• Journal of Life Science
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• v.32 no.1
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• pp.11-22
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• 2022

N-methyl-D-aspartate (NMDA) receptors have received considerable attention regarding their involvement in glutamate-induced neuronal excitotoxicity. Resveratrol has been shown to exhibit neuroprotective effects against this kind of overactivation, but the underlying cellular mechanisms are not yet clearly understood. In this study, HT-22 neuronal cells were treated with NMDA in Mg²⁺-free buffer and subsequently used as an experimental model of glutamate excitotoxicity to elucidate the mechanisms of resveratrol-induced neuroprotection. We found that NMDA treatment causes a drop in MTT reduction ability, disrupts inside-negative transmembrane potential of mitochondria, depletes cellular ATP levels, and stimulates intracellular ROS production. Double fluorescence imaging studies demonstrated an increased formation of mitochondrial permeability transition (MPT) pores accompanied by apoptotic cell death, while cobalt protoporphyrin and bilirubin showed protective effects against NMDA-induced mitochondrial injury. On the other hand, zinc protoporphyrin IX significantly attenuated the protective effects of resveratrol which was itself shown to enhance heme oxygenase-1 (HO-1) mRNA and protein expression levels. In cells transfected with HO-1 small interfering RNA, resveratrol failed to suppress the NMDA-induced effects on MTT reduction ability and MPT pore formation. The present study suggests that resveratrol may prevent mitochondrial injury in NMDA- treated HT-22 cells and that enhanced expression of HO-1 is involved in the underlying cellular mechanism.

• Journal of Life Science
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• v.31 no.12
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• pp.1088-1093
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• 2021

Jeju horse (Equus ferus caballus) is a Korean horse breed that has been native to Jeju Island for a long time. Jeju horses are used as racehorses, and their racing ability is a major economic trait. The role of the myostatin (MSTN) gene in skeletal muscle mass has been studied in various mammals, and mutations in the MSTN gene are known to affect the racing ability and stamina of thoroughbreds. In this study, we compared the frequency of mutations in the MSTN gene in several horse breeds, including 1,433 Jeju horses. Among the mutations (ECA18 g.66493737C>T) in the MSTN gene, the long-distance aptitude genotype (TT) was found to have a frequency of 0.826 in Jeju horses, which was higher than that in Halla horses (0.285) and thoroughbreds (0.252). The genotypes and arrival records of Jeju horses were compared according to various distances (400 m, 800 m, 900 m, 1,000 m, 1,110 m, and 1,200 m). According to the results, the CT type showed a faster-reaching record than the TT type in races of less than 1,000 m. However, almost identical results were confirmed in races over 1,110 m. This study suggests that the MSTN mutation in Jeju horses may be related to race distance aptitude. In future research, the data in this study can be used for developing markers related to race distance aptitude and racing abilities in Jeju horses.

• Ecology and Resilient Infrastructure
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• v.9 no.3
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• pp.183-193
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• 2022

The growth inhibitory effect of Microcystis aeruginosa according to the ultrasonic irradiation time was evaluated using a large algae sample volume (10 L) for various ultrasonic irradiation times (0.5, 1, 1.5, 2, 2.5 and 3 hr) at a laboratory scale. Based on the analysis of Chl-a and cell number of M. aerginosa, algae growth inhibition was observed with the decrease in Chl-a and cell number in all experimental groups after the ultrasonic irradiation. For the experimental group (T_B, T_C, T_D) with an ultrasonic irradiation time of less than 2 hours, rapid regrowth of algae was observed after growth inhibition, but the experimental group (T_E, T_F, T_G) with an irradiation time of more than 2 hours successfully inhibited algal growth lasting one or two more days. Based on the comparison of the recovery time to initial cell number the experimental group (T_B, T_C, T_D) took less than 20 days whereas the experimental group (T_E, T_F, T_G) took about 30 days. Correspondingly, the experimental group showed a high first order decay rate (𝜅) in proportion to the ultrasonic irradiation time during the growth inhibition period. Additionally, the specific growth rates (𝜇) during regrowth in the experimental group with irradiation time of more than 2 hours were relatively low compared to those in the experimental group with less than 2 hours. Therefore, ultrasonic irradiation for more than 2 hours is required for long-term (30 days) inhibition of algal growth in stagnant waters. However, the appropriate ultrasonic irradiation time for algae growth inhibition should be determined according to various field conditions such as the volume of stagnant water, water depth, flow rate, algae concentration, etc. Finally, damages to the algal cell surface and cell membrane were clearly observed, and both destruction and disturbance of gas vesicles of M. aeruginosa in the experimental group were discovered, indicating the growth inhibitory effect of Microcystis aeruginosa according to the ultrasonic irradiation time was confirmed.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.30 no.3
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• pp.252-258
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• 1985

