• Title/Summary/Keyword: 항균율

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Antibacterial and Antibiofilm Activities of Alnus japonica Stem Extract against Porphyromonas gingivalis (Porphyromonas gingivalis에 대한 오리나무 줄기 추출물의 항균활성 및 생물막 형성 억제 효과)

  • Kim, Hye Soo;Cho, Soo Jeong
    • Journal of Life Science
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    • v.29 no.12
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    • pp.1386-1392
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    • 2019
  • This study investigated the potential of dye plants as natural oral health products. The antibacterial activity of ethanol stem extracts of A. japonica, R. verniciflua Stokes, G. jasminoides, D. morbifera, P. amurense Rupr., and S. japonica against P. gingivalis KCTC 5352, S. mutans KCTC3065, S. downei KCTC3634, S. sanguinis KCTC3284, and S. gordonii KCTC 3286 was confirmed. Among the stem extracts from 6 dye plants grown in Korea, ethanol extract from A. japonica stem (1 mg/disc) showed the highest antibacterial activity against P. gingivalis KCTC5352. The A. japonica stem extracts showed antibacterial activity similar to chlorhexidine, which was used as a positive control. The MIC and MBC of P. gingivalis KCTC5352 were 0.4 mg/ml and 0.6 mg/ml, respectively. The biofilm production rate and cell growth of P. gingivalis KCTC5352 in the cultures treated with 0.2-2.0 mg/ml of A. japonica extract were significantly decreased in a concentration-dependent manner. In addition, the mRNA expression of the superoxide dismutase and fimA associated with fimbriae formation in these cultures was suppressed, also in a concentration-dependent manner. Based on these results, it is concluded that A. japonica stem extracts can be used as an oral health product derived from natural materials, as demonstrated by its antibacterial action against and inhibition of biofilm formation of P. gingivalis KCTC5352.

Modified Atmosphere Packaging of Fresh Strawberries by Antimicrobial Plastic Films (항균성 플라스틱 필름을 이용한 딸기의 환경기체조절포장)

  • Chung, Sun-Kyung;Cho, Sung-Hwan;Lee, Dong-Sun
    • Korean Journal of Food Science and Technology
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    • v.30 no.5
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    • pp.1140-1145
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    • 1998
  • Low density polyethylene (LDPE) films incorporated with 1% antimicrobial agents of Rheum palmatum extract, Coptis chinensis extract, and Ag-substituted inorganic zirconium matrix, were applied to modified atmosphere packaging of 200 g fresh strawberries. Plain LDPE film package, PVC wrap and perforative pinhole package of the film impregnated with 1% Rheum palmatum extract were also constructed for comparative purpose. All the packages were stored for 13 days at $5^{\circ}C$ and measured in package atmosphere, microbial count and quality attributes of the strawberry fruits. The antimicrobial LDPE films retarded the growth in total aerobic bacteria, lactic acid bacteria and yeast on the fruits, and resulted in significantly lower decay. The degrees of reduced microbial growth and fruit decay in the antimicrobial film packages were more pronounced, when applied by hermetical sealing to produce the modified atmospheres of low $O_2$ (<4.0%) and $CO_2$ concentrations with $6.3{\sim}9.0%$. The hermetically sealed packages of antimicrobial LDPE films also showed better retention of fruit firmness and did not give any negative effect on the physical and chemical qualities of strawberries.

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Antihypertensive, Antimicrobial and Antifungal Activities of Buckwheat Hydrolysate (메밀 가수분해물의 항고혈압, 항균 및 항곰팡이 활성)

  • Do, Jeong-Ryong;Heo, In-Sook;Back, Su-Yeon;Yoon, Hye-Sook;Jo, Jin-Ho;Kim, Young-Myoung;Kim, Ki-Ju;Kim, Sang-Kyo
    • Korean Journal of Food Science and Technology
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    • v.38 no.2
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    • pp.268-272
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    • 2006
  • Antibacterial, antifungal, and Angiotensin-I-converting enzyme (ACE) inhibitory activities of buckwheat (Fagopyrum esculentum and F. tataricum) hydrolyzed by Viscozyme L and Alcalase 2.4 L were investigated. The Alcalase 2.4L-hydrolyzed buckwheat showed highest yield of 22.10-24.65%. F. esculentum hydrolysate treated with Viscozyme L from Salmonella typhimurium (clear zone: 3-4.7 mm) and Listeria monocytogenes (clear zone: 4-7.2 mm) showed highest antimicrobial activity among enzymes used. F. esculentum hydrolysate treated with Trichoderma reesei showed strongest antifungal activity among enzymes used (clear zone: 3.7-12 mm). Alcalase 2.4L-hydrolyzed F. esculentum and F. tataricum showed strong ACE inhibitory activities (61.19% and 94.48%, respectively).

