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Analysis of Genetic Diversity of Korean Accessions of the Genus Acorus Using RAPD Markers and NIR Spectroscopy

  • Lee, Ja-Hyun;Kim, In-Seon;Lee, Seong-Gene;Rim, Kwang-Sub;Kim, Sung-Gil;Han, Tae-Ho
    • Horticultural Science & Technology
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    • v.29 no.3
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    • pp.232-239
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    • 2011
  • The genus Acorus is known as an indigenous medicinal plant. Genetic diversity of thirteen accessions of A. calamus and eight of A. gramineus, with an accession of Colocasia antiquorum and two of Iris pseudacorus as outgroups, were evaluated using RAPD markers for cluster analysis and principal coordinate analysis, and NIR spectroscopic profiles for principal component analysis.A total of 371 polymorphic bands were obtained by using the selected 12 random primers. The genetic distances were estimated from 0.03 to 0.31 within A. calamus and from 0.03 to 0.51 within A. gramineus. The dendrogram and three-dimensional plot separated the accessions into four distinct groups (A. calamus, A. gramineus, C. antiquorum, and I. pseudacorus). Moreover, for the diversity among genus Acorus, eleven A. calamus accessions, one A. gramineus accession, and two I. pseudacorus accessions were non-destructively analyzed from their leaves by NIR spectroscopy, which discriminated Acorus accessions like the RAPD analysis. Interestingly, thirteen accessions of A. calamus were clustered into two groups based on RAPD and NIR analyses, which indicates that there are two ecotypes of A. calamus in Korea. An accession (CZ) of A. calamus with yellow stripe on leaves was closely grouped with another (CX) at a genetic distance (GD) of 0.03, which shows that the stripe trait might be generated by chimeric mutation. The genetic distance between A. calamus and A. gramineus was revealed to be farthest from 0.80 to 0.88 GD. In genus Acorus the genetic diversity and genetic variation were identified by using RAPD marker technique and non-destructive NIRs.

Influence of gas mixture ratio on the secondary electron emission coefficient (${\gamma}$) of MgO single crystals and MgO protective layer in surface discharge AC-PDPs

  • Lim, J.Y.;Jung, J.M.;Kim, Y.G.;Ahn, J.C.;Cho, T.S.;Cho, D.S.;Kim, J.G.;Kim, T.Y.;Kim, S.S.;Jung, M.W.;Choi, S.H.;Ko, J.J.;Kim, D.I.;Lee, C.W.;Seo, Y.;Cho, G.S.;Kang, S.O.;Choi, E.H.
    • 한국정보디스플레이학회:학술대회논문집
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    • 2000.01a
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    • pp.117-118
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    • 2000
  • The secondary electron emission coefficient ${\gamma}$ of MgO single crystals according to the gas mixture ratio of Xe to Ne have been investigated by ${\gamma}-focused$ ion beam system. It is found that the MgO single crystals of (111) crystallinity has the highest ${\gamma}$ from 0.09(0.03) up to 0.16(0.04), while from 0.07(0.02) to 0.15(0.03) for (200) and from 0.06(0.01) to 0.13(0.02) for (220) crystallinity for operating Ne (Xe) ions ranging from 50eV to 200eV throughout this experiment. And it is found that the ${\gamma}$ ranged from 0.03 up to 0.06 for Ne-Xe mixtures are much smaller than those of 0.09 up to 0.16 for pure Ne ions under accelerating voltage ranged from 50V to 200V.

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Piezoelectric and Dielectric Properties on PSN-PMN-PZT Composition according to CeO2 Addition (PSN-PMN-PZT 조성의 CeO2첨가에 따른 압전.유전특성 변화)

