• Title/Summary/Keyword: 활성세균수

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Structure-activity Analysis of Benzylideneacetone for Effective Control of Plant Pests (벤질리덴아세톤 화학구조 변이에 따른 생리활성 변화 분석 및 식물 병해충 방제 효과)

  • Seo, Sam-Yeol;Jun, Mi-Hyun;Chun, Won-Su;Lee, Sung-Hong;Seo, Ji-Ae;Yi, Young-Keun;Hong, Yong-Pyo;Kim, Yong-Gyun
    • Korean journal of applied entomology
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    • v.50 no.2
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    • pp.107-113
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    • 2011
  • Benzylideneacetone (BZA) is a compound derived from culture broth of an entomopathogenic bacterium, Xenorhabdus nematophila (Xn). Its immunosuppressive activity is caused by its inhibitory activity against eicosanoid biosynthesis. This BZA is being developed as an additive to enhance control efficacy of other commercial microbial insecticides. This study was focused on the enhancement of the immunosuppressive activity of BZA by generating its chemical derivatives toward decrease of its hydrophobicity. Two hydroxylated BZA and one sugar-conjugated BZA were chemically synthesized. All derivatives had the inhibitory activities of BZA against phospholipase $A_2$ ($PLA_2$) and phenoloxidase (PO) of the diamondback moth, Plutella xylostella, but BZA was the most potent. Mixtures of any BZA derivative with Bacillus thuringiensis (Bt) significantly increased pathogenicity of Bt. BZA also inhibited colony growth of four plant pathogenic fungi. However, BZA derivatives (especially the sugar-conjugated BZA) lost the antifungal activity. These results indicated that BZA and its derivatives inhibited catalytic activities of two immune-associated enzymes ($PLA_2$ and PO) of P. xylostella and enhanced Bt pathogenicity. We suggest its use to control plant pathogenic fungi.

Antimicrobial activity of epigallocatechin gallate from green tea (Camellia sinensis) on pathogenic Salmonella Enteritidis in braised quail eggs (메추리알 장조림에서 녹차 Epigallocatechin gallate (EGCG)의 Salmonella Enteritidis에 대한 저장 온도에 따른 항미생물 활성)

  • Kim, Kwang-Yeop;Kim, Young-Ji;Kim, Hong-Seok;Song, Kwang-Young;Kim, Dong-Hyeon;Lee, Mi-Young;Kim, Eui-Su;Jeong, Heon-Sang;Seo, Kun-Ho
    • Korean Journal of Food Science and Technology
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    • v.48 no.4
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    • pp.329-334
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    • 2016
  • The inhibitory effect of epigallocatechin gallate (EGCG), one of the antioxidants in green tea (Camellia sinensis), against Salmonella Enteritidis was evaluated in commercial braised quail eggs at two temperatures (4 and $25^{\circ}C$). Although S. Enteritidis was dose-dependently suppressed by EGCG in pure culture at $25^{\circ}C$, it was not inhibited in the sauce or eggs at this temperature. At low temperature ($4^{\circ}C$), S. Enteritidis was inhibited in both the sauce and eggs by $400{\mu}g/mL$ EGCG. Thus, EGCG at an appropriate concentration could be a useful food additive for inhibiting S. Enteritidis in braised quail eggs at low temperatures.

Quality Characteristics and Storage Improvement of Seasoned Pork Added with Phlomis umbrosa Turcz. Extracts (속단추출물의 첨가로 인한 양념 돈육의 품질특성 및 저장성 향상)

  • Kim, Hyun-Jeong;Kim, Mi-Jin;Oh, Song-I;Jung, Young-Tae;Park, Soo-Kyoung;Lee, In-Seon
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.40 no.1
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    • pp.102-109
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    • 2011
  • Antioxidant and antibacterial activities of Phlomis umbrosa Turcz. (PU) ethanol extracts were investigated in vitro. Total polyphenols, flavonoids and FRAP values of PU extracts were $57.32{\pm}2.30{\mu}g$/mg, $21.44{\pm}2.52{\mu}g$/mg and 0.26 mmol $Fe^{2+}$/g, respectively. The PU extracts also showed strong DPPH scavenging activity and antibacterial effects against Staphylococcus aureus. In addition, the DPPH scavenging effect of the seasoning sauces was increased by the addition of PU extracts. This study was also conducted to evaluate the quality characteristics of seasoned pork treated with PU extracts during storage at $4^{\circ}C$ for 21 days. Seasoned pork was produced containing 0, 0.5, and 1% PU extracts and 0.1% BHA. The total bacteria and TBARS values of seasoned pork added with PU were decreased compared to the control, whereas coliform bacteria were not detected after longer storage periods. With regards to sensory evaluation, seasoned pork treated with 0.5% PU had higher juiciness, flavor and overall acceptability compared to seasoned fork. These results suggest the possibility of manufacturing seasoned pork with PU extracts for the improvement of shelf-life and quality.

