• Proceedings of the KAIS Fall Conference
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• 2012.05a
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• pp.239-242
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• 2012

효모 (Saccharomyces Cervisiae)는 단일 세포의 형태로 존재하는 진핵 세포로써 동물세포와 유사한 기작으로 분비성 단백질을 생성한다. 따라서 박테리아와 달리 효모를 이용하면 당단백질이나 이황결합을 포함하는 분비성 단백질을 경제적으로 대량 합성할 수 있다. 효모의 필수 단백질 중 하나인 단백질 이황이성질화 효소는 소포체에 위치하며 분비성 단백질에 구조적으로 안정한 이황결합을 제공하는 효소이다. 본 연구는 단백질 이황이성질화 효소 (protein disulfide isomerase)가 지니고 있는 두 개의 활성도메인 중 분비성 단백질들의 합성 효율에 직접적으로 관여하는 부위를 찾는 연구이다. 효모 유전체로부터 단백질 이황이성질화 효소의 유전자 (PDI1)을 제거하고 효소의 변이 유전자를 주입한 후 효모의 성장 속도를 측정하였다. 또한 효모의 대표적 분비성 단백질을 각 변이 효소를 지니는 효모에 과발현시켜 합성 및 이황결합 형성 효율을 측정하였다. 단백질 이황이성질화 효소내 두 개의 활성 부위 중 아미노 말단쪽에 위치한 a 도메인에 있는 활성 부위가 분비성 단백질의 활성에 중요한 역할을 한다는 것을 알 수 있었다. 이 결과는 이황결합이나 당을 포함하는 외래 단백질의 고효율 합성을 위한 새로운 효모종 개발에 중요한 정보를 제공할 것으로 기대 된다.

• Korean Journal of Poultry Science
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• v.15 no.4
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• pp.277-280
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• 1988

As a whole, the improvement of the weight gain ratio has rather a clear effect for the groups fed live yeast especially at 0.1% level, comparing with the control, and the nutrient digestibility is also improved, noticeably in protein digestibility. Feed intake is not, however, increased for the live yeast fed groups comparing with the control. The live yeast supplements are apparently responsible for the overall improvement in the nutrient availability and this, in turn, resulted in the weight gain of treated groups over the control.

• Korean Journal of Poultry Science
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• v.18 no.3
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• pp.167-181
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• 1991

In order to study the effects of dietary yeast (Saccharomyces cerevisiae) products on the performance of broiler chickens, tow feeding trial using Arbor Acres strain were conducted. In experiment 1, 200 hatched male broiler chickens were divided into groups of 10 birds each and four groups were given each of the five dietary treatments : control, 0.25% yeast culture supplemented (YC-0.25%), 2.5% yeast culture supplemented(YC-2.5%), 0.05% live yeast supplemented(LY-0.05%) and 0.1% live yeast supplemented(LY-0.1%) . In experiment 2, 240 hatched broiler chickens(120 birds in each sex) were assigned to 2$\times$3(sex$\times$feed) factorial design Dietary treatments were control, 0.1% live yeast supplementation in finisher diet(LY-Finisher) , and 0.1% live yeast supplementation in whole period (LY-Whole). Results of experiment 1 showed that weight gain, feed intake and mortality were not significantly different among treatments. However, weight .gain of YC-0.25% and LY-0.1% tended to be greater than other treatments after 3wks of age Feed efficiency of LY-0.05% was poorer than those of control, YC-0.25% and LY-0.1% . Although nutrients availabilities were not significantly different among treatments, availiabilities of Ca and P were greater in yeast products supplemented groups than in control group. The number of Lactobacillus spp., Streptococcus spp . and yeast in small intestine tended to be greater in supplemented groups while that of Coliforms bacteria tended to be greafter in control group. In experiment 2, there were significant effects of factors (feed and sex) and interaction on growth rate. LY-Whole groups showed best weight gain in male while LY-Finisher groups did best in female broiler chickens. Feed intake and mortality were significantly higher in male broiler chickens. Feed$\times$sex interaction had a significant effect on feed efficiency. LY-Whole groups showed best feed efficiency in male while LY-Finisher did best in female broiler chickens. Availabilities of dry matter, Ca and P were higher in male than in female broiler chickens. Availabilities of Ca and P were higher in live yeast supplemented groups than in control groups. Live yeast supplemented groups tended to have greater number of Lactobacillus spp . and yeast in the small intestine and Stre))tococcu spp. in the small intestine and cecum, and lesser number of Coliforms bacteria in the small intestine The pH of small intestinal contents tended to be higher in live yeast supplemented groups.

• Journal of the Korean Society of Food Science and Nutrition
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• v.46 no.3
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• pp.327-334
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• 2017

Water and 75% ethanol extracts were prepared from defatted green tea seeds and evaluated for their anti-yeast activities. The antimicrobial activities of defatted green tea seed extracts (DGTSEs) were tested against food-spoilage bacteria, yeasts, and molds. DGTSEs exhibited antimicrobial activities with minimum inhibitory concentrations of $39{\sim}1,250{\mu}g/mL$ against three bacteria, two molds, and all tested yeast strains. Ethanol extract showed higher antimicrobial activity than water extract. The stability of anti-yeast activities of DGTSEs was examined under different conditions of temperature, pH, and NaCl concentrations. The anti-yeast activities of DGTSEs were stable at pH 3~9, 0~20% NaCl, and $100^{\circ}C$ for 30 min. However, anti-yeast activities of DGTSEs decreased upon heating at $70^{\circ}C$ for 24 h or $121^{\circ}C$ for 15 min. DGTSEs were applied to food models to determine their inhibitory effects on yeast film formation. Water and 75% ethanol extracts were effective in preventing yeast film formation at concentrations more than 156 and $39{\mu}g/mL$ in soy sauce, 156 and $78{\mu}g/mL$ in pickle sauce, and 78 and $39{\mu}g/mL$ in kimchi, respectively.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.43 no.3
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• pp.265-271
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• 2017

