• Journal of Environmental Health Sciences
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• v.32 no.6
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• pp.560-565
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• 2006

Extract of DNA for analysis of fragile X syndrome is usually performed by blood, the researches using hair root as specimen have been gradually spread. In this study, analyze fra X gene of the patients in mentally retarded children facilities was conducted using hair root DNA with molecular biologic test (PCR). The number of total subjects was 24, boys were 12, the average age was $17({\pm}3)$, and girls were 12, the average age was $18({\pm}2)$. In girls, normal size of band of 222 bp appeared in all lanes. Also, in all lanes except control in 517 bp, micro band appeared. Moreover, with appearance of band of 1198bp in lanes 2, 3, 4, 5, it is estimated that it is the band of full mutation whose CGG repeated sequences are more than 200. But it showed the peculiarity that it appeared with normal band in all the same lanes, thus it is not reasonable to judge it is the band of full mutation and further studies are needed. These results appeared in 50%, 6 of 12 mentally retarded girls. As the result of mentally retarded boys, normal band appeared in about 222 bp in control, however in experiment group, normal band did not appear. In 43%, 7 out of 12 boys, band did not either appeared in 1198bp, which showed different patterns from that of girls.

• The Journal of the Korea Contents Association
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• v.11 no.3
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• pp.65-72
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• 2011

We stand at real world that some practical use method of gene information appears in succession by entrance on the stage of advanced techonlogy. As a lot of studies and development are achieved based on analysis of bio data, necessity of a tool that can help correct interpretation of data is required more and more in a lot of targets of bioinformatics to search new relation and information are established. In this paper, we are offered in existing I wish to offer user a more convenient study tool developing system that can supplement shortcomings of various tools for data analysis. So we've designed to offer in united environment that is not environment that is parted ORF driving out, bio information retrieval and work of similarity comparison lamp to work for bio data analysis and offers lacking consecutiveness in existing analysis system.

• Korean Journal of Environmental Agriculture
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• v.34 no.4
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• pp.350-354
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• 2015

BACKGROUND: Botanical insecticides, especially Azadirachta Indica extract (AIE) and Sophorae radix extract (SRE) are widely used in Agriculture field. In our previous studies on genotoxicity test of AIE and SRE samples, a suspicious clastogenic properties was shown. Herein, we investigated the DNA damage effect of these botanical insecticide samples through the in vitro comet assay. METHODS AND RESULTS: Chinese hamster lung (CHL) fibroblast cell line was used, and methyl methanesulphonate was as positive control. Respective two samples of AIE and SRE were evaluated using Single Cell Gel Electrophoresis (Comet) assay and measured as the Olive tail moment (OTM). Results from this study indicated that all tested AIE and SRE samples did not show DNA damage in comet assay using CHL cells, compared with control. CONCLUSION: AIE and SRE samples used in this study were not cause genetic toxicity and are suitable for use as organic materials.

• Journal of Environmental Health Sciences
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• v.19 no.4
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• pp.59-66
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• 1993

The cellular toxicity and antitumor effects of bleomycin are thought to be occurred by formation of O$_2$-Fe$^{2+}$-bleomycin complexes that degrade DNA and release O$_2^-$ and $^{\cdot}$OH radicals. Hydroxyl radicals derived from hydrogen peroxide seem most likely to be involved in the various stages of carcinogenesis, and transition metals such as iron play a central role in activation of bleomycin and in formation of hydroxyl radicals. This study was performed to investigate whether treatment with ferrous sulfate increase chromosome aberration induced by bleomycin and hydrogen peroxide, and whether there is different repair mechanism for DNA damage induced by those chemicals. Treatment with 3AB, Ara C, during G$_1$ and post-treatment with caffeine, and Hu during G$_2$ increased the frequency of chromosome aberration induced by bleomycin but post-treatment with caffeine only did function that way when hydrogen peroxide was treated. When 6.6X 10$^{-7}$ M of bleomycin or 5.0X10$^{-5}$M of hydrogen peroxide were treated simultaneously with iron, the frequency of chromosome aberration was reduced, if compared with the results by bleomycin or hydrogen per oxide alone.

• Journal of the Korean Institute of Intelligent Systems
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• v.16 no.5
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• pp.556-561
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• 2006

It is very important to classify the DNA Chip image pattern in order to acquire useful information about genetic disease of people. In this paper, we developed the novel pattern classification method of DNA Chip image using MLP based back-propagation and Self organizing Map learning algorithm. And then we compared and analyzed these classified pattern results. Also we carried out experiment in the MV2440 board using CPU Cote for S3C2440(ARM 920T) and PC environment, and displayed its results in order to give the genetic information to user mote easily in various environment.

• Korean journal of applied entomology
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• v.60 no.2
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• pp.255-262
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• 2021

The potato tuber moth, Phthorimaea operculella (Zeller) has been known as a quarantine pest of potato. This study investigated inhibition doses of electron beam irradiation (EBM) by comparing their effects on the development and reproduction and DNA damage of the insect pest. Eggs (0-12 h old), larvae (3^rd and 5^th instar), pupae (less than 1 d old after pupation) and adults (less than 1 d old after emergence) were irradiated with increasing doses of EBM. The EBM with 150 Gy could not completely prevent the hatchability of eggs and pupation of the hatched larvae. The hatchability from the irradiated eggs were 19.3%. However, adult emergence from the irradiated eggs were completely inhibited. When 3^rd and 5^th instar larvae were irradiated at 100 Gy, the adult emergence from the irradiated larvae and the fecundity of the adults were completely inhibited. When pupae and adults were irradiated at 300 Gy and 400 Gy, respectively, the hatchability of the F₁ eggs was completely inhibited. The alkaline comet assay on the level of DNA damage by EBM in P. operculella adults indicates that the EBM increased DNA damage level in a dose-dependent manner, and the damage was repaired in a time-dependent manner. These results may recommend EBM of 150 Gy as a phytosanitary treatment for P. operculella. However further confirmative study is required for the practical application of this EBM dose for P. operculella disinfestation.

