• Korean Journal of Environmental Biology
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• v.27 no.2
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• pp.230-236
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• 2009

An extract of Allomyrina dichotoma larva (ADL), one of the insects used most frequently in traditional Chinese medicine for the treatment of liver diseases such as hepatocirrhosis and hepatofibrosis, was assessed for antioxidant bioactivity in this study. In the current work, we have investigated the protective effects of ADL extracts on tert-butyl hydroperoxide (t-BHP)-induced hepatotoxicity in cultured hepa1c1c7 cells and in the mouse liver. The treatment of the hepa1c1c7 cells with ADL extracts induced a significant reduction of t-BHP-induced oxidative injuries, as determined by cell cytotoxicity, lipid peroxidation (LPO) and reactive oxygen species contents, in a dose-dependent manner. Moreover, ADL extracts evidenced a protective effect against t-BHPinduced oxidative DNA damage, as revealed by the results of the Comet assay in hepa1c1c7 cells. ADL extracts also protected against hydroxyl radical-induced 2-deoxy-d-ribose degradation by ferric ion-nitrilotriacetic acid and $H_2O_2$. In addition, ADL extracts were shown to be able to quench 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radicals. Our in vivo study revealed that ADL extracts pretreatment applied prior to t-BHP administration significantly prevented an increase in the serum levels of hepatic enzyme markers and reduced LPO in the mouse liver in a dose-dependent manner. Taken together, these results suggest that the protective effects of ADL extracts against t-BHP-induced hepatotoxicity may be attributable, at least in part, to its ability to scavenge free oxygen radicals, and to protect against DNA damage due to oxidative stress.

• The Korean Journal of Mycology
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• v.46 no.1
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• pp.1-8
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• 2018

Three fungal isolates, NNIBRFG108, 1139, and 1480, were isolated from freshwater environments; NNIBRFG108 from plant litter in Samcheok, Gangwon and NNIBRFG1139 and 1480 from the soil in Jeju & Gimcheon, Gyeongbuk, Korea. Based on the morphological characteristics and phylogenetic analysis of internal tanscribed spacer (ITS), 28S rDNA region, and ${\beta}$-tubulin gene, NNIBRFG108, NNIBRFG1139, and NNIBRFG1480 isolates were confirmed as Didymella segeticola, D. ellipsoidea, and D. aeria, respectively. Neither species has previously been described in Korea.

• Korean Journal of Plant Resources
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• v.16 no.3
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• pp.257-263
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• 2003

The NDK1 is an ubiquitous enzyme that transfer phosphate groups from triphosphate nucleoside diphosphates(NDPs) in eukaryotes and prokaryotes. We isolated and characterized a cDNA encoding a nucleoside diphosphate kinase 1(CNDK 1) in Codonopsis lanceolata. The CNDK 1 is 444bp long and open reading frame of 447bp with a deduced amino acid of 148 residue. The CNDK 1 has an ATP binding site in 12­16 residue and phosphohistidine intermediate in 115 residue of amino acid sequence. Although several NDK 1 genes have been cloned in plants, but little is known about the functional significance of this enzyme during plant growth and development. The CNDK 1 shows the identities to Arabidopsis thaliana (71％), Oryza sativa(75％), Glycine max (79％), Brassica rapa (77％), Mesembryanthemum crystallinum (85 ％), Spinacia oleracea (83％), Pisum sativum (82％). The CNDK 1 of C. laceolata have a closer relationship of Glycine max and Pisum sativum at the phylogenic analysis.

• Research in Plant Disease
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• v.25 no.1
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• pp.33-37
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• 2019

From 2016 to 2018, approximately 15% of kohlrabi were observed displaying significant clubroot symptoms in farmer's fields in Jeju, Korea. The initial infection appeared as hypertrophy of root hairs, and as the disease progressed, galls formation occurred on the main roots, finally disease progress resulted in yellowing and wilting of leaves. Pathogenicity was proven by artificial inoculation of plants with resting spore suspension, fulfilling Koch's postulates. The resting spore is one-celled, spherical and subspherical, colorless, and $3-5{\mu}m$ in diameter. On the basis of the morphological characteristics and phylogenetic analyses of internal transcribed spacer rDNA, the causal agent was identified as Plasmodiophora brassicae. To our knowledge, this is the first report on the occurrence of P. brassicae on kohlrabi in Korea.

• Environmental Engineering Research
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• v.18 no.3
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• pp.139-143
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• 2013

This study was performed to examine the in vitro toxicities which are incurred due to the mobilization metals from standard reference material (SRM) 1648, fine ($PM_{2.5}$), and coarse ($PM_{10}$) particulate matter collected in Seoul metropolitan area. DNA single strand breaks of approximately 74% and 62% for $PM_{2.5}$ and for $PM_{10}$, respectively, were observed in the presence of chelator (EDTA or citrate)/reductant (ascorbate), as compared to the control by 2% without chelator or reductant. $PM_{2.5}$ induced about 40% more carbonyl formation with proteins in the presence of EDTA/ascorbate than $PM_{10}$. Therefore, more damage to biomolecules was incurred upon exposure to $PM_{2.5}$ than to $PM_{10}$. The treatment of a specific chelator, desferrioxamine, to the reaction mixture containing chelator plus reductant decreased the extent of damage to DNA to the level of the control, but did not substantially decrease the extent of damage to proteins. This suggests that different arrays of metals were involved in the oxidation of DNA and proteins.

