• Journal of the Korean Society of Food Science and Nutrition
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• v.39 no.10
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• pp.1411-1417
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• 2010

In this study, we investigated the antioxidant and antibacterial activity of methanol extracts from 6 plants which were Chrysanthemum zawadskii Herb. var. latilobum (Maxim.) Kitamura (Gu-jeol-cho), Lavandula spica L. (Lavender), Rosmainus offcinals L. (Rosemary), Cymbopogon citrates (Lemongrass), Saussureae radix (Mok-hyang), Calendular officinalis L. (Calendular). Antioxidative effects of herbal extracts were measured by polyphenols, flavonoids contents and DPPH radical scavenging activity assay. We also evaluated the antibacterial activity against Staphylococcus aureus and Escherichia coli O157:H7. Methanol extracts from Gu-jeol-cho, lavender, rosemary and lemongrass showed high polyphenols contents as well as strong DPPH scavenging activity. In particular, rosemary extract contained highest polyphenol levels as $126.69{\pm}2.62{\mu}g/mg$ compared to other herbs. As for DPPH radical scavenging activities, $IC_{50}$ values of rosemary extracts were $6.23{\pm}0.58{\mu}g/mL$. The rosemary extracts also showed higher antibacterial effects against S. aureus and E. coli O157:H7. These results indicate that rosemary could be used as natural antioxidant and antibacterial agents.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.42 no.2
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• pp.195-201
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• 2016

Hyssopus officinalis is a herbaceous plant of the genus Hyssopus. Due to its properties as an antiseptic, cough reliever and expectorant, it is commonly used as an aromatic herb and medicinal plant. This study was performed to investigate the anti-oxidative and anti-melanogenic properties of Hyssopus officinalis extracts (HE) using in vitro assays and cell culture systems. As a result, HE showed higher DPPH and ABTS radicals scavenging activity in a dose-dependent manner. Also, HE inhibited the prodution of intracellular ROS and melanin contents in B16F10 melanoma cell as well as tyrosinase activity. We also found that HE inhibit mRNA expression of MITF, tyrosinase and TRP-2 gene. These findings suggest that HE may be beneficial for preventing oxidative damage and melanogenesis of skin.

• Journal of the Korean Society of Food Science and Nutrition
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• v.29 no.2
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• pp.335-341
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• 2000

In order to search for blood anticoagulant substance from herbal extracts, we extracted with cold water (Fr. I), methanol (Fr. II) and hot water (Fr. III) from 93 commercially available herbs. The anticoagulant activity of herbal extracts was examined through the intrinsic pathway by activated partial thromboplastin time (APTT), the extriansic pathway by prothrombin time (PT) and the common pathway by thrombin time (TT). Onion, garlic, clove, fenugreek, mugwort, thyme and sage had on anticoagulant activity with intrinsic pathway and mint, italian seasoning, rosemary, turmeric, tarragon and wasabie had on anticoagulant activity with common pathway. Nobody had on anticogulant activity with extrinsic pathway. Among commerical herbs tested, clove cultivated in holland was selected because it showed the most potent anticoagulant activity among the samples investigated.

• Korean journal of food and cookery science
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• v.20 no.2
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• pp.144-150
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• 2004

This study was conducted to investigate the quality characteristics and shelf-life of tofu added with basil water extract (BWE). BWE was selected from 11 kinds of herbs based on estimations of antimicrobial activity, taste and flavor. The amount of waster was 7 times greater than that of raw soybean, BWE concentration against soybean juice was 20 mg% and GDL concentration was 0.3% as the optimal preparation conditions of tofu added with BWE (BWE-tofu). Color L＊, a＊ and b＊ values of BWE-tofu were 78.11, 0.78 and 19.10, respectively, indicating that light green color was lower in the L＊ value, and higher in the at and b＊ values compared to control products. Savory taste and overall acceptability of the tofu were higher than those of control products evaluated by sensory test. Shelf-life of the tofu at 10$^{\circ}C$ was 8 days, which was prolonged by 4 days compared to control products evaluated by pH, titratable acidity and total microbes.

