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Continuous Production Process of Methyl Fructoside Using Alginate-enclosed Microspheres (Alginate-enclosed Microspheres를 이용한 메틸 프룩토시드의 연속생산공정)

  • 허주형;김해성
    • KSBB Journal
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    • v.10 no.2
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    • pp.159-165
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    • 1995
  • Methyl fructoside was continuously produced in suspended bed enzyme reactor using alginate-enclosed microspheres biocatalyst which was developed for enzymatic synthesis of methyl fructoside. And the continuous operating conditions were optimized with reactor simulation in order to demonstrate a feasibility of commercialization of the continuous enzymatic production process development. The yield and productivity of methyl fructoside were as high as 47.1%o and $2g/\ell$-hr, respectively. The optimum operating conditions were pH 4.8, 30%(v/v) of methanol content and $2U/m\ell$ of enzyme activity when the initial concentration of sucrose is $0.291mo1/\ell$ at the reaction temperature of $25^{\circ}C$.

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Isolation and characterization of the outer membrane vesicle (OMV) protein from Vibrio anguillarum O1 (Vibrio anguillarum O1이 생산하는 Outer Membrane Vesicle (OMV)의 분리 및 OMV 내의 단백질 특성)

  • Hong, Gyeong-Eun;Kim, Dong-Gyun;Min, Mun-Kyeong;Kong, In-Soo
    • Journal of Marine Bioscience and Biotechnology
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    • v.2 no.2
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    • pp.123-125
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    • 2007
  • Vibrio anguillarum is a gram-negative bacterium that causes vibriosis in approximately 80 different fish species. V. anguillarum produces several exotoxins are correlated with the pathogenesis of vibriosis. This study is focused on the composition of the outer membrane vesicle. Most of gram-negative bacteria produce outer membrane vesicle (OMV) during cell growth. OMV was formed from the outer membrane surface of cell and than released to extracellular environment. OMV consists of outer membrane lipids, outer membrane protein (OMP), LPS, and soluble periplasmic components. Also, they contain toxins, adhesions, and immunomodulatory. Many gram-negative bacteria were studied out forming OMV. In Vibrio sp., formation of OMV by electron microscopy has been reported from V. cholerae and V. parahaemolyticus. In present study, we isolated OMV from V. anguillarum and OMV protein was separated by SDS-PAGE. Magor band was sliced and analyzed by MALDI-TOF. The major protein band of 38kDa was identified as OmpU by MALDI-TOF MS analysis.

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The Effect of Forebody Forms on the Ship Motion in Regular Head Waves (선형(船型)이 선체운동(船體運動)에 미치는 영향(影響)에 관(關)한 연구(硏究))

  • Jin-Ahn,Kim
    • Bulletin of the Society of Naval Architects of Korea
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    • v.12 no.1
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    • pp.47-58
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    • 1975
  • The effect of the bow shape on the ship motion response among longitudinal regular waves, is investigated employing the strip theory. The two dimensional hydrodynamic forces such as added mass and damping are calculated by the integral equation method for arbitrary sections. Nine ship models are selected for investigation. They are U, UV and V bow ship forms of different block coefficient of 0.6, 0.7 and 0.8 with constant after body. The heave amplitude of the V bow ship is smaller than that of the U bow ship in the whole range of wave length except extremely short wave as were stated by the earlier investigators. This results holds also in the case of bow vertical motions such as vertical relative displacement, velocity and acceralation. As to the pitch amplitudes, the V bow ship gives smaller value in long waves but larger value in short waves. However, heave and pitch phase angles are practically not influenced by the form of the fore body sections. In the bow motions, a little difference in phase angle is appeared in the vicinity of the wave which has same ship length. With respect to the wave exiting force and moment unfovourable effects could be expected in V bow ships. And these tendency hold also in the wave bending moment.