Quality Evaluation of Dried Red Pepper After Copper Plate Contact (구리표면 접촉에 의한 건조 고춧가루의 특성 평가)

  • Oh, Yeong Ji;Park, Sun Young;Song, Eiseul;Kang, Smee;Hong, Jungil
    • Journal of Food Hygiene and Safety
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    • v.35 no.2
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    • pp.177-182
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    • 2020
  • Copper possesses antimicrobial properties against certain bacteria and fungi. In this study, changes in microbial population, color and the antioxidant properties of dried red pepper powder (RPP) by contact with the surface of a copper plate were investigated. Lightness of RPP increased after treatment on the copper plate for 2 h at 55℃. The numbers of total aerobic mesophilic bacteria in RPP increased by 0.61 and 0.50 log on the surface of stainless steel and copper plates during 24 h at 37℃, respectively. The population of yeast and mold was reduced by 90% in the RPP treated on both copper and stainless steel plates under the same condition. Yeast and mold were significantly reduced on the copper plate during 2 h at 55℃. Scavenging activities of RPP treated on the copper plate for 24 h at 37℃ against DPPH radical were decreased; those for 2 h at 55℃ against ABTS radical were rather enhanced. The results suggest that copper might be applied in the processing and storage equipment in relation to RPP products for antimicrobial purposes.

Comparative Study of the Biological Activity of Propolis Extracts with Various Countries of Origin as Cosmetic Materials (원산지별 프로폴리스 추출물의 화장품 소재로서의 생리활성 비교연구)

  • Jung, Eunsun;Weon, Jin Bae;Ji, Hyanggi;You, Jiyoung;Oh, Se-young;Kim, Hayeon;Xin, Yingji;Kim, Eun Bin;Heo, Kang-Hyuck;Park, Deokhoon
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.46 no.2
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    • pp.159-166
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    • 2020
  • Propolis is a sticky resinous substance that is formed by the combination of honeybee secretions and resin of plants, which serves to protect from bacteria and viruses. This study aims to evaluate the efficacy of propolis extract from Korea (KPE), China (CPE), and Brazil (BPE) through antioxidant, antibacterial, whitening, and anti-inflammatory tests, and to examine their potential as cosmetic materials. KPE, CPE, and BPE showed significant antioxidant activities on flavonoid/polyphenol content and free radical scavenging activity. The antibacterial effect of propolis on skin flora was determined by measuring the minimal inhibitory concentration (MIC). KPE showed better antibacterial efficacy than CPE and BPE in C. acnes (KPE, CPE, and BPE: (62.5, 250, and 500) ㎍/mL, respectively). Furthermore, KPE inhibited the melanin synthesis in human epidermal melanocytes and production of nitric oxide and PGE2 induced by lipopolysaccharide (LPS) in mouse macrophages, which showed better than did CPE or BPE. Taken together, the propolis extracts can be applied to antioxidant, antibacterial, and anti-inflammatory ingredient for cosmetics, while KPE showed superior potential in antibacterial, anti-inflammatory, and whitening efficacies.

Effect of Storage Conditions on the Microbiological and Physicochemical Characteristics of Traditional Kochujang (저장조건에 따른 전통고추장의 미생물 및 이화학적 특성 변화)

  • Kim, Dong-Han;Kwon, Young-Mi
    • Korean Journal of Food Science and Technology
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    • v.33 no.5
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    • pp.589-595
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    • 2001
  • Effect of anti-microbial agents, such as alcohol, garlic, chitosan, K-sorbate, or mustard, or pasteurization on the quality of traditional kochujang was investigated during storage at $30^{\circ}C$ for 24 weeks. Water activities of kochujang decreased after 12 weeks of storage. Consistency increased during storage and highest consistency of kochujang was obtained by the addition of mustard or garlic. Hunter a- and b-values decreased linearly as storage time passed. The degree of increase in total color difference$({\Delta}E)$ of alcohol added group was the highest among the tested anti-microbial agents. Gas was rapidly produced in the control and chitosan added group of kochujang. Capsanthin content decreased more rapidly in the group of alcohol, garlic or mustard added kochujang than the other group. Viable cell counts of yeast and aerobic bacteria in kochujang increased up to 12 weeks of storage, and then decreased slowly. The number of yeast was low in K-sorbate or alcohol added group. Activity of ${\alpha}-amylase$ decreased during storage, but that of ${\beta}-amylase$ increased in alcohol, garlic, K-sorbate added or pasteurized kochujang. Protease activities did not show any remarkable differences in the groups of tested during storage.

