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Increased Abiotic Stress Tolerance by Over-expressing OsABF2 in Transgenic Arabidopsis thaliana (OsABF2를 과발현시킨 애기장대에서 비생물학적 스트레스에 대한 내성 증가)

  • Park, Phun Bum
    • Journal of Life Science
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    • v.22 no.11
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    • pp.1515-1522
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    • 2012
  • The phytohormone abscisic acid (ABA) plays an important role in the adaptive response of plants to abiotic stresses. ABA also regulates many important processes, including seed dormancy, germination, inhibition of cell division, and stomatal closure. OsABF2 (Oryza sativa ABRE binding factor2) is one of the bZIP type transcription factors, which are involved in abiotic stress response and ABA signaling in rice. Expression of OsABF2 is induced by ABA and various stress treatments. Findings show that survival rates of OsABF2 over-expressing Arabidopsis lines were increased under drought, salt, and heat stress conditions. The germination ratio of OsABF2 over-expressing Arabidopsis lines was decreased in the presence of ABA. Results indicate that OsABF2 over-expressing Arabidopsis lines have enhanced abiotic stress tolerance and have increased ABA sensitivity.

Physiological Function of Insulin-like Peptides in Insects (곤충 insulin-like peptide의 생리 조절 작용)

  • Kim, Doo Kyung;Lee, Jaemin
    • Korean journal of applied entomology
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    • v.61 no.1
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    • pp.85-90
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    • 2022
  • Insulin and insulin-like growth factor-1 (IGF-1) are hormones that play an important role in the physiological regulation of metabolism, growth, and longevity in vertebrates. Likewise, insulin-like peptides (ILPs), which are structurally similar to insulin and IGF-1, are crucial in insect physiology. In this review, we present an integrated summary of insect ILPs and their receptor signaling, which has been shown to be comparable to insulin and IGF-1 receptor signaling in vertebrates based on genetic studies of Drosophila melanogaster. Additionally, we review the control of ILP synthesis and secretion in the brain in response to nutrition, as well as the ILPs' physiological role in insect metabolism. Moreover, we discuss the contribution of ILPs to growth, development, reproduction, and diapause. Finally, we consider the possibility of targeting ILP receptor signaling in pest management.

Seed Germination and Seedling Emergence of Indian jointvetch (Aeschynomene indica L.) in Different Conditions (자귀풀 종자의 발아 및 출아에 미치는 환경요인)

  • Jin, Chang Hao;Uddin, Md. Romij;Pyon, Jong-Yeong
    • Korean Journal of Weed Science
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    • v.30 no.1
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    • pp.25-33
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    • 2010
  • Several experiments were conducted in growth chambers and a greenhouse to determine the influence of various environmental factors on seed germination and seedling emergence of Indian jointvetch. Fully matured seeds of Indian jointvetch germinated only 42%. The germination percent increased as the storage temperature increased with time. More than 90% seeds germinated when the seeds were kept at $40^{\circ}C$ for seven months, but germination was 58.9 and 55.1% when kept at 25 and $4^{\circ}C$, respectively. Non-dormant seeds of Indian jointvetch germinated 91.1 and 92.4% at 30 and $30/25^{\circ}C$, respectively. Germination percent increased with increasing both prethermal temperature and time. The prethermal temperature of $90^{\circ}C$ for 40 minutes was the best for maximum germination (94.5%). Germination and growth of Indian jointvetch tended to decrease slightly until -0.3 MPa osmotic potential (water stress induction) and then declined drastically and the seeds did not germinate at below -0.5 MPa osmotic potential. Indian jointvetch seems to grow well in moist and flooding conditions since emergence and growth of seedling increased with increasing soil moisture content and the water level.

