• Journal of Korean Ophthalmic Optics Society
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• v.13 no.2
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• pp.17-22
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• 2008

To develop an imaging optical system for the 545 nm fluorescent plate of X-ray. Methods: We designed and manufactured a new imaging optical system for the 545 nm fluorescent plate of X-ray by Sigma 2000 program after deciding the design comparison standards referred to Canon CX2-70 model. Results: The characteristics of the new imaging optical system for the 545 nm fluorescent plate of X-ray have the magnification of -0.225x, the image field size of $90mm{\times}90mm$, and the 0.033 mm resolution line width at the 30% MTF value criterion. These mean that the new model has a capability of deciphering for the more large screen and the resolution of deciphering is superior to that of Canon CX2-70 model. Also the image side NA (-0.196) of the new model is about $\sqrt{2}$ times than that (-0.139) of CX2-70 and the object side NA (0.044) of the new model is about 2 times than that (0.022) of CX2-70. These mean that the sensitivity of the film in the new design model is able to be increased to about 4 times and there is the possibility of reducing the bombed time of X-ray to 1/4 times. Conclusions: We could design and manufacture the imaging optical system for the 545 nm fluorescent plate of X-ray having the possibility of reducing the bombed time of X-ray to 1/4 times in comparision to Canon CX2-70 model, the characteristics of which have the image field size of $90mm{\times}90mm$ and the MTF of 30% or more at 15 lines/mm criterion.

• Proceedings of the Korea Water Resources Association Conference
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• 2012.05a
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• pp.674-677
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• 2012

본 연구에서는 기존 매립이나 지반보강재로 사용되던 소각재 용융슬래그를 고부가가치 수처리 여재로 활용하기 위하여 형광성 박테리아를 통한 Microtox 생물검정법으로 독성을 평가하였다. 소각재 용융슬래그의 독성평가 대조군으로는 기존의 수처리 여재인 입상활성탄(석탄계/야자계/목탄계), PP PE펠렛(Poly-Propylene Poly-Ethylene pallet), 표준여과사를 비교하였으며, 시료의 용출시험은 US EPA에서 제안한 TCLP 방법을 사용하였다. Microtox Acute Toxicity 평가 결과, 독성순위는 석탄계 입상 활성탄, 소각재 용융슬래그, 목탄계 입상활성탄, 야자계 입상활성탄, 표준여과사, PP PE 순으로 나타났으며, 실험에 사용된 모든 수처리 여재들은 급성독성을 고려하지 않는 무독성으로 나타났다. 따라서 소각재 용융슬래그가 수처리를 목적으로 수체에 편입시켜도 기존의 수처리 여재들과 비교할 때 수환경에 미치는 영향은 미미할 것으로 판단된다.

• The Journal of Korean Society for Radiation Therapy
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• v.20 no.1
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• pp.31-36
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• 2008

Purpose: X ray irradiates material for dose distribution confirmation through material color variation to evaluate about possibility. Materials and Methods: That is rare earth material to pure KCl and KCl impurity Eu adding 0.5mol% by Czochralski method each single crystal grow and observed color variation of KCl X ray irradiation use of linear accelerator. Results: High energy X ray irradiation KCl:Eu show the blue fluorescence with purple color that pure KCl single crystal can confirm by show was not observed, but was colored violet. Conclusion: Colors variation of KCl founds stable color center from radiation and this color variation will be used usefully to X ray measurement material and phantom.

• Microbiology and Biotechnology Letters
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• v.30 no.3
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• pp.235-240
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• 2002

