• Title/Summary/Keyword: 현미경 대물렌즈

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Measurement of Spherical Aberration Coefficient of the Objective Lens in KBSI-HVEM (KBSI-HVEM 대물렌즈의 구면수차 계수 측정)

  • Kim, Young-Min;Shim, Hyo-Sik;Kim, Youn-Joong
    • Applied Microscopy
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    • v.37 no.2
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    • pp.111-121
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    • 2007
  • Coefficient of spherical aberration of the objective lens in the KBSI-HVEM was evaluated by diffractogram method. Instrumental resolution was also discussed with this method. In order to improve the accuracy, digital processing and graphical curve fitting for intensity profile of diffractogram were employed. Experimental concerns where the optimal procedure of the measurement con be accomplished for this study were discussed. The spherical aberration coefficient $(C_s)$ was estimated to be $2.628{\pm}0.04\;mm$ from this study, which was almost coincident with the value of the manufacture's suggestion $(C_s=2.65\;mm)$.

Design of an Endoscopic Microscope Objective Composed of GRIN(Gradient-Index) Lens with Scanning Devices (GRIN 렌즈로 구성된 주사방식의 내시현미경 대물렌즈의 설계)

  • Kim, Keyong-Jeong;Rim, Cheon-Seog
    • Korean Journal of Optics and Photonics
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    • v.20 no.6
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    • pp.311-318
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    • 2009
  • We present an attractive real time in-vivo endoscopic microscope with a resolution of submicron, in which two kinds of optical correcting plates are inserted to eliminate higher order spherical aberration and field curvature. And, since the conventional objective lens is replaced to GRIN lenses with diameter of 1 mm, the above endoscopic microscope can be effectively utilized to invade minimally for live animals.

Twin-Image Elimination in In-line Digital Holography Microscope (In-line 디지털 홀로그래피 현미경에서 쌍둥이 상 제거연구)

  • Cho, Hyung-Jun;Yu, Young-Hun
    • Korean Journal of Optics and Photonics
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    • v.18 no.2
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    • pp.117-121
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    • 2007
  • A fundamental problem in the in-line digital holography microscope is that the real image and virtual image and zero-order image are not separated spatially. In this paper, we have eliminated the zero-order noise by an averaging method and the twin image is divided using a geometrical set-up in an in-line digital holographic microscope. The size of the virtual image depends on the distance between the objective lens and the hologram plane and on the distance between the hologram plane and the image plane. We found that the virtual image size is smallest when the distance between the objective lens and the hologram plane is equal to the back focal length of the objective lens. We could divide the virtual image and real image by controlling the distance between the hologram plane and the objective lens.

Optical Design of a Lister Objective Stable Against Chromatic Variation for 405-nm Wavelength (파장 405 nm에서 파장변화에 안정화된 Lister 대물렌즈 설계)

  • Kim, Jin-Hyung;Lee, Jong-Ung
    • Korean Journal of Optics and Photonics
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    • v.31 no.6
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    • pp.295-303
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    • 2020
  • A Lister objective of NA 0.25 and 10×, stabilized against chromatic variation for a wavelength of 405 nm is designed. We develop a new solution for stabilizing a cemented doublet that has specified axial thicknesses. Using the new method, we can easily obtain a useful design for some practical purpose. At the initial design stage, two cemented doublets corrected independently are used. The stabilizing conditions for the whole system are maintained during optimization. The final design of the Lister objective shows that the chromatic variation of EFL, BFL, and RMS wavefront errors are very small at the 405-nm wavelength, as expected.

Objective Aperture Effects for the Quantitative Analysis in Electron Tomography (전자토모그래피의 정량적 분석에서 대물렌즈 조리개의 영향)

  • Kim, Jin-Gyu;Lee, Sang-Hee;Kweon, Hee-Seok;Jeong, Jong-Man;Jeong, Won-Gu;Lee, Su-Jeong;Jou, Hyeong-Tae;Kim, Youn-Joong
    • Applied Microscopy
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    • v.38 no.4
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    • pp.285-291
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    • 2008
  • We have evaluated the effects of experimental factors on transmitted electron beam intensities for quantitative analysis in electron tomography. For the correct application of Beer's law in electron tomography, the transmitted beam intensity should reflect the net effect of mass properties on beam path. So, the any other effects of the objective aperture and the specimen holder on beam path should be removed. The cut-off effects of objective aperture were examined using Quanti-foil holey carbon film and a transmission electron microscope operated at 120 kV. The transmitted beam intensities with $30{\mu}m$ objective aperture dropped about 16.7% compared to electron beam intensities without the objective aperture. Also, the additional losses of about 14.2% at high tilt angles were occurred by cut-off effects of the objective apertures. For the precise quantitative analysis in electron tomography, the effect of the objective aperture on transmitted electron beam intensities should be considered. It is desirable that 2-D tilt series images are obtained without the objective aperture for correct application of Bee's law.

Design and Analysis of an Objective Lens for a Scanning Electron Microscope by Coupling FE Analysis and Ray Tracing (유한요소해석과 광선추적을 연계한 주사전자 현미경 대물렌즈의 설계 및 해석)

  • Park, Keun;Lee, Jae-Jin;Park, Man-Jin;Kim, Dong-Hwan;Jang, Dong-Young
    • Journal of the Korean Society for Precision Engineering
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    • v.26 no.11
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    • pp.92-98
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    • 2009
  • The scanning electron microscope (SEM) contains an electron optical system in which electrons are emitted and moved to form a focused beam, and generates secondary electrons from the specimen surfaces, eventually making an image. The electron optical system usually contains two condenser lenses and an objective lens. The condenser lenses generate a magnetic field that forces the electron beams to form crossovers at desired locations. The objective lens then focuses the electron beams on the specimen. The present study covers the design and analysis of an objective lens for a thermionic SEM. A finite element (FE) analysis for the objective lens is performed to analyze its magnetic characteristics for various lens designs. Relevant beam trajectories are also investigated by tracing the ray path of the electron beams under the magnetic fields inside the objective lens.