Anatomy and Artificial Seed Propagation in Anti -cancer Plant Orostachys japonicus A. Berger (항암식물 와송의 해부 형태적 관찰과 대량 증식에 의한 인공재배연구)

  • 신동영;이영만;김학진
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.39 no.2
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    • pp.146-157
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    • 1994
  • Wasong(Orostachys japonicus A. Berger) has been used as anti-cancer medicinal plants from ancient time. This experiment was conducted to obtain some fundamental informations concerning anatomical study, germination test, soil analysis of indigenious districks, adaptaion of soil for wasong. The leaf of wasong was xerotype with spine, mesophyl chloroplast is dense surrounded vascular bundle, with a many large water storage tissue without chloroplast, and was bifacial stomata leaf type. The stem of wasong was amphicribral vascular bundle, central cylinder was cylinder type and protostele. Root was polarch type, potoxylem of it's was arranged in ring shape of annulation and metaxylem. The structure of flower was cymose, was caylyx of 5 sepals, corolla of 5 petals, roecium of 10 stames, ovules of five, placentation parietal, ovary superior, axile placenta. The optimum temperature for seed germination was $25^{\circ}C$ under light and germination percentage was 22.5%. Dormancy breaking was effective for 6-8 days at 5$^{\circ}C$ and at 100 ppm level of $GA_3$ The soil analysis of wasong local districks, content of available $P_2O_5$, Ca were higer than optimal level of upland and C.E.C., exchangeable k was not significantly different of that, but exchangeable magnesium was very low. The growth of wasong was affected draining regardless soil combination treatment.

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Vegetational Composition and flora of Jungnangcheon in Seoul (서울 중랑천의 식생구성과 식물상)

  • 이유미;박수현;정승선
    • Korean Journal of Environment and Ecology
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    • v.16 no.3
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    • pp.271-286
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    • 2002
  • To understand the status of vegetational composition of Jungnangcheon, 21 plots in 7 sites were investigated in June 2001 and flora survey was done during the period from June to September 2001. The results obtained from this study were as follows: Vegetational compositions of 7 sites in Jungnangcheon area were different site by site and plot by plot. In lower(nearest) point, Phalaris arundinacea, Potentilla supina, Rorippa islandica, Chenopodium album, Beckmannia syzigachne and Chenopodium ficifolium showed higher constancy and frequency. Bromus japonicus, Humulus japonicus, Conyza canadensis, Elymus tsukusiensis var. transiens showed higher constancy and frequency in middle point and Bromus japonicus, Chenopodium ficifolium, Artemisia princeps var. orientalis showed in upper point. Bromus japonicus, Chenopodium ficifolium, Humulus japonicus, Conyza canadensis, Elymus tsukusiensis var. transiens, Artemisia princeps var. orientalis and Lepidium uirginicum appeared at more than 9 polts in 21 sample plots. Number of naturalized plants in Jungnangcheon area were 79 taxa. Total naturalized index(NI) and urbanized index(UI) were recorded as 29.2%, 27.9%. 253 taxa including 50 families 169 genera 224 species 1 subspecies 27 varieties 1 forma were recorded in Jungnangcheon area. Therophyte(Th) was the most common dormancy form recorded in survey area. Geophyte(G) and Hemicryptophyte(H) were calculated as 7.1%, 17.8% and it was the lower value compared to those of other dormancy form. Consequently, Th-R$_{5}$-D$_4$-e was recorded as the most common life form in Jungnangcheon.

Update Protocols for Web-Based GIS Applications (웹 기반 GIS 응용을 위한 변경 프로토콜)

