• Title/Summary/Keyword: 확인조사

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한우의 개체별 체외수정란 생산

  • 임여정;정연길;석상현;박성백;송해범
    • Proceedings of the Korean Society of Developmental Biology Conference
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    • 2003.10a
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    • pp.139-139
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    • 2003
  • 최근, 한우의 수정란 체외이식두수가 급격히 늘어나고 있으나, 모축의 등록번호를 대부분 확인 할 수 없으며, 확인이 가능할지라도 개체별 수정란의 생산이 거의 이루어지지 않고 있다. 본 실험에서는 모축의 등록번호가 확인된 개체의 난소로부터 채취한 난포란만을 체외성숙, 수정, 배양하여 배 발생율을 조사하고, 동결성적을 조사하였다. 도축된 한우의 난소를 개체별로 채취하여 2$0^{\circ}C$생리식염수에 넣어 3시간 이내에 실험실로 운반하고, 난포란을 채취하였다 개체별로 채취한 난포란은 9~47개/난소였고, 평균 14.3개/난소였다. 개체별 난포란은 IVMD 101 (일본, 펩티트연구소)에서 22물24시간 동안 체외 성숙시킨 후, IVF 100(일본, 펩티트연구소)으로 2회 세정한 후, 각각의 배양액 50${\mu}\ell$ 소적에 5개씩 5~6시간 수정시켰다. 수정란을 저 산소 배지 IVD 101 (일본, 펩티트연구소)로 7~9일간 배양하여 배 발생율을 조사하고 동결하였다. 평균 수정율은 81%, 배반포의 발생율은 35% 였으며, 동결 가능한 수정란은 평균 4개/두 정도 얻을 수 있었다. 이상의 결과로 한우의 개체가 확인된 난소로부터 수정란생산이 가능하고, 육질등급이 우수한 한우의 수정란생산이 가능해졌으며, 앞으로 고 능력 젖소의 난소에서도 고능력 수정란의 생산이 가능할 것으로 사료된다.

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Room-Temperature Fabrication of Barium Titanate Thin Films by Aerosol Deposition Method (에어로졸데포지션법을 이용한 $BaTiO_3$ 박막의 상온 코팅)

  • Oh, Jong-Min;Nam, Song-Min
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 2008.11a
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    • pp.31-31
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    • 2008
  • 고주파 잡음 발생과 고집적화 문제 해결을 위해 고용량 디커플링 캐패시터를 기판에 내장하는 연구가 활발히 진행되고 있다. 본 연구에서는 초고주파 환경에서 고용량 기판 내장형 디커플링 캐패시터로의 응용을 위해 $BaTiO_3$박막을 에어로졸 데포지션 법을 이용하여 12~0.2 ${\mu}m$의 두께로 제조하였고 그 유전특성을 조사하였다. 그결과, 1 MHz에서 permittivity가 70, loss tangent은 3% 이하였으며, capacitance density는 $1{\mu}m$의 두께에서 59 nF/$cm^2$이었다. 하지만, 박막의 두께가 $1{\mu}m$ 이하에서는 XRD를 통해 결정성이 확인 되었음에도 큰 누설전류로 인해 유전특성을 확인할 수 없었다. 이 누설전류의 발생 원인을 조사하기 위해 $BaTiO_3$박막의 표면의 미세구조를 SEM으로 관찰한 결과 여러 결함들이 확인되었으며, 또한 전극 직경의 크기를 1.5 mm에서 0.33 mm로 작게 변화시킴으로서 그 유전특성을 조사하여 박막의 불균일성과 박막화의 가능성을 확인하였다.

