• Food Industry
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• s.181
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• pp.68-91
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• 2004

조미료란 식품을 조리, 가공, 섭취할 때 맛을 증진시키거나 강화시킬 목적으로 사용되는 물질을 일컫는다. 발효, 종합 및 천연계 조미료의 국내외 시장동향에 대해 살펴보고, 조미료의 향후 발전방향과 중점 연구 분야, 관련업계의 연구개발 동향에 대한 고찰을 토대로 조미료 분야의 고부가가치 산업으로의 육성방안에 대해 알아보았다. 미원, 아이미 등으로 대표되는 발효조미료의 내수시장은 성숙기를 거쳐 소폭 감소세를 보이는 쇠퇴기에 접어들고 있다. 이는 소비자의 MSG에 대한 기피현상이 주 원인으로 판단된다. 전반적으로 품질 차별화 없이 가격 경쟁이 심화되고 있는 실정이다. 국내 종합조미료 시장은 1975년 국내에 처음으로 CJ에서 다시다 제품을 출시한 후 대상에서 1982년 맛나, 1988년 감치미를 출시하면서 30여 년간 지속적으로 성장해 왔다. 현재 시장규모는 2천억원에 이르고 있으며 최근 들어서는 성장세가 완만해지고 있고 가정용은 거의 정체되어 있다. 일본 조미료 시장은 발효조미료, 종합조미료 그리고 다양한 조미식품의 소재로 활용되는 천연조미료로 구분할 수 있다. 발효조미료 시장은 국내와 비슷한 상황으로 전체적인 물량변화는 약간 감소하는 경향이며 생산기지의 해외이전 현상이 뚜렷하다. 종합조미료 시장은 한국과 유사한 풍미조미료 외에도 보다 전문화되고 다양한 형태의 조미료가 시판되고 있으며, 전통적인 풍미조미료는 정체현상을 보이고 건강지향, 전용화 조미료가 호조를 보이고 있다. 일본과 한국의 조미료 발전 단계는 조금 차이를 보이고 있다. 현재 한국은 종합조미료 시대에서 용도별 조미료 시대로 넘어가는 단계에 있는 반면, 일본은 이미 메뉴 전용 조미료시대에 접어들었고, 건강, 기능성을 강조하는 시대로 나아가고 있다. 이들 변화는 건강에 대한 소비자의 관심과 그로 인한 보다 천연에 가까운 조미소재에 대한 수요, 동시에 맛에 대한 충족까지 요구하는 시대적 변화에 따른 필연적 결과로 보인다. 미래의 조미료는 천연계 소재의 사용량이 증가할 것이고, 소비자의 건강, 안전 추구심리에 부흥하여 안전성과 기능성을 부여하는 소재에 관심이 집중될 것으로 보인다. 더불어 전반적인 생활수준 향상에 따라 제품의 고급화와 전용화도 진행될 것으로 보인다. 향후 조미료시장을 주도할 각 현상에 대해 좀더 자세히 알아보고 학계 및 업체의 최근 연구동향과 제품개발 현황에 대해 고찰해 보았다. 기술연구 동향을 보면, 나노 기술에 의한 조미신소재연구, 초미분쇄기술, 미세 캡슐화 기술, 생물공학 기술이 활용된 발효 및 효소분해에 의한 펩타이드계 조미소재 개발 등이 활발하게 진행되고 있다. 선진업체 연구 및 제품개발 동향은 아지노모트/교와의 ''코쿠미''계 조미소재 연구개발, 일연푸드/동해물산의 천연 엑기스계 조미소재 연구개발, 보조주(주)의 주류 조미료 개발 위주로 살펴보았다. 조미료 시장의 발전 과정과 선진 업체들의 연구, 개발동향을 종합해 보면 첫째는 천연 건강지향에 부응하는 차별화된 소재 및 기능성 소재 개발이고 둘째는 맛과 편리함의 추구에 대응하는 메뉴 전용화 추세이다. 이를 위한 기술적 연구도 기능성 소재(히스티딘 함유 디펩타이드)개발, 나노기술, 미세캡슐화 기술 등을 통하여 다양하게 진행되고 있다. 조미료 산업을 첨단 고부가가치 산업으로 육성하기 위해서는 R&D 투자확대와 효율성을 제고하는 것이 필수적이다. 각 기업단위에서는 장기적 차원에서 첨단식품 기반기술에 대한 연구를 수행하거나, 전문 중소기업, 벤처를 육성하는데 지원해야 한다. 그러나 현실은 당장의 수익과 회사간 경쟁으로 인해 장기적 투자, 사회적 인프라 육성 등에는 관심을 쏟지 않고 있다. 조미료를 비롯한 식품에 관련된 기술들이 다양한 기초연구와 응용기술, 개발연구 등의 총합인 경우가 많기 때문에 이들 상이한 연구 분야간의 연계를 강화하고 개발된 기술의 이전, 확산체계를 구축할 필요가 있다. 이를 위해 기업간 수평적 네트워크 외에도 국가기관이 참여하는 연구 자원의 효율적 집행 기관이 필요하리라 본다.