• Applied Biological Chemistry
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• v.48 no.2
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• pp.161-165
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• 2005

Ethanol extracts were prepared from 40 herbs, the extracts of herbs were tested their microbial inhibition activities against Helicobacter pylori. Antimicrobial activity against H. pylori was shown by clear zone and inhibition of cell growth in 24 herbs and 26 herbs extracts. Antimicrobial activity showed the high value in ethanol extracts of Salvia officinalis, Phlomis fruticosa, Creeping Rosemarinus officinalis, Lavandula, Cymtpogan citratus, Rosemarinus officinalis, Cherry Salvia officinalis, Hypericum perforatum, Ruta graveloens, Thymus vulgaris, Oreganum vulgare and Salvia officinalis. Phenol content of herb extracts have high concentration as $134.3-533.33\;{\mu}g/ml$, respectively.

• Journal of the Korean Society of Food Science and Nutrition
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• v.32 no.5
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• pp.723-727
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• 2003

The antioxidant activities and their antioxidant compounds of a group of teas obtained in local markets were investigated. A total of 18 teas were tested for their antioxidant activities based on their ability to scavenge ABTS (2,2'-Azino-bis-3-ethylbenzothiazoline-6-sulfonic acid) cation radical and DPPH (1,1-diphenyl-2-picrylhydrazyl) free radical. The former was expressed as mg of ascorbic acid equivalents per 1 tea bag (L-ascorbic acid equivalent antioxidant capacity, AEAC) and the latter was expressed as percentage of electron donating activity (EDA%). A good correlation of AEAC and EDA was observed between the two methods. The concentrations of total polyphenolics and flavonoids in tea extracts were measured by spectrophotometric methods. Total ascorbic acid was determined via the 2,6-dicholoroindophenol titrimetric method. According to the AEAC value and EDA, black tea, brown rice green tea, green tea, herb tea and malva tea showed relatively high antioxidant activities. Polyphenolic compounds were the major naturally occurring antioxidant compounds found in teas and the high concentrations of polyphenolic compounds were observed in black tea, green tea and herb tea. Overall, six teas out of 18 teas tested in the study showed better antioxidant activities and higher amounts of total polyphenolic compounds.

• Food Science and Preservation
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• v.30 no.6
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• pp.999-1011
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• 2023

This study investigated the anti-inflammatory, trypsin activity, and antioxidant effects of 11 kinds of plant extracts to discover materials for developing optimal mixtures that improve inflammation and help digestion. Ziziphus jujuba Mill. (ZJ), Leonurus japonicus Houtt. (LJ), Scutellaria baicalensis (SB), Platycodon grandiflorum, and Aster scaber extracts had excellent anti-inflammatory effects by reducing excessive nitric oxide (NO) and tumor necrosis factor-α content in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages. The malt (MA), Pyrus pyrifolia Nakai (PP), Raphanus sativus L., Platycodon grandiflorum extracts among the 11 kinds of plant extracts had high trypsin activity. The antioxidant activity of the plant extracts was examined by the DPPH radical scavenging activity, and the SB, PE, JU, and MA extracts had high antioxidant activity. Therefore, PP, MA, ZJ, LJ, and SB were selected to develop optimal mixtures that improve inflammation and help digestion. The extract of plant mixture containing PP, MA, ZJ, LJ, and SB in the ratio 1:1:2:1:2 (w/w) significantly inhibited NO production than the extract of PP, MA, ZJ, LJ, and SB, respectively, in LPS-stimulated RAW 264.7 macrophages. The DPPH radical scavenging activity of the mixture extract was significantly higher than the extract of PP, MA, ZJ, and LJ, respectively.

• Korean Journal of Clinical Laboratory Science
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• v.50 no.4
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• pp.438-443
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• 2018

The emergence of biofilms have generated urgent alarm in clinical and medicine manufacturing fields engaged in the search for novel antimicrobials from ethno-medicinal plants. The National Institutes of Health (NIH) has estimated that 70% of all microbial infections in the world are associated with biofilms. In addition, the emergence of strains resistant to conventional antibiotics has become a serious threat to global public health. Therefore, finding alternative medicines is a major issue in the field of integrative medicine. In this study, four different herb extracts were screened for biofilm formation and the activities of Klebsiella pneumoniae. Of them, Scutellaria scordifolia Fisch. ex Schrank extracts had inhibitory effects on bacterial growth and biofilm formation. The Scutellaia scordifolia Fisch. ex Schrank extracts did not cause any cytotoxicity to L929 cells. The growth of K. pneumoniae was inhibited compared to other comparators in the experimental group containing Scutellaia scordifolia Fisch. ex Schrank. In a group of experiments with plant extracts, a maximum of 60 times the level of living bacteria was confirmed compared to the controls without the addition of the Scutellaia scordifolia Fisch. ex Schrank extracts. In a group of experiments with a significantly lower level of fluorescence extraction, differential interference contrast imaging showed that the number of K. pneumonae was reduced. These results suggest that extracts of this plant be applied as antimicrobial agents against K. pneumoniae, particularly in biofilm forms.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2020.12a
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• pp.76-76
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• 2020

