• Title/Summary/Keyword: 혼합 추출물

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Spectral Mixture Analysis in forest using Landsat-7 ETM+ (Landsat-7 ETM+영상을 이용한 산림지역의 혼합화소분석)

  • 이지민;이규성
    • Proceedings of the Korean Association of Geographic Inforamtion Studies Conference
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    • 2003.04a
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    • pp.157-162
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    • 2003
  • 중저해상도 광학영상의 순간시야각(instantaneous filed of view -IFOV)에 포함되는 공간에는 반사특성이 상이한 두 개 이상의 지표물이 존재하는 경우가 대부분이다. 영상분류와 같은 기존의 영상처리기법에서는 하나의 화소가 단일의 지표물을 대표한다는 가정에서 접근하였으나, 최근 화소의 혼합정도를 세분하는 분광혼합분석(spectral mixture analysis)기법이 개발되고 있다. 분광혼합분석법을 이용하여 혼합된 화소에 포함된 지표물을 분해(unmixing) 하고 그 효과를 분석하고자 하여 경기도 광릉국립수목원의 시험림 지역을 대상으로 Landsat-7 ETM+영상을 이용하여 선형혼합 모델을 적용하였고, 그 결과 각각의 화소를 6개의 End-member의 혼합비로 구분하였다. Endmember의 비율을 나타낸 영상을 분석하여 점유비율에 따른 활엽수와 침엽수의 구분을 할 수 있었고, 각 임상별의 특징도 얻을 수 있었다. 특히 침엽수의 경우 그림자의 효과가 높다는 특성도 파악 할 수 있었다. 분광혼합분석법은 기존의 전통 분류방법과는 달리 다양한 산림의 정보를 추출해 낼 수 있다.

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Extractives from the Needles of Torreya (벼자나무 잎의 추출성분)

  • Kim, Jin-Kyu;Bae, Young-Soo
    • Journal of the Korean Wood Science and Technology
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    • v.29 no.4
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    • pp.53-59
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    • 2001
  • The needles of torreya(Torreya nucifera S. et Z) were collected, extracted with acetone-$H_2O$(7:3, v/v), fractionated with hexane, chloroform and ethylacetate, and freeze dried to give some dark brown powder. The ethylacetate soluble mixture was chromatographed on a Sephadex LH-20 column using a series of aqueous methanol and ethanol-hexane mixture as eluents. Most of the needle extractives were flavan and its methyl ether compounds such as (+)-catechin, (-)-epicatechin, (+)-gallocatechin, (-)-epigallocatechin, 3'-O-methyl-(+)-catechin and 3'-O-methyl-(-)-epicatechin including protocatechuic acid, one of benzoic acids. $^1H$- and $^{13}C$-NMR, NOE and CI-MS analyses were performed to elucidate the structures of the isolated compounds.

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Antioxidant and Tyrosinase Inhibitory Effects of the Extract Mixtures of Perilla frutescens, Houttuynia cordata and Camellia sinensis (어성초, 자소엽, 녹차 식물 추출 혼합물의 항산화 및 Tyrosinase 저해 효과에 관한 연구)

  • Lee, Kyung Eun;Lee, Eun Sun;Kang, Sang Gu
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.41 no.2
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    • pp.173-180
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    • 2015
  • In the present study, antioxidant activities and tyrosinase inhibition of Perilla frutescens, Houttuynia cordata and Camellia sinensis extracts and the extract mixtures (PHC) were investigated. PHC showed 80.2% and 98.0% of free radical scavenging activity in DPPH and ABTS analysis, respectively, and 50% tyrosinase inhibition in $1000{\mu}g/mL$ concentration. HaCaT cells did not show cell toxicity in $100{\mu}g/mL$ of the PHC. Furthermore, HaCaT cell viability by co-culture with extract H. cordata was increased more than 10% compared with untreated cells. However, the cell viability was decreased in $500{\mu}g/mL$ of the extract C. sinensis and the PHC. These results suggested that about $100{\mu}g/mL$ concentration of the PHC showed proper tyrosinase inhibitory effect and antioxidant activities. The PHC could be used as multifunctional cosmeceutical agents.

Allelopathic Influence of Alfalfa and Vetch Extracts and Residues on Soybean and Corn (알팔파와 베치의 추출물 및 잔유물의 콩과 옥수수에 대한 타감작용)

