• Title/Summary/Keyword: 희석 효과

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Anti-oxidant and Anti-proliferative Effects of Water Extract Mixture of Cordyceps Militaris and Allium Tuberosum (동충하초 및 부추 혼합 물추출물의 항산화 및 암세포 증식억제 효과)

  • Hong, Seong-Min;Cho, Hyun-Dong;Kim, Jeong-Ho;Lee, Jae-Yoon;Park, Jeong-Mee;Seo, Kwon-Il
    • Journal of Life Science
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    • v.26 no.7
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    • pp.805-811
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    • 2016
  • The present study was performed to evaluate the anti-oxidant and anti-proliferating activity of the water extract mixture of Cordyceps militaris (CM) and Allium tuberosum (AT). The water extract mixture rate of CM and AT was optimized by means of a sensory evaluation test. The optimized mixture rate were decided at 70% of CM, 30% of AT, and 10% of apple concentrate as an additive. The values of total acidity, pH, sugar contents, and turbidity of the water extract mixture were 0.1%, 4.28, 9.10 °Brix, and 1.48 respectively. The water extract mixture had effective DPPH radical scavenging activity, reducing power effect, and ABTS radical activity. DPPH radical activities of the water extract and the water extract mixture were 43.2% and 51.7% respectively; their reducing power (OD700) was 1.14 and 1.43 respectively; and ABTS.+ radical activities were 47.1% and 62.2% respectively. Also, the water extract mixture showed a higher anti-proliferating effect than the AT extract on human prostate cancer cells. These results provided experimental evidence that the water extract mixture of CM and AT is a better source of anti-oxidant and anti-cancer ingredients than a single extract of CM. In conclusion, the water extract mixture of CM and AT will be beneficial in development of a functional drink.

Analysis of breaching behavior of levee according to coating thickness of new substance (신소재의 피복두께에 따른 제방의 붕괴 거동 분석)

  • Ko, Dong Woo;Kim, Sung Joong;Kang, Joon Gu
    • Proceedings of the Korea Water Resources Association Conference
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    • 2018.05a
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    • pp.480-480
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    • 2018
  • 전 세계적으로 자연 친화, 하천생태계 보전, 친수하천 등을 조성하기 위한 대대적인 하천 정비사업이 활발히 진행 중에 있다. 최근 홍수로 인한 제방 붕괴에 대응하기 위한 제방의 안정화 및 개선을 위한 방법으로 기존의 시멘트와 같은 혼합물질을 사용하지 않고 환경 친화적이고 지속 가능한 대안에 대한 수요가 증가되고 있는 추세이며 현재 노후화 된 불안정 제방에 대한 보강대책을 수립해나가는 과정으로써 친환경 신소재를 활용하여 제방을 보호하는 연구가 수행되고 있다. 제방사면에 적용되는 신소재는 바이오폴리머를 활용한 재료로써 공동연구기관 카이스트에서 개발된 환경 친화적인 물질로 미생물에 의해 유도된 고인장 및 인체 무해성 등의 특성을 갖고 있으며 경제적 타당성인 측면에서 시멘트와 비교 분석 되어야 하고 실제 현장에서의 적용 가능성, 신뢰성 및 내구성 검토 등 성능을 보장하기 위한 지속적인 연구가 필요한 상황이다. 이에 본 안동하천실험센터에서는 중규모 제방을 직접 제작하여 수리모형실험을 통한 친환경 신소재 활용 제방의 안정성 및 성능 평가를 실시하였다. 수리실험 조건은 카이스트에서 제시된 레시피를 기반으로 먼저 분말형태의 바이이폴리머를 물과 희석하여 만들어진 바이오폴리머 용액을 흙과 혼합한 뒤 제방표면에 직접 미장작업을 수행하여 실험조건에 따라 일정한 두께(1cm, 3cm, 5cm)로 피복하였다. 이후 월류 붕괴 실험이 가능한 3 - 5일 정도의 양생기간을 거쳐 실험을 진행하였다. 실험결과는 다수의 고프로(GoPro) 및 비디오 카메라 등 다양한 영상장치를 이용하여 픽셀기반의 영상분석기법을 활용한 시간 흐름에 따른 제방 사면에서의 붕괴규모를 산정하여 신소재의 피복 두께에 따른 제체의 붕괴 거동 및 안정성을 평가하였으며, 또한 제방 파괴부에서의 흐름 상황 및 유속이 붕괴 발달에 미치는 영향을 분석하기 위하여 PIV 분석을 실시하였다. 이번 연구의 최종목표는 지속적인 예비실험을 수행하여 월류 및 침투, 파이핑 등 파괴 인자 별 신소재의 성능 개선 및 개발된 새로운 공법에 대한 효과 검토를 통한 최적안을 도출함으로써 향후 실규모 실험실증을 통한 신소재 시공 및 공법에 대한 현장적용 가능성 검증을 거쳐 최종적으로 신소재 제방 공법 설계 기술, 신소재 및 공법 표준안, 제방공법 안정성 평가 가이드라인 등을 제시하고자 하며, 이러한 실험데이터를 축적함으로써 실제 제방 붕괴 시 비상대처계획 수립에 필요한 기초자료로 활용이 가능할 것으로 사료된다.

