• 제목/요약/키워드: 활성토

검색결과 416건 처리시간 0.24초

Mass-production of Eleutherococcus seoulensis Seedlings Through Somatic Embryogenesis (체세포배 형성을 통한 서울오갈피(Eleutherococcus seoulensis) 묘목의 대량생산)

  • Lee, Su-Gwang;Kang, Ho-Duck
    • Journal of Korean Society of Forest Science
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    • 제98권6호
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    • pp.719-725
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    • 2009
  • This study was conducted to establish the optimal condition for acclimatization from somatic embryos of Eleutherococcus seoulensis. Torpedo-shaped embryos of Eleutherococcus seoulensis were cultured on 1/3 MS and WPM media supplemented with $GA_3$ (3.0, 5.0 mg/L) for 4 weeks. Plentlets were transferred to 1/2 SH solid medium with 1.0 mg/L $GA_3$ and 0.2% activated charcoal for shoot and root elongation and them elongated plantlets further developed on 1/2 SH medium for 4 weeks. Developed plantlets further elongated into well-shaped leaf and root system on 1/3 SH medium under ventilation condition for 4 weeks. Plantlets grew normally on 1/3 SH basal medium, were acclimated on various soil. Survival frequency of plantlets was influenced by soil type(peatmoss+perlite, perlite, soil on Nam mountain). The highest survival rate to soil was more than 70% when plantlets were 1/3 SH medium under ventilation condition in Nam mountain soil. These results indicate that the systematic procedure of plant production in Eleutherococcus seoulensis could be practically applicable for mass propagation.

Information Modeling for Construction Equipment Interface in Earthwork (토공작업 건설장비 정보 인터페이스를 위한 정보 모델링)

  • Moon, Sungwoo;Cho, Kyeongsu
    • KSCE Journal of Civil and Environmental Engineering Research
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    • 제35권3호
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    • pp.699-706
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    • 2015
  • Earthwork is executed to provide a space for facility structures, and plays an important role in the construction operation. Contractors usually put a combination of construction vehicles to execute the earthwork operation. These construction vehicles need to interface with each other for productivity improvement. The objective of this study is to prototype an earthwork supportive system for information interface between construction vehicles. The study is conducted in a sequence of 1) analysis of information interface during earthwork; 2) data modeling of earthwork attributes; and 3) development of a prototype for the earthwork supportive system. The output screen images of the prototype show that the earthwork supportive system can improve communication between construction vehicles by facilitating information interface during earthwork.

Characterization of Fe (III)-Reducing Bacteria Isolated from the Sediment of Chunho Reservoir (천호지 저질토에서 분리한 철환원세균의 특성)

  • 안태영;박재홍;이일규;전은형
    • Korean Journal of Microbiology
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    • 제38권2호
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    • pp.133-138
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    • 2002
  • Microbial Fe (III) reduction is important for the biogeochemical cycle in the sediment of freshwater system. Also, the Fe (III) reducing mechanism make a model of oxidizing organic compounds and reducing toxic heavy metals, such as chrome or uranium. Thirty-seven strains which have Fe (III) reducing activity were isolated from sediments in lake Soyang and Chunho reservoir. The initial concentration of Fe (II) was the highest in sediments of lake Soyang. However, the highest Fe (III) reducing activity was shown in Chunho reservoir. All isolates were tested for Fe (III) reducing activity. Strains C2 and C3, which were isolated from sediments of Chunho reservoir, showed the highest activity. These strains were tested to see if they utilize various electron donors such as glucose, yeast extract, acetate, ethanol and toluene. Significantly, glucose and yeast extract were used as electron donors. Also these strains were conformed to use humid acid and nitrate as electron accepters. The 16S rRNA sequences of strains C2 and C3 were closely related to Aeromonas hydrophila with 95% similarity.

Evaluation of Biological Activity on Hawthorn Tree (Crataegus pinnatifida) Extracts (산사나무 추출물의 생리활성 평가)

  • Min, Hee-Jeong;Kim, Young-Kyoon;Bae, Young-Soo
    • Journal of the Korean Wood Science and Technology
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    • 제45권3호
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    • pp.317-326
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    • 2017
  • The wood and bark of Hawthorn tree (Crataegus pinnatifida Bunge) were immersed with 70% aqueous acetone for 3 days. After filtering, the wood extracts were fractionated with n-hexane, chloroform ($CHCl_3$), n-butanol (n-BuOH) and $H_2O$, and the bark extracts were fractionated with n-hexane, $CHCl_3$, ethylacetate (EtOAc) and $H_2O$. Then antioxidative and anti-inflammatory activities were evaluated on each fraction. Antioxidative activity indicated high activity in the n-butanol soluble fraction of wood and in the EtOAc soluble fraction of bark. Especially, the EtOAc soluble fraction of bark showed much higher antioxidative value compared to the controls, buthylated hydroxytoluene (BHT) and ${\alpha}$-tocopherol. In the anti-inflammatory activity, all of the tested fractions except the $H_2O$ soluble fraction of wood showed high inhibitory effect on nitric oxide (NO) production. Based on the above results, the extracts of hawthorn tree may be applied for one of the natural biomass sources that can be used as an antioxidant and an anti-inflammatory substance.