• Environmental Mutagens and Carcinogens
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• v.22 no.4
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• pp.248-254
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• 2002

Single cell gel electrophoresis assay was carried out to evaluate DNA damage in T-and B-lymphocytes from rats exposed to benzene and the correlation between DNA damage and the level of t,t-muconic acids, which are urinary benzene metabolites, was investigated. In control rats, the mean values of Olive tail moments in T-and B-lymphocytes were 1.507$\pm$0.187 and 1.579$\pm$0.206 respectively. DNA damages of T-lymphocytes in rats exposed for 4 weeks showed the highest Olive tail moments at each benzene concentration examined (2.72-4.351). However this DNA damage was decreased after 6 weeks of exposure (1.74-2.09). DNA damages of B-lymphocytes did not show such differences with exposure time or benzene concentration (1.49-2.07) except at 200 ppm at 4 weeks. T-lymphocytes show significantly more damages than B-lymphocyte upon acute exposure to benzene.

• Proceedings of the Korean Society of Soil and Groundwater Environment Conference
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• 2005.04a
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• pp.28-30
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• 2005

Because of high population diversity in soil microbial communities, it is difficult to accurately assess the capability of biodegradation of toxicant by microbes in soil and sediment. Identifying biodegradative microorganisms is an important step in designing and analyzing soil bioremediation. To remove non-important noise information, it is necessary to selectively enrich genomes of biodegradative microorganisms fromnon-biodegradative populations. For this purpose, a stable isotope probing (SIP) technique was applied in selectively harvesting the genomes of biphenyl-utilizing bacteria from soil microbial communities. Since many biphenyl-using microorganisms are responsible for aerobic PCB degradation In soil and sediments, biphenyl-utilizing bacteria were chosen as the target organisms. In soil microcosms, 13C-biphenyl was added as a selective carbon source for biphenyl users, According to $13C-CO_2$ analysis by GC-MS, 13C-biphenyl mineralization was detected after a 7-day of incubation. The heavy portion of DNA(13C-DNA) was separated from the light portion of DNA (12C-DNA) using equilibrium density gradient ultracentrifuge. Bacterial community structure in the 13C-DNAsample was analyzed by t-RFLP (terminal restriction fragment length polymorphism) method. The t-RFLP result demonstates that the use of SIP efficiently and selectively enriched the genomes of biphenyl degrading bacteria from non-degradative microbes. Furthermore, the bacterial diversity of biphenyl degrading populations was small enough for environmental genomes tools (metagenomics and DNA microarrays) to be used to detect functional (biphenyl degradation) genes from soil microbial communities, which may provide a significant progress in assessing microbial capability of PCB bioremediation in soil and groundwater.

• Journal of Environmental Health Sciences
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• v.28 no.1
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• pp.21-29
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• 2002

To identify and evaluate the dichlorobenzidine(DCB)-DNA adducts in liver cell and bladder epithelial cells by $^{32}$ P-postlabeling and GC/MS-SIM, we orally exposed the dichlorobenzidine(20mg/kh body wt./day) to male Sprague-Dawley rats(l85$\pm$10g) for 14 days. Two kinds of DCB-DNA adduct(A1 and A2) were found at the same site of thin layer chromatogram of $^{32}$ P-postlabeling method in liver cells and bladder epithelial cells. In liver cells, relative adduct labeling(RAL) $\times$ 10$^{12}$ of DCB-DNA adduct A1 were 34.1$\pm$3.71 and 69.9$\pm$5.02, that of adduct A2 were 74.1$\pm$10.1 and 105.1$\pm$10.1 on 10 and 14 days after treatment, respectively. And in bladder epithelia cells, RAL $\times$ 10$^{12}$ of DCB-DNA adduct A1 were 5.92$\pm$1.60 and 15.9$\pm$1.31, that of adduct A2 were 9.81$\pm$2.81 and 22.8$\pm$1.79 on 10 and 14 days after treatment, respectively. DCB metabolites formed DNA adducts were monoacetyl-dichlorobenzidine(acDCB) and diacetyl-dichlorobenzidine(di-acDCB), which was identify by gas chromatography/mass spectrometry-scan ionization mode(GC/MS-SIM), after hydrolysis of DCB-DNA adducts isolated from live cells and bladder epithelial cells. The base peak of acDCB were 252 and 294 m/z, and that of di-acDCB were 252, 294 and 336 m/z. In conclusion, the exposed DCB formed two kinds of DCB-DNA adduct, the proximate materials of that were acDCB and di-acDCB in liver and bladder epithelial cells. And the above GC/MS-SIM method was found the DCB-DNA adducts could be monitoring by gas chromatography.

• Proceedings of the Korea Environmental Mutagen Society Conference
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• 2002.11a
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• pp.60-66
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• 2002