• Journal of the Korean Chemical Society
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• v.50 no.4
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• pp.298-306
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• 2006

A rapid, simple and sensitive validated spectrophotometric methods have been described for the assay of neomycin and streptomycin either in pure form or in pharmaceutical formulations. The proposed methods were based on the oxidation of the studied drugs by a known excess of potassium permanganate in acidic medium and estimating the unreacted permanganate with amaranth dye (method A), acid orange II (method B), indigocarmine (method C), and methylene blue (method D), in the same acid medium at a suitable lmax=521, 485, 610 and 664 nm, respectively. Beers law is obeyed in the concentration range of 5-10 and 2-7 mg mL-1 for neomycin and streptomycin, respectively. The apparent molar absorptivity and sandell sensitivity values are in the range 5.47-6.20104, 2.35-2.91105 L mol-1 cm-1 and 7.57-8.59, 5.01-6.2 ng cm-2 for neomycin and streptomycin, respectively. Different variables affecting the reaction were studied and optimized. The proposed methods were applied successfully to the determination of the examined drugs either in a pure or pharmaceutical dosage forms with good accuracy and precision. No interferences were observed from excipients and the results obtained were in good agreement with those obtained using the official methods.

• Journal of the Korean Society of Food Science and Nutrition
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• v.34 no.3
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• pp.361-365
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• 2005

For the EXP 1, the objective of this study was to determine the effect of MSM (methyl sulfonyl methane) supplementation on growth performance and nutrient digestibility in pigs. Sixty crossbred (Landrace × Yorkshire × Duroc) pigs (48.15±0.15㎏ average initial body weight) were used in a 35 days growth assay. Dietary treatments included 1) Control (basal diet), 2) T1 (basal diet＋0.01% MSM) and 3) T2 (basal diet＋0.02% MSM). For overall period, average daily gain, average daily feed intake and feed efficiency were not significantly different among the treatments (p＆gt;0.05). Digestibilies of DM, N, Ca and P were not significant defferences (p＆gt;0.05). For the EXP 2, the objective of this study was to determine the effect of MSM and antibiotic supplementation on growth performance and nutrient digestibility in pigs. One hundred crossbred (Landrace × Yorkshire × Duroc) pigs (33.85±0.15㎏ average initial body weight) were used in a 42 days growth assay. Dietary treatments included 1) Control (basal diet), 2) T1 (basal diet＋0.05% neomycin sulfate, 0.055% oxytetracycline), 3) T2 (Con diet＋0.01% MSM) and T3 (T1＋0.01% MSM). For overall period, average daily gain and average daily feed intake of pigs fed T3, T2 diets were higher than those of CON diet (p＆lt;0.05). Average daily gain was not significantly different between T2 and T3. However, food efficiency of pigs fed CON was the highest among the treatments (p＆lt;0.05). Pigs fed T3 diet increased nutrient digestibility compared to other treatments (p＆lt;0.05). In conclusion, the results suggest that the dietary addition of MSM and antibiotics into diets for pigs affect growth performance and nutrient digestibility.