• 대한본초학회지
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• 제26권4호
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• pp.125-132
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• 2011

Objectives : An aim of study is to investigate effects of curculiginis rhizoma in vitro (factor Xa (FXa) inhibitor assay, prothrombinase assay, prothrombin time (PT) assay, activated partial thromboplastin time (aPTT) assay) and in vivo experiment (blood clotting time, thromboxane B2 content assay in serum and weight of thrombus by AV-shunt rat model). Methods : We gained a human serum and used serum in vitro study such as factor X activity (FXa) inhibition, prothrombinase inhibition, prothrombin time (PT) and activated partial thromboplastin time. Fifteen SD rats were divided into three groups (intact control group and two experimental group treated with extract of Curculiginis Rhizoma(ECR)). Rats were orally administrated DW (intact control group), 600 mg/kg concertration of ECR and 200 mg/kg concertration of ECR. After one hour, we anesthetized rats and made arteriovenous (AV) shunt rat models to study weights of thrombus, took a hole blood to study content of thromboxane B2 and blood clotting time. Results : In vitro, ECR increased a inhibitory activity of FXa, prothrombinase and aPTT compared than intact control group. Especially ECR made significant increase of FXa and prothrombinase inhibitory activity (p<0.05, p<0.01). And PT were increased in ECR control group compared with intact control group. In vivo, a blood clotting time of experiment group treated with ECR 600 mg/kg were significantly increased compared with that of intact control group (p<0.05) and content of thromboxane B2 was significantly decreased in group treated with ECR 600 mg/kg in seum. The weight of thrombus were significantly reduced in group treated with ECR 600 mg/kg compared with intact control group (p<0.05). But in vivo experiment study, those of group treated with ECR 200 mg/kg were reduced compared with those of intact control group without statistical significance. Conclusions : ECR has a antithromboic activity in internal course with inhibitory activity of FXa and prothrombinase in vitro, it required to research more study for effective compounds.

• 대한화장품학회지
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• 제36권4호
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• pp.241-251
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• 2010

최근 몇 년 동안 탈모로 인해 우울증을 경험하는 인구가 증가하고 탈모 관련 치료법도 임상의학이나 외과적인 수술법과 같이 전문화되고 있다. 탈모 방지와 발모를 촉진하기 위한 시술법과 소재들이 있으나 그 효능, 효과가 뛰어나지 못하고 일부는 부작용을 동반하는 경우가 있다. 본 총설의 마이크로니들 시술법은 약물의 흡수를 촉진시켜주는 매우 작고 섬세한 니들을 이용한 시술법이다. 이 시술을 하는 동안 마이크로니들이 두피 내부로 약물의 흡수를 도와주는 마이크로 홀을 만들어 준다. 마이크로니들 시술 약물의 주요성분으로 대두 발효물질을 분석하였으며 대두 발효 소재는 탈모증에 항산화, 항안드로겐, 항혈전의 기능을 가진다. 대두 발효물의 흡수를 증가시켜주는 마이크로니들의 우수한 약물전달시스템으로 인하여 대두 발효물이 마이크로니들과 함께 사용될 때 복잡한 탈모기전에 더욱 효과적이다. 이 시술법은 탈모증을 방지하고 관리하며 개선시켜줄 수 있는 매우 유용한 두피케어법이며 일반적인 두피관리법 보다 더 빠른 효과를 나타낼 수 있어 차별화된다. 대두 발효물을 이용한 마이크로니들 시술법은 두피케어를 위한 진일보한 기술이다.