  • Yoon, Man-Soon;Chio, Yong-Gil;Ur, Soon-Chul
    • Journal of the Korean Institute of Electrical and Electronic Material Engineers
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    • v.19 no.9
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    • pp.838-842
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    • 2006
  • 0.03Pb$(Sb_{0.5}Nb_{0.5})O_{3}-0.03Pb(Mn{1/3}Nb{2/3)O_{3}-(0.94-x)PbTiO_{3}-xPbZrO_{3}$ ceramics doped with $CeO_{2}$ were synthesized by conventional bulk ceramic processing technique. Phases analysis, microstructures and piezoelectric properties were investigated as a function of $CeO_{2}$ content (0.03, 0.05, 0.1 0.3, 0.5 and 0.7 wt%). Microstructures and phases information were characterized using a scanning electron microscope (SEM) and an X-ray diffractometer (XRD). Mechanical quality factor ($Q_{m}$) and coupling factor(kp) were obtained from the resonance measurement method. Both $Q_{m}$ and $k_{p}$ were shown to reach to the maximum at 0.1 wt% $CeO_{2}$. In order to evaluate the stability of resonance frequency and effective electromechanical coupling factor ($K_{eff}$) as a function of $CeO_{2}$, the variation of resonance and anti-resonance frequency were also measured using a high voltage frequency response analyzer under various alternating electric fields from 10 V/mm to 80 V/mm. It was shown that the stability of resonance frequency and effective electromechanical coupling factor were increased with increasing the $CeO_{2}$ contents.

A Study on Concentration of Bleomycin to Induce Lung Fibrosis in Obese Animal Model (비만 생쥐모델의 폐약(肺弱)을 유발하기 위한 Bleomycin의 농도 결정 연구)

  • Koh, Young-Mee;Jang, Soon-Woo;Ahn, Taek-Won
    • Journal of Haehwa Medicine
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    • v.29 no.1
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    • pp.1-17
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    • 2020
  • Objectives : The objectives of this study is to develop a mouse model similar to Taeeum-type by inducing Lung fibrosis with bleomycin, and to determine adequate concentration of bleomycin. Methods : The subjects were divided into six groups: normal, obesity induced group, and bleomycin administered 0.015U, 0.03U, 0.06U, and 0.09U(U/100g bw) concentrations respectively. Each concentration of bleomycin was dissolved in distilled water, and administered through Intra-Nazal-Trachea injection method. Food intake and body weight were measured at regular time weekly. At the end of the experiment, blood was gathered by cardiac puncture for biochemical examinations, organs were removed for histological examinations, and weigh and mRNA genes was analyzed. Result : Mice administered with bleomycin at 0.015U and 0.03U showed body and fat weight gain, and increased blood total cholesterol, LDL-cholesterol, glucose, and free fatty acid level. Fat related genes also showed higher level than the control group. Obesity was most strongly induced in the mice administered with 0.03U of bleomycin. On the other hand, when bleomycin was administered at concentrations above 0.06U, a model of obesity mouse was not created due to rapid emphysema inflammation and weakness. Conclusions : Mice were most vulnerable to obesity when bleomycin was administered at a concentration of 0.3 to cause liver damage. Bleomycin concentration over 0.06U did not cause obesity-induced mice, due to severe damage in liver.

Photodissociation of C3H5Br and C4H7Br at 234 nm

  • Kim, Hyun-Kook;Paul, Dababrata;Hong, Ki-Ryong;Cho, Ha-Na;Lee, Kyoung-Seok;Kim, Tae-Kyu
    • Bulletin of the Korean Chemical Society
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    • v.33 no.1
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    • pp.143-148
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    • 2012
  • The photodissociation dynamics of cyclopropyl bromide ($C_3H_5Br$) and cyclobutyl bromide ($C_4H_7Br$) at 234 nm was investigated. A two-dimensional photofragment ion-imaging technique coupled with a [2+1] resonanceenhanced multiphoton ionization scheme was utilized to obtain speed and angular distributions of the nascent $Br(^2P_{3/2})$ and $Br^*(^2P_{1/2})$ atoms. The recoil anisotropies for the Br and $Br^*$ channels were measured to be ${\beta}_{Br}=0.92{\pm}0.03$ and ${\beta}_{Br^*}=1.52{\pm}0.04$ for $C_3H_5Br$ and ${\beta}_{Br}=1.10{\pm}0.03$ and ${\beta}_{Br^*}=1.49{\pm}0.05$ for $C_4H_7Br$. The relative quantum yield for Br was found to be ${\Phi}_{Br}=0.13{\pm}0.03$ and for $C_3H_5Br$ and $C_4H_7Br$, respectively. The soft radical limit of the impulsive model adequately modeled the related energy partitioning. The nonadiabatic transition probability from the 3A' and 4A' potential energy surfaces was estimated and discussed.

