• Journal of Microbiology
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• v.34 no.2
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• pp.144-150
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• 1996

During growth of the genetically engineered E. coli CU103 in different media, extracellular DNAs released from the cells were studied. The extracellular DNAs released in the medium were concentrated by an thanol precipitation method and then quantified by a fluorescence method using Hoechst 33258. The released extracellular DNAs were also examined by gel electrophoresis and identified by Southern hybridization for the cloned pcbCD genes. The chromosomal DNAs and recombinant plasmid containing the cloned genes were observed to be released in an exponential growth phase. In Luria-Bertani (LB) broth and MM2-GLUCOSE, 210 and 69 ng/ml of DNAs were detected, respectively, after 3-4 days incubation at $30^{\circ}C$ and at pH 7.0. But the released DNAs were measured to be about 10-15 ng/ml in filtered river water (FW) and Tris-EDTA (TE). The at both $15^{\circ}C$ and $4^{\circ}C$, but the released DNAs were more easily degraded at the higher temperature. The extracellular DNAs were produced about 2 times more at pH 7.0 than at both pH 5.0 and pH 9.0 in MM2-glucose medium at $30^{\circ}C$. Therefore, the extracellular DNAs were found to be released actively from the cells during growth in liquid media.

• Economic and Environmental Geology
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• v.18 no.1
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• pp.55-63
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• 1985

Studies based on field observation and laboratory analysis of the littoral sands of beaches in Jeju Island indicate that the shores exhibit a great variation in both the beach geometry and the composition in terms of geological agents. Most of the beaches around the Island are developed in relatively small patches and discontinuous, as the result of intervening sea cliffs and rocky headlands. The sand quality and the dimensions of the beaches in the Island are relatively poor; for example, these beaches are 220~2,780m in length, 41~313m in width, and $7^{\circ}$ steepness in average foreshore slope. According to the textural parameters analysis, the beach sediments in study portion shows medium grained ($average\;1.42{\phi}$), moderately well sorted ($average\;0.65{\phi}$) and negative skewed ($average\;0.34{\phi}$), which seems to reflecting a high energy marine depositional environment. The heavy and light minerals of te beach sediments are composed of quartz, volcanic fragments, Na-Ca feldspar, olivine, augite as major constituents, along with apatite, biotite and other minor components, which originates from the adjacent geology. The content of CaO-MgO in shell fragments of the littoral sands ranged from 4.69~51.96%, suggesting that the high CaO-MgO content in some of the Island's beaches is attributable to geologic environments conducive to the growth of shell organisms and sediments migration. The provenance of the sediments studied are derived predominantly from adjacent continental shelves and/or terrigenous older river portion, Which sediments were transported mostly by rolling or bottom suspension. The depositional environments of the Jeju beaches can be divided into two types: beaches distributed in the North and the Northeastern parts of the Island are dominated by marine enviroment, whereas beaches in the Southwestern portion are characterized by terrigenous agencies.

• Journal of Information Technology Services
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• v.16 no.4
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• pp.15-31
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• 2017

This study analyzes 45 Korea IT service companies from 2012 to 2016 using DEA analysis. Large enterprises, medium enterprises and small and medium enterprises (SMEs). CCR model and BCC model were used for efficiency analysis. Among the various analytical objects, the decision objects which yield the maximum output with minimum input are compared with other analysis objects. The relative inefficiency was measured through this, and Technical Efficiency (TE), Pure Technology Efficiency (PTE), Scale Efficiency (SE), scale profit, reference frequency were analyzed. Also, we analyzed the Technology Gap Ratio (TGR), which is the distance between production function and Meta-Frontier for each firm, using Meta-Frontier analysis. Finally, the Tobit model is used to analyze the sources of efficiency and inefficiency. The inputs are assets, capital, and employees, and the output factor is sales. The analysis shows that large firms are achieving technological achievements more efficiently than small and medium enterprises. As a result, medium-sized enterprises and SMEs can improve efficiency overall through efficient operation of workforce and appropriate combination of inputs such as assets and capital. Also, as a result of the influence factor analysis, it was found that the ratio of the managed asset ratio and the management cost ratio were significant factors influencing the efficiency of the IT service companies. This study suggests the efficiency analysis using DEA for many Korea IT service companies. Inefficient parts of each company are classified according to size and technology. Also, we identify the most efficient companies and analyze the causes of those companies whose profits are lower than their size.