• 대한한방부인과학회지
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• 제19권2호
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• pp.1-17
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• 2006

Purpose : The Purpose of this research was to investigate the effects of antithrombotic activities of Haenggyonghonghwatang (HGHHT). Methods : Measured the effect which was given to blood flow rate through the regular volume of glass tube after the blood was diluted five times with ACD soulution. Antithrombotic effect was calculated as a percentage of the experimental animal figure protected from the paralysis of hind legs or death of the mouse that is caused from the administration of platelet aggregation regent. Each of the groups consisted in 8 mice, was divided into Normal, Control, and HGHHT. All of these 3 group were supplied a saline solution and after an hour the control group brought the dextran extravasated blood. Also the HGHHT group was dosed to the experimental mice with Oral Zonde one day before the experiment. After that, the mice were abstained from food. And then we gave a measured amount of it before an hour. Finally, it gave rise to dextran extravasated blood as well as the Control group. Results : The results were obtained as follows. HGHHT inhibited platelet aggregation induced by ADP and epinephrine significantly as compared with the control group. HGHHT showed fibrinolytic activity insignificantly as compared with the control group. HGHHT increased blood flow rate significantly as compared with the control group in vitro, but insignificantly as compared with the control group in vivo. HGHHT inhibited pulmonary embolism induced by collagen and epinephrine(inhibitive rate 50%). HGHHT increased number of platelet, fibrinogen amount and shortened prothrombin time, activated partial thromboplastin time significantly as compared with the control group in thrombus model induced by dextran. Conclusion : HGHHT is effective antithrombotic activity from experimental result.

• 폴리머
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• 제37권4호
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• pp.411-419
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• 2013

다양한 생체재료들이 이식용 인공장기나 의료용 장비로 폭넓게 사용되고 있으나 혈액과 접촉하는 경우가 많아짐으로써 발생되는 혈전의 문제로 인해 이식재와 혈액간의 혈액 친화성의 향상이 연구자들에게 관심의 대상이 되고 있다. 연잎의 표면구조는 항 오염 특성이라는 측면에서 많은 연구가 진행되어 왔으며, 산업적인 용도로 적용하고자 하는 주된 시도들이 있어 왔다. 대부분 주형법이나 졸-젤 방법, 층 쌓기 방법 등을 포함한 다양한 기법으로 표면처리를 함으로써 인위적인 모사가 가능해 왔다. 최근에 들어 이러한 표면의 초소수성 성질이 의료용 재료의 표면처리 기법으로써 혈관 이식재에서부터 항 박테리아용 표면에까지 널리 적용하려는 시도가 진행되고 있다. 본 리뷰논문에서는 최근 많이 사용되는 연잎 구조로의 표면처리 기법들을 중심으로 요약하였으며, 이들을 이용한 의료분야로의 적용 시도들을 정리하고자 하였다.

• 한국미생물·생명공학회지
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• 제43권4호
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• pp.373-377
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• 2015

마가목의 열매인 마가자는 생식하거나 다류, 발효주로 제조되어 음용되며, 한방에서는 고혈압 및 관절염 치료 등에 사용되고 있다. 본 연구에서는 현재까지 보고되지 않은 마가자의 항혈전 활성을 평가하기 위해 울릉도 마가자의 ethanol 추출물 및 이의 순차적 유기용매 분획물을 조제하고 이의 혈액응고 저해 및 혈소판 응집저해 활성을 평가하였다. 그 결과, 마가자의 ethanol 추출물의 ethylacetate 분획에서 강력한 트롬빈, 프로트롬빈, 혈액응고인자 저해와 함께, 혈소판 응집저해 활성을, hexane 분획에서는 아스피린보다 강력한 혈소판 응집저해 활성을 확인하였다. 또한 마가자의 추출물 및 분획물들은 0.5 mg/ml 농도까지 인간 적혈구 용혈활성이 없음을 확인하여, 마가자의 활성분획물이 신규의 항혈전제로 사용 가능함을 제시하였다.