  • An, Seong-U;Seo, Yeong-Deok;Kim, Jin-Deok;Hong, Bong-Hui
    • Journal of KIISE:Databases
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    • v.29 no.4
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    • pp.321-333
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    • 2002
  • As web-based services are becoming more and more popular, concurrent updates of spatial data should be possible in the web-based environments in order to use the various services. Web-based GIS applications are characterized by large quantity of data providing and these data should be continuously updated according to various user's requirements. Faced with such an enormous data providing system, it is inefficient for a server to do all of the works of updating spatial data requested by clients. Besides, the HTTP protocol used in the web environment is established under the assumption of 'Connectionless'and 'Stateless'. Lots of problems may occur if the scheme of transaction processing based on the LAN environment is directly applied to the web environment. Especially for long transactions of updating spatial data, it is very difficult to control the concurrency among clients and to keep the consistency of the server data. This paper proposes a solution of keeping consistency during updating directly spatial data in the client-side by resolving the Dormancy Region Lock problem caused by the 'Connectionless'and 'Stateless'feature of the HTTP protocol. The RX(Region-eXclusive) lock and the periodically sending of ALIVE_CLIENTi messages can solve this problem. The protocol designed here is verified as effective enough through implementing in the main memory spatial database system, called CyberMap.

A Centralized Deployment Protocol with Sufficient Coverage and Connectivity Guarantee for WSNs (무선 센서 네트워크에서 유효 커버리지 및 접속성 보장을 위한 중앙 집중형 배치 프로토콜)

  • Kim, Hyun-Tae;Zhang, Gui-Ping;Kim, Hyoung-Jin;Joo, Young-Hoon;Ra, In-Ho
    • Journal of the Korean Institute of Intelligent Systems
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    • v.16 no.6
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    • pp.683-690
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    • 2006
  • Reducing power consumption to extend network lifetime is one of the most important challenges in designing wireless sensor networks. One promising approach to conserving system energy is to keep only a minimal number of sensors active and put others into low-powered sleep mode, while the active sensors can maintain a connected covet set for the target area. The problem of computing such minimum working sensor set is NP-hard. In this paper, a centralized Voronoi tessellation (CVT) based approximate algorithm is proposed to construct the near optimal cover set. When sensor's communication radius is at least twice of its sensing radius, the covet set is connected at the same time; In case of sensor's communication radius is smaller than twice of its sensing radius, a connection scheme is proposed to calculate the assistant nodes needed for constructing the connectivity of the cover set. Finally, the performance of the proposed algorithm is evaluated through theoretical analysis and extensive numerical experiments. Experimental results show that the proposed algorithm outperforms the greedy algorithm in terms of the runtime and the size of the constructed connected cover set.

Feed Evaluation, Germination and Early Growth of Puccinellia coreensis Honda and P. nipponica Ohwi as affected by Salt Concentration (갯겨이삭, 갯꾸러미풀의 조사료 평가와 발아 생리 및 염농도별 초기생육)

  • Kim, Young-Doo;Park, Tae-Ill;Chae, Jae-Suk;Chang, Young-Sun;Park, Keun-Yong
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.40 no.3
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    • pp.269-277
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    • 1995
  • This experiment was conducted to study on forage utility of native grasses in polderland for salt tolerance plant selection and good feed with collection and evaluation. As the result of investgate, it was carried out feed evaluation, germination physiology and early growth at the different salt content of Puccinellia coreesis Honda and P. nipponica Ohwi from May 1993 to June 1994. Germination physiology of the grasses was light and low temperature favored optimum germination temperature was 1$0^{\circ}C$, germination period was 30 days. Dormancy awakening was needed wet and high temperature condition with 1~3 days storage. Germination rate increased 18.0% of P. coreensis and 39.0% of P. nipponica than those of non treatments. Early growth condition of different salt content was good also at in 1.00% and 0.50% levels of P. coreensis and P. nipponica. Crude protein content was 8.4% at P. coreensis, 7.1% at P. nipponica and 10.3% of Italian ryegrass, and NFE content was arrangement in order of P. coreensis, Italian ryegrass and P. nipponica. TDN content was not different between P. coreensis, P. nipponica and Italian ryegrass. Also P. coreensis and P. nipponica have a good taste for cattle as same as other crops.