• Korean Journal of Digital Imaging in Medicine
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• v.16 no.1
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• pp.27-33
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• 2014

The purpose of this study evaluated the hemo-dynamic information within 30 seconds clinically in 3D breast MRI. From January to March 2014, A total of 40 people were examined at 1.5 Tesla(Philips, Medical System, Achieva, The Netherlands) MRI equipments using 16 channel SENSE breast coil. The imaging parameters on vibrant are fellow as: $TR/TE/FA^{\circ}$/Matrix size/Slice thickness/Slab($5ms/2ms/10^{\circ}/180{\times}139{\times}2mm/80$). This study used a Gadovist and injected it with injection speed of 4 ml /sec by auto injector with 15 ml saline flushing. Firstly, for the delay time study, it divided three different delay time from immediately, 20 seconds, and 30 seconds. In quantitative analysis, the ROI signal intensities of tumor and surrounding tissues were measured retrospectively. In qualitative analysis, the image quality was scored from 1 to 5 point by one experienced radiological technologists as a visual test. The significance level of each delay time was evaluated with a one-way ANOVA(p<0.05). In the visual test, score levels on 30 seconds delay time was a little bit higher than others(p<0.05). The signal intensity of the tumor were $1445{\pm}360$, $1410{\pm}320$, $1510{\pm}415$ on immediately, 20 seconds, and 30 seconds and score levels were $4.18{\pm}0.85$, $3.54{\pm}0.94$, $4.45{\pm}0.74$(p<0.05). The data on immediate images showed better results than that others(p<0.05). Conclusively, Although it has been high scored in 30sec delay time for visual test in order to avoid failure in 20second, 30seconds delay time after contrast media administration, we recommend that the DCE 3D breast MRI commence immediately.

• Journal of Biosystems Engineering
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• v.16 no.1
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• pp.83-89
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• 1991

One of the most serious problems in Korean sericulture farms is to improve the adiabatic conditions for the simple silkworm rearing houses which has been widely adapted since early 1980'. Thus, this study is aimed to solve the problems by selecting the thermal screen material and by finding the method of its instalation. For the study, 4 kinds of materials which are (1) TE005,(2) NW60, (3) NW300 and (4) AL110 are selected and 4 different types of methods which are surrounding boundary of rearing bed(type A), surrounding inside of wall and ceiling (type B), installing on inside of wal ony (type C) and installing on ceiling Inly (type D) are installed. Decision criteria of the best combination of screen material and installation methods is made by calculating the heating load coefficients and by testing the application at the simple silkworm rearing houses. The obtained results are summarized as follows ; 1. The effect of thermal screen on reducing the thermal energy is remarkable. It saves energy more 50% than unscreened simple silkworm rearing houses. 2. From a stand point of a good screed material on thermal energy reduction, the NW300, AL110 and PE005 are recommanded materials. However, NW60 and PE005 are regarded as desirable materials from the practical point view. 3. Also, the effect on thermal energy reduction is largely affected by the method of thermal screen installation. The surrounding boundary of the silkworm rearing bed(type A) is the most desirable method to reduce the energy, which saves 56.6% of it. 4. In the practical application, NW60 and type A is considered to be the best combination of material and installation method. By this combination, the level of maximum heating load can be reduced at the rate of 43% for spring season and 40% for autumn season. 5. Another heating method, comparted heating, can be recommended to save the energy.

• Analytical Science and Technology
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• v.13 no.6
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• pp.781-788
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• 2000

Tellurium in simulated nuclear spent fuels (SIMFUEL) has been determined by hydride generation-inductively coupled plasma atomic emission spectrometry (HG-ICP-AES). Parameters such as concentrations of HCl and $NaBH_4$, flow rate of HCl and $NaBH_4$ were optimized and then the effects of U, Mo, Pd, Rh and Ru on the Te intensity were investigated. A thiourea as a masking agent was used to eliminate or minimize such interferences specially caused by palladium. Tellurium was measured by HG-ICP-AES and ICP-MS after separation of tellurium from SIMFUEL with cation exchange chromatography. The relative deviation between direct measurement and separation method was less than 6% based on the data by ICP-MS.

• International Journal of Aeronautical and Space Sciences
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• v.16 no.4
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• pp.493-499
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• 2015

The aerodynamic performance of blunt trailing edge airfoils was investigated. The flow fields around the modified NACA64-418, which consists of the tip blade of the wind turbine and Mexico model of IEA wind, were analyzed. To imitate the repaired airfoil, the original NACA64-418 airfoil, a cambered airfoil, is modified by the adding thickness method, which is accomplished by adding the thickness symmetrically to both sides of the camber line. The thickness ratio of the blunt trailing edge of the modified airfoil, $t_{TE}/t_{max}$, is newly defined to analyze the effects of the blunt trailing edge. The shape functions describing the upper and lower surfaces of the modified NACA64-418 with blunt trailing edge are obtained from the curve fitting of the least square method. To verify the accuracy of the present numerical analysis, the results are first compared with the experimental data of NACA64-418 with high Reynolds number, $Re=6{\times}10^6$, measured in the Langley low-turbulence pressure tunnel. Then, the aerodynamic performance of the modified NACA64-418 is analyzed. The numerical results show that the drag increases, but the lift increases insignificantly, as the trailing edge of the airfoil is thickened. Re-circulation bubbles also develop and increase gradually in size as the thickness ratio of the trailing edge is increased. These re-circulations result in an increase in the drag of the airfoil. The pressure distributions around the modified NACA64-418 are similar, regardless of the thickness ratio of the blunt trailing edge.

