• Korean Journal of Medicinal Crop Science
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• v.1 no.2
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• pp.137-157
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• 1993

This study was carried out to investigate the optimal condition for multiple propagation through leaf tissue culture and to apply anther culture techniques to Pulsatilla koreana Nakai breeding. Leaf and anther of Pulsatilla koreana Nakai were cultured on MS, MT, LS and $B_5$ media supplemented with several growth regulators and nitrogen sources under various conditions. For callus induction and differentiation from the Pulsatilla koreana leaf segments were more effective in the combination of zeatin and auxin than auxin alone. The color of the callus was green when treated with IBA alone. Shoot differentiation was more effective when treated with zeatin than auxin alone, especially the best hormoal combination for shoot differentiation was zeatin 1.0mg/l +NAA 0.1mg/l, while 2,4-D inhibited shoot differentiation. The appeared rate of S pollen was 35% in vivo, while that of S pollen by low temperature$(4^{\circ}C)$ pretreatment for 4 days was increased by 53% and the optimum culture time for callus induction from anther was uni-nucleate stage. $B_5$ basal medium supplemented with NAA 0.5mg/l and zeatin 1 mg/l was the most effective on callus formation and the best results of plant regeneration were obtained from combination of NAA 0.5mg/l and zeatin 0.5mg/l in anther culture. $NH_{4}NO_3$ as more effectives as the nitrogen source than $KNO_3$ and the combination with zeatin 2.0mg /L was the best effective. The best combination for plant regeneration in callus induced from anther was $NH_{4}NO_3$ 1650mg/l + $KNO_3$ 3800mg/l + zeatin 2.0mg/l. Ploidy level of anther-derived plants appeared 28% haploid, 47% diploid and the others were triploid, tetraploid and mixploid. In compare with E.S.T, M.D.H and P.X banding patterns were distinguished among callus, haploid and diploid plants in electrophoresis.

• Korean Journal of Food Science and Technology
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• v.3 no.3
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• pp.144-149
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• 1971

The sensitive technique of activation analysis is well suited for this study since the elements such as As, Br, and Se in tobaccoes are expected to be high concentration. As, Br, and Hg were determined by Bethge destruction method and subsequent neutron activation analysis. $^{77m}Se$ was also by non-destruction activation analysis. The quantities of the element determined in Korean tobaccoes are given as follows in ppm: As, 0.65 ppm. Hg, 0.74 ppm. Se, 1.18 ppm. Br, 7.1 ppm. From the date given it seems that Korean tobaccoes and foreign tobaccoes contained considerably high concentration of selenium and mercury.

• Journal of Conservation Science
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• v.26 no.4
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• pp.459-465
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• 2010

The 5th century kiln that produced pottery coffins was found for the first time in Korea, located in the Oryang-dong remains in the city of Naju. This kiln, located in the central area of the Yeongsan River, provides important data for research on mortuary practices in prehistoric society, including the structure of production and patterns of distribution and consumption. For this study, the remains of five ancient tombs and pottery coffins excavated from the 4th century Mandong archaeological site were chosen to determine the area of consumption of pottery coffins produced at the Oryang-dong kiln. The samples from each area of remains were analyzed for minor elements using neutron activation analysis method, and from these results, the identities of the corresponding production areas were investigated using the multi-variant statistical analysis of discriminant analysis. The evidence strongly suggests that pottery coffins produced at the Oryang-dong kiln in Naju were used in ancient tombs of the Bannam mound in Naju, the Okyari mound in Yeongam, and the Banam mound in Hampyeong, reaching those sites through trade and distribution. The findings also suggest that pottery coffins from the Mandong archeological site in Gochang and the Inpyeong mound in Muan were not produced at the Oryang-dong kiln in Naju, but rather were brought from pottery kilns in different production areas, through trade and distribution.

• Proceedings of the Korea Water Resources Association Conference
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• 2018.05a
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• pp.382-382
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• 2018

최근 기후변화 및 도시화로 인해 집중호우와 도시 내의 불투수층이 증가하고 있으며, 현재 도심의 강우관리시스템이 한계에 달하여 수재해 피해가 급증하고 있다. 이와 같은 문제를 해결하기 위한 방안으로 저영향개발 기법이 대두되고 있으며, 본 기법은 해당 지역의 강우를 분산식 빗물처리 하여 도시 내의 수문학적 특성을 개발이전 상태로 회복 및 유지하는 기술이다. 저영향개발 기법의 종류는 투수성 포장, 옥상녹화, 빗물정원, 식생저류지, 식생화분 등이 있다. 본 연구에서는 저영향개발 기법 중 하나인 투수성 아스팔트에 대한 우수저감 효과를 분석하기 위해 경상남도 양산시 부산대학교 양산캠퍼스 그린인프라 저영향개발 센터에서 주차장형 LID 시설의 투수성 아스팔트 포장과 대조군인 불투수 콘크리트 포장을 실험대상으로 선정하였다. 포장체는 $10.85m(L){\times}2.3m(B){\times}0.9 m(H)$의 크기로 원지반 침투가 없는 박스형 실험시설로써 이동형 강우모의장치를 이용하여 인공강우실험을 실시하였다. 강우모의 시에 발생하는 지표유출 및 침투유출은 각 유공관을 통해 모니터링 박스에 차집되어 실시간으로 웹사이트 상에 저장된다. 저장된 자료를 이용하여 투수성아스팔트의 첨두유출량 저감, 지체시간지연효과를 분석하였다. 분석된 결과를 통해 첨두유출량은 11%저감되었고 투수성 아스팔트와 불투수 콘크리트는 각각 3시간, 1시간 동안 유출되어 2시간의 차이를 보여준다.