An Analysis on Kinematically Contributing Factors at Impact of Forehand Drive Motion in Squash (스쿼시 포핸드 드라이브 동작의 임팩트시 운동학적 주요요인 분석)

  • Lee, Kyung-Il;Lee, Hee-Kyung
    • Korean Journal of Applied Biomechanics
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    • v.17 no.1
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    • pp.29-39
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    • 2007
  • This study were obtained elapsed time phase-by-phases, displacement, user angle, velocity and angular velocity to analyse kinematically contributing factors at impact of forehand drive motion, on targeting three male players. The results of the study were presented as follows; In the forehand drive swing, the elapsed time by phases was a total of .52 seconds: .30 seconds from backswing to impact and .22 seconds from impact to follow-through, Considering the mean change in locations of COM of each(part$\rightarrow$body segment) at impact, racket head, left shoulder, right wrist and left hip, the left-right directions(X-axis) were showm to be each $.61{\pm}.03$, $1.19{\pm}.08$, $.66{\pm}.03$, $.94{\pm}.06$, and $.45{\pm}.03m$. The displacement differences of COM of each body segment were shown to be -.57, -.05, -.33, and .16m. For the vertical direction(Z-axis), the center of mass was lowest at impact and highest at E3. For the displacement of the right wrist on the left hip, the right wrist moved to .82m to the lower direction without change in the locations of the hip from E1 from E2. When the left hip moved .02m from E2 to E3, the right wrist moved .7m in the upper direction. In respect to the velocity of each body segment, the hip and the shoulder joint accelerated and then the wrist followed. Then the right wrists of all the subjects and their racket heads showed maximum speed, and an effective swing was observed. At the angle of each part, the angle of the right wrist was the smallest at the backswing and the largest at the moment of the impact. Then it increased gradually in the follow-through section. In respect of angular velocity for subject A, the hip moved and the largest change occurred. Immediately before the impact, the subject made a swing using his right wrist, his hip, and the shoulder joint, showing the maximum value, which was judged to be effective.

Studies on the volatile aroma components of Edible mushroom (Tricholoma matsutake) of Korea (한국산(韓國産) 식용(食用)버섯의 향기성분(香氣成分)에 관(關)한 연구(硏究) (I) -송이 버섯의 향기성분(香氣成分)-)

  • Ahn, Jang-Soo;Lee, Kyu-Han
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.15 no.3
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    • pp.253-257
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    • 1986
  • The aroma component analysis of raw and cooked Korean edible mushroom (Trichloma matsutake) by GC, GC-MS is as follows; 1) The volatile aroma component of raw mushroom is identified as 13 kinds, and among them, 4 kinds of aroma component such as 1-octene-3-ol (73.95%), methyl cinnamate (12.52%), 2-octanol (7.62%) and octyl alcohol (2.78%)-consists 95.87% of total aroma component 2) Meanwhile, The volatilearoma component of cooked one is identified as 9 kinds and 4 of them-1-octen-3-ol (64.94%), methyl cinnamate (22.03%), 2-octanol (7.68%), and octyl alcohol (3.31%)-consists 89.61% of total aroma component. 3) The major composition of aroma component of both raw cooked ones are carbonyl compounds and alcohols. Their number of carbons is $C_8$ short chain aliphatic compounds.

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Maternal killer-cell immunoglobulin-like receptors and paternal human leukocyte antigen ligands in recurrent pregnancy loss cases in Turkey

  • Elbasi, Mehmet Onur;Tulunay, Aysin;Karagozoglu, Hale;Kahraman, Semra;Eksioglu-Demiralp, Emel
    • Clinical and Experimental Reproductive Medicine
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    • v.47 no.2
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    • pp.122-129
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    • 2020
  • Objective: The survival of a semi-allogeneic fetus depends on several immunological mechanisms, and it has been suggested that recurrent pregnancy loss (RPL) could develop as a result of one or more immunological abnormalities. Methods: Compatibility between partners for human leukocyte antigen (HLA) genotypes and the relationships between maternal killer-cell immunoglobulin-like receptor (KIR) and paternal HLA-Bw4/Bw6 and HLA-C1/C2 supra-groups were investigated in 25 couples with RPL in comparison to healthy couples with children. HLA and KIR genotyping was performed using polymerase chain reaction with sequence-specific primers and/or sequence-specific oligonucleotides. Results: HLA class I incompatibility between partners, especially in HLA-B alleles, was more common in the RPL group (p= 0.01). HLA-C2 homozygosity was more frequent in the male partners of RPL couples than in other groups (p= 0.03). The KIR2DL5 gene frequency was significantly higher in both the female and male partners of RPL couples, whereas the KIR2DS3 gene frequency in male partners of RPL couples was significantly reduced (p= 0.03). The presence of KIR2DL3 in women with RPL was correlated with the presence of HLA-C2 alleles in their spouses (p= 0.03). Conclusion: Our data from a Turkish population suggest that male HLA-C2 homozygosity may play an important role in RPL. Additionally, an incidental match between male HLA-C2 and female HLA-C1 ligand KIR receptors might perturb the balance between activatory and inhibitory KIR-ligand interactions during pregnancy in couples affected by RPL. The roles of orphan KIR2DL5 and orphan KIR2DS3 in RPL remain obscure.