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Report on the Eradication of Nutria (Myocastor coypus Molina, 1782), an Invasive Alien Species, from Jeju-do, South Korea - Case of Songdang-ri, Jeju-si - (제주도 침입외래생물 Nutria (Mycastor coypus Molina, 1782)의 퇴치 사례 보고 - 제주시 송당지역의 사례 -)

  • Ga-Ram Kim;Jun-Won Lee;Seon-Mi Park;Sung-Hwan Choi;Young-Hun Jung;Hong-Shik Oh
    • Korean Journal of Environment and Ecology
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    • v.36 no.6
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    • pp.582-591
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    • 2022
  • This study was conducted to eliminate Myocastor coypusMolina, 1782 (Nutria) from Jeju Special Self-Governing Province, South Korea. Habitat identification and eradication were carried out from September to November 2013, and a survey was carried out until June 2022 to check whether the eradication was successful. The habitat was identified with unmanned cameras, interviews, and literature surveys, and the capture was performed using the trapping method with food to attract nutria to the habitat area. The study area for the follow-up survey, which was set relatively wide considering nutria's home range, included wetlands and rivers within 4.0 km2 of the habitat area (eradication area). As a result, nutria's habitat was confirmed only at Songdang Ranch, Songdang-ri, of Jeju Island, with traces of habitat (footprints, excrement, and burrows) confirmed in waterways and ponds within the pasture. Eight individuals were captured, including four females, three males, and one individual in too advanced a state of decay to identify the sex. The follow-up survey thoroughly investigated the habitat and its surroundings, focusing on three areas with permanent water, Seongeup Reservoir, Cheonmi Creek, and Molsuni Pond, but no signs of habitat were identified. Therefore, it is determined that nutria inhabiting Jeju Island has been completely eradicated. It is believed that the successful eradication of nutria in the Jeju Special Autonomous Region was possible due to a synergy between 1) the eradication of nutria at the beginning of the settlement phase through rapid capture after confirming the nutria habitat and 2) the delayed expansion period because of rare presence of wetlands, where water is constantly stagnant, on Jeju Island. These results imply that quickly identifying the ecological characteristics of the species and preventing disturbances before they or at the beginning of the ecological disturbance through control and eradication at the initial stage of settlement before the expansion stage is an effective measure to cope with the influx of alien species.

인터넷사용자측정 조사에서의 표집틀의 문제

  • 장원호;박동선
    • Proceedings of the Korean Association for Survey Research Conference
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    • 2000.11a
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    • pp.129-140
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    • 2000
  • 인터넷 사용자가 과연 어떤 사이트를 주로 이용하고 그 이용 시간은 어떠한 수준인지를 확인하고 더 나아가서 각 사이트 이용자들은 어떠한 특성을 나타내고 있는지를 알아보는 인터넷사용자측정(Audience-centric Measurement)의 중요성은 인터넷 조사의 기반이 되는 데이터를 제공한다는 점뿐 아니라 최근 활발히 연구되고 있는 데이터마이닝(Data Mining)과 그것을 바탕으로 하는 CRM(Customer Relationship Management)의 기초자료로서 그 중요성이 날로 커지고 있다. 본 연구는 기존의 인터넷사용자측정 조사의 국내외 연구 동향과 그 차이점을 살펴보고, 국내의 기존조사가 가지고 있는 문제점, 특히 조사의 표집틀의 왜곡과 관련된 문제점을 분석하고 이를 해결하기 위한 실천적, 이론적 대안을 제시하고 있다.

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Flora of Western part in Korea Civilian Control Zone (CCZ). (서부 민통선 일원의 관속식물상)

