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Ecological Characteristics of Trioza ukogi (Shinji) (Homoptera: Triozidae) in Korea (한국산 오갈피나무이(매미목: 창나무이과)의 생태 특성)

  • Won, Dae-Sung;Park, Il-Kwon;Kim, Chul-Su;Shin, Sang-Chul;Kim, Jong-Kuk
    • Journal of Korean Society of Forest Science
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    • v.96 no.6
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    • pp.750-755
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    • 2007
  • This study was investigate to ecological characteristic of Trioza ukogi (shinji) on the Acanthopanax senticosus for. inermis Harms during 2004-2005 in Korea. The sizes of Trioza ukogi (shinji) were $0.40{\pm}0.03mm$ in eggs, $0.36{\pm}0.03mm$ in 1st instar nymphs, $2.50{\pm}0.25mm$ in mature nymphs and $5.17{\pm}0.28mm$ in adults (to tip of folded wings), respectively. T. ukogi has two generations per year. First generation appeared from mid-July to late August with peak in early August. Second generation appeared from mid-September to mid-October with peak in late September. Overwintering adults move to host tree and mate from the early April to late May with peak in late April. Overwintering female laid on the new leaves and first generation prefers to lay on the seeds. The number of ovarian eggs was 5$57.5{\pm}23$.

Production and Characterization of Thermostable Protease from Bacillus licheniformis Isolated from Korean Traditional Soybean Paste (재래식 된장에서 분리된 Bacillus licheniformis의 내열성 Protease 특성과 생산성)

  • Bae, Young Eun;Yoon, Ki-Hong
    • Korean Journal of Microbiology
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    • v.48 no.4
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    • pp.298-304
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    • 2012
  • Among 63 Bacillus strains grown at $60^{\circ}C$ from sixteen samples of homemade Korean soybean paste, one strain was selected for producing the thermostable protease. The isolate has been identified as Bacillus licheniformis on the basis of its 16S rDNA sequence, morphology and biochemical properties. Culture filtrate of the isolate showed maximal protease activity at the reaction condition of $60-65^{\circ}C$ and pH 11. The culture filtrate retained more than 87% of initial protease activity after incubation for 30 min at $60^{\circ}C$ without substrate. In order to develop the medium composition, effects of ingredients including nitrogen sources, carbon sources, metal ions and phosphate were examined for protease production of the isolate. Lactose and soytone peptone were the most effective carbon and nitrogen source for the enzyme production. After the late logarithmic growth phase the isolate began to produce the protease, and the maximum protease productivity was reached to 550 unit/ml in the optimized medium consisting of lactose (3%), soytone peptone (1.5%), $MgSO_4$ (0.1%), $K_2HPO_4$ (0.03%), and $KH_2PO_4$ (0.03%) at 28 h of incubation.

Effect of Microbubble Ozonation Process on Performance of Biological Reactor System for Excess Sludge Solubilisation (마이크로버블오존을 이용한 잉여슬러지 가용화 처리가 생물반응조의 성능에 미치는 영향)

  • Lee, Shun-Hwa;Jung, Kye-Ju;Kwon, Jin-Ha;Lee, Se-Han
    • Journal of Korean Society of Environmental Engineers
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    • v.33 no.2
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    • pp.113-119
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    • 2011
  • The study purpose was to examine an effect of zero emission of excess sludge on biological reactor and treated water quality within the biological reactor in the process of biological treatment combined with excess sludge reduction system with ozone. Under an ozone injection rate 0.03 g $O_3/g$ SS, Sludge Disintegration Number (SDN) 3 and less than pH 4 as pre-treatment process, it was possible to maintain a stable biological treatment process without sludge disintegration. In the test of $OUR_{max}$, of sludge, its value was hardly under the condition of ozone injection rate 0.03 g $O_3/g$ SS. There were almost no changes of MLVSS/MLSS within biological reactor followed by a solubilization of excess sludge. Accumulation of microorganism within biological reactor was also not observed. After solubilization of excess sludge, an increase for organic matter and SS concentrations of an effluent was not observed and T-N concentration was reduced by increasing nitrification and denitrification rate within biological reactor. Most of T-P was not removed by zero emission of excess sludge and was leaked by being included in effluents.