• Applied Microscopy
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• v.22 no.2
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• pp.66-74
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• 1992

In an attempt to produce transgenic amphibia, bacteriophage ${\lambda}-DNA$ was microinjected into fertilized eggs of Xenopus laevis, and the effect on early embryogenesis and the ultrastructural behavior of exogenous DNA were investigated. The effect of microinjected gene on embryonic development showed differences according to the concentration of injected DNA and the incubation temperature. Various concentrations of ${\lambda}-DNA$ were tested. Among those, microinjection of 1-2 ng DNA dissolved in 20 nl TE buffer was not shown to disturb normal embryonic development and was recorded the highest survivability to the late tadpole stage (Stage 43); however, injection of increased concentrations of DNA than above provoked irregular cleavages or abnormal appearances, which resulted in reduced survivability. When the injected embryos were incubated at low temperatures (e.g., $12^{\circ}C$), 54.5% of the embryos developed to Stage 43, whereas 42.4% survived when incubated at room temperature. The survivability showed also differences according to the injection site. 58.0% of the embryos developed to Stage 43 when microinjected into the vegetal pole, whereas 44.9% survived when microinjected into the animal pole. To understand the structural fate or behavior of injected DNA a combined light and electron microscopical study was applied. The nucleus-like structure was observed in the ${\lambda}$ DNA-injected embryos, which was quite a similar to the interphase nuclei of normal Xenopus laevis. The nucleus-like structure showed the typical double-layered nuclear membrane and nuclear complexes; however, it consisted of unusual structures such as furrows of nuclear envelope into the nucleoplasm.

• Journal of Biomedical Engineering Research
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• v.16 no.4
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• pp.481-491
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• 1995

Endovaginal and endorectal receiver only surface coils were designed for MR imaging (MRI) and $^1H$ MR spectroscopy (MRS) for the uterine cervix and the prostate. The shape of endovaginal coil wire was rectangular with round corner. Size of the coil wire was empirically determined for 7cm and 4cm along the long and short axis, respectively. The coil wire loop was supported by acryl handle and bent about $150^{\circ}$ at one side of the loop considering the average angle of the cervix to the vagina. We called this as a "spoon-type endovaginal coil". The wire of the endorectal coil was made of the flexible materials so that the wire loop became long elliptic shape by pushing the acryl handle into the plastic tube for the comfort of patients when the coil was inserted into the cervix. Then, the shape was maintained to be circle by popping out handle. Conventional spin echo (SE) and fast spin echo (FSE) sequences were used as 71 and 72 weighted imaging sequences, respectively. Matrix size was 128~$256{\times}256$. FOVs for surface coil and body coil were 14cm and 24cm, respectively. 3D volume localized in vivo $^1H$ MR spectroscopy of the human cervix and prostate was performed using PRESS or STEAM localization method with the following parameters . TR=3 sec, TE=135 msec for PRESS or 30 msec for STEAM, NEX=2, NS=48, Sl=2048, and SW=2500 Hz. Using home-built endovaginal and endorectal coils, excellent T1- and T2-images were obtained to visualize early cervical and prostate tumors. 3D volume localized in vivo IH MRS was useful to differentiate the cancerous tissue from the normal tissue.

• Korean Journal of Acupuncture
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• v.25 no.3
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• pp.81-96
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• 2008

Objectives : The purpose of the present study is to determine the effective analgesic parameters of manual acupuncture (MA) and/or electroacupuncture (EA), thus how MA & EA of specific acupoint works after acute ankle sprain in rats. Thus, one series of experiments was designed to analyze the analgesic effects of acupuncture in either the normal or sprained rat, by comparing the behavioral features before and after MA or EA at several acupoints. Methods : Ankle sprain was induced manually by hyper-extension of ligaments of the right ankle in rat. Either MA or EA was applied to the LI4, TE17, 足三里 and SI-6 acupoints at an intensity of 2 mA (1 ms pulse duration) with a frequency of 10 Hz for 30 minutes. Results : Behaviorally, manual acupuncture or electroacupuncture produced potent analgesic effects on acute ankle sprained animals as measured by weight bearing capacity of the affected limb. Furthermore, antinociceptive effect of EA was suppressed by alpha adrenoceptor antagonist phentolamine (5 mg/kg, i.p.) but not by opioid antagonist naltrexone (10 mg/kg, i.p.). Conclusions : It is suggested that acupuncture-induced antinociception may involve the descending inhibition of ${\alpha}2$-adrenoceptor.