  • Kim, Kyoung-Hoon;Kang, Shin-Ho
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2019.04a
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    • pp.31-31
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    • 2019
  • 서부 민통지역은 육군 제 1사단, 육군 제 25사단, 육군 제 28사단 관할지역에 걸쳐있다. 경기도 파주시 민통지역은 육군 제1사단 관할지역으로 장단면, 군내면, 진동면을 포함한다. 연천군 반정리는 육군 제 25사단 관할 지역 내에 있다. 본 조사에서는 장단면 일대와 군내면 정자리 덕진산성, 진동면 동파리 일월봉, 임진강변, 수내천, 세월천을 중심으로 세 개 면과 연천군 장남면 반정리의 식물상을 조사하여, 서부 민통지역의 주요 식물들의 분포를 자세히 파악하고자 하였다. 2012년 4월부터 2018년 10월까지 조사를 통해 463점의 표본을 확보하였고, 총 96과 307속 415종, 3아종, 45변종 6품종의 469분류군이 확인되었다. 조사 시기 중 멸종위기종은 발견되지 않았으며, 우리나라 특산식물로는 고려엉겅퀴, 벌개미취, 키버들 등 7분류군이 확인 되었다. 식물구계학적 특정종으로는 Ⅴ등급 통발을 비롯, IV등급 8종, III등급 11종, II등급 11종, I등급 16종이 발견되었다. 서부 민통지역에서 확인된 외래 및 귀화식물은 단풍잎돼지풀을 포함한 35분류군으로 전체 식물의 7.5%에 해당한다. 최근 무분별한 개발로 인한 민통지역 자연자원의 보전대책 수립도 요구된다.

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Infrared Irradiation and Conservation Treatment of Wooden Fish Excavated from Wiryeseong Fortress on Seonggeosan Mountain in Cheonan (천안 성거산 위례성 출토 목어의 적외선 조사 및 보존처리)

  • Jo, Sangyoon;Kim, Soochul
    • Conservation Science in Museum
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    • v.26
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    • pp.13-24
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    • 2021
  • Two wooden fish excavated from Wiryeseong Fortress on Seonggeosan Mountain in Cheonan-si, Chungcheongnam-do Province were frail due to their deterioration within the burial environment. To prevent further deformation, conservation treatment was conducted on them to strengthen the wooden materials and stabilize the dimensions. Prior to the conservation treatment, the species of wood was determined to be pine and infrared Irradiation on the patterns remaining on the wooden fish revealed that they were depictions of carp. For conservation treatment, the wooden fish were impregnated with PEG #3, 350 in a water solution with a 40% concentration, followed by freeze-drying.

Physicochemical Quality and Luminescence Characteristics of Gamma-irradiated Dried Fish Products (감마선 조사에 따른 건포류의 이화학적 품질 및 발광 특성)

  • Park, Eun-Joo;Jang, Han-Na;Jo, Deokjo;Kim, Gui-Ran;Kwon, Joong-Ho
    • Korean Journal of Food Science and Technology
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    • v.45 no.2
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    • pp.167-173
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    • 2013
  • Dried fish products, such as seasoned cuttlefish, anchovy, and filefish, were analyzed to investigate the physicochemical quality and luminescence properties following gamma-irradiation. The overall color difference slightly increased in all irradiated samples, showing significant changes in Hunter's L, a, and b color values. Cuttlefish and filefish showed higher values in hydrophilic browning, whereas anchovy showed a higher value in lipophilic browning. The brown color intensity of filefish showed a dose-dependent increase. The thiobarbituric acid (TBA) value significantly increased in irradiated samples of cuttlefish and anchovy, which have relatively high lipid contents. Irradiated samples, except anchovy, were not suitable for screening using photostimulated luminescence (PSL) because only negative or intermediate values were observed. Thermoluminescence (TL) measurements were suitable for all fish samples, i.e., irradiation-specific glow curves were obtained, signal intensity increased, and the TL ratio fulfilled the criterion. There was no significant difference among the irradiated samples with regard to the sensory properties.

DNA Repair Characteristics of MRC-5 and SK-N-SH Irradiated with Proton Beam (양성자빔 조사에 따른 MRC-5와 SK-N-SH의 DNA 손상 후 회복 특성)

