• Korean Journal of Food Science and Technology
• /
• v.29 no.2
• /
• pp.194-197
• /
• 1997

Dopamine ${\beta}-hydroxylase\;(DBH)$ catalyses the enzymatic reaction of dopamine to norepinephrine. For the purpose of screening DBH inhibitory activity from edible mushrooms, Ganoderma lucidum, Agaricus bisporus and Lentinus edodes were examined by tracing inhibitory activities against bovine adrenal DBH, utilizing tyramine as a substrate. Among the three edible mushrooms tested, Ganoderma lucidum showed potent enzyme inhibitory activilies above 100% against DBH in chloroform fraction. Lentinus edodes and Agaricus bisporus showed inhibitory activities in ethylacetate fraction on 79.7% and 64.7%, respectively. Each solvent fraction of these mushrooms were assessed in the aspects of their inhibitory activities against DBH, and their $IC_{50}$ values were calculated. $IC_{50}$ value of chloroform fraction of Ganoderma lucidum was $1.60{\times}10^{-4}\;g$, and those of ethylacetate fractions of Agaricus bisporus and Lentinus edodes were $5.50{\times}10^{-4}\;g\;and\;2.35{\times}10^{-4}\;g$, respectively.

• Applied Biological Chemistry
• /
• v.48 no.3
• /
• pp.212-216
• /
• 2005

Thapsigargin is a specific antagonist of SR/ER-type $Ca^{2+}-ATPase$ in animal tissue, and it was used to characterize the microsomal ATPases prepared from the roots of tomato. When $10\;{\mu}M$ thapsigargin was added, it inhibited the microsomal ATPase activity by 30%. The thapsigargin-induced inhibition was dose-dependent. Since the activity of $Ca^{2+}-ATPase$ is very low in the roots of tomato tissue, it is possible that thapsigargin inhibits the activities of major $H^+-ATPases$ located in plasma and vacuolar membranes. The inhibitory effect of thapsigargin was reduced when the vacuolar $H^+-ATPase$ activity was inhibited by ${NO_3}^-$. However, the effect of thapsigargin was not observed on the $H^+-ATPase$ activity located in the plasma membrane. These results suggest that thapsigargin inhibits the vacuolar $H^+-ATPase$ activity in the roots of tomato.

• Proceedings of the Korean Society of Postharvest Science and Technology of Agricultural Products Conference
• /
• 2003.04a
• /
• pp.97.1-97
• /
• 2003

깻잎중의 각종 성인병 관련 생리기능성을 탐색하여 고부가가치의 기능성 제품을 개발하고자 먼저 1월, 5월 시설재배 깻잎과 8월 노지재배 깻잎의 extracts 수율을 조사하였다. 8월 노지에서 재배한 깻잎의 알콜 extracts 수율이 29%로 제일 높았고 1월과 5월 시설재배 깻잎보다는 8월 노지재배 깻잎의 extracts수율이 더 높았다. 물과 30% 알콜 깻잎 추출액에 대한 생리기능성으로 혈전용해활성은 5월 시설재배 깻잎의 알콜 추출물이 8.2 U로 제일 높았고 안지오텐신 전환효소(ACE) 저해활성은 1월 시설재배 깻잎의 물 추출물에서 64.5%의 높은 활성을 보여 고혈압 예방(치료) 제품개발에 매우 유용할 것으로 생각된다. 항산화활성은 물 추출물보다는 대체로 30% 알콜 추출물에서 높았고, 특히 8월 노지재배 깻잎의 알콜 추출물에서 69%을 보였다. 고지혈증 예방(치료)에 관련된 HMG-Co A reductase 저해활성은 8월 노지재배의 물 추출물에서 83%의 높은 활성을 보였고 elastase 저해활성은 8월 노지재배 깻잎의 30% 알콜 추출물에서 47.5%의 활성을 보였다. 아질산염 제거활성과 SOD유사활성 및 tyrosinase 저해활성 등은 없거나 매우 미약하였다.

• Korean Journal of Food Science and Technology
• /
• v.46 no.1
• /
• pp.44-50
• /
• 2014

We evaluated the enzyme inhibitory activity of germinated rough rice (Oryza sativar L.) treated by high pressure (30MPa) for 24 h (HP24) and 48 h (HP48). In rice germinated for 1 day, the ${\alpha}$-glucosidase inhibitory activity reached its highest level, 68.32%, at HP48. The ${\alpha}$-amylase inhibitory activity increased from 32.66-57.00% at HP0, to 43.67-74.82% at HP48. On the other hand, the inhibitory activity of angiotensin-converting enzyme increased from 27.98% to 49.42% over the course of the second day of HP48. The inhibitory activity of xanthine oxidase peaked of 67.51% at HP48 and subsequently decreased. Lipase inhibitory activity increased from 24.04-47.91% at HP0, to 29.62-64.63% at HP48. These results provide useful information for the use of germinated rough rice as a functional food material and demonstrate that high-pressure treatment during the germination process efficiently increase enzyme inhibitory activity.

• Journal of the Korean Society of Food Science and Nutrition
• /
• v.44 no.7
• /
• pp.993-999
• /
• 2015

For the purpose of investigating the in vitro antidiabetic activity of a medicinal herb and herb mixture extracts prepared through traditional antidiabetic prescription, this study examined ${\alpha}$-glucosidase inhibitory activity. Tyrosinase, a type I membrane glycoprotein, is synthesized and glycosylated in the endoplasmic reticulum (ER) and Golgi. The enzyme is subsequently transported to melanosomes, where it participates in melanogenesis. Previous studies showed that disruption of early ER N-glycan processing by an ${\alpha}$-glucosidase inhibitor suppresses tyrosinase enzymatic activity and melanogenesis. According to the results, most oriental medicinal herbal extracts were stronger than acarbose and N-butyldeoxynojirimycin, known as an ${\alpha}$-glucosidase inhibitor. Interestingly, ethyl acetate layer of enzyme hydrolyzed Cheongsimyeonjaeum had an inhibitory effect on melanin synthesis in B16F1 cells, although it did not inhibit tyrosinase activity directly. Together, ${\alpha}$-glucosidase inhibition activity could be used to evaluate anti-melanogenesis, although cross-checking with melanin inhibitory assay is recommended.