• Environmental Analysis Health and Toxicology
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• v.24 no.2
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• pp.107-117
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• 2009

Metal pollution of aquatic ecosystems is a problem of economic and health importance. Information regarding molecular responses to metal exposure is sorely needed in order to identify potential biomarkers. To determine the effects of heavy metals on chironomids, the full-length cDNA of alcohol dehydrogenase (ADH3) from Chironomus riparius was determined through molecular cloning and rapid amplification of cDNA ends (RACE). The expression of ADH3 was analyzed under various cadmium and copper concentrations. A comparative and phylogenetic study among different orders of insects and vertebrates was carried out through analysis of sequence databases. The complete cDNA sequence of the ADH3 gene was 1134 bp in length. The sequence of C. riparius ADH3 shows a low degree of amino acid identity (around 70%) with homologous sequences in other insects. After exposure of C. riparius to various concentrations of copper, ADH3 gene expression significantly decreased within 1 hour. The ADH3 gene expression was also suppressed in C. riparius after cadmium exposure for 24 hour. However, the effect of cadmium on ADH3 gene expression was transient in C. riparius. The results show that the suppression of ADH3 gene by copper exposure could be used as a possible biomarker in aquatic environmental monitoring and imply differential toxicity to copper and cadmium in C. riparius larvae.

• Korean Journal of Ecology and Environment
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• v.36 no.3 s.104
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• pp.221-234
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• 2003

Phylogenetic studies would clarify the diversity of fishes if the morphological analysis based on plesimorphy characters combined with new genetic analysis on molecular level, inferring more accurate and objective phylogeny and the taxonomy. Current molecular phylogenetic approach using mitochondrial genome provides the framework for a new hypothesis not only inferring the relationships between ancestor descendants but raveling the intra-, interspecies variation.

• Electronics and Telecommunications Trends
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• v.36 no.1
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• pp.109-119
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• 2021

The Korean government recently announced a plan of the Carbon Neutral policy in addition to the Green New Deal of the Korean New Deal and the Renewable Energy 3020. The energy sector is entering the era of major transformation involving the expansion of decarbonization, decentralization, and digitalization. DNA-oriented ICT technology will be incorporated into the sector. Further, new energy industries and services are being realized via efficient and smart operation and by appropriately managing the energy-environment changes. Recently, ETRI presented a technology development map for 2035 comprising 12 new concepts in four major fields(personal, social, industrial and public) of national intelligence. This map includes the concept of "Energy Maestro" associated with the field of public intelligence for human sustainability. This paper briefly introduces this concept and ETRI's Energy-R&D status. Based on the domain knowledge and the experience acquired through the R&D, ETRI will lead to a new paradigm with respect to the creation of new energy services and industries via the incorporation of the new ICT technologies including AI and big-data into the energy sector.

• Korean Journal of Ichthyology
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• v.34 no.1
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• pp.1-7
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• 2022

A larva of the deep-sea angler fish, Himantolophus groenlandicus (2.2 mm BL), identified based on the complete mitochondrial DNA sequence, was collected at the surface of the western North Pacific. The postflexion stage larva had a round body, small teeth, incipient dorsal fin rays, eyes slightly recessed in the lower part, and melanophores on the gills and parietal and dorsal regions. These morphological features differ from a description of a larva reported as the same species with similar size (2.1 mm BL). The genetic and morphological information of our specimen should be useful for identifying larval H. groenlandicus.

• 한국문화재보존과학회:학술대회논문집
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• 2005.11a
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• pp.252-254
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• 2005

박물관 수장고와 전시실 내의 유기물 문화재는 실내공기에 부유하고 있는 먼지와 곰팡이로 인해 생물적 피해를 받는다. 미생물로 인한 피해를 줄이기 위하여 박물관에서는 환경 조절법을 시행하거나 수장고와 전시실 내에 보존약제를 놓거나 또는 훈증 처리를 통한 방균 방제처리를 시행하고 있다. 미생물의 발생은 문화재 표면의 착색, 재질 분해, 변질 등을 야기한다. 본 연구는 위에 제시된 방법 외에 공기청정기를 이용하여 실내 부유 미생물 수를 줄여 유기물 문화재의 피해를 줄일 수 있는지를 알아보았다. 국립문화재연구소 내 생물과학실의 실험실 한 곳을 임의로 지정하여 공기청정기를 설치하기 전 곰팡이의 수를 측정하고, 공기청정기를 설치한 후의 곰팡이 변화를 4일 간격으로 측정하였다. 또한, 공기 중의 ???r성분 분석을 위하여 가스크로마토그래피(Gas Chromatography)를 이용하였다. 측정결과 곰팡이의 수는 대체적으로 감소하였으며, 채취한 곰팡이를 배양하여 동정을 실시하였다. 곰팡이 동정은 현미경을 통한 형태학적 분류와 ITS(Internal Transcribed Sequence)를 이용한 rDNA(Ribosomal DNA) PCR(Polymerase Chain Reaction)분석 두 가지 방법을 이용하여 동정하였다.