• Journal of the Korean Chemical Society
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• v.54 no.2
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• pp.208-214
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• 2010

In the presence of alcohol, phospholipase D (PLD) is known to perform transphosphatidylation activity, during which the overall reaction rate of PLD increased. To elucidate the reaction mechanism of transphosphatidylation further, we investigated rate constants of transphosphatidylation reaction of the purified ${\alpha}$-type PLD from cabbage in the presence of various alcohols. The second-oder rate constants of PLD transphosphatidylation showed a large increase with the primary alcohols examined as expected. In the case of butanol we observed the second-oder rate constant of $33.33{\pm}1.33M^{-1}sec^{-1}$. This second-order rate constant of transphosphatidylation was as 400 times greater as the second-order hydrolysis rate constant of $0.078M^{-1}sec^{-1}$ which was adjusted for the water concentration. A linear free energy relationship between the $pK_a$ of alcohol and transphosphatidylation rate gives a Br${\o}$nsted slope of ${\beta}_{nu}$ = 0.12 ${\pm}$ 0.03. This small ${\beta}_{nu}$ value implicates that the transition state of break down of phosphatidyl-enzyme intermediate (E-P) is likely dissociative. Finally, a reaction mechanism of cabbage PLD is suggested on the basis of our results presented here and the histidine residue known to be located in the active site of cabbage PLD.

• Journal of the Korean Chemical Society
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• v.33 no.1
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• pp.3-10
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• 1989

In order to obtain a clue in understanding enzymatic hydrolysis in which the His-Cys moieties of papain protease is involved, we prepared cationic peptide-sufactants bearing histidyl, cysteinyl, and both histydyl and cysteinyl residues. Their catalytic efficiency toward the hydrolysis of PNPL were investigated in comicellar phases formed with $N^{+}C_{2}CysC_{12}$, $N^{+}C_{2}HisC_{12}$, $N^{+}C_{2}HisCysC_{12}$ increased markedly in the same order compared with that of $N^{+}C_{2}AlaC_{12}$. The markedly increased catalytic effects are attributed to the imidazole groups of $N^{+}C_{2}HisC_{12}$ and the thiol groups of $N^{+}C_{2}CysC_{12}$, and the large catalytic efficiency of $N^{+}C_{2}HisCysC_{12}$, is considered due to the interaction of the imidazole and the thiol groups. In order to investigate catalytic activities, rate constants for the functional groups, km* and dissociation constants, pKa have been determined. The results showed that $k^{\ast}_m$ and pKa of the imidazole groups were $7.91{\times}10^{-4}S^{-1}$ and 6.49, and those of the thiol groups were $6.00{\times}10^{-4}S^{-1}$ and 10.50. The catalytic effects of comicellar systems on the hydrolysis of p-nitrophenyl esters has increased according to the increasing size of the alkyl carbon number. Therefore, the catalytic effects have been increasing by the interaction of micellar hydrophobic parts and substrates as well as action of the functional groups.

