• Title/Summary/Keyword: 핵형 분석

Search Result 198, Processing Time 0.027 seconds

Systematic Study on the fishes of the Family Cobitidae (Pisces, Cypriniformes) 4. The Analyses of Karyotype and Mitochondrial DNA between the Two Species of the genus Misgurnus from Korea (기름종개과(Family Cobitidae) 어류의 계통분류에 관한 연구. 4 미꾸리속 어류 2종의 핵형 및 mtDNA 분석)

  • 이혜영;양서영
    • The Korean Journal of Zoology
    • /
    • v.37 no.3
    • /
    • pp.439-451
    • /
    • 1994
  • 미꾸리속 어류 2종의 유전적 차이를 알아보기 위하여 염색체 분석과 미토콘드리아 DNA(mtDNA) 분석을 실시하였다. 일반염색에 의한 핵형 분석 결과 미꾸리(2N=50)와 미꾸라지(2N=48)는 염색체수에 차이가 있었으며. N-banding 분석 결과 두 종간에는 인형성 부위의 위치와 크기에 차이가 있었다. C-handing 겪과 미꾸라지는 1번 염색체쌍 동원 체부위에 넓게 C-band플 갖고 있었다. 미꾸리속 어류 2종의 mtONA를 7개의 6-base cutting 제한효소로 처리하여 절편 양상을 비교. 분석한 결과 2종 공히 mtDNA의 genome 크기는 약 16.OKb였으며 fragment homology(F)에서 미꾸리의 종내 집단간의 F값은 0.674. 미꾸라지는 0.862로 유사하게 나타났으나, 종간 F값은 0.207(0.074-0.417)로 낮았다 염기치환율은 미꾸리가 p=0.021, 미꾸라지는 p=0.002로 미꾸라지가 미꾸리에 비해 매우 낮은 염기치환율을 보였고. 종간 평균 염기치환율은 p=0.104로 차이 를 나타냈다. MtDNA 분석과 핵형 분석 결과 미꾸라지는 Robertsonlan translocation의 결과 미꾸리로 부터 분화된 것으로 추정 되었다.

  • PDF

Cytogenetic Analysis of Seven Angelica Species (Angelica속 식물 7종의 세포유전학적 분석)

  • Choi, Hae-Woon;Koo, Dal-Hoe;Lee, Woo-Kyu;Kim, Soo-Young;Sung, Jung-Sook;Seong, Nak-Sul;Suh, Young-Bae;Bang, Jae-Wook
    • Korean Journal of Medicinal Crop Science
    • /
    • v.13 no.3
    • /
    • pp.118-121
    • /
    • 2005
  • Karyotypes were established in seven Angelica species cultivated in Korea. The somatic chromosome numbers were 2n = 2x = 22 with the basic number of x = 11 in all Angelica plants examined. Their metaphase chromosomes ranged from 3.56 ${\mu}M$. to 8.91 x. in length. Distinctive Karyotypes were found in two species, A. tenuissima with all metacentries, K(2n) = 2x = 22m, and A. genuflexa with all subtelocentrics, K(2n) = 2x = 22st. Karyotype formulas of A. gigas, A. acutiloha, A. sinensis, A. decursiva and A. dahurica were K(2n) = 2x = 20m + 2sm, K(2n) = 2x = 12m + 10sm, K(2n) = 2x = 16m + 6sm, K(2n) = 2x = 18m + 4sm and K(2n) = 2x = 10m + 10sm + 2st, respectively. Cytological data showed that chromosomal polymorphisms within species were observed in Angelica plants compare to other regions.

A comparative Karyotype study on Korean Squirrels. II. Karyotype Analysis of Sciurus vulgaris coreae and Tamias sibiricus asiaticus by G-banding Method. (한국산 다람쥐 핵형의 비교연구 II. G-banding 방법에 의한 한국산 청서(Sciurus vulgaris coreae)와 다람쥐(Tamias sibiricus asiaticus)의 핵형분석)

  • 김종봉;이희영
    • The Korean Journal of Zoology
    • /
    • v.33 no.3
    • /
    • pp.255-259
    • /
    • 1990
  • The Karyotypes of Korean Sciurus uulgaris coreae and Tamias sibiricus asiaticus were analyzed by the G-banding method. Chromosomes of two species could be identified by G-banding patterns. The banding patterns of chromosomes 9, 10, 12 and X of S. vulgaris coreae were identical to those of chromosomes 6, 9, 12 and X, respectively of T. sibiricus asiaticus. It was shown that chromosomes 4, 10, 7 and 17 of T. sibiricus asiaticus resulted from pericentric inversion of chromosomes 1, 7, 8 and 16 of S. vulgaris coreae. These results suggested that pericentric inversion was an important factor in the karyological differentiation of two species.

