• KSBB Journal
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• v.24 no.2
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• pp.122-130
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• 2009

Anti-angiogenic mechanism was examined for anti-obesity agents with the extract of P.radix, P.semen, S.hebra and C.furctus through anti-cell adhesion effect and western blot. Cell adhesion molecules, VCAM-1 was supressed with the order of P.radix (0.2 ppm, 125%) > P.semen (0.5 ppm, 100%) > S.hebra (5.0 ppm, 114%) > C. furctus (5.0 ppm, 111.8%), ICAM-1 was inhibited by P.radix (0.25 ppm, 130%) > P.semen (0.5 ppm, 100%) > S.hebra (5.0 ppm, 138%) > C. furctus (5.0 ppm, 66.7%), E-Selectin was also supressed P.radix (0.25 ppm, 100%) > P.semen (1.0 ppm, 128%) > S.hebra (5.0 ppm, 120%) > C. furctus (5.0 ppm, 100.7%). And signal molecules, VE-cadherin was supressed by P.radix and S.hebra, ${\beta}$-catenin was inhibited by P.radix, and Akt was supressed all these 4 kinds of natural products. These P.radix, P.semen, S.hebra and C.furctus were showed the possibility of anti-obesity agents based on anti-angiogenesis.

• Korean Journal of Pharmacognosy
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• v.5 no.1
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• pp.17-29
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• 1974

• KSBB Journal
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• v.24 no.6
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• pp.541-548
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• 2009

For investigation of anti-angiogenesis mechanism of Anthrisci radix, Psoraleae semen, Siegesbeckiae herba and Corni fructus, anti-cell adhesion experiment was performed. The adhesion of U937 cells to IL-$1\beta$-stimulated HUVECs was completely suppressed by 276% at 0.2 mg/L of Anthrisci radix, 220% at 5 mg/L of Psoraleae semen, 158% at 10 mg/L of Siegesbeckiae herba and 132% at 20 mg/L of Corni fructus, respectively. And the adhesion of PMA-chemical stimulated U937 cells to HUVECs, it was inhibited 139% at 0.2 mg/L of Anthrisci radix, 442% at 5 mg/L of Psoraleae semen, 720% at 10 mg/L of Siegesbeckiae herba and 664% at 20 mg/L of Corni fructus. Also, the adhesion of chemical stimulated U937 cells to IL-$1\beta$/chemical stimullated HUVECs, it was inhibited by 286% at 0.2 mg/L of Anthrisci radix, 146% at 5 mg/L of Psoraleae semen, 436% at 10 mg/L of Siegesbeckiae herba and 297% at 20 mg/L of Corni fructus, respectively. It would be a useful substance for anti-cell adhesion based on anti-angiogenesis for anti-obesity and anti-cancer.

• KSBB Journal
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• v.24 no.6
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• pp.549-555
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• 2009

4 kinds of Regional Special Natural Products (RSNPs), such as Anthrisci radix, Psoraleae semen, Siegesbeckiae herba and Corni fructus were examined to verify for anti-obesity effect. $PPAR\gamma$ (peroxisome proliferator-activated receptor $\gamma$) from 3T3-L1 cell concerning adipocyte differentiation was suppressed by different concentraton of 4 RSNPs with western blot, when treated RSNPs' extract and MDI (IBMX, Dexamethasone, Insulin) at the same time. Also, SREBP-1 (Sterol regulatory element binding protein) controlling lipogenesis and $PPAR\gamma$ expression levels were reduced by these 4 RSNPs' extract, when these chemicals after differentiation of 3T3-L1 cell. And lipid droplets were reduced by 7.5%, 14.4%, 18.3% and 30% at different concentration of Anthrisci radix from Oil Red O staining. Also, it was reduced by 2%, 4.9%, 9.3% and 38% at different concentration of Psoraleae semen. For Siegesbeckiae herba, it was inhibited by 1.4%, 6.4%, 16.4% and 30.1%, respectively. And Corni fructus was also showed by 0.9%, 6.3%, 13.7% and 33% at same concentration of Siegesbeckiae herba. These 4 kinds of RSNPs were expected for a useful material for anti-obesity materials.

• Proceedings of the Korean Society of Crop Science Conference
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• 2005.04a
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• pp.302-303
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• 2005

• Korean Journal of Pharmacognosy
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• v.4 no.2
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• pp.95-99
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• 1973

Five steroids $(campesterol,\;stigmasterol,\;{\beta}-sitosterol,\;{\alpha}-spinasterol\;and\;stigmast-7-enol)$ were isolated as mixture from $Siegesbeckia\;pubescens\;M_{AKINO}$ and analyzed by mass spectrometry.

• YAKHAK HOEJI
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• v.16 no.2
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• pp.65-72
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• 1972

A new diterpene glycoside, $C_{26}H_{44}O_{8}{\cdot}H_{2}O, $ m.p. <$225-6^{\circ}, [{\alpha}]_D^{20}=-48^{\circ}$ (0.1% in EtOH) was isolated from methylalcohol extract of Siegesbeckia pubescens $M_{AKINO}$ which has been used as a folk remedy for hypertensive in Korea and its chemical structure was established as (III).

• YAKHAK HOEJI
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• v.24 no.2
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• pp.111-116
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• 1980

This study was performed in dogs and chickens to observe differences of responses by species of animals to hypotensive action of Siegesbeckiae Herba, using its ethanol extract. Siegesbeckiae Herba e ethanol extract (SGEE), when injected into the vein of dogs or chickens, elicited depressor actions in both a animals. The hypotensive effects of SGEE in dogs and chickens were markedly inhibited by the pretreatment of atropine, but not affected by bethanidine, propranolol and diphenhydramine. Phentolamine significantly weakened the action of SGEE in dogs, but did not alter the action of SGEE in chickens. SGEE inhibited the pressor action by norepinephrine or carotid artery occlusion, whereas it did not change the pressor action b by angiotensin in dogs.

• Korean Journal of Pharmacognosy
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• v.4 no.2
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• pp.63-65
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• 1973

A component, $C_{20}H_{32}O_3$, m.p. $207.5^{\circ}$, was isolated from Siegesbeckia pubescens $M_{AKINO}$. This compound was identified as $17-hydroxy-16{\beta}-(-)-kauran-19-oic\;acid$, using spectroscopies and synthesizing various derivatives of the compound.

• The Journal of Korean Medicine
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• v.25 no.1
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• pp.21-30
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• 2004

Objectives : We aimed to identify the effects of Heechum-san(HCS) On the thrombosis. Methods : We measured the protective effects of HCS on pulmonary thromboembolism induced by collagen and epinephrine, intravascular coagulation induced by dextran. Results : HCS showed a effective inhibition on coagulation of platelets induced by ADP or epinephrine. HCS showed survival rate of 14.28% on pulmonary thrombo- embolism caused by collagen and epinephrine. On the measure of the blood flow rate induced by the thrombus, in vivo HCS accelerated the blood flow rate. Conclusions : HCS has antithrombotic effects.