  • Ki-June Kim;III-Min Chung;Kwang-Ho Kim;Joung-Kuk Ahn
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.39 no.3
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    • pp.295-305
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    • 1994
  • Greenhouse and laboratory studies were conducted to investigate the allelopathic potential of alfalfa and vetch residues on soybean and corn using various extract concentrations (0, 5, 10, 15 and 20%, w/v) and residue rates (0, 0.25, 0.5, 0.75 and 1%, w/w). Aqueous extracts of alfalfa (Medicago sativa L.) and vetch (Vicia spp.) exhibited an allelopathic effect on soybean and corn seed germination, seedling length and weight. The degree of inhibition significantly increased as the aqueous extract concentration increased. Alfalfa and vetch 20% extracts reduced soybean seed germination, seedling length and weight by 35%, 57%, 32% and 15%, 42%, 25% respectively, when compared to control. Corn germination, seedling length and weight was inhibited by 20%, 23%, 38% by alfalfa and 19%, 18%, 35% by 20% vetch extracts. Alfalfa and vetch extracts inhibited secondary root formation and branching as the extract concentration increased. Alfalfa and vetch 20% extracts inhibited by 41% and 32% secondary root numbers, respectively as compared to control. It was found that the aqueous extract of alfalfa resulted in greater reduction in germination, seedling length and weight of soybean than that of vetch. Alfalfa and vetch 1% residue rate inhibited soybean plant height by 30% and 10%, leaf area by 31% and 23%, and dry weight by 18% and 1%, nodule number by 27% and 20% also. Alfalfa and vetch residue significantly enhanced plant height, leaf area and dry weight of corn. The maximum stimulation occurred with 0.25% and 1% of alfalfa and vetch residue rates, respectively. Plant height, leaf area, and dry weight increased by 23%, 59%, 58% and 17%, 52%, 94% with alfalfa and vetch residues of 0.25% and 1%, respectively. This study demonstrates that there is an allelopathic potential resulting from alfalfa and vetch residues on soybean growth and yield. It also suggests that these residues may affect crop growth and development due to the inhibitory or stimulatory effects of allelochemicals existing in the residue.

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Concentration of Polyunsaturated Fatty Acids from Anchovy Oil by Supercritical Carbon Dioxide (초임계 이산화탄소에 의한 멸치어유의 고도불포화지방산 농축)

  • Lim, Sang-Bin;Jwa, Mi-Kyung;Song, Dae-Jin
    • Korean Journal of Food Science and Technology
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    • v.30 no.4
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    • pp.848-854
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    • 1998
  • To concentrate polyunsaturated fatty acids from anchovy oil by supercritical carbon dioxide $(SC-CO_2)$, effects of different adsorbents, cosolvents and extraction pressures on the extraction yield and fatty acid composition in the extract and the residue fractions were investigated. Anchovy oil mixed with silver nitrate-coated silica gel showed higher extraction yield and concentration of EPA and DHA in the residual fraction than that mixed with only silica gel at $60^{\circ}C/345{\;}bar$. Ethyl acetate was a promising cosolvent for concentrating polyunsaturated fatty acids in the residual fraction from anchovy oil mixed with silica gel. For the extraction pressures tested, 276 bar showed a maximum value in the extraction yield and concentration of EPA and DHA in the residue. Starting with anchovy oil containing 13.3% EPA and 16.2% DHA mixed with a silver nitrate-coated silica gel, the residue fraction containing 28.2% EPA and 38.3% DHA was obtained when ethyl acetate was used as a cosolvent with $SC-CO_2$ at $60^{\circ}C/276{\;}bar$.

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Method for Supplementing Lecithin to Ginseng Extract (레시틴이 강화된 인삼 추출물 제조 방법)

  • Park, Soon-Hye;Kim, Il-Woong;Kim, Dong-Man;Kim, Si-Kwan
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.35 no.9
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    • pp.1245-1250
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    • 2006
  • This study was carried out to develop the method of preparing lecithin-fortified ginseng extract. Firstly, soybean lecithin was mixed with soybean oil (LCS) in varying ratio (2.5%, 5%, 10% and 20%). Then, one part volume of LCS was mixed with three parts volume of ginseng extract with 10% solid matter content and the mixture was vortexed vigorously. Finally, the mixture was spinned at the speed of 3,000 rpm for 30 minutes to separate oil and aqueous ginseng extract layer (AG). AG was then subjected to qualitative and quantitative analysis of phospholipids and ginsenosides. Fatty acid composition and crude fat content before and after LCS was determined. Stability of lecithin in ginseng extract was determined by analyzing phospholipid content in the one third upper and lower layer of the concentrated AG in Falcon tubes while storing the LCS treated concentrated AG in 4, 25 and 40oC for 6 months. Ratio of lecithin transferred to AG increased with the increase in lecithin content of soybean oil. There was no significant change in fatty acid composition and crude fat content, and ginsenoside content in the ginseng extract before and after LCS treatment. TLC and HPLC pattern of saponin fraction before and after treating the ginseng extract with LCS demonstrated no observable difference. There was no change in lecithin content in the upper and lower one third layer of ginseng extract in the tubes after storing the concentrated AG in 4, 25 and $40^{\circ}C$ for 6 months. Ginsenosides HPLC pattern was not changed when stored the LCS-treated ginseng extract in those conditions for six months, indicating satisfiable stability of the LCS-treated concentrated ginseng extract. From these results, it can be concluded that treatment of the ginseng extract with lecithin containing soybean oil is a labor effective method with satisfiable stability to fortify lecithins to ginseng extract.