• Korean Journal of Plant Resources
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• v.27 no.1
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• pp.35-42
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• 2014

Correlation and similarity of garlic cultivars was analyzed by measuring the composition of twenty amino acids contents in the bulb and callus from twelve garlic cultivars which were collected from Korea and foreign countries. Arginine and asparagine occupied more than 78% of total amino acids both in bulb and callus followed by glutamic acid, lysine, aspartic acid, valine, glycine, histidine and ornithine in order. Based on correlation coefficients of amino acid composition in the bulb, twelve garlic cultivars were classified into three major groups. Danyang, Euiseong, Seosan, Jeju, Mongol and Turkey cultivars belong to group-I, Namdo, China, Mexico and Nepal cultivars are in group-II, and group-III includes Philippine and Daeseo cultivars. Based on amino acid composition in callus, group-I includes Mongol, Euiseong, Danyang and Seosan cultivars, group-II includes Jeju, Daeseo, China and Namdo cultivars, group-III includes Napal, Mexico, Philippine and Turkey cultivars. Composition of amino acid contained in both callus and bulb is a clear standard to identify northern-type garlic cultivars. Especially the composition of amino acids in callus is more distinctive standard of classification between northern and southern type garlic cultivars than that in bulbs.

• Journal of Life Science
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• v.33 no.11
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• pp.956-965
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• 2023

In plants, there are many CCCH zinc finger proteins consisting of three cysteine residues and one histidine residue, which bind to zinc ions with finger configuration. CCCH-type zinc finger proteins are divided into tandem CCCH-type zinc finger (TZF) and non-TZF proteins: TZF proteins contain exactly two tandem CCCH-type zinc finger motifs whereas non-TZF proteins have fewer or greater than two CCCH-type zinc finger motifs. The functions of TZF genes, especially plant-specific RR-TZF genes, have been well studied in several plants, whereas the functional roles of non-TZF genes have not been adequately researched compared to TZF genes. Many non-TZF genes have been identified as being involved in the responses to biotic and abiotic stresses, such as pathogen, high salt, drought, cold, heat, and oxidative stresses. Some non-TZF proteins bind to RNA and are involved in the post-transcriptional regulation of stress-responsive genes in the cytoplasm. In addition, other non-TZF proteins act as transcriptional activators or repressors that regulate the expression of stress-responsive genes in the nucleus. Despite these studies, stress signal transduction and upstream and downstream genes of non-TZF genes have not been sufficiently researched, suggesting that additional studies of the functions of non-TZF genes' functions in plants' stress responses are needed. In this review, we describe non-TZF genes involved in biotic abiotic stress responses in plants and their molecular functions.

• Journal of Periodontal and Implant Science
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• v.33 no.4
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• pp.747-753
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• 2003