• Proceedings of the Korea Water Resources Association Conference
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• 2020.06a
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• pp.205-205
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• 2020

도시화로 인해 불투수층의 증가는 강우시 유출을 증가시켜 물순환을 왜곡시키고 다양한 오염물질을 수계로 배출시켜 수질악화 및 수생태계를 훼손시킨다. 이러한 환경 수문학적 문제를 해결하기 위하여 침투, 여과, 저류 등의 물리적 기작, 식생 및 토양의 생물학적 기작을 이용한 LID 기술을 적용하고 있다. LID 시설에 적용되는 식물은 심미적, 경관성을 증대시켜주나, 다량의 오염물질이 유입될 경우 일부 식물의 고사를 유발한다. 따라서 본 연구는 LID 시설내 식물 체내 중금속 농도를 파악하고자 수행하였다. 본 연구는 지붕 강우유출수를 처리를 위해 설치된 LID 시설(침투화분) 모니터링 대상 지점으로 선정하였으며, 패랭이(Dianthus chinensis)와 조팝나무(Spiraea fritschiana)가 식재되어있다. LID 시설 내 식재된 식물과의 비교를 위해 패랭이와 조팝나무가 식재된 화단을 각각 대조군으로 선정하였다. 패랭이는 잎과 뿌리, 조팝나무는 줄기와 잔가지로 구분하여 체내 중금속 함량에 대해 분석을 수행하였다. 중금속 분석은 토질오염공정시험기준에 의해 Fe, Ni, Zn, As를 분석하였다. 연구결과, LID 시설에 적용된 식생과 대조군 식생의 체내 Fe 농도는 패랭이(1176.9~3804.6mg/kg), 조팝나무(218.4~342.5mg/kg)이며, Ni의 경우 패랭이(13.3~21.4mg/kg), 조팝나무(7.2~12.5mg/kg) Zn은 패랭이(33.6~160.5mg/kg), 조팝나무(46.1~243.2mg/kg), As는 패랭이(7.3~37.3mg/kg), 조팝나무(1.9~8.7mg/kg)로 대조군에 비해 유사하거나 높은 값을 보이는 것으로 분석되었다. 또한, LID 시설에 식재된 조팝나무는 대조군에 비해 줄기는 약 26배, 잔가지 19배 이상 높은 농도를 보이는 것으로 나타났으나, 중금속 농도의 차이는 식물의 중금속 흡수 혹은 토양의 중금속 흡착능력이 토양의 종류, 특성에 따라 다를 수 있기 때문에 모니터링 환경에 따라 편차가 존재할 것으로 판단된다.

• Journal of Conservation Science
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• v.35 no.6
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• pp.573-587
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• 2019

In this study, reproducing specimens were made from mixing domestically produced magnetite, clay and non-plastic raw materials to reproduce the pigments used in the manufacture of traditional cheolhwa buncheong stoneware. In order to reveal the color fomation of glaze, 30 specimens with good color development were analyzed scientifically. Magnetite, which is the main raw material of the pigment, is a pigment capable of creating a dark black color in a reducing environment at 1,200℃. However, it reacts with the additionally added lime component and discolors to greenish yellow color in oxidizing environment at 1,230℃. Hematite is not significantly affected by the firing temperature and environment, but develops a dark black color when mixed with clay with iron content of more than 10%. The fluidity of the pigment is determined by R₂O₃/RO₂ value, which also affects the color development. In the microtexture observation, the color formation of the glaze layer and the iron oxide crystals identified some differences depending on the particle size of the pigment and the firing environment. Reproduced specimens made of magnetite are present in the form of aggregates of iron oxide in the interface between glaze layer and slip layer in the oxidizing environment at 1,200℃. However, in the reducing environment, aggregates of iron oxides do not exist in the reproduced specimens, and they are homogeneously distributed in the glaze layer and formed a dark black color. In contrast, hematite-based specimens form dendritic structures in the glaze layer in an oxidizing environment and develop black.