Effect of a Combined Extract of Orostachys japonicus with Medicinal Plants on the Lipid Composition of the Liver and Kidney from Streptozotocin-induced Diabetic Rats (와송과 생약재 복합물이 Streptozotocin 유발 당뇨쥐의 간장 및 신장 조직의 지질성분에 미치는 영향)

  • Shin, Jung-Hye;Lee, Soo-Jung;Seo, Jong-Kwon;Lee, Hyun-Ju;Ju, Jong-Chan;Sung, Nak-Ju
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.41 no.4
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    • pp.510-518
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    • 2012
  • This study examined the biological activity and synergistic effects of an extract of Wa-song ($Orostachys$ $japonicus$, OE), a medicinal plant mixture (MPE) and a combination of both at different ratios (1:1, OMPE-1 and 3:1, OMPE-3). Extracts of the medicinal plants mixture were comprised of Baekbokyung, Changchul and Sa-in at the same ratio. The antioxidant activity of the extracts and their complex were tested $in$ $vitro$. The $in$ $vivo$ antioxidant activity was also analyzed by examining the lipid composition in the liver and kidney of streptozotocin (STZ)-induced diabetes rats. The nitric oxide radical scavenging activity was more than 50% in OMPE-3 at a 1,000 ${\mu}g$/mL concentration. Regarding metal ions, such as $Fe^{2+}$ and $Cu^{2+}$, the antioxidant activity of OMPE-1 and OMPE-3 was higher than that of OE and MPE. OMPE-1 and 3 had higher activity on $Cu^{2+}$ ions than $Fe^{2+}$ ions. The ${\alpha}$-glucosidase inhibition activity of the OE extract was higher than that of MPE and OMPE-1 but the relative activity of OMPE-3 was significantly higher than the others. Freeze-dried MPE, OMPE-1 and OMPE-3 were added to the diet at a level of 1% given to STZ induced diabetes rats for 4 weeks. The OMPE-1 and OMPE-3 administered groups showed significant decreases in the total lipid, total cholesterol and triglyceride levels in the liver and kidney. In these groups, the glycogen accumulation level of the liver was increased significantly. The content of thiobarbituric acid reactive substances (TBARS) in the liver and kidney was decreased but the DPPH radical scavenging activity was increased. These results suggest that Wa-song extract exhibits antioxidant and antidiabetic activity, which are enhanced by a complex with a medical plants extract.

Antimicrobial Effect of Lonicerae Flos Extracts on Food-borne Pathogens (식중독 유발세균의 증식에 미치는 금은화 추출물의 항균효과)

  • Bae, Ji-Hyun;Kim, Mi-Soon;Kang, Eun-Hae
    • Korean Journal of Food Science and Technology
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    • v.37 no.4
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    • pp.642-647
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    • 2005
  • Lonicerae Flos was extracted with methanol and successively fractionated with petroleum ether, chloroform, ethyl acetate, and methanol to investigate their antimicrobial effects against food-borne pathogens and food spoilage bacteria using paper disc method. Ethyl acetate extracts of L. Flos showed highest antimicrobial activity against Shigella dysenteriae. Synergistic effect in inhibition was observed when L. Flos extract was mixed with Artemisa capillaris extract as compared to using each extract alone. Growth inhibition curves were determined using ethyl acetate extracts of L. Flos against Staphylococcus epidermidis and S. dysenteriae. Ethyl acetate extract of L. Flos had antimicrobial activity against S. dysenteriae at 3,000 ppm, retarding growth of S. dysenteriae up to 12 hr.

Antioxidant Activity of Various Solvent Extracts from Freeze Dried Kimchi (김치 용매 추출물의 항산화성)

  • 이영옥;최홍식
    • Journal of Life Science
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    • v.6 no.1
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    • pp.66-71
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    • 1996
  • Antioxidative activity of various solvent extracts from freeze dried Kimchi was studied during the oxidation process of linoleic acid mixture system. The aqueous model systems were used for the evaluation of antioxidative activity of solvent extracts during the oxidative reaction at 37$^{\circ}C$ by the determination of peroxide value. The linoleic acid mixture for the aqueous model systems was consisted of linoleic acid (64.6%), oleic acid(27.4%), and other acids in ethanolic phosphate buffer (pH 7.0). Water and methanol extracts of Kimchi had a considerable antioxidative activity with the inhibition of peroxides formation during the autoxidation of linoleic acid mixture in aqueous model systems. Antioxidative activity of 75% methanol extract of 7 day-fermented-Kimchi was relatively higher than that of others, however lower than alpa-tocopherol and butylated hydroxyanisol. The antioxidative effect of 75% methanol extract of 7 day-fermented-Kimchi was increased by the its concentration from 0.5% to 2.5% in the oxidation reactions of aqueous model systems.

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