• 동의생리병리학회지
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• 제20권4호
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• pp.957-965
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• 2006

This study was peformed to evaluate antithrombotic activities and anti-inflammatory effects of Kami-BoyangHwanoh-Tang(KBHT). The major findings were summarized as follows. In experiment of anti-thrombotic effect; KBHT inhibited human platelet aggregation induced by ADP and epinephrine as compared with the control group and inhibited pulmonary embolism induced by collagen and epinephrine (inhibitory rate is 50 %). KBHT increased platelet number significantly and also KBHT shortened PT and APTT significantly as compared with the control group in thrombus model induced by dextran. In experiment of anti-inflammatory effect; KBHT inhibited $IL-1{\beta}$, IL-6, $TNF-{\alpha}$, COX-2 and NOS-II mRNA expression as compared with the control group in a concentration-dependent degree, and inhibited NO production significantly at 50, $100\;{\mu}g/^{ml}$, and also inhibited ROS production in a concentration-dependent degree as compared with the control group in RAW 264.7 cell line. KBHT inhibited $IL-1{\beta}$, IL-6 and $TNF-{\alpha}$ production significantly in serum of acute inflammation-induced mice, and decreased $IL-1{\beta}$, IL-6 and $TNF-{\alpha}$ production in spleen tissue, and also decreased IL-6 and $TNF-{\alpha}$ production in liver tissue, but increased $IL-1{\beta}$ production in liver tissue of acute inflammation-induced mice. KBHT increased survival rate at 3rd day in mice with lethal endotoxemia induced by LPS. These results suggest that KBHT can be useful in treating diverse female diseases caused by thrombosis and inflammation such as endometrosis, myoma, pelvic congestion, chronic cervicitis, chronic pelvic inflammatory disease and so on.

• 대한한방부인과학회지
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• 제20권3호
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• pp.45-64
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• 2007

Purpose: This study was performed to evaluate anti-thrombotic and anti-inflammatory effects of NeungaSoJeokTang water extract (NSJT). Methods: In the study of anti-inflammatory effects, NSJT was investigated using cultured cells and murine models. As for the parameters of inflammation, levels of several inflammatory cytokines and chemical mediators which are known to be related to inflammation were determined in mouse lung fibroblast cells(mLFC) and RAW 264.7 cells. Results: Prior to the experiment, we evaluated sGOT, sGPT, BUN and creatine after the treatment. As a result, NSJT was innoxious on liver and kidney. In experiment of anti-thrombotic effect, NSJT inhibited the platelet aggregation induced by ADP and epinephrine, and inhibited pulmonary embolism induced by collagen and epinephrine. NSJT did not affect significantly the blood flow rate both in vitro and in vivo. NSJT increased platelet number and fibrinogen amount, and NSJT shortened PT and APTT in thrombus model induced by dextran. In experiment of anti-inflammatory effect, NSJT inhibited $IL-1{\beta}$, IL-6, $TNF-{\alpha}$, COX-2 and NOS-II mRNA expression in a concentration-dependent manner in RAW 264.7 cell line, and inhibited significantly NO production at 50, 100 ${\mu}g/ml$, and also inhibited ROS production in a concentration-dependent manner. NSJT inhibited $IL-1{\beta}$, IL-6 and $TNF-{\alpha}$ production significantly in serum of acute inflammation-induced Balb/c mice, and decreased $IL-1{\beta}$, IL-6 and $TNF-{\alpha}$ production in spleen tissue, but increased $IL-1{\beta}$, IL-6 and $TNF-{\alpha}$ production in liver tissue. NSJT increased survival rate at the 3th day in ICR mice with lethal endotoxemia induced by LPS. Conclusion: These results suggest that NSJT can be used for treating diverse female diseases caused by thrombosis and inflammation such as pelvic pain, pelvic inflammatory disease as well as vulvar pain due to vulvitis, vulvar vestibulitis and so on.

