• Journal of Embryo Transfer
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• v.16 no.1
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• pp.35-40
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• 2001

Artificial insemination (AI) with frozen or cooled semen is widely used in commercial fields of cattle and pig. Little is known about characteristics of canine sperm after freezing or cooling. For both practical and commercial goal, the canine semen treated with cooling and freezing should be carried out to exam the fundamentals, including sperm motility, survivability and fertilizing capacity. The aim of this study, thus, was to identify the effects of extended exposure to 4$0^{\circ}C$ on canine semen by motility, survivability, acrosomal changes following different duration. Fifteen ejaculates collected by digital manipulation twice per week from 3 dogs (Shih-Tzu) were divided to 16 aliquots after adding Tris-egg yolk (TE) buffer formulated by our laboratory, and cooled from 37 to 4$^{\circ}C$, by ramp rate of 0.6$^{\circ}C$/min. Each sample was evaluated by their motility, survivability and the acrosomal status at 0h (control), 2h, 12h and 1 d~10 d, respectively. The motility of spermatozoa was graded to 6 levels using the modified method of Seager. The survivability of sperm was assessed using an epifluorescence microscope after Fert/Light (Mole-cular Probes Inc.) staining. To estimate the proportion of the spermatozoa of intact acrosome, 200 spermatozoa were assessed in randomly selected fields, using epifluorescence microscope after FITC/PSA (Sigma) staining. At 2 h after cooling, the motility of most spermatozoa were assessed to be grade 0 and 1. At 12 h, high number of sperm were in grade 0 to 1, however, it was significantly (P<0.05) lower than that of 2 h. From 1 d to 4 d, ~50% of sperm was assessed to grade 0 to 1. On day 7, a little sperm were in grade 0 to 1. No sperm showed motility on day 10. Sperm motility was rapidly reduced by the percent of 10% of grade 0 to 1. From 2 h to 6 h, the number of live sperm was 90% and the sperm chilled for 10 days lived>50%. Acrosomal intact of spermatozoa exposed to 4$^{\circ}C$ for 2 h was 51%, supposed the sperm of control was 100%. Our results suggest that 1) this is easy to transfer and preservation for short periods 2) AI can be used by semen chilled for 6-Day.

• Journal of Physiology & Pathology in Korean Medicine
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• v.19 no.5
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• pp.1296-1302
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• 2005

Laminariae Thallus has been known to be effective for the treatment of obesity. Te show the effectiveness of Laminariae Thallus in a more scientific way, Laminariae Thallus extract was prepared and evaluated in high fat diet rats by measuring the changes of body weight and lipid metabolism as described briefly below. 200 g of crushed Laminariae Thallus was extracted with methyl alcohol. The extract was evaporated under reduced pressure to give 14.4g. For 10 weeks, control group rat were ingested high fat diet, while the test group rat were fed with a fat diet pius Laminariae Thallus extract. Normal group was fed with normal diet. 150 mg of Laminariae Thallus extract per 1 kg of body weight was added to the diet in a test group rats. Control group rats on a high fat diet gained weight significantly, whereas the test group rats on a high fat diet plus Laminariae Thallus extract gained weight less. Significant increase of liver weight caused in a high fat diet was also inhibited by the Laminariae Thallus extract treatment. Total lipid, triglyceride, phospholipid and total cholesterol levels of serum in the high fat diet rats were remarkably increased, whereas their levels on a high fat diet plus Laminariae Thallus extract were less increased. While serum HDL-cholesterol levels was remarkably decreased in a high fat diet, its level was less decreased in a high fat diet plus Laminariae Thallus extract. Furthermore, we observed that the activities of hepatic acetyl-CoA carboxylase and fatty acid synthetase increased in a high fat diet, while their activities in a high fat diet plus Laminariae Thallus extract were getting back nearly to the normal levels of normal diet rats. These result showed that the obesity caused by a high fat diet was effectively inhibited by a Laminariae Thallus extract. Our results also showed that the abnormal lipid metabolism caused by a high fat diet was effectively cured by adding Laminariae Thallus extract.

