• Proceedings of the Korea Environmental Mutagen Society Conference
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• 2004.05a
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• pp.63-63
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• 2004

• Proceedings of the Korea Environmental Mutagen Society Conference
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• 2004.05a
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• pp.73-73
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• 2004

• Proceedings of the Korea Society of Environmental Toocicology Conference
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• 2004.11a
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• pp.30-31
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• 2004

• Journal of the Korean Society of Marine Environment & Safety
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• v.24 no.2
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• pp.171-178
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• 2018

The ocean is a harsh environment and Korea Coast Guard officers often face the threat of death or going missing in the line of duty. In order to promptly identify unknown bodies during mishaps, Korea Coast Guard Research Center has established an identification system using the DNA of its officers. This collected DNA can also be entombed in National Cemeteries when the remains are not recovered. The storage, disposal and quality control of the materials are overseen by the Governing Committee according to the Enforcement Rules for the Human Biological Materials Storage Project for the Identification of Officers Killed or Gone Missing in Action. Approximately 700 bodies are found per year along the Korean coast. An alternative method should be put in place for when identification through fingerprints and teeth are not applicable due to severe decomposition or partial recovery. We believe it would greatly contribute to helping identify the unknown bodies if the storage project could expand its data to include marine industry workers, relevant researchers and those involved in marine leisure activities.

• Korean Journal of Environmental Agriculture
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• v.37 no.2
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• pp.135-140
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• 2018

BACKGROUND: The Asian hornet (Vespa velutina nigrithorax), a wasp species, has attacked honey bee populations and affected the beekeeping industry in Korea over the past 15 years. However, little research has been done with this invasive species. In this study, we investigated the intestine bacterial microbiota of Asian hornets and honey bees to design an attractive trap for Asian hornets. METHODS AND RESULTS: Genomic DNAs isolated from the intestine microorganisms of Asian hornets and honey bees were utilized to amplify bacterial 16S rDNA for the comparative sequence analysis. The next generation sequencing analysis identified that the orders Flavobacteriales as the most abundant intestinal microorganisms in Asian hornets, showing a clear difference compared to honey bees in which Aeromonadales are dominant. We also report five newly identified 16S rDNA sequences of Asian hornet intestinal bacteria. According to the sequence blast search, these five bacteria belong to the genera Thalassomonas, Caedobacter, Vampirovibrio, Alkaliphilus and Calothrix. CONCLUSION: While Asian hornets and honey bees show similar intestine bacterial diversity, the relative ratio of bacterial populations is different. providing useful information to design pest control agents specifically targeting Asian hornets.

• Journal of Yeungnam Medical Science
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• v.9 no.2
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• pp.248-255
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• 1992

Eight bacterial strains were examined whether they have phoP/phoQ genes which were known to be involved in the intracellular survival of Salmonella typhimurium. The phoP/phoQ operon were known to sense the stimuli of the genes involved in the adaptation of the environment. Using 514-basepairs EcoRV DNA fragment of phoP region of Salmonella typhimurium as a probe, dot blot hybridization were performed. Chromosomal DNAs of Klebsiella pneumoniae, Pseudomonas aeruginosa, Serratia marscescens, Enterobacter cloacae, Salmonella typhimurium, Escherichia coli, Shigella dysenteriae, and Listeria monocytogenes were examined by DNA hybridization assay. Against our expectation, intracellular pathogen, L. monocytogenes, did not have similar DNA sequences to phoP/phoQ of S. typhimurium, while E. coli, S. dysenteriae, and E. cloacae showed the positive signal even though they were not intracellular pathogens. This result suggested that the phoP/PhoQ operon was absent in intracellular pathogenic bacterias other than S. typhimurium. Rather it was found in phylogenetically closer bacterias to S. typhimurium, which were not able to survive in intracellular environment. Some different mechanism, which is not dependent on phoP/PhoQ operon, could be involved in the intracelluar survival of L. monocytogenes.

