 $170.58{\pm}1.04{\mu}g/g$으로 가장 높았고 창선면 지역의 마늘종은 $116.65{\pm}0.00{\mu}g/g$으로 가장 낮았다. 무기물은 총 8종이 검출되었으며, 검출된 무기물 중 칼륨($7257.0{\pm}87.9{\sim}10070.0{\pm}9.7mg/kg$)의 함량이 가장 높았고, 총량은 이동면과 남해읍 지역의 마늘종이 각각 21,163.7 mg/kg과 20,608.3 mg/kg으로 타 지역산에 비해 높은 함량이었다. 알리신 함량은 $78.03{\pm}6.15{\sim}203.43{\pm}18.47mg/kg$으로 재배지역에 따라 함량 차이가 컸으며, 함황화합물 5종을 분석한 결과 diallyl trisulfide가 약 70%로 가장 함유 비율이 높았는데, 이동면 지역 마늘종에서 $427.03{\pm}27.72mg/kg$으로 가장 높은 함량이었다. 이상의 결과를 종합하여 볼 때 지리적 환경이 유사한 남해군내 8개 지역에서 재배된 마늘종의 성분간 함량 차이는 시비량, 일조량 등의 재배 환경과 재배품종 및 재배기술에 의한 것으로 추정되며, 마늘종 재배지역간의 함량차이는 있으나 마늘의 유효물질을 함유하고 있어 식품소재로서 활용 가치가 우수할 것으로 판단된다.

Ag-$Bi_{1.84}Pb_{0.34}Sr_{1.91}Ca_{2.03}Cu_{3.06}O_{10+{\delta}}$ 복합체의 미세구조와 초전도특성 (Microstructure and Superconducting Properties of Ag-$Bi_{1.84}Pb_{0.34}Sr_{1.91}Ca_{2.03}Cu_{3.06}O_{10+{\delta}}$ Composites)

  • 이민수
    • 대한전기학회논문지:전기물성ㆍ응용부문C
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    • 제52권6호
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    • pp.249-256
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    • 2003
  • Samples with the nominal composition, Ag-$Bi_{1.84}Pb_{0.34}Sr_{1.91}Ca_{2.03}Cu_{3.06}O_{10+{\delta}}$ high $T_{c}$ superconductors containing Ag as an additive were fabricated by a solid-state reaction method. Samples with Ag of 10 wt%, 30 wt%, and 50 wt% each were sintered at $860^{\circ}C$~$870^{\circ}C$ for 24 hours. The structural characteristics, the microstructures and the critical temperature with respect to the each samples were investigated by XRD, four-prove methode, SEM and EDS respectively. The $T_{c}\;^{zero}$ of the sample with the 50 wt% Ag additive at the surface showed 94 K.

60년대부터의 수도권 시정 변화 특성연구 (Visibility Trend in the Seoul Metropolitan area since 1960s)

  • 박한석;김경원;김영준;방소영
    • 한국대기환경학회:학술대회논문집
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    • 한국대기환경학회 2003년도 춘계학술대회 논문집
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    • pp.293-294
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    • 2003
  • 국내의 시정측정은 목측에 의해서 이루어진다. 기상청에서는 1940년대부터 목측을 통해서 시정의 변화를 관측해 왔으며, 본 연구에서는 서울과 인천을 중심으로 한 수도권의 시정 변화를 상대습도의 영향을 고려하여 분석하였다. 초기(1940년- 1964년)의 목측은 숙련된 사람이 매일 03, 09, 15시의 3번을 관측하였고, 65년부터 82년 4월까지는 03, 09, 15, 21시의 4번 관측을 하였으며, 82년 5월부터는 3시간 간격으로 매일 8번(03, 06, 09, 12, 15, 18, 21, 24)의 관측이 이루어졌다. 야간시정은 주간과 같은 밝기로 가정하여 목표물을 확인하거나, 집광되지 않은 백색의 전등빛을 이용하는 식별법이다. (중략)

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Ni-Co 합금강의 기계적 특성에 대한 탄소함량의 영향 (Influence of Carbon Content on the Mechanical Properties of the Ni-Co Alloy)

  • 장경천;국중민;정장만;권택용;최병기
    • 한국공작기계학회:학술대회논문집
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    • 한국공작기계학회 2004년도 춘계학술대회 논문집
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    • pp.129-135
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    • 2004
  • This study was to evaluate the effect of carbon content on metallic change and fatigue characteristics with Fe-29% Ni-17% Co, low heat expansion alloy, widely using electronic components, precision machines, and sealing with glass and metal etc. The steels were fabricated with variation of carbon content, 0, 0.03, 0.06, 0.1, and 0.20% with VIM and tensile test and fatigue test were performed to achieve the above purpose. The more carbon content, the higher hardness value and yield strength. But elongation of 0.03%C, 0.06%C, and 0.10%C specimen decreased about 2.2%, 1.5% and 0.8% respectively mote than that of the base metal. Especially the strength and elongation of 0.20%C specimen increased simultaneously about 14.4% and 7.5%. Fatigue life of 0.03%C specimen decreased but the more carbon content, the higher fatigue life over 0.06% carbon content more than that of base metal.

