• Korean Journal of Microbiology
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• v.55 no.1
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• pp.69-73
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• 2019

During screening of microbes for compounds having cosmetic benefits, we isolated Marinobacter salarius HL2708#2 from lava seawater on Jeju Island, Republic of Korea. The complete genome sequence was determined. Strain HL27080#2 features a circular chromosome of 4,304,603 bp with 57.21% G+C content and a 244,163 bp plasmid with 53.14% G+C. There were 4,180 protein coding sequences identified, along with 49 transfer RNA and 18 ribosomal RNA noncoding genes. The genome harbored genes for the utilization of alcohol, maltose/starch, and monosaccharide as sole carbon sources. Genes responsible for halophilic characteristics and heavy metal resistance could be annotated, as well as aromatic and alkane hydrocarbons. Contrary to the prior report that M. salarius is negative for nitrate and nitrite reduction, nitrate/nitrite reductase along with nitrate/nitrate transporters and nitronate monooxygenase were evident, suggesting that strain HL2708#2 may be able to denitrify extracellular nitroalkenes to ammonia.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.22 no.2
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• pp.638-643
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• 2021

Oil is used in various industries, including the agricultural sector, food industry, and functional cosmetics. These oils are chemically unstable and prone to oxidation when exposed to oxygen, light, moisture, or high temperatures. Therefore, various attempts have been made to encapsulate them so that they are not exposed to such environments. When oil is injected into a refrigerant with greater density, the oil can be encapsulated as it rises due to buoyancy caused by the density difference. In this study, oil encapsulation was simulated to find the optimal conditions for operating equipment using computational fluid dynamics (CFD) for multiphase flows. Water or serum can be used as a refrigerant. The viscosity of water is relatively small, and if it is used as a refrigerant, oil droplets can be produced well even if oil and water are continuously injected in the equipment. However, the viscosity of serum is very high, and if it is used, the oil is stretched out and does not leave the nozzle. The results show that when using serum as a cooling medium, oil encapsulation is possible if the injection is stopped for some time after instantaneous injection at high speed.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.22 no.2
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• pp.745-754
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• 2021

The purpose of this study was to evaluate and verify the effectiveness of sustainable cosmetic raw materials developed from Gynostemma pentaphyllum, a plant native to Ulleungdo, in improving the skin barrier function and treating atopic dermatitis. Cells were derived from adult Gynostemma pentaphyllum plants, and suitable conditions for mass culture of the cells were established in a bioreactor. DNA components and amino acids extracted from this mass culture were identified from the HPLC fraction. In the in vitro efficacy evaluation results, changes in the expression levels of skin barrier-related proteins such as filaggrin (FLG) and Zonula occludens-1 (Zo-1) were insignificant. It was confirmed that the expression levels of the proteins thymic stromal lymphopoietin (TSLP) and interleukin-33 (IL-33) were significantly reduced. These results lead to the conclusion that Gynostemma pentaphyllum cell extracts have significant anti-inflammatory effects and that these extracts can be widely used as sustainable, nature-friendly active material in cosmetics with anti-inflammatory effects and targeted at improving atopic dermatitis.They may find use in anti-aging cosmetic products as well.

• Journal of the Korean Applied Science and Technology
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• v.38 no.1
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• pp.217-227
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• 2021

In this study, Korean-cultivated Houttuynia cordata Thunb. (HC) was extracted anti-inflammatory effects of extracts were compared in order to confirm the possibility of natural cosmetics as a raw material. HPLC pattern analysis, cell viability assay, total phenolic contents, ABTS/DPPH radical scavenging analysis, measurement of ROS production, griess reagent assay, and luminex technique. The content of quercetin was higher in fresh Houttuynia cordata (HC-F) than in dried Houttuynia cordata (HC-D). Also, content of HC extracts shows a difference in the content of the two representative compounds (polyphenol and flavonoid) according to the stored differently. ROS production showed higher inhibitory rates at HC-F. The anti-inflammatory activity of HC-D was shown higher than that of HC-F. But, in the measurement of the reduction in inflammatory cytokines, IL-1β showed that HC-D had a relatively high reduction effect, and IL-6 and TNF-α showed a high reduction effect. And these results will be a basis for the development of cosmetology in skin care and improvement of skin problem.