• Food Science of Animal Resources
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• v.28 no.2
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• pp.187-195
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• 2008

The purpose of this study was to establish proper shipping weight and backfat thickness by applying the growth model to backfat thickness, measured by means of not only body weight, but also ultrasonography, and predicting the changes by age. Three breeds, i.e. Duroc, Landrace, and Yorkshie, were analyzed, and the Gompertz, logistic, and Von Bertalanffy model were used for inference with the parameter of the growth model being sex. As a result, both body weight and backfat thickness showed different growth curve parameters and characteristics at inflection points depending on model selection and sex. As for backfat thickness, in estimating the inflection point, unlike the case of body weight, the inflection ages of the boars of the Duroc breed was earlier than that of sows, whereas the inflection ages of the sows of the Landrace and Yorkshire breeds was earlier than that of boars. More than anything else, in the analysis of the changes in backfat thickness according to body weight, as the body weight reached 145kg, the backfat thickness showed much variation as great as 1.7-3.2 cm in each breed and sex. In addition, unlike the other breeds, the boars of the Landrace breed showed an exponential type of relationship between body weight and backfat thickness. As they grow to become 100 kg or heavier, abrupt change in back fat thickness was confirmed. If the growth of body weight and backfat thickness is understood and the genetic relationship is taken advantage of like this, it would be possible to set desired body weight and backfat thickness, and thus help effectively set the shipping time. If not only the phenotype, but also genetic parameters about growth characteristics are estimated and analyzed additionally, more effective data can be generated.

• Korean Journal of Medicinal Crop Science
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• v.26 no.3
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• pp.227-232
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• 2018

Background: Angelica gigas Nakai is a perennial herb belonging to the family Umbelliferae. Its roots are utilized in traditional medicine. The aim of this study was to increase the yield of and the content of two indicator components (decursin and decursinol angelate) in A. gigas Nakai. Methods and Results: The roots of A. gigas Nakai were harvested 4-times from late August to late October in 2017. Two agents (trace element-TE, and plant hormone-HM) were applied 4 times at intervals of 2 weeks. The content of the two indicator components were analyzed by high performance liquid chromatography. The HM treatment showed the greatest increase in underground part yield and root diameter. The content of the two indicator components in the control (non-treatment) group was the highest in the underground part, but was higher in the aerial parts in the agent treatment group. After treatment with the agents, the content of the indicator components tended to decrease in the underground part. However, the total content of the indicator components in the two agent treatment groups exceeded the level of 6% set by the Korean Pharmacopoeia. Conclusions: The highest underground part yield was found in the HM treatment group, while the highest content of decursin and decursinol angelate were found in the control group. This study provides basic information for yield improvement in A. gigas.

• Korean Journal of Microbiology
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• v.37 no.2
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• pp.170-175
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• 2001

In order to produce xylitol with high yield, experiments were carried out to develope xylitol dehydrogenase(XDH) defective mutant from P. stipitis and to investigate the xylitol fermentation characteristics of mutant strain. After treatment of P. stipitis with EMS, mutant PXM-4 was selected based on te XDH activity and xylitol production capability. Among the tested cosubstrates, galactose was selected as an adequate cosub-strate on xylitol production of mutant PXM-4. But with the increase in the concentration of galactose in the medium, xylitol production was decreased because the transport of xylose into cell was inhibited by galactose. The optimal concentration of galactose for the production of xylitol using 20 g/ι xylose was 20 g/ι Under this condition, maximum concentration of xylitol and yield were 14.4 g/ι and 97%, respectively. In order to prevent the inhibitory effect of xylose transport by galactose, galactose was fed with low concentration and the concentration of xylitol produced was increased up to 25 g/ι.

• Korean Journal of Microbiology
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• v.35 no.2
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• pp.158-163
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• 1999