• Journal of Korean Society of Forest Science
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• v.69 no.1
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• pp.51-55
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• 1985

Pinus thunbergii ${\times}$ P. massoniana $F_1$ hybrids were made by pollinating 15 years old Japanese black pine (P. thunbergii) with Masson pine (P. massoniana) pollen, air-shipped from Taiwan. The crossability between two species were observed. Seedling height, morphological characteristics, and the composition of phenolic compounds of these hybrids and the open-pollinated progenies of P. thunbergii were investigated. The results of the observation were as follows; 1) Number of the hybrid seeds per cone was 2.3, while open-pollinated Japanese black pine produced 26 seeds per cone. 2) All hybrid seedlings outgrew P. thunbergii, averaging 151% of mean height of Japanese black pine seedlings. The growth of hybrids was much variable, ranging from 120% to 208% of mean height of the open-pollinated progenies of P. thunbergii. 3) With regard to needle characteristics, the hybrid needles were longer and softer than those of P. thunbergii. The color of hybrid needles showed yellowish green. The positions of resin ducts in P. thunbergii observed medial, while those of hybrids showed medial and external. 4) No large difference in phenolic compound composition between hybrid and P. thunbergii was found, but phenolic compound of unknown 4 and 6 were observed only in hybrid. Further intensive work is recommended in this area.

• Korean Journal of Plant Resources
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• v.25 no.2
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• pp.285-292
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• 2012

Self-incompatibility (SI) system is a genetic barrier that prevents self-fertilization and promotes cross-pollination among different S genotypes. In many of these species, SI is controlled by a single genetic locus known as S locus, which prevents the fertilization by pollen with same locus. S RNases are the products of the S-locus expressed in the stylar tissue of Fuji Apple with gametophytic self-incompatibility system. This study investigated the various types of chemicals in order to select more effective inhibitors and activators. The effect on the inhibition of S RNase of Fuji apples was investigated $in$ $vitro$. The result showed that the enzyme activity was reduced 24.3% by Iron(II) Sulfate, significantly. $In$ $vitro$ studies of pollen growth tube showed that pollen tube growth had a higher germination rate (90%) in 10% Sucrose than in 2% sucrose extension medium. Data on the fruit set of apples treated with inhibitor and activator. Double application of $A^+$(Apple Plus, ISTECH Co. Ltd.,)+Vitamin B6 had the highest central fruit set as 86.1%(Andong). One time application of $A^{++}$Vitamin B1 in Yeongju obtained the highest central fruit set (91.9%).

• The Korean Journal of Quaternary Research
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• v.18 no.1 s.22
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• pp.31-39
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• 2004

The stable-unstable curve graph (S-U curve) and the ignition los curve graph (IL curve) for pollen composition and inorganic material content in three areas (namely, Kawadoi Basin, Moniwa-Takada moor, and Nenoshiroishi moor) were prepared to reconstruct the recurrence history of slope failure during the Holocene in Northeastern Japan. Through the S-U curve, a period with a high slope failure frequency of 5,600~4,670yrB.P. was identified in Kawadoi Basin, two of 6,390~5,070yrB.P. and 4,750~3,800yrB.P. in Moniwa-Takada moor, and three of 6,630~6,070yrB.P., 5,890~5,360yrB.P., and 5,000~4,650yrB.P. in Nenoshiroishi moor. Through the IL curve, a period with a high slope failure frequency of 5,600~5,140yrB.P. was identified in Kawadoi Basin, and two of 6,390~6,170yrB.P. and 4,750~2,840yrB.P. in Moniwa-Takada moor. Most of above-mentioned periods were included in the period of slope instability in precedent studies. However, the periods with high slope failure frequency in this study were rather different from those in precedent studies. The times and numbers associated with these periods in each study area also showed discrepancies. Accordingly, to achieve the above-mentioned purpose, more case studies must be performed to improve the reconstruction level of slope failure frequency, which could be explained through the analysis of pollen composition and inorganic material content.

• Korean Journal of Soil Science and Fertilizer
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• v.31 no.1
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• pp.9-15
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• 1998

A survey was conducted to investigate the chemical properties of soils such as pH, electrical conductivity, total organic matter content, soluble nitrate, available phosphate and major exchangeable canons, in plastic film houses at 513 sites. All the parameters surveyed in the plastic film house were much higher than those of open field soils. Particularly conspicuous was the accumulation of available P, exchangeable K and the occurrence of nitrate at relatively high concentration in both top soil(0-20 cm) and sub-soil(20-40 cm). In 70-80% of cases, the contents of available P and exchangeable K in top soils, were found to be higher than optimum levels. There was positive linear correlation between the content of exchangeable rations, and nitrate and EC of soils. The correlation coefficient was greater in the order of nitrate-EC > Mg-nitrate > K-nitrate > Ca-nitrate. The successive cultivation of horticultural crops in the plastic houses tended result in the accumulation of available P, exchangeable K and total organic matter in the soil.