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Cultivation of Oyster Mushroom Using Non Sterilized Media (무살균 배지를 이용한 느타리버섯 재배)

  • Jang, Hyun-Yu;Kim, Hyuk
    • Korean Journal of Plant Resources
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    • v.14 no.3
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    • pp.196-205
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    • 2001
  • The results of examining cultivation of tlyster mushroom using NSM(Non Sterilized Media) for determining the condition of artificially culturing oyster mushroom(Pleurotus ostreatus) are as follows. Mycelial growth an[1 density of oyster mushroom. were the highest in the medium of waste cotton(spinning) : corn cob(80 : 20, V/V) followed by the order of rice bran, beet pulp. Expecially, mycelial growth and density of oyster mushroom is the lowest at the mixture rate of 80% waste cotton(spinning) : 10% beet pulp. Mycelial growth and density of oyster mushroom. were the highest in the medium of cotton seed hull and beet pulp mixture followed by the order of rice bran, corn cob. Expecially, mycelial growth and density of oyster mushroom is the lowest at the mixture rate of 80% cotton seed hull : above 20% rice bran. Mycelial growth and density of oyster mushroom were the highest in the medium rate of 70% waste cotton(spinning), 10% corn cob and 10% beet pulp(V/V). Mycelial growth and density of oyster mushroom were the highest in the medium rate of 70% cotton seed hull , 10% corn cob and 10% beet pulp(V/V). Optimal concentration of NSM for the mycelial growth and density of oyster mushroom were shown to be 500 times concentration. Optimal water contents for the mycelial growth and density of NSM was 70%.

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A Study on the Fundamental Properties of Mortar Mixed with Converter Slag and Ferronickel Slag (전로슬래그 및 페로니켈슬래그를 혼입한 모르타르의 기초물성 연구)

  • Kim, Ji-Seok;Park, Eon-Sang;Ann, Ki-Yong;Cho, Won-Jung
    • Journal of the Korean Recycled Construction Resources Institute
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    • v.9 no.2
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    • pp.152-160
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    • 2021
  • Converter steel slag(BOF slag) is a vast amount of solid waste generated in the steelmaking process which has very low utilization rate in Korea. Due to the presence of free CaO which can derive bad volume stability in BOF slag, it usually land filled. For recycling BOF and identify its applicability as fine aggregate, this study investigates the fundamental characteristics of mortar with cement replaced ferronickel slag(FNS), which has the potential to be used as a binder. The results suggest that the mineral phases of BOF slag mainly include larnite(CaSiO4), mayenite(Ca12Al14O33) and wuestite(FeO) while olivine crystallines are shown in FNS. The results of flow and setting time reveals that the flowability and process of hardening increased when the amount of FNS and BOF slag incorporated was increased. The length change shows that the amount of change in the length of the mortar was almost constant regardless of mix proportion while compressive strength was reduced. Micro structure test results revealed that FNS or/and BOF slag mix took a long time to react in the cement matrix to form a complete hydration products. To achieve the efficient utilization of B OF slag as construction materials, proper replacement rate is necessary.