A ploygalacturonase-produchg yeast was isolated from Cheju soil by selective eivichment media. One strain which has the highesl activity of polygalacturonase was selected. The characle~ishcs of the strain CS-2 were as follows: CS-2 utilized xylose. sucrose, maltose, u.ehalose, cellobiose. melibiose, lactose, raffinose, inosiiol, dulicilol, and dextrose, but did not utilized galactose, nitrate. nit~te, and lysine. Growth of CS-2 was inhibited by cyclohexamide, 1% acetic acid, and high concenaation (over 50%) of glucose. It grew at $30^{\circ}C$ but did 'IIOL $35^{\circ}C$. The cell size ofthe strain CS-2 was 2.9 p ~ n in length and 1.3 $\mu$ in diameter. Vegetable reproductmn was multiple budding and ascospre was present I to 4. Pseudomycelia or true myceliua formation were not observed In any of the cullureq. These results suggest that strain CS-2 is most likely a strain related Cryptococcus spp. (Cryptococcu spp. CS-2). When polygalacturonase or ihe yeast was induced by addition of polygalactoronic acid, polygalacturonase activity was detected in culture supernatent. There was a peak of specific activity a1 he mid-stationary phase(3 days culture) of growth. Polygalacturonase specific activity of Crylmcoccus sp. CS-2 was 2.96 unitsling. The molecular weighl ol'polygalacturonase was showed to be 46 KDa by both SDS-PAGE and activity stailling.

• Korean Journal of Materials Research
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• v.4 no.3
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• pp.268-279
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• 1994

The x-ray powtler pattern of single phase $Bi_2S_2CaCu_2O_{8+x}$ has been identified and fullyindexed using a pseudotetragonal subcell with a= 5.408, c = 30.83 $\AA$ and an incommensurate supercellwith reciprocal lattice vector, X$q^*$, given by $q^*=0.211b^*-c^*$. The x -ray powder pattern of the Pb-free110K superconductor phase "$Bi_2S_2CaCu_2O_{10+x}$" has many lines which belong t.o an incommensuratesupercell. Using elect.ron d~ffraction pImt.ographs as a indexing guide, an indexing scheme for the powderpattern has been obtained. The unit cell has a geometrically orthorhombic subcell a=5.411, b= 5.420, c=37.29(2) $\AA$. Supercell reflections have indices that are derived from the subcell k, 1 indices by addition uf$\pm q^*$, where $\pm q^*=0.211b^*-0.78c^*$The incommensurate con~ponent In the b dwection, $\delta$, is the same for both phases but on going from2212 to 2223 phase, the superlattic component in the c direction changes from commensurate($\varepsilon$=1) toincommensurate($\varepsilon$=0.78).X>$\varepsilon$=0.78).

• Journal of the korean Society of Automotive Engineers
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• v.18 no.5
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• pp.53-68
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• 1996

마그네슘이 자동차 경량화에 관심이 되는 이유는 근본적으로 CAFE 규제와 같이 경량화를 통한 화석연료의 소모를 크게 억제해야 한다는 사회적인 규제이나, 지난 10여년간의 기술발전으로 내식성이 나쁘다거나 취급이 위험한 금속이라는 인식이 크게 개선된 데에도 있다. 다른 경량금속에 대한 Mg 지금 가격의 비교조건이 호전되었고 향후 원소 재공급의 다변화가 추진되고 있는 것도 환경을 변화시킨 중요한 요인이다. 그간 중요한 경량화 대체 재료로 연구투자가 많았던 유기고분자 재료 및 FRP 등과 같은 복합재료는 폐기부품의 재활용이 어려움 때문에 호나경친화적인 단점이 부각되어, 이 소재의 증가가 주춤해 있다. 마그네슘의 경우에는 재활용이 가능하고, 진동흡수효과가 매우 커서 소음발생을 크게 줄일 뿐만 아니라, 주행 및 내구성시험에서 치수안정성이 좋고 많은 종류의 전자기기 사용에 의한 전자파 차폐효과도 큰 장점을 가지고 있다. 본 고에서는 Mg 다이캐스팅으로 자동차부품의 경량화 현황과 선진국에서 보는 전망을 미국을 중심으로 정리하고, 이와 관련한 Mg 다이캐스팅으로 자동차부품의 경량화 현황과 선진국에서 보는 전망을 미국을 중심으로 정리하고, 이와 관련한 Mg 기술적인 이슈와 시장전망도 서술하였다. 그리고 현재 우리나라의 연구계와 부품업계에서 추진하고 있는 연구개발 동향을 자동차 업계에 소개하는 의미도 있다. 이처럼 우리나라의 현황을 정리해 보는 것은 국내 자동차 산업이 국제적인 경쟁을 하고 있고 Mg기술과 원료확보에서 일본의 견제를 받고 있는 우리의 현실에서도 필요한 작업으로 생각된다.값들로 구성되는 형상을 내구 성능, 성형성등을 고려하여 최종 형상으로 결정한다. 내구성능의 예측은 금속부품의 내구수명 예측에 널리 이용되고 있는 방법이 방진 고무부품의 경우에도 적용 가능한지를 검토하고, 방진 고무부품에도 일반적으로 적용될수 있는 내구수명 예측방안의 개발 가능성을 타진해 보았다. 본 연구의 목표는 시제품을 제작하기 이전에 설계된 부품에 대한 스프링 상수 및 내구특성을 체계적으로 규명하여 제품 시험의 횟수를 줄이고, 보다 정밀한 제품을 제작할 수 있도록 하기 위한 것이다.세포수는 초기 배반포기배에서 팽윤 배반포기배로 진행됨에 따라 두배에서 세배 정도 증가되었음을 알 수 있었다. 또한, differential labelling과 bisbenzimide기법에서 얻어진 각각의 총세포수를 비교하였을 때 총세포수는 발달의 진행 정도에 따라 증가되며 그와 동시에 동일한 군 간의 세포수도 거의 유사함을 알 수 있었다. 따라서, ICM과 TE를 differential labelling하는 기법은 수정란의 quality를 평가하는데 매우 유용한 기법으로서 착상전 embryo 발달을 연구하는데 효과적으로 이용될 수 있다는 것을 시사한다. 고도의 유의차를 나타낸 반면 비수구, 초생수로구 및 Bromegrass 목초구 간에는 아무런 유의차가 인정되지 않았다. 7. 농지보전 처리구인 배수구와 초생수로구는 비처리구에 비해 낮은 침두 유출량과 낮은 토양유실량을 나타내었다.구보다 14% 절감되는 것으로 나타났다.작용하는 것으로 사료된다.된다.정량 분석한 결과이다. 시편의 조성은 33.6 at%

