• Microbiology and Biotechnology Letters
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• v.37 no.2
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• pp.118-124
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• 2009

Esterase EM2L8 gene isolated from deep sea sediment was expressed in Escherichia coli BL21 (DE3) and the esterase activity of the cell-free extract was assayed using p-nitrophenyl butyrate-spectrophotometric method. Its optimum temperature was $40-45^{\circ}C$ and 45% activity of the maximum activity was retained at $15^{\circ}C$. The activation energy at $15-45^{\circ}C$ was calculated to be 4.9 kcal/mol showing that esterase EM2L8 was a typical cold-adapted enzyme. Enzyme activity was maintained for 6 h and 4 weeks at $30^{\circ}C$ and $4^{\circ}C$, respectively. When each ethanol, methanol, and acetone was added to the reaction mixture to 15% concentration, enzyme activity was maintained. In the case of DMSO, enzyme activity was kept up to 40% concentration. (S)-4-Chloro-3-hydroxy butyric acid is a chiral intermediate for the synthesis of Atorvastatin, a hyperlipemia drug. When esterase EM2L8 (40 U) was added to buffer solution (1.2 mL, pH 9.0) containing ethyl-(R,S)-4-chloro-3-hydroxybutyrate (38 mM), it was hydrolyzed into 4-chloro-3-hydroxy butyric acid with a rate of $6.8\;{\mu}mole/h$. The enzyme hydrolyzed (S)-substrate more rapidly than (R)-substrate. When conversion yield was 80%, e.e.s value was 40%. When DMSO was added, hydrolysis rate increased to $10.4\;{\mu}mole/h$. The plots of conversion yield vs e.e.s in the presence or absence of DMSO were almost same, implying that the reaction enantioselectivity was not changed by the addition of DMSO. Taken together, esterase EM2L8 had high activity and stability at low temperatures as well as in various organic solvents/aqueous solutions. These properties suggested that it could be used as a biocatalyst in the synthesis of useful pharmaceuticals.

• Journal of Food Hygiene and Safety
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• v.22 no.3
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• pp.213-217
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• 2007

LPS induces the synthesis of several inflammatory cytokine, chemokine, NO and inflammation in the liver of rats. The purpose of this study was to investigate the preventive effects of Rubus coreanum Miquel (RCM) In lipid metabolism. RCM of 100 mg/kg concentration was intraperitoneally administered into rats at dose of 1.5ml/kg for 20 days. On the day 21, 1.5ml/kg of LPS was injected 4 hours before anesthetization. We examined the lipid-related functions by measuring the levels of triglyceride (TG), total cholesterol (TC), total lipid (TL), high-density lipoprotein cholesterol (HDL-C) in serum and malondialdehyde(MDA) in liver tissue. The results showed that LPS treatment increased the values of TG, TC, TL and MDA, decreasing that of HDL-C. But RCM pretreatment decreased the high values of TG, TC, TL and MDA to the low values and increased the low value of HDL-C to the high value. These results suggested that RCM could be used as the potential candidate for the lipid metabolism natural supplement.

• Food Science and Preservation
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• v.11 no.3
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• pp.373-382
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• 2004

The purposes of this study were to investigate effects of Bambusae Caulis in Liquamen(BCL) on antioxidant activities and inhibitory activities of HMG-CoA reductase of in vitro, and lipid metabolism in rats fed the high cholesterol diet in vivo. Sprague-Dawley male rats weighing l00$\pm$10 g were devided into five groups ; normal group(NOR), the high cholesterol diet administered group(1 $\%$ cholesterol and 0.25$\%$ sodium cholate)(CON), 5$\%$ BCL administered group (5BL), the high cholesterol diet and 5$\%$ BCL administered group (5BCB) and the high cholesterol diet and 10\$\%$ BCL administered group (10BCB), respectively. In antioxidative activities of BCL using Rancimat in vitro, 1.25 diulent and original solution were more excellent activities than the control group, and in inhibiting activities of HMG-CoA reductase, BCL was shown inhibitory effects compared with the control, in dose dependent manners, especially 57.9$\%$ in original solution and 36.0$\%$ in 1.25 diulent. The growth rate of the control group was higher than the normal group, wheras the group given 5$\%$ BCL and 10$\%$ BCL were gradually decreased, especially the most excellent effect in 10$\%$ BCL. Serum levels of total cholesterol, LDL-cholesterol, triglyceride and free cholesterol were significantly decreased, whereas levels of HDL-cholesterol and phospholipid were increased, but not significantly. BCL administered group was increased in HDL-cholesterol/total cholesterol ratio and lowered antherogenic index. The activities of AST in serum were rather lowered in the BCL administration group than the cholesterol diet group, but not in ALT and ALP. The hepatic contents of total cholesterol were lowered significantly than control group, but not in triglyceride. Therefore, it might be expected that BCL is believed to be a possible protective or curative effects for fatty livers and hyperlipidemia-induced by a hi~h cholesterol diet.