Susceptibility of Mutans streptococci in the Planktonic and Biofilm State to Erythrosine (부유 상태와 바이오필름 상태에서 Mutans streptococci의 Erythrosine에 대한 감수성 평가)

  • Gong, Jungeun;Seo, Hyunwoo;Lee, Siyoung;Park, Howon;Lee, Juhyun
    • Journal of the korean academy of Pediatric Dentistry
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    • v.46 no.2
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    • pp.135-138
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    • 2019
  • The aim of this study was to investigate the susceptibility of Mutans streptococci in both planktonic and biofilm states to erythrosine. S. mutans was cultured in brain-heart infusion (BHI) broth. Erythrosine was diluted in BHI broth and prepared at a concentration range of $0.02-10000{\mu}g/L$. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were measured using the microdilution method. After forming biofilms on 96-well plates, the minimum biofilm inhibitory concentration (MBIC) and minimum biofilm eradication concentration (MBEC) were measured. S. mutans was susceptible to erythrosine in both planktonic and biofilm states. MIC and MBC values were both $19.5{\mu}g/L$ for the planktonic state, while MBIC and MBEC values were $313{\mu}g/L$ and $2500{\mu}g/L$, respectively, for the biofilm state. Erythrosine ($19.5{\mu}g/L$) exhibited a bactericidal effect on S. mutans (killing 99.9%) in the planktonic state. For biofilms, erythrosine inhibited biofilm growth and eradicated 99.9% of biofilm bacteria at higher concentrations than MIC and MBC. These MBIC and MBEC concentrations are much lower than known noxious doses, and the MIC, MBC, and MBIC values were even lower than clinical concentrations.

Physico-chemical Properties and Antibacterial Activities of Lactonic Sophorolipid (락톤형 소포로리피드의 물리화학적 특성 및 항균효과)

  • Cho, Soo A;Eom, Gyeong Tae;Jin, Byung Suk
    • Applied Chemistry for Engineering
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    • v.30 no.3
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    • pp.303-307
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    • 2019
  • Sophorolipid is a biological surfactant of the glycolipid structure produced by Candida bombicola, which generally exists as a mixture of acidic and lactonic forms. In this study, we investigated physico-chemical properties, antibacterial activities, and cytotoxicity of the sophorolipid containing more than 96% of the lactonic form, produced by the gene regulation of production strains and application of a metabolic engineering technique. The lactonic sophorolipid showed a weak acidity in the range of pH 3.2~4.6 when diluted in water at the concentrations from 1 to 0.001 wt%. The $pK_a$ value of the lactonic sophorolipid was estimated to be around 4.3 from the acid-base titration curve. The critical micelle concentration (CMC) of the lactonic sophorolipid was $10^{-2}wt%$, at which the surface tension of aqueous solution was reduced to 36 mN/m. The lactonic sophorolipid showed the minimum inhibitory concentrations (MIC) of $1{\times}10^{-3}$ and $5{\times}10^{-3}g/mL$ against Propionibacterium acnes and Corynebacterium xerosis, respectively. The MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide] assay showed that cytotoxicity of the lactonic sophorolipid was ten times lower than that of triclosan.

Removal of COD and Color from Anaerobic Digestion Effluent of Livestock Wastewater by Advanced Oxidation Using Microbubbled Ozone (마이크로버블 오존 고도산화를 이용한 축산폐수 혐기소화 배출수의 COD와 색도의 제거)

  • Lee, Inkyu;Lee, Eunyoung;Lee, Hyejung;Lee, Kisay
    • Applied Chemistry for Engineering
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    • v.22 no.6
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    • pp.617-622
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    • 2011
  • Ozone-based advanced oxidation was applied for the treatment of anaerobic digestion effluent of livestock wastewater. Initial COD and color value were 930 mg/L and 0.04, respectively, and the 1/10-diluted wastewater was used for the study. The treatment characteristics were compared between the conventionally generated ozone ($105{\mu}m$) and microbubbled ozone ($13{\mu}m$). The use of microbubbled ozone improved the removal of chemical oxygen demand (COD) and color by 85% and 26%, respectively, compared with the conventionally bubbled ozone. The application of microbubbled $O_3/UV$, $O_3/H_2O_2$, $O_3/UV/H_2O_2$ combinations resulted in 5~10% higher color removal than ozone alone, which implies that the contribution of UV or $H_2O_2$ is not significant in color removal. On the other hand, COD removal could be increased two folds compared with ozone alone through $O_3/UV/H_2O_2$ combination. The contribution of $H_2O_2$ was bigger than UV for COD removal with microbubbled ozone. Due to the enhancement of dissolved ozone and radical activity, the microbubbling enabled us to additional COD removal even after stopping ozone supply in the presence of UV or $H_2O_2$.