• Journal of the Korean Society of Food Science and Nutrition
• /
• v.36 no.9
• /
• pp.1113-1119
• /
• 2007

Water extracts from root, stem and leaf of Ailanthus altissima were utilized to determine antioxidant properties such as electron donating ability (EDA), nitrite scavenging ability, superoxide dismutase (SOD)-like activity, and the inhibitory activities of xanthine oxidase (XO) and tyrosinase. The EDA of root extract was the highest as 77.33% at 0.5 mg/mL concentration and that of stem extract was 70.01% at 1.0 mg/mL. The nitrite scavenging ability of leaf extract revealed the highest effect as 95.18% at pH 1.2, 1.0 mg/mL while those of stem and root extracts were 55.17% and 33.33%, respectively. The leaf extract showed the highest SOD like activity as 26.77% at 1.0 mg/mL, the measurement of root extract was 3.82% and that of stem extract was not effective. All kinds of extracts had strong inhibitory activities on XO of over 92% at 1.0 mg/mL. The highest activity on tyrosinase inhibition was obtained from leaf extract of 16.33% at 2.0 mg/mL. The results indicated that among the three extracts, the leaf extract has a strong and extensive antioxidant activity.

• Journal of the Korean Society of Food Science and Nutrition
• /
• v.39 no.11
• /
• pp.1573-1579
• /
• 2010

This research was performed to determine the antioxidant activity, nitrite scavenging activity, and its inhibitory activity on angiotensin converting enzyme (ACE), xanthine oxidase, $\alpha$-glucosidase, and elastase of hot water extract from pine bud (WPB). Antioxidant activity of WPB was measured by 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity and superoxide dismutase (SOD)-like activity. DPPH radical scavenging activity and SOD-like activity of WPB were remarkably increased in a dose-dependent manner, and were about 71.4 and 85.4% at 2 mg/mL, respectively. The xanthine oxidase and ACE inhibitory activities were about 70.9 and 51.9% at 2 mg/mL of WPB, respectively. Nitrite scavenging activity of WPB was about 59.1, 53.8, and 39.5% on pH 1.2, 3.0, and 6.0 at 2 mg/mL, respectively. The WPB also showed elastase and $\alpha$-glucosidase inhibitory effects. These results revealed that pine bud have strong antioxidant activity and positive effects on the inhibition of xanthine oxidase, ACE, and elastase.

• Applied Biological Chemistry
• /
• v.43 no.2
• /
• pp.95-99
• /
• 2000

A series of hetero ring (X) substitued chalcone derivatives with farnesyl protein transferase (FPTase) inhibition activities $(pI_{50})$ values determined in vitro is analyzed by modified Free-Wilson (F-W) and Hansch method for quantitative structure activity relationship (QSARs). On the basis of F-W analysis on the FPTase inhibitory activity of a training set of the compounds, none of the (X)-substituents were not contribute the activity. But the net charge of ${\alpha}$ carbon atom is contribute the activity than that of ${\beta}$ carbon atom. And the relative orders of the (Y)-substituents on the activity are ortho>meta>para-substituents. According to Hansch approach, the activities would depend largely on the optimal, $(R_{opt.}=-0.35)$ resonance effect with ortho substituted $(I_o>0)$ electron donating group (R<0) and STERIMOL parameter, $B_1$ constant. The inhibition activity between hetro ring substituents have been a proportioned with each others and none substituent(H), 45 showed the highest FPTase inhibition $(pI_{50}=4.30)$ activity.

• The Korean Journal of Mycology
• /
• v.43 no.2
• /
• pp.99-103
• /
• 2015

We prepared supernatants and cell-free extracts of yeasts isolated from wild flowers in Korea, and their antioxidant activity and inhibitory activities of xanthine oxidase and tyrosinase were investigated. Among them, cell-free extracts of Kuraishia capsulata UL40-2 and Sporobolomyces ruberrimus 121-Z-3 showed significantly high xanthine oxidase inhibitory activity of 46.4% and 48.3%, respectively. Starmerella bombicola 80-J-1 also showed tyrosinase inhibitory activity of 36.2% in the cell-free extract. Other antioxidant activities and tyrosinase inhibitory activities were not detected or were below 20%. Maximal production of the xanthine oxidase inhibitors were observed when Kuraishia capsulate UL40-2 and Sporobolomyces ruberrimus 121-Z-3 were cultured in the yeast extract-peptone-dextrose media at $30^{\circ}C$ for 24 hour, respectively.

• The Korean Journal of Mycology
• /
• v.49 no.2
• /
• pp.243-248
• /
• 2021

In this study, screening of potent non-pathogenic wild yeast with high anti-dementia butyrylcholinesterase (BChE) inhibitory activity and production condition of a BChE inhibitor were described. Among 36 non-pathogenic wild yeasts, Saccharomyces cerevisiae WJSL 0113 showed the highest BChE inhibitory activity of 85.2%. The specific BChE inhibitor was maximally produced when S. cerevisiae WJSL 0113 was cultured at 30℃ for 48 h in a yeast extract-peptone-dextrose medium.