• Journal of Life Science
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• v.19 no.7
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• pp.928-933
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• 2009

In an effort to discover an antiviral substance against noroviruse (NV), which causes gastroenteritis illness world-wide, several plants including spices and herbs were evaluated for their antiviral activities against feline calicivirus (FCV) as a surrogate for NV. Among them, methanolic extract of green tea (Camellia sinensis L.) exhibited significant antiviral activity against FCV. After treatment with green tea extract (3.13 mg/ml) for 1 hr, FCV was completely inactivated. The antiviral activity of green tea extract against FCV was also determined to be dose and time- dependent. The results obtained in this study suggested that green tea will be effective in the prevention of food-borne diseases caused by NV.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2020.08a
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• pp.50-50
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• 2020

본 연구는 천궁에 감염된 오이모자이크바이러스(CMV)와 천궁엽맥황화바이러스(CnVYV)의 제거를 위해 항바이러스제인 리바비린 처리 효과를 조사하기 위해 수행되었다. 천궁 기내식물체의 생장점을 절취 후 항바이러스제 농도를 달리한 배지에서 생육을 유도한 후 RT-PCR 방법을 통해 바이러스 검출율을 조사하였다. 리바비린을 40 mg l^-1 농도로 처리하였을 때 바이러스 검출 종 수는 1.1종으로 감소하였다. 또한 동일조건에서 CMV와 CnVYV-2의 검출율은 무처리구 대비 각각 1/5과 1/2 수준으로 감소하였다. 하지만 리바비린 처리에 따른 CnVYV-1의 검출율은 감소하지 않았다. 리바바린 처리 농도에 따른 생존율은 처리구별로 유의적인 차이가 나타나지 않았으나, 40 mg l^-1 리바비린 처리구에서 신초 발생 수와 신초 길이는 무처리구 대비 절반 수준으로 감소하였다. 리바비린 처리를 통해 CMV와 CnVYV-2가 미검출된 23개체를 선발하였으며, 추가적인 바이러스 검정 단계를 거쳐 무병묘 생산에 활용될 것이다.

• Korean Journal Plant Pathology
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• v.12 no.4
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• pp.432-436
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• 1996

역전사 중합효소 연쇄반응(RT-PCR)을 이용하여 담배(Nicotiana glutinosa)에 증식시킨 7종의 오이 모자이크 바이러스(CMV)를 검정하였다. RT-PCR을 위한 간단하고 신속한 바이러스 핵산의 조즙액 추출법이 개발되었으며, CMV 외피단백질 유전자 부위를 기초로 하여 제작한 20개의 염기로 구성된 primer를 사용하여 RT-PCR을 실시한 결과, 약 490 염기쌍의 DNA 단편들이 이병식물의 조즙액으로부터 증폭되었다. EcoRI 및 MspI을 이용한 RT-PCR 산물의 분석에 의하여, 공시한 7종의 바이러스는 모두 CMV subgroup I으로 동정되었다. Ouchterlony 한천젤 이중 확산법을 이용한 항혈청 검정에서도 7종의 바이러스 모두 CMV-Y의 항혈청과 단일의 침강선을 형성하였다.

• Proceedings of the Botanical Society of Korea Conference
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• 1994.07a
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• pp.93-110
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• 1994

• Journal of Life Science
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• v.32 no.5
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• pp.375-390
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• 2022

Influenza viruses are zoonotic respiratory pathogens, and influenza infections have caused a substantial burden on public health systems and the livestock industry. Although currently approved seasonal influenza vaccines have shown potent protection efficacy against antigenically well-matched strains, there are considerable unmet needs for the efficient control of viral infections. Enormous efforts have been made to develop broadly protective universal influenza vaccines to tackle the huge levels of genetic diversity and variability of influenza viruses. In addition, antiviral drugs have been considered important interventions for the treatment of viral infections. The viral neuraminidase inhibitor oseltamivir is the most widely used antiviral medication to treat influenza A and influenza B viruses. However, unsatisfactory clinical outcomes resulting from side effects and the emergence of resistant variants have led to greater attention being paid to plants as a natural resource for anti-influenza drugs. In particular, the recent COVID-19 pandemic has underpinned the need for safe and effective antiviral drugs with a broad spectrum of antiviral activity to prevent the rapid spread of viruses among humans. This review outlines the results of the antiviral activities of various natural products isolated from plants against influenza viruses. Special focus is paid to the virucidal effects and the immune-enhancing effects of antiviral natural products, since the products have broad applications as inactivating agents for the preparation of inactivated vaccines and vaccine adjuvants.