Streptomycetes is a group of Gram-positive soil bacteria that growas a branching vegetative mycelium leading to the formation of spores, and display a physiological differenti-ation related to the synthesis of many secondary metabolites including antibiotics. Their complex life cycle and multicellular differentiation require various levels of regulation and types of signal transduction systems including eukaryotic-type serine/threonine protein kinases and prokaryotic-type histidine/aspartic acid protein kinases. Akt kinase that was found in cells is a sorine/threonine kinase controlling signal pathway for multi-tude of important cellular events. The activation or inactivation of Akt kinase in the cell is one of the critical regulatory points to deliver cell proliferation, differentiation, survival or apoptosis signal. To find the regula-tory protein homologous to Akt in Streptomyces, the fluorescien-labeled synthetic peptide (FITC-TRRSR-TESIT) was designed from the consensus sequence of target proteins for Akt kinase. From the difference of the mobility between the nonphosphorylated and phosphorylated synthetic peptides on Agarose gel electro-phoresis, the Akt-phosphorylating activity was monitored. The cell-free extract prepared from Streptomyces griseus IFO 13350 and the Akt homologous protein was purified by ammonium sulfate fractionation and many steps of column chromatographies such as, DEAE-Sepharose, Mono Q, Resource Phenyl-Soporose and Gel permeation column chromatographies. As a result, the protein phosphorylating the fluorescien-labeled Akt substrate was identified and it's molecular weight was estimated as 39 kDa on SDS-PAGE.

• Development and Reproduction
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• v.2 no.1
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• pp.1-8
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• 1998

In mammalian ovary, major portion(>99%) of ovarian follicles undergo atresia. Recent studies have shown that this phenomenon is mediated via GC apoptosis. Ceramide, a product of sphingomyelin hydrolysis, has been proposed as a novel lipid second messenger with specific roles in mediating antiproliferative responses including apoptosis and cell cycle arrest. In the present study, we have examined the effect of ceramide on apoptotic cell death of GC in vitro. GCs were harvested by squeezing the antral follicles from the immature mice (3-4 weeks) and cultured in MEM medium with 10% fetal bovine serum. The cells were treated with various concentrations of ceramide (0 to 50 \mu M)and cultured up to 24 h.Cell death was determined by MTT cell viability assay and apoptosis was examined by acridine orange staining, in situ 3'-end labeling(TUNEL), and flow cytometry. Ceramid treatment induced apoptotic cell death of GC in a time- and a dose-dependent manner. Results of flow cytometric analysis showed that creamide-induced cell death was mostly confined to the $G_{0}$/$G_{1}$ cells. these results provide an evidence for ceramide as a lipid second messenger of apoptosis in mouse GC.

• Korean Journal of Microbiology
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• v.52 no.3
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• pp.249-253
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• 2016

The inky cap, Coprinellus congregatus, produces several laccase isozymes during its life cycle: both hyphal tip laccase and sclerotial laccase are involved in the fungal development. When this fungus was transferred to an acid liquid medium (pH 4.0-4.5), a new laccase was synthesized and secreted into the culture supernatant. In order to examine its regulation by external pH, green fluorescent protein gene was ligated at the downstream of the promoters having different lengths. These expression vectors having different promoter lengths were inserted into the fungal transformation vector, pBARGEM7-1. These expression vectors were introduced to the mating type a1 and a2 monokaryons, and the transformants were selected by the phosphinothricin resistance. Transformant a1 (a1TF) and transformant a2 (a2TF) were mated with each other to generate homozygotic dikaryon transformants. All these transformants were grown in neutral liquid medium for 5 days, and then the whole cell homogenates were transferred to the acidic liquid medium (pH 4.1). After 36 h incubation at $25^{\circ}C$, cells were harvested for the analysis of GFP expression. GFP expression was detected in the transformant having full-length promoter (2.0 kb), but other transformants having shorter length promoter (shorter than 1.29 kb) failed to show the fluorescence. Therefore, the acid-responsive element in the laccase promoter should be localized between -2.0 kb ~ -1.29 kb region.