  • PDF

Comparative Karyological Analysis of the Korean Treefrogs, Hyla japonica and Hyla suweonensis (Anura, Hylidae) (한국산 청개구리 두 종(Hyla japonica와 H.suweonensis)의 핵형 비교분석)

  • 이혜영;유성림
    • The Korean Journal of Zoology
    • /
    • v.31 no.2
    • /
    • pp.104-110
    • /
    • 1988
  • The comparative karyological analysis of the Korean treefrog, Hyla japonica and Hyla suweonensis performed by conventional giemsa-staining and NOR-staining method. The karyotypes of both species were the same (2n=24). However, according to the Karyological analysis, the 6th chromosomes of the two species distinctly different. Eventhough the 6th chromosomes of the two species shown the same % length, construction of the 6th chromosome of H.japonica was subtelocentric chromosome while H. suweonensis was submetacentric chromosome. This phenomena could explain that the morphological differences in the 6th chromosomes might be caused by pericentric inversion. The two species have 1 pair of NOR site in the 6th chromosome.

  • PDF

Karyotype of Korean Birds VI. Karyological Anaiysis on Two Species of the Genus Dendrocopos by C-Banding Method (한국산 조류의 핵형 VI. C-Banding방법에 의한 딱다구리의 두 종의 핵형분석)

  • 이혜영;이성근;유성림
    • The Korean Journal of Zoology
    • /
    • v.33 no.2
    • /
    • pp.217-221
    • /
    • 1990
  • The chromosomal analyses of Dendmcopos major hondoensis and D. leucotos Ieucotos(Picidae:Piciformes) in Korea were performed by conventional Giemsa staining and C-banding method. The diploid number of two species was 2n=90-92 and arm number was AN= 92-96.The conventional karyotypes were very similiar but distribution of constitutive heterochromatin were differ in the first chromosome. The second and several pairs of macro-telocentric chromosbmes have telomeric constitutive heterochromatin.

  • PDF

Electrophoretic karyotype of Flammulina velutipes and its variation among cultivars (팽이버섯의 핵형분석과 균주 사이의 핵형 다양성)

  • Lee, Song Hee;Lee, Mi-Kyoung;Kim, Na-Ri;Lee, Chang-Yun;Lee, Hyun-Sook
    • Journal of Mushroom
    • /
    • v.12 no.1
    • /
    • pp.63-66
    • /
    • 2014
  • The karyotype of F. velutipes Korean cultivar, Fv 3-6, was compared with those of Japanese cultivars, Fv 0-1, Fv 1-5, Fv 11-1, by CHEF gel electrophoresis. The Korean cultivar, Fv 3-6, showed the difference from the three Japanese cultivars in number and size of chromosomes; the Fv 3-6 had two and one more chromosomes then Fv 0-1 and Fv 11-4, and Fv 1-5 had, respectively. The karyotyping by CHEF gel electrophoresis is quite suitable to define new Korean cultivars against Japanese cultivars.

Speciation Mode Reconstruction for Lepilemur six species (L. mustelinus, L. edwardsi, L. dorsalis, L. leucopus, L. ruficaudatus, L. septentrionalis) Based on the Lepilemur Karyotype Analysis (여우원숭이속(Lepilemuridae)의 핵형 분석을 통해 나타난 Lepilemur 6종(L. mustelinus, L. edwardsi, L. dorsalis, L. leucopus, L. ruficaudatus, L. septentrionalis)의 종 분화 양상)

  • 정기윤
    • Journal of the Korea Academia-Industrial cooperation Society
    • /
    • v.5 no.2
    • /
    • pp.141-149
    • /
    • 2004
  • The aim of this study was to test the validity of the hypothesis that the karyotypes of four species of Lepilemuridae were formed spontaneously from their ancestral hybrid karyotype. Hypothetical ancestral haploid Karyotype of Lepilemuridae is composed of 18 autosomes and X chromosomes. Lepilemur mustelinus karyotype has four tandem fused chromosomes and one Robertsonian translocated chromosome pairs. Lepilemur septentrionalis septentrionalis karyotype has only two pairs of translocated chromosomes. We reconstruct and suggest ancestral karyotype of LMU(ancLMU) and LSS(ancLSS), from which all four studied species were derived. Hybrids of ancLMU and ancLSS were formed and produce differently fused equilibrated gametes via circular form arrangement during gametogenesis. Five unit of trivalent homologous chromosome pairs were engaged in a circular form to give new gamete corresponding to the karyotype of L. dorsalis, orientation of one unit of trivalent was inversed in the circle to gave new gamete corresponding to the karyotype of L. leucopus. Seven homologous chromosome pairs were engaged in circular form to give haploid karyotype of Lepilemur ruficaudatus. Only one homologous chromosome pair is dissociated and the other chromosome pairs rearranged in the circle to form haploid karyotype of Lepilemur edwardsi. The new gametes could be produced from these circular forms. When the new gamete fertilized with the same type of gamete, The new homozygote is produced as existing L. dorsalis, L. leucopus, L. edwardsi and L. ruficaudatus. These results support the theory that new species could be formed in hybrid population through activated chromosome fusion, chromosome rearrangement in circular from at zygotene stage and production of equilibrated gametes to form homozygote new species.

