• Title/Summary/Keyword: 해산어 양식장

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Effects of Suspended Solids, pH and Salinity on the Chemical Fate of Oxolinic Acid in the Aquatic Environment (해양환경에서 부유물질, 염분 및 pH의 옥소린산 화학적 거동에 미치는 영향)

  • Yoon Duk-Hyun;Kim Mu-Chan
    • Journal of the Korean Society of Marine Environment & Safety
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    • v.12 no.2 s.25
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    • pp.99-106
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    • 2006
  • The fate of chemical pollutants in the aquatic environment is generally considered to be strongly influenced by environmental factors such as pH, salinity and electrostatic charges on the surface of particles ai well as by the characteristic of chemicals. Oxolinic acid was measured by chemical analysis using HPLC to determine the effect of salinity, pH and suspended solids on chemical binding and by bioassay for measuring bioactivity. The higher contentration of suspended solids in the medium, the lower concentration of oxolinic and was detected in measurements from by both HPLC and biosssay analysis. This indicates particle may have a stronger binding or absorption effect on oxolinic acid. Bioassay analysis showed weaker bioacivity at higher salinity and pH 7.0, but this result of bioassay analysis was different from the result of HPLC.

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Detection and Analysis of the Potential Risk of VHSV in Bivalves in Korea (한국 이매패류 내 VHSV 검출 및 잠재적 위험성 분석)

  • Choi, Jae Chan;Kim, Young Chul;Choi, Hwan Jun;Park, Jeon Oh;Jeong, Hyun Do
    • Journal of fish pathology
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    • v.28 no.1
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    • pp.27-35
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    • 2015
  • VHSV is a major viral agent that affects freshwater and marine fish, causing serious economic losses in aquaculture in the world. Due to their filter-feeding activity, bivalve mollusks may act as viral transmitters after accumulation of the fish viruses released into seawater from infected fish. Amplification by RT-PCR was carried out to investigate the presence of VHSV in pacific oysters (Crassotrea gigas) and blue mussels (Mytilus edulis), inhabiting regions around aquatic farms in Korea. Primers designed from conserved regions of VHSVs allowed us to detect four different types of VHSV in a single PCR. Twenty two of the eighty four samples showed positive results of VHSV in a 2-step RT-PCR. Using six positive samples from three different regions in Korea, we cloned and sequenced the glycoprotein (G) gene (467-bp long) of VHSVs. Genetic analysis of the VHSVs detected in shellfish in various geographical areas of Korea showed highly restricted results to VHSV type Iva. This was in agreement with the reports showing only a single genotype of VHSV (Iva genotype) in outbreaks in cultured or wild fish in Korea. Consequently, we investigated VHSVs carried by bivalve mollusks inhabiting the vicinity of aquatic farms, and revealed correlationship between the type of viral accumulated in shellfish by filter-feeding, and those detected in disease outbreaks in fish.

참돔 노지종묘생산과 요각류 번식을 위한 적정 시비제

  • 방인철;박상용;권성민;신현웅
    • Proceedings of the Korean Aquaculture Society Conference
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    • 2003.10a
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    • pp.3-63
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    • 2003
  • 우리나라 서해안의 폐염전 또는 대하양식장을 이용한 해산어 종묘생산이 조피볼락, 감성돔, 숭어 등을 대상으로 이루어지고 있다. 이렇게 생산된 종묘는 기형개체가 전혀 없는 건강 종묘이며, 생산 원가 또한 육상수조식에 비해 최고 5배정도 낮다. 한편 참돔은 육상수조에서 종묘생산시 기형률이 비교적 높게 나타나므로 노지를 이용한 종묘생산이 요구되고 있다. 현재 노지 종묘생산시 요각류 번식을 위한 시비제로는 암모니아, 인산염, 칼륨 등이 함유되어 있는 화학비료(요소, 복합비료)나 계분, 돈분, 우분 등의 유기질 비료가 주로 사용되고 있다. 그러나 화학비료를 사용시 급격한 수질변화로 요각류의 폐사가 발생하고, 매년 지속적인 생산량을 기대하기가 어려운 문제점이 있다. 따라서 적절한 시비제를 구명하기 위하여 유기질 비료(알팔파, 숙성계분)와 화학비료(요소, 복합)를 단독 또는 병행 사용하여 2개월간의 동ㆍ식물플랑크톤의 변동량과 치어의 생존율을 조사한 결과, 유기질 비료는 부화자어가 입식된 이후에도 연속하여 시비하여도 생존율에 영향을 미치지 않았으나, 화학비료의 경우 일정 농도 이상에서는 동ㆍ식물플랑크톤의 번식에 장애를 초래하였고 이에 따라 자어가 전량 폐사하였다. 실험군의 생존율은 육상수조에서 40%, 알팔파와 숙성계분을 시비한 실험군이 15%, 요소, 복합, 알팔파를 시비한 실험군이 10%, 요소, 복합, 숙성 계분을 시비한 실험군이 8%, 요소와 복합을 시비한 실험군은 0%를 나타내었다. 또한 기형률에 있어 노지 실험군은 0.7%로 매우 낮게 나타났으나, 육상수조 실험군은 61%에 해당하는 치어가 기형이었으며, 그 형태도 다양하게 나타났다.fer, Artemia sp., copepods, 부화 25일째부터는 copepods였다. 초기성장은 부화직후 전장 1.95 mm였던 것이 10일째는 2.96 mm, 20일째는 3.89 mm, 30일째는 12.9 mm, 50일째는 35.1 mm를 나타내었다. 그러나 말쥐치 자ㆍ치어가 성장함에 따라 발생하는 지속적인 폐사와 종묘의 수송시 발생하는 폐사를 줄일 수 있는 방법이 추후에 해결되어야 할 것으로 사료된다.8,000~215,000 마리의 자어를 낳아서 개체에 따라 큰 차이를 나타내었다. 1999년에는(어미 : 전장 20.7~25.5cm, 체중 205.5~396.4g) 1 회 출산때마다 7,000~50,000 마리의 자어를 출산하였다. 3. 출산간격. 다회 출산한 쏨뱅이 어미가 첫 번째 출산에서 다음 출산까지 소요되는 기간을 조사한 결과 13일에서 25일이 걸리는 것으로 나타났다. 특히 4회 출산 한 1998년 쏨뱅이 어미는 첫 번째 출산 후 19일만에 두 번째 출산이 이루어 졌고 각각 13 일과 14 일만에 세 번째, 네 번째 출산이 이루어져 총 46일 동안 4회에 거쳐 자어를 출산하였다. 4. 출산자어의 크기. 1997년 3회 출산한 쏨뱅이의 출산시기별 자어 크기를 조사한 결과 첫 번째 출산에서는 평균전장 4.1$\pm$0.07mm의 자어를 낳았으나 두 번째 출산에서 자어의 크기는 평균전장이 3.9$\pm$0.11mm, 세 번째 3.8$\pm$0.12mm로 점차 작은 경향을 나타내었다..27mm(평균 1.76mm, n=20) 그리고 Snout length 0.74~l.67mm(평