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THERMAL DIFFUSION AND RADIATION EFFECTS ON UNSTEADY MHD FREE CONVECTION HEAT AND MASS TRANSFER FLOW PAST A LINEARLY ACCELERATED VERTICAL POROUS PLATE WITH VARIABLE TEMPERATURE AND MASS DIFFUSION

  • Venkateswarlu, M.;Ramana Reddy, G.V.;Lakshmi, D.V.
    • Journal of the Korean Society for Industrial and Applied Mathematics
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    • v.18 no.3
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    • pp.257-268
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    • 2014
  • The objective of the present study is to investigate thermal diffusion and radiation effects on unsteady MHD flow past a linearly accelerated vertical porous plate with variable temperature and also with variable mass diffusion in presence of heat source or sink under the influence of applied transverse magnetic field. The fluid considered here is a gray, absorbing/emitting radiation but a non-scattering medium. At time t > 0, the plate is linearly accelerated with a velocity $u=u_0t$ in its own plane. And at the same time, plate temperature and concentration levels near the plate raised linearly with time t. The dimensionless governing equations involved in the present analysis are solved using the closed analytical method. The velocity, temperature, concentration, skin-friction, the rate or heat transfer and the rate of mass transfer are studied through graphs in terms of different physical parameters like magnetic field parameter (M), radiation parameter (R), Schmidt parameter (Sc), Soret number (So), Heat source parameter (S), Prandtl number (Pr), thermal Grashof number (Gr), mass Grashof number (Gm) and time (t).

Improvement of Cellulase Activity Using Error-Prone Rolling Circle Amplification and Site-Directed Mutagenesis

  • Vu, Van Hanh;Kim, Keun
    • Journal of Microbiology and Biotechnology
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    • v.22 no.5
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    • pp.607-613
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    • 2012
  • Improvement of endoglucanase activity was accomplished by utilizing error-prone rolling circle amplification, supplemented with 1.7 mM $MnCl_2$. This procedure generated random mutations in the Bacillus amyloliquefaciens endoglucanase gene with a frequency of 10 mutations per kilobase. Six mutated endoglucanase genes, recovered from six colonies, possessed endoglucanase activity between 2.50- and 3.12-folds higher than wild type. We sequenced these mutants, and the different mutated sites of nucleotides were identified. The mutated endoglucanase sequences had five mutated amino acids: A15T, P24A, P26Q, G27A, and E289V. Among these five substitutions, E289V was determined to be responsible for the improved enzyme activity. This observation was confirmed with site-directed mutagenesis; the introduction of only one mutation (E289V) in the wild-type endoglucanase gene resulted in a 7.93-fold (5.55 U/mg protein) increase in its enzymatic activity compared with that (0.7 U/mg protein) of wild type.

Automatic Building Extraction Using SpaceNet Building Dataset and Context-based ResU-Net (SpaceNet 건물 데이터셋과 Context-based ResU-Net을 이용한 건물 자동 추출)

  • Yoo, Suhong;Kim, Cheol Hwan;Kwon, Youngmok;Choi, Wonjun;Sohn, Hong-Gyoo
    • Korean Journal of Remote Sensing
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    • v.38 no.5_2
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    • pp.685-694
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    • 2022
  • Building information is essential for various urban spatial analyses. For this reason, continuous building monitoring is required, but it is a subject with many practical difficulties. To this end, research is being conducted to extract buildings from satellite images that can be continuously observed over a wide area. Recently, deep learning-based semantic segmentation techniques have been used. In this study, a part of the structure of the context-based ResU-Net was modified, and training was conducted to automatically extract a building from a 30 cm Worldview-3 RGB image using SpaceNet's building v2 free open data. As a result of the classification accuracy evaluation, the f1-score, which was higher than the classification accuracy of the 2nd SpaceNet competition winners. Therefore, if Worldview-3 satellite imagery can be continuously provided, it will be possible to use the building extraction results of this study to generate an automatic model of building around the world.

