• The Korean Journal of Applied Statistics
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• v.22 no.4
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• pp.709-721
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• 2009

This paper discusses an efficient image segmentation using morphological watershed algorithm that is robust to noise. Morphological image segmentation consists of four steps: image simplification, computation of gradient image and watershed algorithm and region merging. Conventional watershed segmentation exhibits a serious weakness for over-segmentation of images. In this paper we present a morphological edge detection methods for detecting edges under noisy condition and apply our watershed algorithm to the resulting gradient images and merge regions using Kolmogorov-Smirnov test for eliminating irrelevant regions in the resulting segmented images. Experimental results are analyzed in both qualitative analysis through visual inspection and quantitative analysis with percentage error as well as computational time needed to segment images. The proposed algorithm can efficiently improve segmentation accuracy and significantly reduce the speed of computational time.

• Journal of KIISE:Software and Applications
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• v.32 no.6
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• pp.523-531
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• 2005

Existing methods for color image segmentation using diffusion can't preserve contour information, or noises with high gradients become more salient as the number of times of the diffusion increases, resulting in over-segmentation when applied to watershed. This paper proposes a method for color image segmentation by applying morphological operations together with nonlinear diffusion For an input image, transformed into LUV color space, closing by reconstruction and nonlinear diffusion are applied to obtain a simplified image which preserves contour information with noises removed. With gradients computed from this simplified image, watershed algorithm is applied. Experiments show that color images are segmented very effectively without over-segmentation.

• The KIPS Transactions:PartB
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• v.16B no.2
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• pp.131-140
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• 2009

This paper presents a new algorithm to the segmentation of the FISH images. First, for segmentation of the cell nuclei from background, a threshold is estimated by using the gaussian mixture model and maximizing the likelihood function of gray value of cell images. After nuclei segmentation, overlapped nuclei and isolated nuclei need to be classified for exact nuclei analysis. For nuclei classification, this paper extracted the morphological features of the nuclei such as compactness, smoothness and moments from training data. Three probability density functions are generated from these features and they are applied to the proposed Bayesian networks as evidences. After nuclei classification, segmenting of overlapped nuclei into isolated nuclei is necessary. This paper first performs intensity gradient transform and watershed algorithm to segment overlapped nuclei. Then proposed stepwise merging strategy is applied to merge several fragments in major nucleus. The experimental results using FISH images show that our system can indeed improve segmentation performance compared to previous researches, since we performed nuclei classification before separating overlapped nuclei.