Effects of Coal Fly Ash on Composting Process of Household Garbage (음식쓰레기 퇴비화 과정에서 석탄회 첨가 효과)

  • Seong, Soon-Hee;Kim, Woo-Seong;Seo, Jeoung-Yoon
    • Korean Journal of Environmental Agriculture
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    • v.15 no.3
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    • pp.282-288
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    • 1996
  • Because of the high water content of the household garbage, it is difficult to compost it. Therefore, this study was conducted to investigate the possibility of using coal fly ash as humidity conditioner for the household garbage composting. The summarized results are as follows : 1. The maximum temperatures were $35^{\circ}C$ in spring, $47^{\circ}C$ in summer, and $43^{\circ}C$ in winter during the composting periods. 2. The mass was reduced to 70.5% after 60 days. The average volume reduced down to 74.7% after 60 days. 3. The seasonal variation of pH values showed a similar tendency and reached 8.5 after 60 days. 4. The water content was reduced at an early stage of composting and not much changed thereafter. It had 49.7% in spring, 33.9% in summer, and 56.5% in winter after 60 days. Ash contents were not much changed during the composting periods. 5. The contents of inorganic compounds were in the range of $0.06{\sim}4.03%$ $P_2O_5$, $0.01{\sim}2.87%$ CaO, $0.18{\sim}1.43%$ MgO, and $0.39{\sim}2.03%$ $K_2O$. Heavy metal contents were in the range of $ND{\sim}14.08$ Hg ${\mu}g/kg$, $ND{\sim}0.80$ Cd mg/kg, $4.99{\sim}28.95$ Cu mg/kg, $ND{\sim}242.62$ Cr mg/kg, $ND{\sim}20.24$ Pb mg/kg, and $ND{\sim}59.87$ Zn mg/kg.

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Determination of Rare Earth Elements by Inductively Coupled Plasma Mass Spectrometry with Introduction of Organic Phase Using Ultrasonic Nebulizer (초음파 분무기를 이용한 유기상의 주입에 의한 희토류 원소의 유도결합 플라스마 질량분석법적 정량)

  • Lee, Sang Hak;Son, Bum Mok
    • Analytical Science and Technology
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    • v.15 no.2
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    • pp.127-134
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    • 2002
  • Analytical method to determine rare earth elements which were extracted to organic phase by inductively coupled plasma mass spectrometry(ICP-MS) was investigated. Organic phase which had extracted rare earth elements was directly aspirated into ICP-MS by ultrasonic nebulizer(USN) in order to reduce solvent load to the plasma. Then, the count rate increased when MIBK(methyl isobutyl ketone) was added to EtOH(ethanol) but decreased when 0.03 M HEH(2-ethylhexyl-2-ethylhexyl phosphonic acid, $P_{507}$, PC88A) was added to solvent which mixed MIBK with EtOH. The optimal temperatures of desolvation system were -10 $^{\circ}C$ for the condenser and 150 $^{\circ}C$ for the heating tube. The optimal nebulizer flow rate which gave maximum count rate and minimum reflect power was 0.7 L/min. The optimal pH and extraction time were 4.3 and 10 min for MIBK-0.03 M HEH system. Detection limits which were obtained through calibration curves at the range of 0.2 ${\sim}$ 20 ng/mL were 0.02 ${\sim}$ 0.05 ng/mL under the optimal experimental conditions.