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Studies on the Regional Characteristics in Occurrence of the Rice Stem Borer, Chilo suppressalis Walker in Korea (한국에서 이화명나방(Chilo suppressalis Walker) 발생상의 지역적 특성에 관한 연구)

  • 박창규;현재선
    • Korean journal of applied entomology
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    • v.29 no.4
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    • pp.257-268
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    • 1990
  • The regional characteristics in the occurrence of the rice stem borer, Chilo suppressalis (Walker), in Korea, were examined by analyzing the light trap data obtained from 39 locations during the years of 1965-1988, and some ecological significance of the overwintering larval populations collected from the four locations where some of the typical ecological-race have been known, the results obtained are as follows; The total number of the moths have decreased greatly throughout the nation since 1970. The decrease in the total number of moths related with those in the spring moth but not in the summer moths, and suggested that the changes in the rice production systems have effected significantly on the second generation of the insect. The variations between the regions had been gret in 1960's, decreasing phase in 1970's and increasing phase in loast decade. The overall average of 50% emergence dates have shifted earlier in both moths, and the changes were different considerably with the regions as wheel as in generations. When the overwintered larvae were incubated at $25^{\circ}C$ and RH of 90%, the average mortalities were 21.07% for Suweon, 43.06% for Iri, 63.51% for Jinju, 27.04% for Gwangju. When the overwintered larvae were incubated at $25^{\circ}C$ and RH of 90%, the average mortalities were different with the body weight; 45.44% for the larvae of less than 39 mg, 37.61% for 40-49 mg, 37.28% for 50-59 mg, 30.17% for greater than 60 mg. When the overwintered larvae wee incubated at $25^{\circ}C$, post-diapause larval development periods of body weight classes were 18.6$\pm$1.2 days for the larvae of less than 39 mg, 20.4$\pm$3.3 days for 40-49 mg, 21.4$\pm$3.2 days for 50-59 mg, 21.8$\pm$3.2 days for grater than 60 mg, and only the larvae of less than 39 mg, body weight class less than 39 mg was found statistically different in post-diapause larval development periods. From these results, the changes in the cultural practice of the rice have effected on the ecological pattern of the rice stem borer, and the effects seemed to be different with the generations, and with the regions where the different ecological races are distributing.

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Studies on the Induction of Sprouting of Dormant Seed Potato in Fall Crop Production (추작감자의 최아법에 관한 연구)

  • Jae-Young Cho
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.21 no.1
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    • pp.97-124
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    • 1976
  • To find out the most effective method of treatments for the induction of sprouting of dormant seed potato pieces for the fall crop production of Irish cobbler, this experiment was carried out with sprout inducing bed and field performance trial. In GA treatments, about 10 days were required to sprout and resulted uniform and thin 3-4 sprouts per tuber piece, but sprouts were slender and rooting was not observed. In Ethrel treatments, 20-25 days were required, and sprouts were inferior than that of GA treatment in uniformity of sprouting, and percentage of rotten pieces and of healthy sprouted tuber pieces, but number of sprouts per tuber pieces was low, being 1-2, and sprouts were short, thick, and healthy, and showed good rooting. In GA and Ethrel mixture treatments, 1-2 more days were required to sprout than GA treatments, but sprouts were relatively healthy, and other sprouting pattern were like that of GA treatments. In Ethylene chlorohydrin and 6-Benzyl-adenine treatments, sprouting was like that of Ethrel treatments, but much more days were required than Ethrel treatments and tendency of severe rotting was observed. Optimum treating methods of promising chemicals found to be 1-2 and 2-5 ppm GA solution, 500 and 1000-2000 ppm Ethrel solution, and 1-2+250-500 and 5+250-500 ppm GA and Ethrel mixture solution for 60 min. treatment of tuber piece and whole-tuber, respectively. Induction of sprouting in dry and hot time resulted severer rotting of tuber pieces during the induction of sprouting and with the advancement of dormancy, being delayed in date of treatment, tendency of promotion of sprouting and rooting was observed. When sprouted tuber piece was transplanted at the same date, yields were in order of Ethrel, GA and Ethrel mixture, and GA treatment, indicating the correlation between yield and healthiness of sprout and rooting status of sprouted tuber piece. In all treatments, earlier transplanting resulted higher yields.

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