An experiment was conducted at the Yeongnam Crop Experiment Station to obtain basic informations about cultural techniques for high yielding by manipulating plant spacing using two rice cultivars, Samgangbyeo (Indica/Japonica type) and Nakdongbyeo (Japonica type), and four plant spacings, 10${\times}$10cm, 20${\times}$20cm 30${\times}$30cm and 40${\times}$40cm, with 4 kinds of seedling number per hill, 1,3,5 and 7, respectively. High photosynthetic efficiency (Eu) exhibited at the Samgangbyeo compared to Nakdongbyeo regardless of plant spacings and seedling numbers. For Samgangbyeo, Eu value was the highest at the 20${\times}$20cm plant spacing and five seedlings and seven seedlings per hill showed high Eu values at 10${\times}$10cm plant spacing and 20${\times}$20cm plant spacing, respectively, while other plant spacings were not significantly differed among seedling numbers. For Nakdongbyeo, however, one seedling plot obtained high Eu value at the 10${\times}$10cm plant spacing while this Eu value increased as the seedling number per hill increased in other plant spacings. There was a high positive correlation between rice grain yield and total competition index for both cultivars while kind of relationships differed in these two cultivars; linear relationship for Samgangbyeo and exponential relationship for Nakdongbyeo, respectively. Competition index between rice hill was more significant than within rice hill for Samgangbyeo while both competition indexs were important for Nakdongbyeo to increase rice yield.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.38 no.6
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• pp.499-506
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• 1993

The contents of sulfur, sulfur-containing protein and amino acids of soybean seeds of 518 genotypes as well as their inheritance and selection efficiency in early breeding generation were measured to facilitate breeding for soybean with high sulfur-containing amino acids. Average seed sulfur content of 518 cultivars was 0.33%, and ranged from 0.20 to 0.45%, and that of 30 wild soybeans was 0.35%, and ranged form 0.19 to 0.62%. Correlation coefficients between seed sulfur content and sulfur-containing protein and amino acids were 0.924$^{**}$ and 0.974$^{**}$, respectively. Seed sulfur content was tended to be high in soybean genotypes with late maturity, seed coat bloom, or green cotyledon. Sulfur content had -0.312$^{**}$ correlation coeficient with sugar content and -0.384$^{**}$ with 100 seed weight. Seed sulfur content was inherited quantitatively, in which additive effect was greater than dominant one, and proportion of genes with positive effects was similar to those with negative ones. Estimated narrow- and broad-sense heritabilities were 0.75 and 0.88 for seed sulfur content, respectively. Heritability measured from selection in early breeding lines for high or low seed sulfur content was 60～62.5% or 50～62,5%, respectively. And selection for high sulfur content increased by 14.7～18.8%, whereas that for low one decreased by 8.8～15.6%, when compared to that of random population. Therefore selection in early generation seemed to be clearly effective.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.32 no.2
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• pp.224-233
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• 1987