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A New Rhabdovirus (HRV-like) Isolated in Korea from Cultured Japanese Flounder Paralichthys olivaceus (양식산 넙치로부터 HRV-like Rhabdovirus의 분리)

  • Oh, Myung-Joo;Choi, Tae-Jin
    • Journal of fish pathology
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    • v.11 no.2
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    • pp.129-136
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    • 1998
  • In March 1997, a new rhabdovirus was isolated from moribund cultured Japanese flounder Paralichthys olivaceus in sea water tank and cage culture systems in Kyung-Nam and Chun-Nam province, Korea. At temperature $15^{\circ}C$ the virus replicated and induced cytopathic effects (CPE), which progressed to eventual cytolysis, in susceptible cell lines, including RTG-2 and EPC. The CHES-214 cell line was refractory. Virus particles were bullet-shaped and measured $70nm{\times}100$ to 150 nm in size. The isolate was sensitive to pH 3, to diethyl ether, and to heat ($50^{\circ}C$ 5 min, $60^{\circ}C$ 1 min). Viral replication was not inhibited by $10^{-4}$ M 5-iododeoxyuridine. Virus infectivity was reduced by anti-HRV (8401-H) rabbit serum, but can not reduced by antisera against infectious hematopoietic necrosis virus (IHNV), chum salmon reovirus (CSV), retrovirus of salmonid (RVS) and infectious pancreatic necrosis virus (IPNV). HRV virus antigen was detected by fluorescent antibody test (FAT) in the cytoplasm of infected EPC cell. Purified isolates virions were composed of: polymerase (L), glycoprotein (G), nucleoprotein (N) and 2 matrix proteins (M1 and M2). Based upon their relative mobilities, the estimated molecular weights of the proteins were: L, 160 kDa; G, 55 kDa; N, 45 kDa; M1, 26 kDa; and M2, 22 kDa.

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Acute Toxicity of Ozone on Survival and Physiological Conditions of Olive Flounder, Paralichthys Olivaceus (넙치(Paralichthys olivaceus)의 생존과 생리상태에 미치는 오존의 급성 독성)

  • Kim, Heung-Yun;Oh, Myung-Joo;Jung, Sung-Ju
    • Journal of fish pathology
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    • v.12 no.1
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    • pp.32-41
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    • 1999
  • Ozonization for rearing seawater in land-based culture system has recently been utilized for disinfection of pathogenic microorganisms and improvement of water quality. This study was conducted to examine the effects of total residual oxidants (TRO) in ozone-treated seawater on survival, blood parameters, osmolality and oxygen consumption, and gill tissue of the flounder. Paralichthys olivaceus. Experiments were carried out with the starved flounder of 12~19 cm in total length at $20^{\circ}C$. The 48-hr and 96-hr $LC_{50}$ for the flounder amounts to 26.4 ppb and 22.3 ppb, respectively. With increase of TRO concentration from 24 to 39 ppb, the values of hematocrit, hemoglobin concentration, red blood cell count and osmolality of the flounder with respect to exposure time were significantly elevated, however, the oxygen consumption rates decreased. In the case of the fish exposed to 13 ppb for 96 hrs, blood glucose increased with an elapse of exposure time, while survival rate was 100%. Death apparently resulted by massive destruction of gill lamellar epithelium, severe osmotic imbalance and the lack of oxygen uptake. The results of this experiment indicated that to protect aquaculture organisms, the ozone-treated seawater should not contain any residual oxidants, and that relatively long-term exposure to TRO of low concentration can impact on survival and physiological conditions of the flounder.

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