Characteristic of Damping Curve for the Directional System of Magnetic Compass (자기컴퍼스 방위지시부의 제진특성)

  • An, Yeong-Hwa;Jeong, Gong-Heun
    • Journal of the Korean Society of Fisheries and Ocean Technology
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    • v.21 no.1
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    • pp.28-34
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    • 1985
  • This paper describes that the characteristic of movement and the performance of the directional system of the liquid magnetic compass analyze and investigate in the kinds of compass and the coefficient of viscosity of the liquid of compass, which the horizontal magnetic field of the geo-magnetic is varied from the equator to the polar region (0.39 gauss-0.03 gauss) by use of apparatus generating artificial magnetic fields. The results are as follows; 1. It is confirmed that the measured values and the calculated values on the characteristic of damping curve by the type of compass and coefficient of viscosity has almost agree with one another. 2. As the horizontal magnetic force geo-magnetic field approaches around the equator (0.39 gauss), the horizontal magnetic field get near the polar region (0.03 gauss), its period is to be longer and the compass card prove not to be more stabilized. 3. The coefficient of viscosity of the liquid in the A, B, C, D and E compasses used in the experiment is estimated 0.03 poises, 0.02 poises, 0.02 poises, 0.015 poises and 0.048 poises respectively by computer simulation.

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Antimicrobial Activities and Nitrite-scavenging Ability of Olive Leaf Fractions (올리브 잎 분획물의 항균활성 및 아질산염 소거능)

  • 이옥환;이희봉;손종연
    • Korean journal of food and cookery science
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    • v.20 no.2
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    • pp.204-210
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    • 2004
  • This study was performed to provide basic physiological activities data to predict the usefulness of olive leaves as a food material. Total flavonoid and total phenol contents of 80% ethanol extract of olive leaf were 5.81% and 14.8%, respectively. Total flavonoid and total phenol contents were markedly higher in butanol and ethyl acetate fractions than in hexane, chloroform, and water fractions (p<0.05). Oleuropein in olive leaf was the major phenolic compound. The oleuropein contents of 80% ethanol extract, butanol and ethyl acetate fractions of olive leaf were 102.11${\pm}$0.02, 173.35${\pm}$0.03 and 152.71${\pm}$0.03 mg/100g, respectively. The 80% ethanol extract, butanol and ethyl acetate fractions of olive leaf showed a growth inhibitory effect to Bacillus cereus, Staphylococcus aureus, Escherichia coli, and Salmonella enteritidis, whereas antimicrobial activities of hexane and chloroform fractions were not observed. The inhibitory activity to ACE was determined to be very weekly positive in 80% ethanol extract and all fractions of olive leaf. The nitrite-scavenging ability of 80% ethanol extract, butanol and ethyl acetate fractions of olive leaf were 72.8%, 76.0% and 75.4%, respectively. Significant evidence was detected that the butanol and ethyl acetate fractions showed higher activity than that of hexane, chloroform, and water fractions (p<0.05).

First Principles Calculations on Electronic Structure and Magnetism of Transition Metal Doped ZnO (전이금속이 도핑된 ZnO의 전자구조와 자성에 대한 제일원리계산)

  • Yun, Sun-Young;Cha, Gi-Beom;Hong, Sun-C.
    • Journal of the Korean Magnetics Society
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    • v.15 no.1
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    • pp.1-6
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    • 2005
  • In this study we investigate the electronic structure and magnetism of transition metal (TM = Ti, Cr, Mn, Fe, Co, Ni, Ru, Pd, Ag ) deped ZnO($TM_{0.25}Zn_{0.75}O$), which are expected to have Curie temperature. Full-potential Linearized Augmented Plane Wave(FLAPW) metod is adopted with exchange-correlation potential expressed as general gradient approximation(GGA). The calculated magnetic moments of ($TM_{0.25}Zn_{0.75}O$) are 0.83, 3.03, 4.03, 3.48, 2.47, 1.56, 0.43, 0.75, 0.01 ${\mu}_B$ for TM = Ti, Cr, Mn, Fe, Co, Ni, Ru, Pd, Ag, respectively. The nearest neighbor O atom to the transition metal is calculated to have a significant magnetic moment of about 0.1${\mu}_B$, ?? 새 strong hybridization between O-p and TM-d bands. As the results, the systems may have larger magnetic moments in total, compared to the corresponding isolated atoms. The 3d TM doped systems exhibit the half-metallic character except Co, wheres the 4d TM doped systems behave like normal metals and low spin polarization at the Fermi levels.