Most of the japonica rice varieties preferred for high eating quality are liable to lodging even under moderate rate of nitrogen application. This lodging liability has been a critical limit even for proper evaluation of physio-logical characteristics of those varieties exhibitable under higher nitrogen levels. Use of recent inhibitors of gibberellin biosynthesis such as ‘Pac1obutrazol’ may allow us to overcome this barrier. The responses of four high eating quality varieties to nitrogen application to the level of 150kg N per ha were evaluated with and without use of Paclobutrazol in comparison with a non-lodging, improved short japonica, Dongjin and a non-lodging, high yielding indica x japonica Milyang 23. The four were Damageum (the best eating quality in the 1930s), Nongrim 6 (the best in the 1960s), Chuchung (the best since 1970s), Koshihikari (the best in Japan since 1960s). As expected increased application of nitrogen increased plant height, length of the 3rd internode, and lodg-ing liability, being measured as culm breaking load, in all varieties tested and caused actual lodging in the fiel from the 50kg Nfha level in Damageum and Koshihikari and at the level of l50kg Nfha in Nongrim 6. Applica-tion of Pac1obutrazol (0.6%G) 15 days before heading reduced plant height, length of the 3rd internode and lodging liability being measured as culm breaking load in all varieties tested. However, the application of Pac1obutrazol during active tillering stage resulted in decreased culm breaking load in Damageum, Nongrim 6, and Koshihikari in spite of the decreased plant height and culm length as in the other varieties. Maximum yield was obtained with 100kg Nand 30kg Pac1obutrazol at 15 days before heading in Nongrim 6, 150kg N and 30kg Pac1obutrazol at 15 days before heading in Damageum, and 150kg N and 20kg Paclobutrazol at 20 days after transplanting plus 30kg Paclobutrazol at 15 days before heading in the variety Koshinhikari and Chuchung. Under a sensory evaluation of cooked rice, the four high eating quality varieties were not different in rank and Paclobutrazol treated rice was not distinguished from the untreated in eating quality.

• Korean journal of applied entomology
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• v.61 no.3
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• pp.399-408
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• 2022

There has been much debate on the morphometric divergence between the recently identified Apis cerana koreana and Apis cerana honey bees. The aim of this study was to obtain phenotypic information that can be used to compare A. c. koreana data with other A. cerana subspecies data from open resources and determine breeding results on the basis of morphometric traits. To differentiate A. c. koreana, we investigated 22 classic morphological characteristics; royal jelly secretion; and the weight of workers, queens, and drones of A. c. koreana bred in Korea. To define the selection results, we used the geometric morphometric method. The artificially selected A. c. koreana secreted significantly more royal jelly (1.18 times) than the naturally selected A. c. koreana, which positively influenced the health of the colonies. These honey bees were identified more clearly with the geometric morphometric method than with the classic morphometric method, which is traditionally used to determine the subspecies. Large trends were noted for A. c. koreana on the basis of our results and literature from the 1980s regarding A. cerana sizes in Korea (tarsal index, length of forewing, and cubital index were measured). The cluster analysis revealed the proximity of A. c. koreana, A. cerana in China, and A. c. indica on the basis of eight classic characters, which, perhaps, relay the origin of the honey bees. The results of this study defined the morphometric responses of A. c. koreana honey bees to geographic isolation, climate change, and selection, which are important to identify, protect, and preserve honey bee stock in Korea.

• Journal of Mushroom
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• v.21 no.3
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• pp.132-139
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• 2023

Light plays an important role in fruit-body development and morphology during Pleurotus spp. cultivation. To understand the effects of light color on fruit-body properties, we evaluated the fruit-body characteristics of Pleurotus spp. Varieties cultivated under blue, red, and purple LED light sources. The main results are as follows: The overall fruit-body shape showed differences depending on the color of the LED light. The fruit-bodies of mushroom cultivated under blue and purple light were generally similar to the mushroom shapes typically produced, while those of mushroom cultivated under green light were abnormally shaped, probably due to the absence of effective light source. The average cap lightness of mushrooms cultivated under blue, green, and purple LED lights was 57.0, 57.4, and 59.4, respectively. The average cap lightness of all varieties except Wonhyeong1ho and Hwang-geumsantari cultivated under the three LED light sources were statistically significantly different (P<0.05). The cap redness varied significantly depending on the LED lighting and variety. Only Gonji7hoM, the cap color mutant of Gonji7ho, showed negative cap redness values under all three LED light sources. Among the eight varieties excluding Gonji7ho, the highest cap redness was observed when cultivated under the blue LED. The average harvest weight of the varieties cultivated under purple, blue, and green LED light were 68.0, 58.3, and 50.1 g, respectively. The yield of Gonji7ho, the mushroom variety with the highest yield, cultivated under blue, green, and purple LED light were 92.8, 77.1, and 98.6 g, respectively. The earliness when grown under the purple, blue, and green LED lights were 5.3, 5.8, and 5.8 days, respectively. Among the varieties, six, three, and two cultivars showed the shortest earliness under the purple, green, and blue LED, respectively. The fruit-body lengths were 66.4, 51.8, and 46.8 mm when cultivated under green, purple, and blue lights, respectively. These results are expected to serve as a foundation for producing mushrooms with traits demanded in the market.