The study of the safety inside skin for more efficient deodorization in safety helmets (안전모내 탈취효과 증대를 위한 내피의 안전성에 관한 연구)

  • Choi, Myeong-Ho;Ji, Yong-Hyeon;Gwak, Sang-Yeong;Lee, Jae-Yeong;Hwang, Sang-Tae
    • Proceedings of the KIEE Conference
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    • 2008.07a
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    • pp.2104-2105
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    • 2008
  • 작업자들이 현장에서 착용하는 안전모는 안전모 본연의 기능 때문에 통풍의 제한을 받게 된다. 이로 인해 작업자들은 작업 시 많은 땀을 흘리게 되며 작업자들이 흘리는 땀은 머리받침 끈과 머리고정대등에 흡수되어 각종세균을 번식하게 되고 이로 인해 냄새가 나게 된다. 최근 들어 원적외선 방사율이 뛰어난 천연소재들이 개발되고 있으며 원적외선은 항균과 탈취능력이 탁월한 것으로 알려지고 있다. 본 논문에서는 상기 나열된 문제점을 해소하기 위하여 상온에서 원적외선 방사율이 높은 소재와 수분의 흡,배출 능력이 뛰어난 소재를 사용하여 안전모내부의 냄새와 세균번식을 줄이는 방안을 제시 하고자 한다.

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Preparations and characteristics of the ceramic balls for heavy metals absorption and antibacterial activities in the drinking water (음용수중의 중금속흡착과 항균성용 세라믹 볼의 제조 및 특성평가)

  • Park, Chun-Won;Park, Ra-Young;Park, Sun-Min
    • Journal of the Korean Crystal Growth and Crystal Technology
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    • v.15 no.6
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    • pp.263-268
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    • 2005
  • The ceramic balls impregnated with $20{\sim}40nm$ sized Ag colloid were examined for heavy metals absorption and antibacterial activities in the drinking water. The preparation conditions of ceramic ball that the porosity was excellent were as follows: starting material: 85 wt% $Ca_{10}(PO_4)_6(OH)_2$, binder: 5 wt% PVA and 15 wt% ${\alpha}-Ca_3(PO_4)_2$, heating temperature: $1000^{\circ}C$, duration: 3 hrs. The ceramic balls obtained under these conditions showed specific surface area of $110m^2/g$, pore size of $120{\mu}m$ and porosity of 80%. Also, as the results of a performance test on a rate of adsorbing and removing heavy metals in the drinking water by using the.AAS, heavy metals such as Zn, Mn, Fe and Cu were removed to the extent that their content became 0.03mg/l or lower after 1 day and they showed an excellent bactericidal activity that all coliforms were killed after 3 hrs.

Effect of Enrofloxacin on Post Weaning Diarrhea in Pig (돼지의 이유자돈 설사증에 대한 Enrofloxacin의 효과)

  • Yeh Jae-Gil;Seo Jeong-Ki;Kim Byeung-Gie;Lee Chang-Koo;Han Byung-Woo
    • Journal of Veterinary Clinics
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    • v.8 no.1
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    • pp.59-63
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    • 1991
  • For the purpose of effective therapy and prevention of post weaning E. coli diarrhea, Enrofloxacin-(Baytril premix) was administered in the feed and a series of investigations were made the occurence of diarrhea, weight gain, feed conversion rate, changes In the counts of faecal E. coli and susceptibility of the isolated E. coli strains to antimicrobials. The results obtained were as follows 1. The weaned piglets which were treated with Baytril premix gained the average body weight of 3.2kg over the control group at the age of 62 days. The result indicates a marked weight gain effect which, by statiscal analysis, showed a significant difference(P<0.01). Administration of Baytril premix also resulted in the improvement of feed conversion rate and provention of post weaning diarrhea. 2. The counts of faecal E. coli in Baytri premx treated piglets showed a marked decrease from 3.14${\times}$10$\^$8/cells/g to 2${\times}$10$\^$7/cells/g at 5days post treatment and 1${\times}$10$\^$5/cells/g at 10 days post treatment. 3. 10 strains of E. coli isolated fred the experimental pig farm were highly susceptible to Bayoil, gentamicin and trimethoprim plus sulfamethoxazole.