• Journal of the Korean Applied Science and Technology
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• v.38 no.6
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• pp.1524-1532
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• 2021

In this study, Gomchwi (Ligularia fischen) derived from Taebaek-si, Gangwon-do was extracted with 70% ethanol (LFE) and antioxidant activity was measured. The following experimental techniques were used to evaluate the antioxidant efficacy of LFE. Total phenolic contents, ABTS/DPPH radical scavenging analysis, cell viability assay, NO assay, and quantitative real-time PCR technique. The content of polyphenol and flavonoid was each 113.97±0.37 mg GAE/g or 29.22±2.06 mg QE/g in LFE. DPPH radical scavenging activity was measured to be 25 ㎍/㎖ 11.26±0.95%, 50 ㎍/㎖ 17.12±0.63%, 100 ㎍/㎖ 29.54±0.36%, 250 ㎍/㎖ 68.31±0.28%, 500 ㎍/㎖ 75.12±0.05%, and 1000 ㎍/㎖ 75.75±1.57%. In addition, ABTS radical scavenging activity was identified as LFE 25 ㎍/㎖ 13.75±0.21%, 50 ㎍/㎖ 26.71±0.20%, 100 ㎍/㎖ 56.92±0.22%, 250 ㎍/㎖ 91.30±0.12%, 500 ㎍/㎖ 93.40±0.02, and 1000 ㎍/㎖ 93.19±0.04%. There was no significant cytotoxicity of LFE. NO production was significantly decreased to LFE 50 ㎍/㎖ 79.40±2.64%, 100 ㎍/㎖ 55.01±5.36%, and 200 ㎍/㎖ 30.93±3.11%. Also, the NOS2 gene expression was significantly reduced to LFE 50 ㎍/㎖ 0.94±0.11, 100 ㎍/㎖ 0.59±0.05, and 200 ㎍/㎖ 0.32±0.04. This result objectively confirmed the antioxidant effect of Gomchwi. We will continue to conduct in-depth research. Therefore, it is believed that the possibility of using Gomchwi as a cosmetic and functional food material can be established.

• Journal of Naturopathy
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• v.10 no.2
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• pp.108-113
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• 2021

Background: Results on the action of antioxidants in extracts of Equisetum hyemale stems and roots have already been reported, but the antioxidant properties have not been analyzed. Purpose: This study was to determine the molecular structure of antioxidants in substances extracted from E. hyemale stems and roots. Methods: Component analysis was analyzed by HPLC and LC-MS after extraction with hot water and ethyl alcohol. Results: The HPLC chromatogram of stem and root extracts showed four significant peaks at a wavelength of 205 nm. Peak 1 at 280 nm is a typical simple phenolic type, and both peaks 2-4 near 280 nm and 370 nm are typical flavonoid glycosides. As for the antioxidant level of the extract by HPLC analysis, the sum of the peaks at 740 nm was the highest at 3,669 mAU in the 100% ethanol extract, 3,096 mAU in the 70% ethanol extract, and 2,868 mAU in the hot water extract. As a result of LC-MS analysis of the antioxidant extract, kaempferol-3-sophoroside-7-glucoside with a molecular weight of 772 da at peak 3, and kaempferol-3-sophoroside-8-glucoside with a molecular weight of 788 and 772 at peak 4 was identified. Conclusions: The above results show that two types of antioxidants were identified in the antioxidant extract of E. hyemale exrtracts. Therefore, the potential as a raw material for functional cosmetics has increased.

• Journal of Naturopathy
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• v.10 no.1
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• pp.42-47
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• 2021

Purpose: This study investigated the antioxidant activities of water extracts from Hibiscus petals, Moringa oleifera gymnosperm, and Nipa fruticans wurmb. Also, the possibility of their use as a functional cosmetic material and food were searched. Methods: We extracted Hibiscus petals, M. oleifera gymnosperm, and N. fruticans wurmb with water. And then, we measured the content of total polyphenols and flavonoids and the ability to scavenging free groups of ABTS and DPPH to study the antioxidant function. The toxicity of samples evaluated by measuring cell viability. Results: The polyphenol content of the water extract of N. fruticans wurmb was 109 ㎍/mg, which was significantly higher than that of Hibiscus petals (13 ㎍/mg) and M. oleifera gymnosperm (19 ㎍/mg). Radical scavenging ability was also excellent in N. fruticans wurmb, and the cytotoxicity test results of the samples were similar. Conclusions: The water extracts showed antioxidant activity to use for cosmetic materials or natural healing foodstuff.

