• Title/Summary/Keyword: 환경DNA

Search Result 965, Processing Time 0.031 seconds

Analysis of Vitellogenin Gene Expression in Synechogobius hastus (Gobiidae) (풀망둑 난황전구단백질 유전자발현 추적기법)

  • 계명찬
    • Korean Journal of Environmental Biology
    • /
    • v.22 no.1
    • /
    • pp.206-212
    • /
    • 2004
  • In an effort to develop the tools for monitoring the contamination of xenoestrogen in the aquatic environment of Korea, reverse transcription-polymerase chain reaction (RT-PCR) analysis of vitellogenin (VTG) mRNA expression were optimized in Synechogobius hastus. Based on the partial VTG cDNA sequence VTG mRNA level in livers from male fishes was analyzed by RT-PCR. As an internal control beta actin mRNA was amplified. 3 ${\mu}g$ of total RNA was reverse transcribed in 20 $\mu$l reaction using murine leukemia virus 〔MuLV〕 reverse transcriptase. Subsequent PCR using the 1 ${\mu}g$ of cDNA resulted in linear increase in PCR product of VTG in female liver cDNA from 10 to 30 cycles of amplification. On the contrary, in male, PCR product first detected at 28 cycles of amplification and linearly increased during 38 cycles of amplification, suggesting that male S. hastus expresses minute amount of VTG mRNA which is $2^{-18}$ equivalent of female. In conclusion, the optimized protocol of VTG mRNA expression in the liver of male S. hastus will be promising the environmental monitoring the xenoestrogen contamination in the western coast and estuaries in Korea.

Combined Genotoxic Effects of Aflatoxin B1, Ochratoxin A and Zearalenone in Rat Bone Marrow and Blood Leukocytes

  • Tigran, Harutyunyan;Anna, Karapetyan;Galina, Hovhannisyan;Rouben, Aroutiounian
    • Korean Journal of Environmental Biology
    • /
    • v.31 no.3
    • /
    • pp.189-191
    • /
    • 2013
  • Mycotoxins such as aflatoxin B1 (AFB1), ochratoxin A (OTA) and zearalenone (ZEA) are widespread contaminants of food and feedstuffs. It is very likely, that humans and animals are always exposed to mixtures of mycotoxins rather than to individual compounds. Therefore, risk assessments should consider mixture toxicity data. In the present study the combination of AFB1, OTA and ZEA was tested for genotoxicity in rat bone marrow and blood leukocytes after 15, 30 and 60 days treatment. The level of DNA damage was determined by the comet assay. The tail intensity and Olive tail moment in leukocytes and bone marrow cells were significantly higher than in controls. At the same time, the level of DNA damage in bone marrow cells was higher than in leukocytes. The data suggests that prolonged exposure to mycotoxins combination through food consumption can induce DNA damage contributing to the harmful effects in vivo.

Gender determination in parrots from Korean zoos using chromo-helicase-DNA binding protein 1 (CHD1) gene fragments

  • Kim, Jung-il;Do, Thinh Dinh;Choi, Tae-June;Yeo, Yonggu;Kim, Chang-Bae
    • Korean Journal of Environmental Biology
    • /
    • v.38 no.3
    • /
    • pp.350-354
    • /
    • 2020
  • Many parrots are considered endangered species due to threats from human activities. Gender determination is of great importance for biological studies and the conservation of endangered parrots. However, like other birds, gender determination in parrots is hindered due to the lack of external dimorphism between males and females. A molecular approach using the chromo-helicase-DNA binding protein 1 (CHD1) gene is commonly used for sexing birds. This study aimed to determine the gender of parrots from Korean zoos based on amplification and visualization of the partial CHD1 gene. The samples of 13 parrot species were collected from three different zoos in Korea and the extracted DNA templates were amplified using CHD1 gene primers. The gender of 27 samples of 13 species was determined by visualizing the PCR products on an agarose gel. While male parrots were indicated by a single band, female parrots were indicated by double bands. The findings provide additional information, which might be helpful for the management and care of parrots in Korean zoos.