• The Korean Journal of Pesticide Science
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• v.14 no.3
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• pp.280-283
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• 2010

Extracts of 33 medicinal herbs belonging to 28 different families used as precious resources in the Northeast Asia were tested for their antiviral activities against two major plant viruses. Twenty one methanol extracts from 19 different families were found to have a antiviral activity against tobacco mosaic virus (TMV) and cucumber mosaic virus (CMV) when tested on indicator plants under greenhouse conditions. Most of these extracts have weak activities at the concentration used. But the methanol extracts of Phellinus linteus exhibited potent ($98.7{\pm}1.3%$) antiviral activity against TMV infection and also showed $97.2{\pm}2.2%$ activity against CMV infection. The methanol extracts of the gall of Rhus javanica also showed strong inhibitory efficacy over $98.7{\pm}1.1%$ against TMV or CMV infection. Further research is needed to elucidate the active constituents of these medicinal herbs which may be useful in the development of new and effective antiviral agent against plant viruses.

• The Science & Technology
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• v.28 no.12 s.319
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• pp.86-87
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• 1995

주류 메이커 (주)진로의 부설연구소인 진로종합연구소는 최근 항바이러스성 단백질을 이용한 새로운 항바이러스 생물농약제제를 개발해 업계의 관심을 끌고 있다. 농작물에 치명적인 피해를 주는 식물성 바이러스병에 대한 농약이 전무한 상태에서 이러한 무공해 생물농약을 개발한 것은 생명공학분야에서도 획기적인 일이며, 이밖에도 서울대 연구팀과 항AIDS 제제 등을 공동으로 연구하고 있어 결과가 주목된다.

• Research in Plant Disease
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• v.8 no.2
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• pp.92-100
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• 2002

For the survey of viruses infected in peanut cultivated in Korea, peanut seeds and leaves showing viral symptoms were collected from their growing areas. Typical symptoms on virus infected peanut leaves including mosaic, mottle with necrosis, yellowing, stripe or vein banding and stunts were observed. Two viruses isolated from the naturally infected peanuts were identified as Bean common mosaic virus(BCMV-PSt) and Peanut mottle virus(PeMoV) by their host range, immunosorbent elcetron microscopy(ISEM), direct immuno staining assay(DISA), RT-PCR, and intracellural symptoms. Direct negative staining method by electron microscope showed filamentous particles of about 780 m in length as well as inclusion bodies. In ultrathin sections of BCMV-PSt and PeMoV infected tissues, cytoplasmic cylindrical inclusions as well as filamentous virus particles were observed in the cytoplasm of parenchyma cells. ISEM revealed filamentous particles strongly decorated with antiserums of BCMV-PSt and PeMoV Peanut seeds were stained with BCMV-PSt and PeMoV antisera indicating the possibility of seed transmission far these viruses. Seedlings germinated from peanut seeds which reacted with antiserums of BCMV-PSt by DISA showed mild mottle or stripe symptoms while mosaic and necrotic mottle symptoms were observed for PeMoV-positive seedlings. Filamentous particles were strongly decorated with each antiserum under ISEM observation. BCMV-PSt coat protein gene of about 1.2 Kbp was amplified by RT-PCR. Altogether these results indicate that BCMV-PSt is the most prevalent virus infecting peanut in Korea.

• The Bimonthly Magazine for Agrochemicals and Plant Protection
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• v.7 no.5
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• pp.71-78
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• 1986

• Korean Journal of Medicinal Crop Science
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• v.11 no.1
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• pp.24-30
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• 2003

Aqueous extracts from eleven species of Korean medicinal plants were screened for antiviral activity against transmissble gastroenteritis virus (TGEV) and porcine epidemic diarrhae virus (PEDV). Seven of eleven plant extracts were found to have cytotoxicity for ST cells at the concentration of 1mg/ml, and ten extracts except Ephedra sinica were found to have non-cytotoxicity for Vero cells at the same concentration, Extracts of Zanthoxylum piperium, Cudrina tricuspidata, Clerodendron trichomum, Sophora flavescens, effectively inhibited TGEV, respectively. There were the antiviral activity against TGEV in the extracts of Glesdistsia sinensis, Euonymus alatus, Ficus carica, Solanum nigrum, Ephedra sinica, Xanthium strumarium, Acanthopanax seeliflorus in spite of their cytotoxicities. Nine extracts except Xanthium strumarium and Acanthopanax seeliflorus were found to have the antiviral activitity against PEDV. Extracts of Zanthoxylum piperium, Sophora flavescens was shown to have antiviral activities against both TGEV and PEDV.