• The korean journal of orthodontics
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• v.28 no.6 s.71
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• pp.915-926
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• 1998

The cytoskeleton has been shown to form a network, connecting the extracelluar matrix via integrin with the nucleus and the cytoplasmic constituents of the cell. It is therefore assumed that the cytoskeleton may mediate signals generated by perturbations originating in the matrix. The purpose of this study is to examine the effect of cytoskeletal change on osteoblastic cell activities. The author cultured osteoblastic cells obtained from neonatal mouse calvaria. The cells were teated with cytochalasin B(CB) or colchicine (COL) at four concentrations for 3 hours and after another 24 hours the conditioned media was collected and assayed for prostaglandin $E_2\;(PGE_2)$, interleukin-6(IL-6), tumor necrosis factor-$\alpha$ (TNF-$\alpha$) and matrix metalloproteinase-1(MMP-1). In addition, the cytoskeletal protein actin were observed by immuno-fluorescence. The results were as follows: 1. The production of $PGE_2$ showed the tendency to be increased in CB-treated group. $PGE_2$ was increased in COL-treated group dose-dependantly, 2. IL-6 production, in CB-treated group, was increased, except at 1.0 ${\mu}g/ml$. IL-6 was induced in COL-treated group. 3. TNF-$\alpha$ production was increased in CB-treated group, except at 1.0 ${\mu}g/ml$, and in COL-treated group, that was increased. 4. The MMP-1 production was decreased in CB-treated soup and was not changed in COL-treated group, which could be selectively visualized by immunoblotting with monospecific antibody. 5. The cytoskeletal actin stress fibers were disappeared and the cells showed to be rounded in CB-treated group. These results indicated that there are a relationship between the cytoskeletal rearrangements and osteoblastic cell activities, especially in release of paracrine/autocrine factors, such as $PGE_2$, IL-6, and TNF-$\alpha$.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2008.05a
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• pp.705-708
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• 2008

ZnS는 전기적 에너지를 받으면 전자와 정공이 무수히 발생하며 이들이 평형상태로 갈 때 보다 높은 준위로 여기 되면 빛이 생성 될 수 있다. 전계발광(EL) 표시장치를 제작하기 위하여 일본의 High purity 사의 제품이 비교적 Electron Beam으로 증착하기에 우수하였고, 휘도도 상용화된 800fL에 80% 수준으로 제작과정을 개선하여 더욱 우수한 결과를 얻을 수 있고 본 연구는 Yellow의 경우 650fL의 휘도를 얻었고, Green의 경우 350 fL의 휘도를 얻었다. Electron Beam 제작용으로 자체 제작된 기판 홀더로 막을 제작한 결과 두께 균일도는 6% 내외의 결과로 상당히 우수한 특성을 나타내었다.

• Proceedings of the Korean Nuclear Society Conference
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• 1995.05b
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• pp.875-881
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• 1995

원자력 연구소는 국제원자력기구(IAEA/RCA) 주관하에 1990년부터 1993년 사이 3차에 걸쳐 실시한 개인선량계에 대한 국제상호비교에 참여하였다. 국제 상호비교에 참여하여 사용된 개인선량계는 방사선작업종사자에 대한 외부방사선으로부터 피폭관리를 위하여 기 사용중인 Taedyne Isotope 사의 PB-3타입의 열형광선량계이며 선량계판독용으로 Tdedyne 9150자동판독기를 사용하였다. 본 논문에는 3차에 걸쳐 국제상호비교결과를 요약하였으며 저 에너지의 엑스선에 경우를 제외하고 모든 조사방사선에 대해 0.78에서 1.07사이에 상대비율로 결과를 나타내었다. 또한 미국기준 ANSI Nl 3.11에 의해 성능을 시험한 결과 모든 조사방사선장에 대해서 허용기준 0.5이하를 나타내었다.

• Proceedings of the KOSOMBE Conference
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• v.1998 no.11
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• pp.66-69
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• 1998

Comparative genomic hybridization (CGH) is an important molecular cytogenetics technique that maps abnormal copy number of specific DNA sequence of the chromosome. CGH is based on quantitative digital image analysis of ratio images from fluorescently labeled chromosomes. In this paper, we would like to introduce how recently developed image analysis algorithms are used for CGH techniques. To average the ratio profile of each chromosome, binarization, skeletonization, and stretching of chromosome images have been studied. Developed algorithms have been implemented in the karyotyping system ChIPS commercially developed at Biomedlab Co. Ltd.