Evaluation of Biological Activity on The Hydrolyzable Tannins of Katsura Tree (Cercidiphyllum japonicum) (계수나무 가수분해형 탄닌의 생리활성 평가)

  • Min, Hee-Jeong;Lee, Min-Sung;Kim, Young-Kyoon;Bae, Young-Soo
    • Journal of the Korean Wood Science and Technology
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    • 제45권3호
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    • pp.250-257
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    • 2017
  • Eight hydrolyzable tannin compounds, such as gallic acid (1), methyl gallate (2), kurigalin (3), 1,2,3,6-tetra-O-galloyl-${\beta}$-D-(+)-glucose (4), 1,2,3,4,6-penta-O-galloyl-${\beta}$-D-(+)-glucose (5), 6-m-digalloyl-1,2,3,4,6-penta-O-galloyl-${\beta}$-D-(+)-glucose (6), isocorilagin (7), macabarterin (8), were isolated from the EtOAc and $H_2O$ soluble fractions of Katsura tree (Cercidiphyllum japonicum) leaves, wood and bark. Then antioxidative and anti-inflammatory activity were evaluated on the each isolated compound. The antioxidative test was DPPH radical scavenging activity and all of the isolated compounds indicated much higher antioxidative values compare to the controls, BHT and ${\alpha}$-tocopherol. In the anti-inflammatory test measuring nitric oxide (NO) inhibition activity, methyl gallate, 1,2,3,6-tetra-O-galloyl-${\beta}$-D-glucose and 1,2,3,4,6-penta-O-galloyl-${\beta}$-D-glucose inhibited NO production, and especially, methyl gallate showed high inhibition activity. However, the anti-inflammatory activity of the hydrolyzable tannins did not show positive effect. Based on the above results, the hydrolyzable tannins of katsura tree may be used as one of the natural biomass sources that can substitute with the synthetic antioxidant.

Isolated Activation of the Upper Trapezius in Three Manual Muscle Testing Position : Convergence Study (세 가지 도수근력 검사 자세에서 상부 승모근의 독립적 수축 : 융합적 연구)

  • Ha, Sungmin
    • Journal of the Korea Convergence Society
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    • 제11권1호
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    • pp.83-88
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    • 2020
  • The purpose of this study was to investigate the optimal position among three manual muscle tested positions for upper trapezius in which to obtain an isolated upper trapezius EMG signal for the normalization of upper trapezius muscle EMG activity. A total of 28 healthy adult men participated in the experiment. The UT (upper trapezius) and LS (levator scapulae) muscle activities were measured using the TeleMyo 2400T and analyzed using MyoResearch software. The muscle activity of the US and LS was measured by performing three manual muscle test positions for the upper trapezius. The UT/LS ratio during the S-MVIC was 80.25 and was significantly higher than that during the T-MVIC (76.50; p = 0.011) and the C-MVIC (60.95; p < 0.001). And, the UT/LS ratio during the T-MVIC and was significantly higher than that during the C-MVIC (p < 0.001). Based on the results of present study, we suggest a switch from T-MVIC to S-MVIC for the independent normalization reliability of upper trapezius EMG activity. The UT muscle strength or normalization test using S-MVIC will be able to measure muscle strength or activity of UT compared to previous measurement methods.

Change of isometric contractile force and muscle activity applying ice and hot according to the time on biceps brachii muscle (온열 및 한랭의 적용시간에 따른 상완이두근의 등척성 수축력과 근활성도 변화)

  • Lee, Joon-Hee;Jeon, Jae-Keun
    • Journal of Digital Convergence
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    • 제10권11호
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    • pp.459-465
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    • 2012
  • This study has investigated the effect of isometric contractile force and muscle activity applying ice and hot according to the time from the biceps brachii muscle. In this study, 20 university students participants without musculoskeletal and neurological disorders. By applying a hot pack 5 min, 10 min, 20 min and 30 min respectively and ice pack for 5 min, 10 min, and 20 min respectively. After that measurement are skin temperature, contractile force and muscle activity. Skin temperature of the hot and cold 5 min applied that rapidly changing. Increasing the time it takes to apply a variance has been reduced(p<.001). Isometric contractile force was not statistically significant but highest when applying the hot pack 5 minutes and lowest when applying the hot pack 30 minutes(p<.001). Muscle activity and median frequency was highest when applying the hot pack 5 minutes. To analyze the above results, it was found that isometric contractile force and muscle activity changed according to the applying time. These result lead us to the conclusion that this study will be more evidence for changes in muscle contraction to apply hot pack and ice pack on clinic.