• Reproductive and Developmental Biology
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• v.31 no.3
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• pp.153-159
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• 2007

In this study, using FIV-based lentivirus vector system, we tried to express hG-CSF in tetracycline-controllable manner. hG-CSF influences the proliferation, differentiation, and survival of cells in the neutrophil lineage. To enhance stability and translation of hG-CSF transcript, WPRE sequence was also introduced into FIV-Tet-On vector at downstream region of either the hG-CSF gene or the sequence encoding rtTA. Primary culture cells (CEF, chicken embryonic fibroblast; PFF, procine fetal fibroblast) infected with the recombinant FIV were cultured in the medium supplemented with or without doxycycline for 48 hours, and induction efficiency was measured by comparing the hG-CSF gene expression level using quantitative real-time PCR, Western blot and ELISA. Higher hG-CSF expression and tighter expression control were observed from the vector in which the WPRE sequence was placed at downstream of the hG-CSF (in CEF) or rtTA (in PEE) gene. This FIV-Tet-On vector system may be helpful in solving serious physiological disturbance problems which has continuously hampered successful production of transgenic animals and gene therapy.

• Journal of Animal Science and Technology
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• v.51 no.2
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• pp.97-104
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• 2009

Complement of test standard, evaluation methods and models are needed to improve national competitiveness and to exchange superior genetic resources through the comparison of genetic evaluation score among nations in dairy cattle. Therefore, this study was conducted for the application of international standard to Korea considering domestic circumstance by changing linear-classification test score system of 50 classes which is currently used in Korea to system of 9 classes which is used in advanced nations of dairy. 15,230 of holstein cow linear type records with first parity records for the fifteen linear type and one total score from 2001 to 2006 and pedigree data which were collected by the Korean Animal Improvement Association were used in this study. Population classified by 9 levels was more normal distributed than 50 levels. Correlation coefficients between 50 and 9 score system showed over 0.98 by each classification scheme. Therefore, the 50 point system can be substituted with 9 point system due to their highly positive correlation. However, scores in all traits were still very contingent on classifier under the 9 point system (p<0.001), and F values between foot angle and front teat attachment showed high fluctuation depending on classifier. It means that subjective opinions of classifier would influence on linear type score as ever even if class scheme transformed to system of 9 class. Therefore, the relevance of transformation to the 9 point system should be assessed after analyses about various environmental factors.