최근 안전한 먹거리 생산과 치유, 교육 등 다양한 목적으로 도시농업에 참여하는 인구가 늘어남에 따라 도시텃밭의 농약, 비료 사용 등으로 인한 환경오염과 해충 피해, 농산물의 건강성 등 다양한 문제가 제기되고 있다. 따라서 도시텃밭 작물의 생육 증진, 잔류농약 문제 해결 등 농산물 안정성 증가에 기여하고자 식물추출물을 활용한 엇갈이 배추의 생육특성을 조사하였다. 선행연구 결과를 바탕으로 자원식물류 5종(쇠비름, 소리쟁이, 명아주, 박주가리, 환삼덩굴)과 허브류 5종(메리골드, 서던우드, 캐모마일, 애플민트, 로즈마리), 식용작물류 5종(양파, 마늘, 부추, 차즈기, 들깨) 등 추출 대상 식물 15종을 60℃에서 3일간 건조시켜 마쇄하였다. 이후 각 식물체 100g을 35% 에틸알코올 1,000ml에서 7일간 추출하였다. 추출물은 30%로 희석하여 주 2회씩 총 3주간 배추에 엽면살포하였다. 엽면적, 엽수, 지상부 및 지하부 생체중 등 생육특성을 7일 간격으로 조사하였으며 그 결과는 다음과 같다. 엽면적과 엽폭은 환삼덩굴 처리구에서 104.58cm²와 9.80cm로 가장 넓었고, 엽장은 박주가리 처리구에서 25.24cm로 가장 길었으며, 지하부 생체중은 메리골드 처리구에서 6.24g으로 가장 높았다. 엽수는 애플민트와 마늘 처리구에서 6.4개로 가장 많았고, 지상부 생체중과 엽록소는 집단 간 유의한 차이가 나타나지 않았다. 전반적으로 무처리 대비 엽면적 22%, 엽폭 18%의 유의한 생장 차이를 보인 환삼덩굴 추출물과 엽면적 12%, 엽장 17%의 생장 차이를 보인 박주가리 추출물, 엽면적 10%, 엽폭 9%의 생장 차이를 보인 양파 추출물이 엇갈이 배추 생육에 효과적인 것으로 나타났다. 본 연구결과 환삼덩굴, 박주가리, 양파 등 일부 식물추출물이 엇갈이 배추의 생육을 증진시킬 수 있는 것으로 나타나 주말농장 등 도시텃밭에 활용할 수 있을 것으로 사료되었다. 향후 식물추출물의 효과성을 증진시키기 위해 추출 방법과 적정 농도, 보관 시기 등에 대한 연구와 함께 도시텃밭 해충방제 효과를 알아보기 위한 약해검정과 살충효과를 분석할 필요성이 있다.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.45 no.1
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• pp.77-85
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• 2019

OMiYakSung theory consists of various herbs that contain at least one or more of the five flavors. This theory has been used to prevent human diseases and enhance the immune system. The main objective of the present study was to investigate efficacy differences and changes in ingredients of blended and single herb extracts based on OMiYakSung theory. We selected three herbs Coptis japonica Makino, Cimicifuga heracleifolia Komarov, and white Poria cocos and assessed their physiological effect. As results, the blended extracts showed excellent cell migration effect at 400 ug/mL concentration, compared to the single extract. In addition, the blended extracts enhanced immune function by increasing the activity of dendritic cells and showed the highest antioxidant activity by DPPH assay and HPLC-ABTS assay. In this study, we developed a new materials that can be applicable to cosmetics and pharmaceuticals field by applying oriental medicine theory.