In this study, anti-oxidant, whitening and anti-aging effects were investigated the possibility of fermented black tea yeast as a functional cosmetic material. The results of Aureobasidium pullulans (A. pullulans) ferment of black tea (AFBT) were compared with black tea (BT) and A. pullulans ferment (AF). The antioxidant activity of the AFBT product showed DPPH radical scavenging ability higher than that of AF. In the intracellular ROS experiments induced by hydrogen peroxide, the AFBT showed significant decreased activity. In addition, AFBT showed tyrosinase inhibitory activity and inhibited the formation of melanin using Melan-a and B16F10 cells. Through suppression of MMP-1 expression, the AFBT showed higher anti-wrinkle activity than BT and AF. From the results of this study, it was confirmed that AFBT showed antioxidant activity and was developed as a functional cosmetic material based on whitening and anti-wrinkle efficacies.

• Journal of the Korean Society of Food Science and Nutrition
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• v.45 no.9
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• pp.1351-1357
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• 2016

Green tea (Camellia sinensis) seed extract (GTSE) was prepared under various pretreatment conditions and used to investigate its extraction yield and anti-yeast activity. Anti-yeast activity of GTSE from seeds with or without the coats was the same, whereas the extraction yield was slightly higher in extract from seeds without the coat. Anti-yeast activity of GTSE from seeds with different water contents or particle sizes was the same, whereas the extraction yield was highest in extract from seeds with 7.3% moisture or a smaller particle size. Anti-yeast activity of defatted green tea seed extract (DGTSE) was the same as that of GTSE. Extraction yield was higher in DGTSE from defatted seeds by the oil press machine compared to hexane extraction. Defatted green tea seed (DGTS), a by-product from the oil extraction process, is a good natural source of anti-yeast preservative. The extraction yield and anti-yeast activity of DGTSE were investigated using various extraction solvents, temperatures, and times. The results show that water was an economic extraction solvent, and anti-yeast activity of DGTSE was unstable at $90^{\circ}C$. These results suggest that water, extraction temperature of $50^{\circ}C$, and extraction time of 4 h were the most efficient for extraction of anti-yeast compounds from DGTS.

• KSBB Journal
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• v.19 no.4
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• pp.291-294
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• 2004

The aim of this study was to figure out the antibacterial pattern of leucocin A transformed yeast with culture. Dry cell weight, total secreted protein, and antibacterial activity were increased to 12 hour, after then they showed decrease while protease activity represented the opposite pattern. This implied the production of leucocin A was growth-related. Compared to the result of one hour culture broth, antibacterial activity was about 3.24 fold at 12 hour culture. Maximum growth inhibition rate was 70.57% compared to nontransformed yeast. As the increase of protease in the supernatant, the antibacterial activity was diminished. This study could permit the mass production of bacteriocin to use as antibiotics or food preservatives.

• 대한방사선방어학회:학술대회논문집
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• 2011.11a
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• pp.192-193
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• 2011

• The Korean Journal of Mycology
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• v.51 no.1
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• pp.39-49
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• 2023

This study aimed to screen and investigate the microbiological characteristics, enzyme activity, and physiological functionality of unrecorded wild yeasts from the Wolsung valley of South Deogyu mountain and Jinjamcheon in Daejeon city, Korea. Candida sorboxylosa WSC25-4 (NNIBRFG47336), Arxula adeninivorans WSC6-3 (NNIBRFG47335), Farysizyma taiwaniana JRC6-2 (NNIBRFG47339), Saturnispora dispora JTS19-1 (NNIBRFG47334), Vanderwaltozyma polyspora JJCH5-3 (NNIBRFG47337), and Vanrija fragicola SW-9 (NNIBRFG47338) have not been previously recorded. None of these formed spores or pseudo-mycelium, and four strains, including C. sorboxylosa WSC25-4, were global in shaped. Except for V. polyspora JJCH5-3, all strains grew well in the yeast extract-peptone-dextrose (YPD), potato-dextrose and yeast malt extract media. A. adeninivorans WSC6-3 and S. dispora JTS19-1 grew well in 40% glucose-containing YPD medium and V. fragicola SW-9 in 5% NaCl-containing YPD medium. The supernatant of A. adeninivorans WSC6-3, F. taiwaniana JRC6-2, and V. fragicola SW-9 exhibited 58.0, 59.0, and 59.0% of glucoamylase activity, respectively. C. sorboxylosa WSC25-4 also produced extracellular alkaline protease. Antioxidant activity of the supernatant from F. taiwanian JRC6-2 was high (79.8%), while the other five unrecorded yeasts demonstrated elevated antiaging superoxide dismutase (SOD)-like activity (99%). Anti-gout xanthin oxidase inhibitory activity of S. dispora JTS19-1 and V. fragicola SW-9 was also elevated at 79 and 80%, respectively.

• The Microorganisms and Industry
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• v.19 no.4
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• pp.62-67
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• 1993

의약품을 중심으로 한 광학활성 생리활성물질의 제조 즉, 정밀유기합성은 정밀화학의 꽃이라 할 수 있다. 이러한 광학활성물질은 통상의 유기화학적 수법으로 합성하면 라세미체로 얻어지고 광학활성체로 얻을 수 없다. 그래서 최근 chiral 중심을 가지는 간단한 화합물(광학활성합성원료-chiral building block, chiral synthon)을 원료로 해서 입체 선택적으로 반응을 조절함으로써 다양한 광학활성표적 화합물을 합성하는 방법이 활발히 진행되고 있다. chiral building block의 제조 방법을 살펴보기로하자.