• Journal of agriculture & life science
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• v.50 no.5
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• pp.173-182
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• 2016

This study was aimed to identify the phenotypic relationships between calving interval and lactation curve parameters in Korean Holstein cow. The data of 36,505 lactation records was obtained from the Dairy Herd Improvement program run by Dairy Cattle Improvemnet Center of National Agricultural Federation of Korea. All lactation records were collectied from the multiparous cows calving between 2011 to 2013. The estimated lactation curves were drawn using Wood model based on actual milk yield records, and NLIN Procedure of SAS program (ver. 9.2). General linear multivariate models for calving interval, 305-d milk yield, lactation parameters(A, b, c), persistency, peak day, and peak yield included fixed effects of calving year-season (spring, summer, fall and winter) and parity(2, 3 and 4). For calving interval, 305-d milk yield, lactation parameters(A, b, c), persistency, peak day and peak yield, all two fixed effect(calving year-season, parity) were significant(p<0.05). The estimated lactation functions using Wood model for 2, 3, and 4 parity were yt=24.66t0.175e-0.00302t, yt=24.69t0.192e-0.00334t, and yt=24.22t0.200e-0.00341t, respectively. Phenotypic correlation (partial residual correlation) between calving interval and 305-d milk yield, A, b, c, persistency, peak day, and peak yield were 0.093, -0.014, 0.028, -0.046, 0.099, 0.085, and 0.052, respectively. To conclude, if calving interval increase then ascent to peak, persistency, peak day and peak yield are increase, and descent after peak is decrease. So, total 305-d milk yield is increase.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.41 no.1
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• pp.68-76
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• 1996

To elucidate the optimum fertilizer level and application method for band application under puddled-soil drill seeding in Jeonbuk series of fluvio-marine alluvial soil at National Honam Agricultural Experiment Station in 1995, using Dongjinbyeo, slow releasing compound fertilizer of 100％ and 80％ to conventional application level was applied totally as basal fertilizer simultaneously with seeding under 3cm and 5cm depth from soil surface in a distance of 4cm from the seeded row. Plant height was taller and tiller number was higher in band application than conventional application but ratio of effective tiller was vice versa. Panicle number was more but ratio of effective tiller ratio was lower in 100％ than 80％ level of band application and they were higher in 3cm than 5cm depth from soil surface. Leaf area index and dry weight was higher in conventional application at early growth stage but was vice versa after maximum tillering stage, and they were higher in 3cm depth at early growth stage but 5cm depth after maximum tillering stage. NH$_4$-N in soil was higher in conventional application at 25 days after seeding but, thereafter was lower than band application and it was higher in 3cm than 5cm depth till 40 days after seeding but was versa, thereafter. Lodging degree was slightly higher in band application, 100％ level and 5cm depth than in their counterparts. Panicle number and grain number per $m^2$ was lower in conventional application than 80％ or 100％ level of band application without significant difference between band application levels or application methods. Yield was higher at 80％ level of band application under 3cm depth than conventional application, but no significantly different among other application methods. Therefore, 80％ level of band application under 3cm depth of soil surface was more effective for puddled-soil drill seeding on the basis of the reduction of application efforts, better plant growth and higher yield in rice.