Studies on Toxicological Evaluation of Freshwater Sediment using a PLHC-1 Cell Comet Assay (PLHC-1세포주의 Comet assay를 이용한 하천 퇴적토의 생태독성평가)

  • Bak, Jeong-Ah;Hwang, In-Young;Baek, Seung-Hong;Kim, Young-Sug
    • Korean Journal of Environmental Biology
    • /
    • v.29 no.1
    • /
    • pp.23-30
    • /
    • 2011
  • In this study, the Comet assay (evaluation of DNA damage) used the fish hepatocellular carinoma cell, PLHC-1, was tried to the sediment extract obtained from freshwater to understand its applicability as a tool for monitoring sediment toxicity. In parallel, induced EROD (7-ethoxyresorufin- O-deethylase) activity and DNA damage (TEM values) in PLHC-1 cells were measured for establishing the tandem endpoints of the PLHC-1cell test to test the ecotoxicity of sediment. Among several study sites in a small river passed through downtown and industrial park area, one of them, site B, showed a higher level of EROD activity and DNA damage than other sites. It indicates that a tandem endpoints of PLHC-1 cells could be useful tools for assessing the toxicity of sediment. The sensitivity of Comet assay with PLHC-1 cells was a little higher than that with a blood cell of frog tadpoles to the solvent extract of sediment. According to the results, a PLHC-1 cell-Comet assay could be used as a useful tool for evaluating ecotoxicity of the freshwater sediment. In addition, more detailed studies are needed to the contaminated site.

Current Status and Prospects for the Data Quality Control in Terms of Diatom Analysis (돌말류 분석 자료 정도 관리의 현황 및 전망)

  • KIM, HYESUK;KHIM, JONGSEONG;PARK, JINSOON
    • The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
    • /
    • v.26 no.3
    • /
    • pp.238-247
    • /
    • 2021
  • Diatoms are a type of microalgae which inhabits a wide variety of environments as one of the most important primary producers of freshwater and marine ecosystems. They have been widely used as bioindicators which represent the environmental characteristics, thus proper quality control of diatom data is very important to ensure for the researches from many scientists from various different regions to have scientific unity and objectivity. At present, diatom data analysis is primarily based both on morphological features and DNA sequences of given species. Challenge for the morphology-based analysis of diatoms is the consistent species identification among different taxonomists who interpret diatom community, while challenge for the molecular analysis of diatoms to secure reliable reference data. In the present study, we have reviewed the current status of data quality control of diatom analysis in Korea as well as the world. Finally, suggestions for the better data quality control for Korean marine diatoms have been also made.

1984~1985 미국 100대 발명, 발견

  • Korean Federation of Science and Technology Societies
    • The Science & Technology
    • /
    • v.19 no.2 s.201
    • /
    • pp.79-84
    • /
    • 1986
  • - 환경내의 유전물질 빈도를 모니터 하기 위한 DNA 탐지장치 - RRV1 및 RRV2 로보트 - 기능적 특성을 예견하기 위한 비천연 단백질의 설계와 합성 - 고분해능의 주사 이온 미세탐침 - 에크타켐 DT60 혈액분석기 - 뇌일혈 치료시스템 - 레일건 - 소프트스트립 - 고수준 핵폐기물 저장용 납-철-인산염 유리공정 - 단백질과 DNA의 합성 및 분석 - 물의 광전해용 전극 - 애버트 비루스용 고급휴지 - 3480 자기테이프 서브시스템

  • PDF

Constitution of evolution ecosystem for artificial microbes and its applications (인공미생물체를 위한 진화생태계의 구성과 그 응용)

  • Choo, Sung-Woo;Cho, Hwan-Kyu
    • Proceedings of the Korean Information Science Society Conference
    • /
    • 2005.07b
    • /
    • pp.268-270
    • /
    • 2005
  • 본 논문의 목적은, 간단한 DNA를 기반으로 서로 상호 작용하는 인공미생물체를 위한 진화생태계를 구성하는 것이다. 여기서, 강화 신호를 사용한 신경 회로망의 학습을 통해 인공미생물체의 지능 린 진화과정을 모방해서 자신의 DNA 및 주변 환경에 따라 행동 패턴이 변화하도록 하였다. 또한, 미생물의 진화론적 관점에서 생식 과정에서 두 개체산의 유전자 교환 등이 일어날 수 있도록 하였다. 그리고 이렇게 만들어진 진화생태계의 응용 가능성에 대해 다룬다.

  • PDF