Selection of Lactic Acid Bacteria Specifically Inhibiting the Growth of Helicobacter pylori (Helicobacter pylori의 생육을 특이적으로 억제하는 유산균 선발)

  • 정후길;김응률;전석락
    • Korean Journal of Microbiology
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    • v.37 no.2
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    • pp.151-157
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    • 2001
  • This study was conducted to select lactic acid bacteria which possess potential inhibitory effect on Helicobacter pylori, and to make feasibility test of fermented milk products using them. In order to select lactic acid bacteria specifically inhibiting the growth of H. pylori, antibacterial activity using paper disk method, adherence ability to Caco-2 cell inhibitory effect on urease activity of H. pylori, and milk fermentation feasibility were measured. Among 45 strains of lactic acid bacteria tested, 28 strains showed clear zone and Lactobacillus gasseri MK-03 showed the largest clear zone. Caco-2 cell adherence by lactic acid bacteria and inhibitory effect of them on H. pylori adherence were also evaluated. Of 28 strains tested, 18 strains appeared to be effective on adherence to Caco-2 cell, and especially Bifidobacterium longum MK-26 was found to be superior to others. When Bif. longum MK-26 and H. pylori were reacted with Caco-2 cell 2hrs before, adherence percentage of H. pylori decreased from 0.105% to 0.004%. To investigate inhibitory effect of lactic acid bacteria-derived supernatant on urease activity of H. pylori, pH-adjusted fermented supernatant(pH-4.4) was assessed by co-cultivation method. There of Lb. acidophilus MK-07-derived supernatant showed the most inhibitory effect on urease activity of H. pylori. Considering milk fermentation ability of selected 3 strains, they were comparably feasible to fermented milk products. Consequently, Lb. gasseri MK-03, Lb. acidophilus MK-07, and Bif. longum MK-26 were selected to specifically inhibit the growth of H. pylori, by antibacterial activity, inhibition of urease activity, and inhibition of Caco-2 cell adherence, respectively.

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EFFECT OF RESIN MONOMERS ON THE ACTIVITY OF CARIOGENIC BACTERIA (수종의 레진 모노머가 우식 유발성 세균의 활성에 미치는 영향)

  • Seo, Young-Ju;Kook, Joong-Ki;Yoon, Jung-Hoon;Kim, Su-Gwan;Lee, Nan-Young;Lee, Sang-Ho
    • Journal of the korean academy of Pediatric Dentistry
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    • v.31 no.2
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    • pp.247-255
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    • 2004
  • The purpose of this study was to examine the effects of resin composite monomers (Bis-GMA, TEGDMA, EGDMA, UDMA, HEMA, Camphorquinone) on the growth of the two cariogenic bacteria, Streptococcus mutans and Streptococcus sobrinus. We obtained the following results : 1. The growth rate of S. mutans and S. sobrinus was decreased significantly in the group of all composite resin monomer at a concentration of 0.03mmo1/L(P<0.01). 2. The growth rate of S. mutars in the group of UDMA at a concentration of 0.01 mmol/L and the group of CQ at a concentration of 0.005 mmol/L, 0.01 mmol/L was decreased significantly compared to the control group(P<0.01). 3. The growth rate of S. sobrinus in the group of HEMA, UDMA at a concentration of 0.01 mmol/L and the group of CQ at a concentration of 0.005mmol/L. 0.01mmo1/L was decreased significantly compared to the control group (P<0.01). 4. The growth rate of S. sobrinus in the group of EGDMA at a concentration of 0.001, 0.01, 0.03mmo1/L was decreased significantly compared to the control group(P<0.01) and were showed to be statistically significant difference between experimental groups(P<0.01).

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