• Journal of Life Science
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• v.31 no.7
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• pp.654-661
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• 2021

In this study, in order to isolate excellent whitening agents from fungal cultural broth, various fungi were collected from wild areas in South Korea and then screened for tyrosinase inhibition activity, as tyrosinase is a precursor for the biosynthesis of melanin in regulating skin color. A fungus strain that inhibits tyrosinase activity has been identified and confirmed as Trichoderma viridescens (later renamed T. viridescens SW-1) via ITS sequencing. In T. viridescens SW-1, tyrosinase inhibitory activity was strongest on day three of culture. A 5% culture broth showed a tyrosinase inhibitory activity greater than 90% and exhibited high thermostability on day three. At 10% culture broth, the accumulations of intra- and extracellular melanin were inhibited above 27.1% and 7.5%, respectively. In summary, the physical and functional properties of the tyrosinase inhibitory substances of T. viridescens SW-1 included high levels of inhibition of melanin synthesis and antioxidative activity as well as thermostability. Therefore, we suggest that the whitening substance identified from the cultural broth of T. viridescens SW-1 has potential for application as a functional cosmetic ingredient.

• Journal of Life Science
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• v.31 no.11
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• pp.1004-1009
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• 2021

Angelica gigas Nakai (AGN) has been used in Korean herbal medicine for various pharmacological activities, such as to create antioxidant and skin whitening effects. Decursin and decursinol angelate of AGN extracts can be used as potential active drugs and cosmetic ingredients. This study investigated the possibility of topical delivery of AGN extracts using a manufactured emulsion system. Lotion was formulated by using Tefose^® and paraffin for the oil phase, Kolliphor RH 40 for the surfactant and solubilizing agent-which showed high solubility in water (0.82 mg/ml)-and a water phase with a carbomer. In vitro skin permeation of decursin and decursinol angelate was determined using a Strat-M^® membrane in Franz diffusion cells. Lotion samples as the experimental group (248.08±19.72 ug/cm²) significantly increased the permeation of decursin and decursinol angelate for up to 24 hr compared to the control group (119.18±19.23 ug/cm²). The permeability was also characterized by the flux (penetration rates) and Kp (permeability coefficient) values. The experimental group (17.20±1.23 ug/h/cm² and 5.73±1.39 cm/h^*10^-3) had higher flux and Kp than the control group (8.22±1.24 ug/h/cm² and 2.74±0.51 cm/h^*10^-3). Lotion with decursin and decursinol angelate of AGN extracts could be used for the topical application of drug and cosmetic products.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.49 no.1
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• pp.75-85
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• 2023

This study was conducted to assess the effect of heptapeptide, composed of seven amino acids, on the activation of human hair cells isolated from human hair follicles. We have confirmed that the heptapeptide could bind to Lgr5 from the results of surface plasmon resonance (SPR) analysis. Heptapeptide enhanced the proliferation of human hair follicle dermal papilla cells (HHFDPCs) in a dose dependent manner. It induced the protein level of nuclear β-catenin, and the expressions of β-catenin downstream target genes, including LEF1, Cyc-D1 and c-Myc, in HHFDPCs. Heptapeptide significantly induced the phosphorylation of Akt and ERK, and the mRNA expressions of growth factors, including hepatocyte growth factor (HGF), keratinocyte growth factor (KGF) and vascular endothelial growth factor (VEGF), in HHFDPCs. In addition, heptapeptide significantly increased mRNA expression levels of differentiation-related transcription factors of human hair germinal matrix cells (HHGMCs) and differentiation markers of human hair outer root sheath cells (HHORSCs). Additionally, we investigated the effect of heptapeptide on human hair follicle stem cells (HHFSCs) differentiation and found that the heptapeptide reduced the mRNA and protein levels of stem cell markers, while it increased those levels of differentiation markers. These results have indicated that the heptapeptide promotes proliferation or differentiation of various types of hair follicle constituent cells through the induction of Wnt/β-catenin signaling. From the results, we have suggested that the heptapeptide in this study could be applied as a new functional material for the improvement of hair growth and alopecia.