• 한국환경과학회지
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• 제28권7호
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• pp.639-643
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• 2019

We aimed to evaluate antithrombotic efficacy of Protaetia brevitarsis extract during 21 days. Rats (SPF rat, weight 240~260 g) were divided into 16 groups (5 rats per group), they were: control group and Protaetia brevitarsis extract groups with dose of 0.1, 0.5, 1, 2.5, 5, 10, 25, 50, 75, 100, 200, 250, 500, 750, 1,000 mg/kg kg of body weight. Thromboplastin time (PT) and activated partial thromboplastin time (aPPT) as antithrombotic efficacy were tested in this animal experiment (at 7, 14 and 21 days). Overall, the admistration dose of Protaetia brevitarsis extract over 50 mg/kg at 7, 14 and 21 days for PT and over 25 mg/kg at 7, 14 and 21 days for aPPT tented to be longer than that of other groups. In addition, the optimal admistration doses of Protaetia brevitarsis extract to improves antithrombotic efficacy were 75, 100, 200 and 250 mg/kg at 7, 14 and 21 days for PT (p<0.05) and 50 and 100 mg/kg at 7 days, 75 mg/kg at 14 days, or 50, 100, 200 and 250 mg/kg at 21 days for aPPT (p<0.05). It can be concluded that Protaetia brevitarsis extract at optimal levels have antithrombotic efficacy.

• 한국미생물·생명공학회지
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• 제47권1호
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• pp.20-24
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• 2019

열매, 잎, 꽃, 봉우리, 꼬투리 등의 지상부를 약용 및 식용으로 사용하고 있는 모링가는 miracle tree라고 불리고 있다. 그러나, 현재까지 주로 잎과 씨에 대한 연구가 진행된 바, 지하부에 대한 성분 및 효능 연구는 초보적인 상태이다. 본 연구에서는 모링가 지하부의 유용 생리활성을 평가하기 위해 지하부의 ethanol 추출물 및 이의 순차적 유기 용매 분획물을 조제하고 이의 혈액응고 저해 및 혈소판 응집저해 활성을 평가하였다. 그 결과, 모링가 지하부 ethanol 추출물은 항응고 활성이 미약하고, 혈소판 응집 촉진효과를 나타내어 지혈작용을 나타내었으나, 추출물의 hexane 및 ethylacetate 분획에서는 우수한 트롬빈, 프로트롬빈, 혈액응고인자 저해와 함께 혈소판 응집저해 활성을 나타내었다. 또한 모링가 지하부 추출물 및 분획물들은 1.0 mg/ml 농도까지 인간 적혈구 용혈활성이 없음을 확인하여, 상기 분획물들이 신규의 항혈전제로 사용 가능함을 제시하였다.

• 대한본초학회지
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• 제28권3호
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• pp.39-44
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• 2013

Objectives : The purpose of this study was to identify active fraction and components from antiplatelet Ulmi cortex extract. Methods : The 70% ethanol extract of Ulmi cortex was subjected to column chromatography over D101 resin and eluted with an 20% (W1), 30% (W2), 40% (W3), 50%(W4), 70% (W5), and 100% ethanol (W6) to yield 6 fractions. W6 was further fractioned and its active components were purified using semi-preparative HPLC. The isolated compounds were identified by MS and NMR, and their contents were simultaneously analyzed using HPLC/UV. Antiplatelet aggregation activities of the fractions and the compounds were evaluated using rat platelet-rich plasma in presence of collagen ($5{\mu}g/ml$), arachidonic acid (0.05 U/ml), or thrombin ($100{\mu}M$). Results : Among six fractions, W3 prominently inhibited platelet aggregation. At the concentration of $200{\mu}g/ml$, W3 strongly inhibited arachidonic acid- and collagen-induced platelet aggregations by 78.2% and 65.9%, respectivley, and weakly inhibited thrombin-inducded platelet aggregation by 32.6%. Catechin, epicatehin, and catechin-7-O-${\beta}$-D-glucopyranoside were isolated from W3 and their contents were revealed to be 15.1%, 0.87%, and 0.32%. Catechin and epicatechin at the concentrations of $100{\mu}M$ strongly inhibited collagen-induced platelet aggregation by 79.9% and 86.6%, respectively, but weakly inhibited arachidonic acid- and thrombin-induced platelet aggregations. Conclusions : A main active principle of anitplatelet Ulmi Cortex extract is W3 fraction, of which main active component is catechin considering its antiplatelet activity and content.