Performance Enhancement of Differential Power Analysis Attack with Signal Companding Methods (신호 압신법을 이용한 차분전력분석 공격성능 향상)

  • Ryoo, Jeong-Choon;Han, Dong-Guk;Kim, Sung-Kyoung;Kim, Hee-Seok;Kim, Tae-Hyun;Lee, Sang-Jin
    • Journal of the Korea Institute of Information Security & Cryptology
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    • v.18 no.2
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    • pp.39-47
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    • 2008
  • Among previous Side Channel Analysis (SCA) methods, Differential Power Analysis (DPA) based on the statistical characteristics of collected signals has been known as an efficient attack for uncovering secret key of cryptosystems. However, the attack performance of this method is affected very much by the temporal misalignment and noise of collected side channel signals. In this paper, we propose a new method to surmount the noise problem in DPA. The performance of the proposed method is then evaluated while analyzing the power consumption signals of Micro-controller chips during a DES operation. Its performance is then compared to that of the original DPA in the time and frequency domains. When we compare the experimental results with respect to the needed number of traces to uncover the secret key, our proposed method shows the performance enhancement 33% in the time domain and 50% in the frequency domain.

Performance Improvement of Power Attacks with Truncated Differential Cryptanalysis (부정차분을 이용한 전력분석 공격의 효율 향상*)

  • Kang, Tae-Sun;Kim, Hee-Seok;Kim, Tae-Hyun;Kim, Jong-Sung;Hong, Seok-Hie
    • Journal of the Korea Institute of Information Security & Cryptology
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    • v.19 no.1
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    • pp.43-51
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    • 2009
  • In 1998, Kocher et al. introduced Differential Power Attack on block ciphers. This attack allows to extract secret key used in cryptographic primitives even if these are executed inside tamper-resistant devices such as smart card. At FSE 2003 and 2004, Akkar and Goubin presented several masking methods, randomizing the first few and last few($3{\sim}4$) rounds of the cipher with independent random masks at each round and thereby disabling power attacks on subsequent inner rounds, to protect iterated block ciphers such as DES against Differential Power Attack. Since then, Handschuh and Preneel have shown how to attack Akkar's masking method using Differential Cryptanalysis. This paper presents how to combine Truncated Differential Cryptanalysis and Power Attack to extract the secret key from intermediate unmasked values and shows how much more efficient our attacks are implemented than the Handschuh-Preneel method in term of reducing the number of required plaintexts, even if some errors of Hamming weights occur when they are measured.

A Study on the Ecocentric Worldview of Elementary School Students (초등학교 4-6학년 학생들의 생태중심 세계관에 대한 연구)

  • Kim, Hee-Seok;Paik, Seoung-Hey
    • Journal of Korean Elementary Science Education
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    • v.40 no.2
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    • pp.227-238
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    • 2021
  • Ecocentric worldview is considered as worldview that overcomes present environment problems and increases animal right. This study explores the formation of an ecocentric worldview for 4th-6th graders based on Peter Singer's research that divides the development of morality into family-centered, species-centered, and ecocentric worldview. The subjects of this study were 77 4th graders, 84 5th graders, and 91 6th graders in Daegu Metro- politan City. The results of surveying students' worldview based on the questionnaire developed by dividing into 6 questions are as follows. In most of the items in grades 4-6, the rate of formation of a species-centered worldview or a family-centered worldview was higher than that of an ecocentric worldview. However, in the case of 6th grade students was significantly higher than in other grades because they learned lesson on wolf restoration in the 5th grade science class. This may be seen as an effect of education, but since the ecocentric worldview did not appear high in other questions, it is reasonable to assume that 6th graders simply recited what they learned rather than forming a true ecocentric worldview. Therefore, there is a need for education that can help students to think, judge, and act on the basis of the ecocentric worldview consistently in any situation, rather than just memorizing the knowledge of the ecocentric worldview learned.