면역글로불린의 Fc 부분에 대한 수용기인 $Fc{\gamma}R$는 세균에 대한 인식, 결합과 포식작용과정에서 중요한 역할을 한다. 이 $Fc{\gamma}R$에서 $Fc{\gamma}R$IIa, $Fc{\gamma}R$IIIa, $Fc{\gamma}R$IIIb의 유전자 다형성이 치의학 분야에서 연구되고 있다. $Fc{\gamma}R$IIa에서는 두 번째 세포외 면역글로불린 유사 영역의 131번째 아미노산에서 아르기닌($Fc{\gamma}R$IIa-R131) 혹은 히스티딘($Fc{\gamma}R$IIa-H131)을 갖고 있으며, $Fc{\gamma}R$IIIa에서는 두번째 세포외 영역의 158번째 아미노산이 발린($Fc{\gamma}R$IIIa-158V) 혹은 페닐알라닌($Fc{\gamma}R$IIIa-158F)을 갖고 있다. $Fc{\gamma}R$IIIb에서는 첫 번째 세포외 면역글로불린 유사영역의 4개의 아미노산의 유전자 다형성으로 인해서 $Fc{\gamma}R$IIIb-NA1과 $Fc{\gamma}R$IIIb-NA2의 두가지 유전자 다형성을 보이고 있다. 이번 연구는 치주적으로 건강한 한국인에서 $Fc{\gamma}R$IIa, $Fc{\gamma}R$IIIa, $Fc{\gamma}R$IIIb에 대한 유전자형의 분포를 조사하고자 한 것으로 서울대학교 치과병원에 근무하는 치과의사, 치과위생사, 간호조무사 및 서울대학교 치과대학 4학년 학생 중 치주낭 깊이와 부착소실이 4mm 이하인 치주적으로 건강한 한국인 65명을 대상으로 하였다. $Fc{\gamma}R$IIa, $Fc{\gamma}R$IIIa, $Fc{\gamma}R$IIIb의 유전자 다형성은 분리한 DNA에 각 대립유전자에 특이성을 지닌 primer를 넣고 PCR(polymerase Chain Reaction)법을 이용하여 증폭시킨후 전기영동법을 이용하여 각 대립유전자의 존재를 확인함으로써 결정하였다. $Fc{\gamma}R$IIa의 유전자 다형성은 R/R131, R/H131, H/H131의 유전자형에 대하여 각각 7.7%, 38.5%, 53.8%의 분포를 보였으며, $Fc{\gamma}R$IIIa의 158V/V, 158V/F, 158F/F 유전자형에 대하여 각각 7.7%, 35.4%, 56.9%의 분포를 보였다. 또한 $Fc{\gamma}R$IIIb의 NA1/NA1, NA1/NA2, NA2/NA2 유전자형은 각각 33.9%, 53.8%, 12.3%의 분포를 보였다. 이를 바탕으로 각 대립유전자의 발생빈도 계산한 결과 $Fc{\gamma}R$IIa의 R131과 H131이 26.9% 73.1%로 나타났으며, $Fc{\gamma}R$IIIa의 158V, 158F의 유전자형이 25.4%, 74.6%로 나타났다. $Fc{\gamma}R$IIIb의 NA1, NA2 유전자형의 발생빈도는 60.8%, 29.2%로 나타났다. 이번 연구는 치주적으로 건강한 한국인에서의 $Fc{\gamma}R$IIa, $Fc{\gamma}R$IIIa, $Fc{\gamma}R$IIIb에 대한 유전자형의 분포를 조사한 것으로, 이후 치주질환자의 유전자형 분포와의 비교로 치주질환과 $Fc{\gamma}R$IIa, $Fc{\gamma}R$IIIa, $Fc{\gamma}R$IIIb의 유전자다형성과의 관련성에 관한 추가적인 연구가 필요할 것으로 여겨진다.

• Journal of the Korean Society of Food Science and Nutrition
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• v.45 no.10
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• pp.1406-1413
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• 2016

This study was carried out to evaluate the toxicity of ethanolic extracts of Morus alba L. branch (ME). In the reverse mutation test, Salmonella Typhimurium TA98, TA100, TA1535, TA1357, and Escherichia coli WP2uvrA were used to estimate the mutagenic potential of ME. Sprague-Dawley rats were orally administered ME at levels of 1,250, 2,500, and 5,000 mg/kg for the single-dose toxicity test and 500, 1,000, and 2,000 mg/kg/d for the repeated-dose toxicity test for 28 consecutive days. As expected, reverse mutation was not detected at any concentration of ME, regardless of application of the metabolic activation system with or without S9 mix. In the single-dose toxicity test, ME caused neither significant visible signs of toxicity nor mortality in rats, and $LD_{50}$ was estimated to be over 5,000 mg/kg. In the repeated-dose toxicity test, ME administration at 500, 1,000, and 2,000 mg/kg for 28 days to male or female rats did not result in mortality. Similarly, no toxicologically significant treatment-related changes in body weight, food intake, or organ weights were noted. Several hematological and biochemical parameters in both genders showed significant differences, but these were within normal ranges. These results support the safe use of ME.