Genetic Differences and Variation in Two Purple Washington Clam (Saxidomus purpuratus) Populations from South and North Korea

  • Yoon, Jong-Man;Park, Su-Young
    • The Korean Journal of Malacology
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    • v.22 no.2
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    • pp.97-108
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    • 2006
  • Genomic DNA samples isolated from geographical purple Washington clam (Saxidomus purpuratus) were obtained from two different regions in Korean Peninsula: Gunsan (Gunsan population; GSP), and Haeju (Haeju population; HJP), a collection area in the vicinity of the West Sea. The seven arbitrarily primers, OPA-07, OPA-09, OPA-18, OPA-20, OPC-03, OPC-06 and OPC-09 were shown to generate the total loci, loci observed per primer, shared loci by each population, specific, and polymorphic loci which could be clearly scored. We also generated the unique shared loci to each population and shared loci by the two populations in purple Washington clam. The size of the DNA fragments also varied wildly, from 50 to 2,400 bp. Here, 304 total loci were identified in the GSP purple Washington clam population, and 282 in the HJP: 91 polymorphic loci (29.9%) in the GSP and 47 (16.7) in the HJP. 198 shared loci, with an average of 28.3 per primer, were observed in the GSP population. The decamer primer OPA-07 generated the shared loci by the two populations, approximately 1,000 bp, between the two Saxidomus populations. The oligonucleotide primer OPC-03 also generated the shared loci by the two populations, approximately 500 bp and 1,000 bp, in GSP population from Gunsan and HJP population from Haeju. The other primer, OPC-06 generated the shared loci by two Gomphina populations (approximately 400 bp). The dendrogram, generated by seven reliable primers, indicates three genetic clusters. The dendrogram obtained by the seven primers indicates three genetic clusters: cluster 1 (GUNSAN 01-GUNSAN 02), cluster 2 (GUNSAN 03-GUNSAN 11), and cluster 3 (HAEJU 12-HAEJU 22). The genetic distance between the two geographical populations ranged from 0.043 to 0.506. Especially, the longest genetic distance displaying significant molecular differences, 0.506, was found to exist between individuals GUNSAN no. 11 of Gunsan and HAEJU no. 17 of Haeju.

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A Study on Solution Blend of Poly(vinyl chloride) with Poly(VC-co-AN) (폴리염화비닐(PVC)/염화비닐-아크릴로니트릴 공중합체의 용액 블렌드에 관한 연구)

  • Lee, Soo;Oh, Gang-Seok;Kim, Seung-Ryung;Oh, Young-Se
    • Polymer(Korea)
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    • v.26 no.5
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    • pp.559-567
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    • 2002
  • Poly (vinyl chloride) was blended with blended with vinyl chloride-acrylonitrile copolymers (MO-01 : M$\sub$w/=199049, AN=22.79%, MO-02 : M$\sub$w/=238523, AN=47.03%, MO-03 : M$\sub$w/=289496, AN=52.49%, MO-04 : M$\sub$w/=341837, AN=58.68% by mixing dimethylformamide (DMF) solution of each polymer and their morphology, viscosity, viscoelastic and mechanical properties were studied. Blends of PVC and MO-01 showed a homogeneous morphology. On the contrary, the other copolymer blends showed a large phase separation with spherical domains. Miscibility of blends of PVC and MO-01 was shown to be better than that of blends of other copolymers from morphological and viscoelastic studies. Even though blends of PVC and MO-01 were observed to be the considerable compatibility at a composition of PVC/MO-01=90/10, the mechanical properties of blend films were not improved too much in most blend cases.