• Journal of the Korean Society of Food Science and Nutrition
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• v.31 no.5
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• pp.788-795
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• 2002

This study described the effect of levan (9-2,6-linked fructose polymer) feeding on serum lipids, adiposity and uncoupling protein (UCP) expression in growing rats. Levan was synthesized from sucrose using bacterial levansucrase. UCP is a mitochondrial protein that uncouples the respiratory chain from oxidative Phosphorylation and generates heat instead of ATP, thereby increase energy expenditure. We observed that 3% or 5% levan containing diet reduced serum triglyceride levels, visceral and peritoneal fat mass and induced the UCP expression in rats fed high fat diet in previous study. To determine whether the intake of low level of levan may have the hypolipidemic and anti-obesity effect, 4 wk old Sprague Dawley male rats were fed AIN-76A diet for 6 wk, and sub-sequently fed 1% or 2% levan solution for further 5 wk. Intake of 1% levan in liquid form reduced serum triglyceride and serum total cholesterol levels to 50% and 66% of control group, respectively. Although epididymal and peritoneal fat masses were not affected by levan feeding, visceral fat mass was lower in 1% levan group compared to control group. The expression of UCP2 mRNA in brown adipose tissue, skeletal muscle and hypothalamus and UCP3 mRNA in skeletal muscle were not changed by levan feeding, while the UCP2 mRNA in white adipose tissue was up-regulated by levan feeding. In conclusions, intake of low level of levan solution reduced serum triglyceride and total cholesterol, restrained the visceral fat accumulation and increased UCP expression in white adipose tissue in rats. This study suggests that hypolipidemic and anti-obesity effect of levan attributed to anti-lipogenesis and inefficeint energy utilization by up-regulation of UCPs.

• Journal of the Korean Society of Food Science and Nutrition
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• v.40 no.11
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• pp.1512-1517
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• 2011

Effects of cultivated area on antioxidant compounds and antioxidant activities of sorghum were determined. The cultivated areas were Miryang (plain area of interior), Wonju (mountainous territory), and Sinan (coastal area), and the cultivated varieties were Hwanggeumchal-susu (HGCS), Daepung-susu (DPS), Whinchal-susu (WCS), Tojong-susu (TJS), and Mae-susu (MS). The highest total polyphenol contents of methanolic extracts were 19.64 and 19.32 mg gallic acid equivalent (GE)/g in un-hulled MS and DPS on the cultivated Wonju. The highest total flavonoid content was 7.54 mg catechin equivalent (CE)/g in un-hulled DPS on cultivated Wonju. Generally, antioxidant compound contents of sorghum on cultivated Wonju were higher than those of Miryang and Sinan. Further, un-hulled HGCS, DPS, and MS were higher than un-hulled WCS and TJS. The DPPH and ABTS radical scavenging activities of the methanolic extracts of sorghum did not significantly change with the cultivated areas, whereas there were significant changes with cultivated varieties. The radical scavenging activities of un-hulled HGCS, DPS, and MS with red or dark brown seed coats were higher than those of un-hulled WCS and TJS with white seed coats.

• Journal of the Korean Society of Food Science and Nutrition
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• v.38 no.7
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• pp.846-851
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• 2009

This study was carried out to investigate the effects of Puerariae Flos extracts on antioxidative potential, free radical generation and the lipid levels in rats. Sprague-Dawley rats were divided into 2 diet groups: AIN-76 diet (control group) and modified AIN-76 diet (cholesterol 0.5%) with 0.5% Puerariae Flos extracts for 4 weeks. Body weight and feed efficiency ratios from both groups were not significantly different. Antioxidative potentials significantly increased in the group fed Puerariae Flos extracts compared to control group (p<0.05). However, there was no difference in free radical generation. The weight of organs, such as heart, kidney, liver, and spleen, in rats were not different in both groups. The ratio HDL cholesterol to total cholesterol in the Puerariae Flos group was significantly higher than in the control group, while the other serum lipid parameters (total cholesterol, HDL cholesterol, triglyceride, and phospholipids) were not different between the two groups. These results imply that supplementation of Puerariae Flos extracts may beneficially contribute to improve antioxidant potential and to decrease the lipid levels in the blood.

• Journal of the Korean Society of Food Science and Nutrition
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• v.43 no.11
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• pp.1785-1790
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• 2014

This study was carried out to compare the physicochemical characteristics and sensory qualities of Sikhye (Korean traditional rice beverage) prepared with foxtail millet, proso millet, and sorghum. The cultivated varieties were Setaria italica Beauv. cv. Samdamae (SDM) and Samdachal (SDC), Panicum miliaceum L. cv. Ibaekchal (IBC), Sorghum bicolor (L.) Moench cv. Nampungchal (NPC), Donganme (DAM), Oryza sativa cv. Ilpum (IP), and Baegokchal (BOC). The brix degrees of SDM, SDC, IBC, NPC, DAM, IP, and BOC Sikhye were 9.53, 8.63, 5.67, 7.57, 6.27, 12.50, and $12.27^{\circ}Bx$, respectively. There were no significant differences in pH (5.99~6.10) among the groups. The highest turbidity was 1.07 in DAM Sikhye. The L-value, a-value, and b-value were 30.85~41.11, -0.34~2.52, and 2.56~5.67, respectively. Total polyphenol contents of SDM, SDC, IBC, NPC, DAM, IP, and BOC Sikhye were 241.52, 213.69, 202.34, 258.25, 193.24, 160.81, and $170.31{\mu}g\;GAE/mL$, respectively. Total flavonoid contents of Sikhye were $19.21{\sim}54.30{\mu}g\;CE/mL$. The highest DPPH and ABTS radical scavenging activities were $16.85{\pm}0.39$ and $64.75{\pm}2.92mg\;TE/100mL$ in DAM Sikhye, respectively. Finally, the sensory evaluation results indicate that there were significant differences in appearance, aroma, and taste between the groups, and SDM Sikhye was similar with IP and BOC.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.57 no.4
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• pp.424-429
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• 2012