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Partial Purification of Bacteriocin Produced by Enterococcus faecium MJ-14 Isolated from Meju (메주에서 분리된 Enterococcus faecium MJ-14가 생산하는 박테리오신의 부분정제)

  • Lee Jong-Gab;Lee Goon-Ja;Lim Sung-Mee
    • Journal of Food Hygiene and Safety
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    • v.20 no.4
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    • pp.211-216
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    • 2005
  • The bacteriocin produced by E. faecium MJ-14 was precipitated with $50\%$ saturated ammonium sulfate in MRS broth and then the precipitated protein was dissolved in 20 mM sodium phosphate buffer (pH 6.0). The crude bacteriocin was purified by CM-sepharose CL 6B and Sephacry S-100 column chormatograhy. In this case, the purification fold of the bacteriocin was 114, therefore, its activity was 127,293 BU/mg of specific activity. Result from SDS-PAGE of the purified bacteriocin, it was obtained two protein bands of 4.3 kDa and 5.8 kDa having antilisterial activity.

Study of UV Protection, Deodorization and Antimicrobial Properties of Cotton Fabrics Dyed with the Liquids Extracted from Salvia Plebia R. Br. (곰보배추 추출물로 염색한 면직물의 자외선 차단율, 소취성 및 항균성)

  • Jang, Hyun-Joo;Jung, Jin-Soun
    • Fashion & Textile Research Journal
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    • v.18 no.3
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    • pp.380-386
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    • 2016
  • This study examined the stainability and functionality of cotton dyed with the extract of Salvia Plebia R. Br. as a research preceding the development of health functional materials and fashion products with a healing motif. The CIELAB measurements of the cotton fabric dyed with the extract of Salvia Plebia R. Br. showed 73.32 for $L^*$, 1.7 for $a^*$, and 37.78 for $b^*$, while the Munsell measurements exhibited 2.63Y in color, 7.18 of brightness, and 5.49 of chroma. The degree of staining resulting from color fastness to laundering, and that resulting from color fastness to perspiration (acid and alkaline), as well as the level of color fastness to crocking (wet and dry) all stayed between relatively higher levels of 4 and 5. The fabric dyed also exhibited SPF 50+, an outstanding sun blocking performance. The deodorization rate was also excellent, rising from 97% to over 99% when the time elapsed increased from 30 minutes to 120 minutes. The fabric dyed also showed a 99.6% antimicrobial activity against staphylococcus aureus ATCC 6538, and a 71.4% antimicrobial activity against Klebsiella pneumoniae ATCC 4352. The aforementioned findings indicate that fabrics dyed with the extract of Salvia Plebia R. Br. contain the potential to be developed for use as health-related materials and fashion products that promote healing.

Study on the Functionality and Application of Natural-polymer with Biocompatibility (생체적합성을 가진 천연고분자의 응용 및 기능성에 관한 연구)

  • Kim, Tae-Hun;Kim, Doo-Eon;Sung, A-Young
    • Journal of the Korean Chemical Society
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    • v.53 no.5
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    • pp.547-552
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    • 2009
  • Chitosan, a natural polymer, has been importantly considered as biomedical materials due to its good biocompatibility and various bio-active characteristes. Water soluble chitosan was then copolymerized EGDMA(ethylene glycol dimethacrylate; used as a cross-linking agent for the free-radical copolymerization), MMA (methylmethacrylate), MA (methacrylic acid) in the presence of AIBN (azobisisobutyronitrile) as a radical initiator. The water content and visible transmissibility, ultimate strength of copolymerized ophthalmic polymer were measured to be 24$\sim$59%, 88$\sim$89% and 0.1$\sim$2.4 Kgf, respectively. And also, we tested for antimicrobial activities against staphylococcus aureus, Pseudomonas aeruginosa. They showed that in case of antimicrobial activities, the values including chitosan were much higher than that of the polymers of no including chitosan, suggesting that the copolymer can be used as a novel ophthalmic material of high performance.

Anti-biofouling properties of silver nano-particle coated artificial light-weight aggregates (은 나노 입자가 코팅된 인공경량골재의 생물오손 방지 특성)

  • Kim, Seongyeol;Kim, Yooteak;Park, Yongjoon
    • Journal of the Korean Crystal Growth and Crystal Technology
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    • v.25 no.5
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    • pp.212-217
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    • 2015
  • Ships and marine structures have a lot of problems in their high maintenance and operating cost by biofouling. A biofouling occurrs by the adhesion of marine microorganism, algae and bacteria. In this study, the aim is to prevent or to reduce the biofouling phenomena through silver nano-particle coating on artificial light-weight aggregates and geopolymer. The antibacterial activity on them is tested according to ASTM E2149-2013a. The test results showed, it is estimated that silver nano-particles removed 99.99 % of bacteria. Specimens were set up in the sea side of field test area in Korea Institute of Ocean Science and Technology (KIOST) and have been observed for five months. The anti-biofouling effect and difference in weight change rate have been detected two months later after the installation. Because silver nanoparticles inhibit bacterial growth and kill the cells by destroying bacterial membranes, silver nano-particle coating on artificial lightweight aggregates is a well-suited and eco-friendly method for preventing biofouling in the sea up to 5 months.