• Title/Summary/Keyword: 흐름 유전 영동

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수직 이중 층이 도입된 나노갭 소자를 이용한 금나노입자 검출

  • Bae, Jin-Yeong;Lee, Cho-Yeon;Park, Dae-Geun;Kim, Su-Hyeon;Pyo, Han-Na;Yun, Wan-Su
    • Proceedings of the Korean Vacuum Society Conference
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    • 2015.08a
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    • pp.231.2-231.2
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    • 2015
  • 본 연구에서는 나노갭 소자에 미세유체 수직 이중층을 도입하여 상층에 존재하는 금나노입자를 검출하였다. 형성된 수직 이중 층의 상층에는 검출물질을 주입하였고 하층에는 검출물질과 소자 표면의 전극을 분리 시킬 수 있는 용액을 주입하였다. 수직 이중층의 형성은 크로노암페로메트리(Chronoamperometry)을 이용하여 상층에 흘려준 electrochemical indicator 인 ferricyanide 용액의 전기화학 신호가 발생되지 않음을 통해서 확인하였다. 연속적인 수직 이중 층의 흐름에서 유전영동법을 이용하여 상층에 존재하는 금나노입자들을 나노갭전극으로 유도포획 하였고 이때 실시간으로 변하는 전류 값으로부터 금나노입자의 검출여부를 판단하였다.

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Deterministic Lateral Displacement as a Function of Particle Size Using a Piecewise Curved Planar Interdigitated Electrode Array (다중예각 평면 교차전극을 이용한 입자 크기에 따른 측면방향 변위)

  • Han, Song-I;Joo, Young-Dong;Han, Ki-Ho
    • Transactions of the Korean Society of Mechanical Engineers B
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    • v.36 no.3
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    • pp.241-249
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    • 2012
  • This paper presents the lateral displacement of a particle passing over a planar interdigitated electrode array at an angle as a function of particle size. The lateral displacement was also measured as a function of the angle between the electrode and the direction of flow. A simplified line charge model was used for numerical estimation of the lateral displacement of fluorescent polystyrene (PS) beads with three different diameters. Using the lateral displacement as a function of particle size, we developed a lateral dielectrophoretic (DEP) microseparator, which enables the continuous discrimination of particles by size. The experiment using an admixture of 3-, 5-, and $10-{\mu}m$ PS beads showed that the lateral DEP microseparator could continuously separate out 99.86% of the $3-{\mu}m$ beads, 98.82% of the $5-{\mu}m$ beads, and 99.69% of the $10-{\mu}m$ beads. The lateral DEP microseparator is thus a practical device for the simultaneous separation of particles according to size from a heterogeneous admixture.

Genetic Diversity and Population Structure of Codium fragile (SURINGAR) HARlOT in Korea Using Allozymes (알로자임을 이용한 청각의 유전적 다양성과 집단구조)

  • Lee Bok-Kyu;Park So-Hye;Heo Youn-Seong;Ju Mu-Teol;Choi Joo-Soo;Huh Man-Kyu
    • Journal of Life Science
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    • v.16 no.2 s.75
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    • pp.213-218
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    • 2006
  • The study of genetic diversity and population structure was carried out in the Codium fragile using allozyme analysis. Although this species has been regarded as a ecologically and economically important source, there is no report on population structure in Korea. Starch gel electrophoresis was used to investigate the allozyme variation and genetic structure of four Korean populations of this species. Of the 15 genetic loci surveyed, nine (60.0%) was polymorphic in at least one population. Genetic diversity was high at the species level ($H_{ES}$=0.144), and, that of the population level was relatively low ($H_{EP}$=0.128). Nearly 87% of the total genetic diversity in C. fragile was apportioned within populations. The predominant asexual reproduction, population fragmentation, low fecundity, geographic isolation and colonization process are proposed as possible factors contributing to low genetic diversity in this species. The indirect estimated of gene flow based on $G_{ST}$ was 1.69. The moderate level of gene flow in C. fragile populations is mainly caused by thallus developed from isolated utricles dispersal via sea current.