  • PDF

Karyotypes of Five species in Odontobutidae and Cottidae of Korea (한국산 동사리과 (Odontobutidae)와 둑중개과 (Cottidae) 5종의 핵형)

  • Park, Gab Man;Song, Ho Bok
    • Korean Journal of Ichthyology
    • /
    • v.18 no.3
    • /
    • pp.155-162
    • /
    • 2006
  • The chromosome numbers of five species in two families of Korea are investigated: Odontobutis platycephala, O. interrupta, and O. obscura in Odontobutidae, and Cottus koreanus and C. hangiongensis in Cottidae. In Odontobutis species, the three species showed the diploid chromosome number, 2n=44 (NF=44) telocentric chromosomes. In Cottus species, the mitotic chromosomes from 24 groups with two chromosomes each indicated that it is a diploid. The karyotype of C. koreanus and C. hangiongensis is 2n=48 and NF=52. These species is the first report on the chromosomes and the karyotype analysis except O. platycephala. Our findings provide cytotaxonomic evidence for the species distinctness of these five species whose descriptions were based primarily on external morphology.

한국산 관박쥐(Rhinoiophus terrumequinum)의 핵형분석에 의한 분류학적 연구

  • 이정훈;손함원
    • The Korean Journal of Zoology
    • /
    • v.31 no.4
    • /
    • pp.309-317
    • /
    • 1988
  • 내륙산 관박쥐와 제주산 관박쥐간의 동-종 여부를 조사하기 위하여 경남과 제주에서 채집한 이들 관박쥐들을 핵형분석하였다. 핵형분석의 결과, 2종 모두 염색체수는 2n=58이었으며 FN도 62였다. 상염색체에서는 중형의 2쌍과 미세형 1쌍이 중부염색체(metacentrics)였고, 대형에서 소형까지는 25쌍이 단부염색체facrocentrics)이었다. 성염색체에서 X염색체는 대형의 차중부염색체(submetacentrics)였고, Y염색체는 소형의 단부염색체(acrocentrics)이었다. 그리고 이들 염색체들은 동원체 부근에 이질염색체을 가지는 특이한 1쌍의 단부염색체(acrocentrics)가 존재하고 있었다. in this study, We analysed the karyotypes of the inland bat(Rhinolox)thus femequinum hora) and the Cheju-Island bat(Rhinorophus ferrumequinum quelpartis(\ulcorner)) collected in Kyungnam and Cheju provinces to identify the homogeneous between them. The results are as follows. The diploid number of chromosomes of them are equally 58 and the fundamental number 62. In the autosomes, metacentrics consist of two pairs of the middle form and a pair of the micro-form. And acrocentrics have 25 pairs of large and small form. In sex-chromosomes, X-chromosome is a large submetacentrics and Y is a small acrocentrics. And, these chromosomes possess a pair of particular acrocentrics having heterochromatin around centromere in both the inland bat(Rhinolophus fenmequinum korai ) and Cheju-Island bat(Rhinolophus fewmequinum querporis (\ulcorner)).

  • PDF

Karyotype Analysis of Anemarrhena asphodeloides Bunge (지모의 핵형 분석)

  • Kim, Soo-Young;Koo, Dal-Hoe;Bang, Jae-Wook
    • Korean Journal of Medicinal Crop Science
    • /
    • v.10 no.2
    • /
    • pp.144-146
    • /
    • 2002
  • Cytogenetic analysis of Anemarrhena ashodeloides BUNGE, which is one of medicinal plants belonging to Haemodoraceae was carried out using Feulgen staining. The somatic metaphase chromosome number was identified to 2n=22 (x=11) and the size of chromosomes ranges from $1.27-3.80\;{\mu}m$. Three pairs of chromosomes were relatively long in total length and the others were short. The karyotype was bimodal in chromosome length and arm ratios. The chromosome complement comprise eight pairs of metacentric (chromosome 2, 3, 6, 7, 8, 9, 10, and 11), two pairs of submetacentric (chromosome 4 and 5), and one pair of subtelocentric (chromosome 1).