Automatic Pancreas Detection on Abdominal CT Images using Intensity Normalization and Faster R-CNN (복부 CT 영상에서 밝기값 정규화 및 Faster R-CNN을 이용한 자동 췌장 검출)

  • Choi, Si-Eun;Lee, Seong-Eun;Hong, Helen
    • Journal of Korea Multimedia Society
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    • v.24 no.3
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    • pp.396-405
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    • 2021
  • In surgery to remove pancreatic cancer, it is important to figure out the shape of a patient's pancreas. However, previous studies have a limit to detect a pancreas automatically in abdominal CT images, because the pancreas varies in shape, size and location by patient. Therefore, in this paper, we propose a method of learning various shapes of pancreas according to the patients and adjacent slices using Faster R-CNN based on Inception V2, and automatically detecting the pancreas from abdominal CT images. Model training and testing were performed using the NIH Pancreas-CT Dataset, and intensity normalization was applied to all data to improve pancreatic detection accuracy. Additionally, according to the shape of the pancreas, the test dataset was classified into top, middle, and bottom slices to evaluate the model's performance on each data. The results show that the top data's mAP@.50IoU achieved 91.7% and the bottom data's mAP@.50IoU achieved 95.4%, and the highest performance was the middle data's mAP@.50IoU, 98.5%. Thus, we have confirmed that the model can accurately detect the pancreas in CT images.

Advanced P-Channel Poly-Si TFTs for SOG

  • Park, Seong-Jin;Kang, Sang-Hoon;Ku, Yu-Mi;Choi, Jong-Hyun;Jang, Jin
    • 한국정보디스플레이학회:학술대회논문집
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    • 2004.08a
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    • pp.1019-1022
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    • 2004
  • High performance p-ch poly-Si TFTs with excellent stability were developed. By using a frequency doubled DPSS CW laser, the a-Si on glass could be crystallized into one dimensional single crystalline silicon named as a sequential lateral crystallization (SLC) region. We fabricated p-ch TFTs on SLC region and the typical characteristic values of the TFTs were $u_{fe}$ = 180 $cm^2$/Vs, $V_{th}$ = -3 V, S.S. = 0.5 V/dec, and $I_{off}$ = 1 pA/um@ $V_d$ = -10V. It is found that the TFTs are very stable after bias stresses such as negative and positive gate biases, hot carrier bias and high current bias. These results indicate that the poly-Si in SLC region is suitable for system on glass (SOG) application.

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A study on Virtual-City system based on 3D-Web GIS for the disaster prevention of U-Eco city (U-Eco City내 체계적인 방재를 위한 3D-Web GIS기반의 가상도시 시스템 방안 연구)

  • Jo, Byung-Wan;Kim, Do-Keun;No, Seung-Hyun;Yoon, Kwang-Won
    • 한국방재학회:학술대회논문집
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    • 2008.02a
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    • pp.661-664
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    • 2008
  • U-Eco City is promoted nation-wide by development of recent IT technology, method of effective countermeasure disasters, which real-time wire/wireless communication network, and 3D-Web GIS shall be connected that massive database, intelligent service be perceived. U&V-City is the four-dimensional future city that actualizes an intelligent daily ubiquitous computing service by embodying 3D-Virtual City, the reproduction of real world U-City while using Digital map, satellite image, VRML(Virtual Reality Modeling Language), which are presentation tool to describe city components and by efficiently catch and cope about nature and human disasters while employing EAI(External Authoring Interface) that provides HTML&JAVA, and interface for efficient removal/process of massive information/service and also by employing GPS/LBS/Navigation in support of the world-wide orientation concept, and RTLS(Real Time Location System).

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ON THE WEAK LAWS WITH RANDOM INDICES FOR PARTIAL SUMS FOR ARRAYS OF RANDOM ELEMENTS IN MARTINGALE TYPE p BANACH SPACES

  • Sung, Soo-Hak;Hu, Tien-Chung;Volodin, Andrei I.
    • Bulletin of the Korean Mathematical Society
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    • v.43 no.3
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    • pp.543-549
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    • 2006
  • Sung et al. [13] obtained a WLLN (weak law of large numbers) for the array $\{X_{{ni},\;u_n{\leq}i{\leq}v_n,\;n{\leq}1\}$ of random variables under a Cesaro type condition, where $\{u_n{\geq}-{\infty},\;n{\geq}1\}$ and $\{v_n{\leq}+{\infty},\;n{\geq}1\}$ large two sequences of integers. In this paper, we extend the result of Sung et al. [13] to a martingale type p Banach space.