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Improvement of Motor Behavior of Parkinson's Disease Animal Model by Nurr1 Transfected Human Embryonic Stem Cells

  • Lee, Chang-Hyun;Cho, Hwang-Yun;Kim, Yong-Sik;Kim, Eun-Young;Lee, Won-Don;Park, Sepill;Lim, Jin-Ho
    • 한국동물번식학회:학술대회논문집
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    • 한국동물번식학회 2004년도 춘계학술발표대회
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    • pp.274-274
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    • 2004
  • The purpose of this study is to evaluate the efficacy of in vitro differentiated human embryonic stem (MB03) cells expressing Nurr1 in relief of symptomatic motor behavior of Parkinson's disease (PD) animal models. MB03 cell was genetically modified to express Nurr1 protein (Nr#24/MB03) and was induced to differentiate according to 2- /4+ protocol using retinoic acid and ascorbic acid. (omitted)

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청색 $\textrm{Ca}\textrm({Al}_{0.97}\textrm{B}_{0.03})_2\textrm{O}_4$: $\textrm{Eu}^{+2},\;\textrm{Nd}^{+3}$ 형광체의 잔광 특성에 관한 연구 (A Study on the Phosphorescent Characteristics of Blue $\textrm{Ca}\textrm({Al}_{0.97}\textrm{B}_{0.03})_2\textrm{O}_4$: $\textrm{Eu}^{+2},\;\textrm{Nd}^{+3}$ Phosphor)

  • 김대수;이임렬
    • 한국재료학회지
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    • 제9권1호
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    • pp.3-7
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    • 1999
  • The change of phosphorescent property with chemical modification and co-doping of Nd as an auxiliary activator in CaAl$_2$$O_4$: Eu\ulcorner, Nd\ulcorner phosphor, employing Nd\ulcornerion(0.6%) and an co-activator maintained its afterglow for 4.5 hours. And also the initial persistent brightness and phosphorescent property were further improved both by substituting Al by B atoms and incorporation of Nd\ulcorner ion. It was found that the persistent time was 30 hours for&Ca(Al_{0.97}\;B_{0.03}) $_2$$O_4$:EU\ulcorner(0.3%), Nd\ulcorner(0.6%) phosphor.

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2차원 격자 오차 데이터 기반의 선형 보정 함수들을 이용한 적외선 레인지 파인더 PBS-03JN의 보정 (alibration of Infra-red Range Finder PBS-03JN Using Piecewise Linear Function Based on 2-D Grid Error)

  • 김진백;김병국
    • 제어로봇시스템학회논문지
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    • 제17권9호
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    • pp.922-931
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    • 2011
  • An efficient calibration algorithm for mobile robot localization using infrared range finder is proposed. A calibration is important to guarantee the performance of other algorithms which use sensor data because it is pre-process. We experimentally found that the infrared range finder PBS-03JN has error characteristics depending on both distance and scan angle. After obtaining 2-D grid error characteristic data on distance and scan angle, we proposed a simple and efficient calibration algorithm with a 2-D piecewise linear function set. The performance of our proposed calibration algorithm is verified by experiments and simulation.

Visual Perception for TCO'03 Angular-Dependence Luminance Uniformity of TFT-LCDs

  • Hsu, Yu-Ju;Chen, Hsin-Ming;Cheng, Yung-Chiang
    • 한국정보디스플레이학회:학술대회논문집
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    • 한국정보디스플레이학회 2006년도 6th International Meeting on Information Display
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    • pp.671-674
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    • 2006
  • Due to the stricter requests of the human perception of images, many industrial standards such as TCO'03 have been made to survey the Flat Panel Displays (FPD) nowadays. The angular-dependence luminance uniformity is an important item to evaluate the performance of FPD. In this article, we focus on the above test item base on TCO'03 standard in the case of vertical direction of ${\pm}15^{\circ}$. With controlling the driving voltage toward the liquid crystals of TN-type TFT-LCD, there exists a voltage-driving range which can achieve the value of the $L_{max}/L_{min}$ at ${\pm}15^{\circ}\;{\leq}1.7$ readily. Both experiment and simulation have been well analysis.

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