Antioxidative and Free Radical Scavenging Effects of Rhus javanica Linne (오배자의 항산화 및 자유라디칼 소거효과)

  • Cha, Bae-Cheon;Lee, Sung-Bae
    • Korean Journal of Medicinal Crop Science
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    • 제6권3호
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    • pp.181-187
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    • 1998
  • This study was carried out to investigate the antioxidative activities of medicinal plants. Through the examination of methanol extracts from 30 species for radical scavenging effects using DPPH method, the extracts from Rhus javanica Linne, Smilax china Linne and Polygonum cuspidatum Siebold et Zucarinii showed strong antioxidative activity. Because of its highest antioxidative activity among 30 medicinal plants, radical scavenging effects of 4 different extract compartments (n- Hexane, EtOAc, BuOH and $H_2O$ extracts) from Galla Rhois MeOH extract of Rhus javanica Linne were examined by DPPH method and antioxidant effects on the 4 different extract compartments were tested by Ferric-Thiocyanate method. Antioxidative activities of n- Hexane, EtOAc and BuOH extracts were similar or even higher than that of natural (tocopherol) or synthetic antioxidants (BHA), suggesting that major fractions for the antioxidative activity of Rhus javanica Linne were the n- Hexane, EtOAc and BuOH extract compartments.

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Physiological Characteristics and Tyrosinase Inhibitory Activity of Lactobacillus plantarum M23 Isolated from Raw Milk (원유에서 분리한 Lactobacillus plantarum M23의 Tyrosinase 활성 저해 및 생리적 특성조사)

  • Heo, In-Sook;Kim, Kee-Sung;Yang, Seung-Yong;Lee, Nam-Hyouck;Lim, Sang-Dong
    • Food Science of Animal Resources
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    • 제27권4호
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    • pp.501-508
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    • 2007
  • In order to develop a new starter for fermented milk, Lactobacillus plantarum M23 was isolated from raw milk and investigated for physiological characteristics. It showed good tyrosinase inhibitory activity compared with commercial lactic acid bacteria. The optimum growth temperature of Lactobacillus plantarum M23 was $40^{\circ}C$ and cultures took 17 hr to reach pH 4.3. Lactobacillus plantarum M23 showed more sensitivity to Penicillin-G, Oxacillin, Novobiocin, Chloramphenicol in a comparison of 12 different antibiotics, and showed most resistance to Vancomycin. It showed higher leucine arylamidase and ${\beta}-galactosidase$ activities compared to 16 other enzymes. It was comparatively tolerant to bile juice and able to survive at pH 2 for 3 hours. It showed high resistance to Escherichia coli, Salmonella typhimurium and Staphylococcus aureus with rates of 77.8%, 86.5% and 83.8%, respectively. Based on these and previous results, Lactobacillus plantarum M23 could be an excellent starter culture for fermented milk with high resistance to melanin.

Comparison of Melanogenesis-Inhibiting Activity by Extracts of Prunus persica Flower and Calyx (도화 및 도화악 추출물의 미백활성 비교)

  • Son, Hyeong-U;Lee, Suk Hee;Kim, Min-A;Park, Hee-Joon;Lee, Sang-Han
    • Food Science and Preservation
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    • 제19권6호
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    • pp.946-950
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    • 2012
  • The antioxidant activity and whitening effect of the distilled water (DW) and ethanol extracts of the Prunus persica flower and calyx were studied. In the oxygen radical absorption capacity (ORAC) assay for antioxidant activity measurement, it was confirmed that the flower extract was stronger than the calyx extract, and that the ethanol extract was relatively stronger than the DW extract. To define the whitening effect, an experiment was conducted involving tyrosinase inhibitory assay and measurement of the melanin content of B16F10. As a result of the use of tyrosinase, the DW extract of calyx showed 53% inhibition as the highest activity. The melanin content inhibitory rates were defined as 57% for the ethanol flower extract and 63% of the ethanol calyx extract, based on a $10{\mu}g/mL$ concentration. Based on these results, mixture with the whitening effect in the extract of P. persica and another compounds should be researched for development as a cosmetic ingredient.