• Applied Microscopy
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• v.41 no.1
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• pp.47-53
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• 2011

Hair is an appendage of skin which protects the body from outer physical and chemical stimuli. Hair is generated from the hair follicle lying on a sunken basal layer of epidermis. Hair cycling, which regenerates hair follicles throughout the life time of the organism. Numerous kinds of factors which exist at the hair follicle have been reported to regulate hair cycling, Human growth hormone secreted from pituitary gland, initially demonstrated to accelerate organ's growth, has been reported to play a role in the biology of organ size determination. We investigated the effect of 6-histidines residues tagged at amino-terminus of human growth hormone using light and electronmicroscopic methods. Human growth hormone encapsulated in nano-liposome (LhGH) was used to find how LhGH affects hair follicle cycling of mouse (C57BL6/CrN). Distilled water as a negative control, 3% Minoxidil as a positive control, and LhGH were applied to mouse for weeks. LhGH increased the number of exposed hairs per given areas ($1mm^2$). This result was also confirmed using a different breed of mice which show natural hair loss in an old age (about 17 months after birth). When LhGH was applied for 3 weeks after natural hair loss, natural hair loss on these mice was prevented, However, the control group mice on which LhGH was not applied showed further hair loss. This result indicates that LhGH may stimulate hair cycling of mouse. In clusion, it is cleat that the LhGH increased the number of hair on mice and help the depilated skin to grow new hair follicles again.

• Journal of Life Science
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• v.21 no.7
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• pp.1032-1038
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• 2011

Recently identified Ciona intestinalis voltage sensor-containing phosphatase (Ci-VSP) consists of an ion channel-like transmembrane domain (VSD) and a phosphatase-like domain. Ci-VSP senses the change of membrane potential by its VSD and works as a phosphoinositide phosphatase by its phosphatase domain. In this study, we present the construction of His-tagged phosphatase-like domain of Ci-VSP, its recombinant expression and purification, and its enzymatic activity behavior in order to examine the biochemical behavior of phosphatase domain of Ci-VSP without interference. We found that Ci-VSP(248-576)-His can be eluted with an elution buffer containing 25 mM NaCl and 100 mM imidazole during His-tag purification. In addition, we found the proper measurement condition for kinetics study of Ci-VSP(248-576)-His against p-nitrophenyl phosphate (pNPP). We measured the kinetic constant of Ci-VSP(248-576)-His at $37^{\circ}C$, pH 5.0 or 5.5, under 30 min of reaction time, and less than $2.0\;{\mu}g$ of protein amount. With these conditions, we acquired that Ci-VSP(248-576)-His has $K_m$ of $354{\pm}0.143\;{\mu}M$, $V_{max}$ of $0.0607{\pm}0.0137\;{\mu}mol$/min/mg and $k_{cat}$ of $0.359{\pm}0.009751\;min^{-1}$ for pNPP dephosphorylation. Therefore, we produced a pure form of Ci-VSP(248-576)-His, and this showed a higher activity against pNPP. This purified protein will provide the road to a structural investigation on an interesting protein, Ci-VSP.

• Journal of the Korean Society of Food Science and Nutrition
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• v.37 no.6
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• pp.784-791
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• 2008

A new sake fermented using a mixture of Aspergillus sp., Rhizopus sp. and yeast (Saccharomyces cerevisiae) containing allbanggae was developed in this study. The effects of the allbanggae contents ($0{\sim}50%$) in the sake on the qualitative and sensory properties and flavor components were investigated. The fermentation with allbanggae, especially in sake containing 10% allbanggae, showed the highest level of alcohol ($15.3{\sim}16.4%$), whereas the amount of alcohol decreased with increasing allbanggae contents. Amino acidity in sake containing 10% and 20% allbanggae indicated the highest levels ($0.90{\sim}1.20%$). In addition, it was observed that the sake containing 10% allbanggae showed the smallest level of reducing sugars, which were increased with increasing allbanggae ratio. Furthermore, various organic acids in the sake were detected such as citric, succinic, malic and acetic acids, among which the acetic acid showed the highest amount ($160.3{\pm}8.0{\sim}253.3{\pm}20.3mg/100mL$). The major amino acids detected in the sakes were alanine, proline, histidine, phenylalanine, lysine and glutamic acid. The sake containing 20% allbanggae indicated the highest amino acids amount among the allbanggae ratios. Based on these results, it was concluded that the sake containing 20% allbanggae could be successfully commercialized.