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A Study on the Antimicrobial Activity of Snowberry Extract (스노우베리 추출물의 항균 활성에 관한 연구)

  • Chanwoo Lee;Hye-Yeon Heo;Yu-Jin Park;YoungPyo Jang;Bo Ae Kim
    • Journal of the Korean Applied Science and Technology
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    • v.41 no.2
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    • pp.283-291
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    • 2024
  • This study compared and evaluated the antibacterial activities and MIC of snowberry extract and fermented extract. For antibacterial activity, Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas aeruginosa, Escherichia coli, and Candida Albicans were used. Antimicrobial activity and MIC were measured at concentrations of 10, 50, 100, and 200 mg/mL. Antibacterial activity was measured using the 8mm paper disc method. In the case of snowberry extract, it was confirmed that the root extract showed antimicrobial activity at concentrations of 100 mg/mL and 200 mg/mL, and in the case of fermented extract, it showed antimicrobial activity at 200 mg/mL. As a result of the MIC, for fermentation in snowberry leaf and root extracts, the minimum inhibitory concentration for each bacterium was confirmed in stem extracts. The above results indicate that the antibacterial properties of snow berries are improved by fermentation.

Adhesion control of Campylobacter jejuni in chicken skin using emulsifiers (유화제를 이용한 계육 표면에서 Campylobacter jejuni의 부착 제어)

  • Oh, Do Geon;Kim, Kwang Yup
    • Korean Journal of Food Science and Technology
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    • v.52 no.6
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    • pp.670-677
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    • 2020
  • To prevent contamination by Campylobacter jejuni during chicken carcass processing, the effect of emulsifiers on C. jejuni inoculated on chicken skin was investigated using confocal laser scanning microscopy. Among the 8 emulsifiers (SWA-10D, L-7D, M-7D, S-1670, L-1695, P-1670, polysorbate 20, polysorbate 80) tested for antimicrobial activity by the paper disk method, 4 emulsifiers (L-7D, L-1695, polysorbate 20, polysorbate 80) were screened further. Emulsifier L-1695 showed the largest clear zone at a concentration of 200 mg/mL. The 4 emulsifiers subjected to primary screening were screened for heat and pH stability. In the contact surface test, emulsifier L-1695 showed the lowest log CFU/㎠ value on both stainless steel and ceramic surfaces. When emulsifier L-1695 was applied via general and electrostatic spray methods, the number of C. jejuni entrapped inside chicken skin follicles was significantly reduced in both methods. In conclusion, the emulsifier L-1695 could be employed as a microbial detachment agent in the chicken carcass processing industry.

Properties on the Quality Characteristics and Microbial Change during Storage added with extracts from Ulmus cortex (느릅나무 추출액을 첨가한 식빵의 품질 특성 및 저장기간에 따른 미생물의 변화)

  • Jeon, Jeong-Ryae;Kim, Jean
    • Korean journal of food and cookery science
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    • v.20 no.2
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    • pp.180-186
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    • 2004
  • Evidence shows there are flavonoid and polyphenol compounds in Ulmus cortex which has excellent antioxidant, anticancer and antimicrobial properties. This study summarizes our findings on effects of Ulmus cortex extracts(UDE) on quality characteristics of bread and the changes of microbial counts during storage. Dough yield and specific loaf volume of bread decreased significantly because the amount of UDE increased. Lightness of bread surface, redness of crumb and yellowness of crust were significantly increased. Texture evaluation showed that hardness was the highest in the bread prepared with 30% UDE. Sensory evaluation showed that odor, taste, texture, and overall acceptability were the best in bread which was made with 10% UDE. At the beginning of the period of storage, there were not significant differences of microbial cell count as increasing UDE, but significant decreasing was observed after passing 6 days. A negative correlation was generally observed between the sensory and mechanical properties. The results of total microbial count show that adding UDE in processing bread extend bread's storage time.

Correlation Between food Processing-Associated Stress Tolerance and Antimicrobial Resistance in Food Pathogens

  • Woode, Benjamin Kojo;Daliri, Frank;Daliri, Eric Banan-Mwine
    • Journal of Food Hygiene and Safety
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    • v.35 no.2
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    • pp.103-108
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    • 2020
  • Recently, consumer demand for safe but minimally processed food has rapidly increased. For this reason, many food processing industries are applying hurdle technology to enhance food safety, extend shelf life, and make foods appear minimally processed. Meanwhile, studies have shown that a treatment (stress) meant to inactivate foodborne pathogens may trigger adaptation mechanisms and could even offer cross protection against subsequent treatments. Also, certain routine farm practices such as antibiotic and herbicide use could result in the development of antibiotic-resistant pathogens. Such bacteria may be tolerant to food processing-associated stress and be more likely to remain viable in processed foods. In this review, we discuss the correlation between food processing-associated stress and antibiotic resistance. We also discuss molecular mechanisms such as the use of sigma factors, SOS response pathways and efflux pumps as means of cross protection against antimicrobial compounds and other food processing-associated stresses.