This study was carried out to investigate the influence of different planting time on the synthesis of isoflavone in black soybean, Three varieties used in this experiment were lpumgeomjeongkong, Cheongjakong and Heugcheongkong which had different ecotypes, repectively. Seeds were sown at different time, May 15th, May 30th and June 15th with planting density of $60{\times}15cm$. In order to analyze the content of isoflavone, we collected sample every 5 days from 30 days after flowering to harvest and analyzed them with UPLC. As sowing was delayed, the content of isoflavone increased in all of three varieties. The content of genistein was greater than daidzein and glycitein. Increase of Glycitein was not distinct from 55 days after flowering(DAF) and it was stable against temperature change during the seed developing period. Although the content of genistein in Ilpumgeomjeongkong from 50 to 55 DAF, in Cheongjakong from 40 to 55 DAF and in Heugcheongkong from 60 to 65 DAF was lower than the content of daidzein, it was higher than that of daidzein afterward. In the statistical analysis on the relationship between average temperature and the content of aglycone isoflavone at 5-day intervals from 30 DAF during the grain filling period, genistein in Ilpumgeomjeongkong showed meaningful correlation as y=-15.28x+407.9 ($R^2=0.505^*$), diadzein in Cheongjakong showed meaningful correlation as y=-6.188x-164.5($R^2=0.454^*$), and genistein showed significantly high correlation as y=-11.59x+297.6 ($R^2=0.545^{**}$). Taking all the above results into consideration, it was suggested that the regions suitable for high content of isoflavone in black soybean be the northern area of Gyeonggi-do and Gangwon-do, Chungcheongbuk-do and inland area of Gyeongsangbuk-do, where are relatively low average temperature from flowering stage($R_2$) during the grain filling period.

• Korean Journal of Food Science and Technology
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• v.42 no.3
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• pp.298-303
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• 2010

This study was carried out to compare the physicochemical characteristics of Korean traditional wine fermented from foxtail millet and nuruk at different mixing rates. The alcohol contents of fermented wine ranged from 12.70 to 13.38%. Two kinds of commercial nuruks, SH and BS nuruk, were used. The brix degrees of foxtail millet wine fermented by SH and BS nuruks were 21.6 and $22.4^{\circ}Bx$, respectively. The pH, total acidity, and turbidity of the wines fermented by SH and BS nuruks were 3.74 and 3.40, 1.40 and 1.51%, and 0.441 and 0.149, respectively. With an increase in the amount of foxtail millet, brix degree, pH, turbidity, b-value and L-value decreased, and total acidity and a-value increased. Total color difference (${\Delta}Eab$) parameter of the wine fermented by SH nuruk were 8.58, 22.59 and 22.55, while those by BS nuruk were 0.35, 4.08 and 7.16 in 30, 70 and 100% addition rates of foxtail millet, respectively. With an increase in the amount added of foxtail millet, glucose content decreased. The organic acids such as lactic acid and acetic acid were predominantly detected in the fermented wine. Finally, based on sensory evaluations, the wine fermented by BS nuruk showed the best overall quality at the 30% addition rate of foxtail millet.

• The Korean Journal of Food And Nutrition
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• v.30 no.2
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• pp.218-225
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• 2017

This study was carried out to compare the cooked and antioxidant characteristics with of cooked mixed grain rice with an addition rate (0, 5, 10, 15 and 20%) of proso millet and various cooking methods. Mixed grain rice with added proso millet was cooked by general and high pressure cooking methods with and without fermented alcohol. All pasting characteristics, except for breakdown viscosity, decreased with increasing the amounts of proso millet. Water binding capacity and swelling power significantly decreased when increasing the amounts of proso millet, however the water solubility index significantly increased. The palatability characteristics of rice with added proso millet showed similar results to rice without proso millet. Total polyphenol contents of rice with 20% proso millet and fermented alcohol added, cooked by general and high pressure cooking methods, were $184.33{\pm}2.69$ and $179.80{\pm}5.22{\mu}g\;GAE/g$, respectively. Total flavonoid contents increased as amounts of proso millet increased. DPPH radical scavenging activities with and without proso millet added were 4.20~7.50 and 2.97~5.19 mg TE/100 g, respectively. ABTS radical scavenging activities with and without proso millet added were 14.36~27.92 and 14.07~15.83 mg TE/100 g, respectively. In this study, cooked and antioxidant characteristics of cooked mixed grain rice with added proso millet were expected to be used as basic data for manufactured processed products.