  • Choi, Eun-Ae;Lee, Bong-Soo;Cho, Young-Ho
    • Journal of radiological science and technology
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    • v.34 no.4
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    • pp.333-339
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    • 2011
  • The purpose of this study is to compare DNA repair characteristics of normal fibroblast cell (MRC-5) and neuroblastoma cell (SK-N-SH) induced by proton beam. Cells were irradiated with 2Gy, 5Gy and 8Gy proton beam. The rate of DNA rejoining was measured by alkaline version of the comet assay. After a repair time, tail moment was measured again. The tail moment of MRC-5 was lower than SK-N-SH. However, after 8Gy of exposure, the tail moment of MRC-5 was measured as 50.320223.17155 which represents dangerous level of DNA damage. The cells were repaired practically within 25 hours after 2 and 5Gy of exposure while they were not fully recovered after 8Gy of exposure. Especially, tail moment of MRC-5 after 25 hours was 18.15364.42849. In the distal declining edge of SOBP, the RBE value is increased by high LET. The RBE differences of SOBP in high-dose were greater than low-dose. After the high-dose exposure, MRC-5 of normal fibroblast cell could lead to lasting DNA damage as shown in this study. In conclusion, we has to pay special attention when the region of the treatment volume is close to sensitive tissues.

Photostimulated Luminescence-Thermoluminescence Application to Detection of Irradiated White Ginseng Powder (방사선 조사 백삼분말의 PSL-TL 다중검지법)

  • Chung, Hyung-Wook;Delincee, Henry;Kwon, Joong-Ho
    • Korean Journal of Food Science and Technology
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    • v.32 no.2
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    • pp.265-270
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    • 2000
  • White ginseng powder, permitted to be irradiated in Korea for the purpose of microbial decontamination, was treated with electron beam at doses of $0{\sim}15\;kGy$ for a detection trial whether it is irradiated or not by measuring photostimulated luminescence for whole samples first and then (TL) for the mineral adhering to the samples. PSL values were less than threshold value (700, $T_{1}$) and were negative for nonirradiated samples but more than 5000 $(T_2)$ and were positive for irradiated ones. After PSL measurement mineral was separated from the whole samples using density separation. Mineral of nonirradiated samples was characterized by glow curves which have low intensity and were situated at the high temperature region (about $300^{\circ}C$) by the low level of natural radioactivity. Glow curves of minerals for all irradiated samples were observed at about $200^{\circ}C$. TL ratio by normalization was 0.01 for nonirradiated sample and more than 0.78 for irradiated samples, and it was possible to detect whether white ginseng powders were irradiated or not.

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Analysis of the Volatile Flavor Components in Plum ($Prunus$ $salicina$) Irradiated with an Electron Beam (전자선 조사한 자두의 휘발성 유기성분 변화)

  • Jeong, In-Seon;Lee, Sun-Im;Jeon, Dong-Bok;Hong, Young-Sin;Kim, Jae-Sung;Choi, Sung-Hwa;Nho, Eun-Yeong;Choi, Ji-Yeon;Kim, Byung-Sook;Kim, Kyong-Su
    • Food Science and Preservation
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    • v.19 no.2
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    • pp.249-256
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    • 2012
  • The changes in the volatile organic compounds in plum after its electron beam irradiation and storage were determined using the simultaneous distillation extraction method and gas chromatograph-mass spectrometry. There were 44, 46, 45, 47, and 38 volatile compounds in the 0-, 0.25-, 0.5-, 0.75-, and 1 kGy irradiated samples, respectively. Also, the volatile flavor components of the plum that was stored for 30 days were identified as 48, 40, 40, 39, and 40 components. The compositions of the volatile compounds of the control and irradiated samples showed a similarity after the storage. Especially, the more important volatile flavor of the plum was identified as hexanal of the C6compounds, (E)-2-hexenal and (Z)-3-hexenal. In particular, hexanal, (E)-2-hexenal, and (Z)-3-hexen-1-ol increased in all the doses, where as hexanol and (E)-2-hexen-1-ol decreased. Among the lactone compounds, ${\gamma}$-hexalactone, ${\gamma}$-octalactone, and ${\gamma}$-decalactone were identified during the storage period in the raw samples. Hexanonic acid and 2-hexenoic acid were not identified during the storage of the samples, and 2-methylprrole was detected only when the storage samples were irradiated at a dose higher than 0.5kGy. Therefore, it was shown that there was no effect on the variation of the volatile organic component suntil 1 kGy in the plum was irradiated with an electron beam.