• Ocean and Polar Research
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• v.34 no.2
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• pp.137-149
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• 2012

Hemocyte parameters of the wild Pacific oyster Crassostrea gigas inhabiting intertidal zones in small bays (Gwangyang and Jinhae Bay) on the southern coast of Korea were evaluated using flow cytometry and neutral red retention (NRR) assay. Morphological features, cell count, mortality, DNA damage, phagocytosis, and lysosomal membrane stability of hemocytes were analyzed. Three types of hemocytes were identified in the oyster hemolymph: granulocytes, hyalinocytes, and blast-like cells. Immune related functions of hemocyte including phagocytosis and lysosomal membrane stability were significantly different among the study areas (P<0.05), while cell count, mortality, and DNA damage of hemocytes were not significantly different. In Gwangyang Bay, phagocytosis of granulocytes and lysosomal membrane stability of oyster hemocytes inhabiting inside bay were significantly lower than those of oyster hemocytes in outside bay (P<0.05), indicating that oysters in inside bay of Gwangyang were relatively suppressed the immunological function in hemocytes. Contrary to Gwangyang Bay, immune parameters of oyster hemocytes in Jinhae Bay not showed the difference between sampling sites. In conclusion, flow cytometry and NRR assay using oyster hemocyte has a powerful tool to investigate the cell level in a short time due to no-preprocessing of material.

• Korean Journal of Environmental Biology
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• v.38 no.1
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• pp.21-29
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• 2020

Morphology of a strain of unspecified Skeletonema species established from Korean coast was examined by light, fluorescence and scanning electron microscopy, and SSU(small subunit) and LSU(large subunit) rDNA of the strain were also sequenced. The specimen was characterized by solitary or short chains, and each cell contained 1-2 chloroplasts. The valve face was slightly convex, and the terminal fultoportula processes (TFPPs) were open and showed narrow distal ends that could be truncated or spiny with claw-like protrusions. The basal part of the TFPPs was tubular and oblique to the cell axis. The intercalary fultoportula processes (IFPPs) were also narrow, completely open, and joined in a 1 : 1 junction. Occasionally, one IFPP was connected with two opposing IFPPs. The morphological features of the specimen were identical to those of Skeletonema pseudocostatum. Molecular phylogeny based on SSU rDNA revealed that the Korean strain is nested within a clade comprising S. pseudocostatum and S. tropicum. However, based on D1-D2 LSU rDNA sequences, a clade including S. pseudocostatum and a Korean strain was separated from the S. tropicum clade. This indicates that the Korean strain can be identified as S. pseudocostatum. This species represents the first record from Korean coastal waters.

• Journal of Environmental Impact Assessment
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• v.29 no.3
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• pp.230-238
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• 2020

This study inferred the ecology of habitat use of the wild boars (Sus scrofa) in Yeongwol Hanbando wetland through DNA analysis using non-invasive samples of hairs. From November 2018 to May 2019, hair samples were collected from rubbing trees and hairtraps within the Hanbando wetland (2.772 ㎢). We extracted DNA from the hair samples and conducted PCR to verify the species and identify sex of the individuals. In addition we analysed 6 microsatellite markers to identify individuals and genetic relationship among the pairs of individuals. A total of 16 boar hairs were sampled, which turned out to be from 10 individual (7 females and 3 males) boars. We found that 9 pairs, out of 45 possible pairs, were most likely to be relatives. The result from kinship data and the location of the sampled hairs suggest that wild boars in this area live as family groups that consist of a mother and her offspring, which is consistent with known habits of wild boars. It is needed to include more samples and microsatellite markers for better precise estimation of kinship among the boar individuals.

• Korean Journal of Environmental Biology
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• v.36 no.3
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• pp.352-358
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• 2018

This study aimed to develop a species identification method for the egg and fry of the three Korean bitterling fishes (Pisces: Acheilognathinae), including Acheilognathus signifer, Acheilognathus yamatsutae and Rhodeus uyekii based on the PCR-based Restriction Fragment Length Polymorphism (RFLP) markers. We conducted a field survey on the Deokchicheon River from the North Han River basin, where the three Acheilognathinae species co-occur, and also analyzed the existing sequence dataset available from the GenBank. We found coexistence of the three species at the study site. The egg and fry were obtained from the host mussels (Unio douglasiae sinuolatus) by hand from May to June 2015 and in May 2017. To develop PCR-based RFLP markers for species identification of the three Acheilognathinae fish species, restriction enzymes pinpointing species-specific single nucleotide variation (SNV) sites in mitochondrial DNA COI (cytochrome oxidase I) and cyt b (cytochrome b) genes were determined. Genomic DNA was extracted from the egg and fry and RFLP experiments were carried out using restriction enzymes Apal I, Stu I and EcoR V for A. signifer, A. yamatsutae and R. uyekii, respectively. Consequently, unambiguous discrimination of the three species was possible, as could be seen in DNA band patterns from gel electrophoresis. Our developed PCR-based RFLP markers will be useful for the determination of the three species for the young and would assist in studying the spawning patterns and reproductive ecology of Acheilognathinae fishes. Furthermore, we believe the obtained information will be of importance for future maintenance, management and conservation of these natural and endangered species.