• 한국벤처창업학회:학술대회논문집
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• 2017.04a
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• pp.45-45
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• 2017

본 연구에서는 고압산소치료 후 포유동물의 피부에서 Melanin 세포의 생성이 줄어들었음을 이미 보고된 바 있고, 치료 후 전층 피부이식 생존율을 높였고, 또한 인체의 피부 진피층 확인은 더 많은 임상데이터가 필요하나 고압산소치료 후 표피면의 변화는 빠른 시간에 확인 할 수 있다고 판단됨으로 이에 피부진단기의 기준면과의 평행(parallel polarization) 이미지로 피부표피의 변화를 확인 하고자 했다. 본 연구의 고압산소챔버를 활용한 피부표피에 대한 분석 및 실험 결과, 주름살 및 상처(Wrinkle & Scar) 분석에서 피실험자 남자1 3%, 남자2 2%, 여자1 5.9%, 여자2 2.3%로 피실험자 모두 감소 현상을 보여 피부 탄력도가 좋아 지는 효과를 보였다. 피부미백(S-Gray) 분석에서는 피실험자의 피부 표피면의 멜라닌 및 에리즈마 색소의 피부 톤이 남자1 1.1%, 남자2 2.3%, 여자1 4% 로 피실험자 4명 중 3명은 상승 효과를 얻었으나, 피실험자 다른 1명인 여자2는 2.3% 하향의 결과가 나타났다. 홍도(Erythema) 분석, 피실험자 정상인 부위인 D.BLUE/BLUE 값이 남자1 5.6%, 남자2 4.9%, 여자1 17.3%, 여자2 15.3% 증가 현상을 보였으며 남자와 여자의 효과 차이가 10% 이상으로 나타낸 것으로 보아 남자 보다는 여자가 우세한 것으로 판단되었으며, 비정상인 에리즈마 색소(민감도)의 비정상인 부위인 YELLOW/RED 컬러 값에서 남자1 5.2%, 남자2 5%, 여자1 9.2%, 여자2 4.5% 감소 하였음을 보였다. 이에 피실험자 모두 에리즈마인 민감성 산소치료에 따른 피부에 미치는 영향이 효과가 있는 것으로 판명되었다.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.22 no.1
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• pp.27-39
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• 1996

Recently, in vitro stratum curneum model was a useful tool to evaluate the skin condition such as tranespidermal water loss, skin hydration and etc.. In this study to evaluate the alteration of SC with aging in hairless mouse, we measured TEWL using in vitro SC model, SC lipid, and outer corneocytes area. Although the total SC lipid was rich about 30% in 8 week old mouse compared with that of 82 week old mouse, the TEWL values were higher in the former than that of the latter. The outer corneocytes area was 559 $\pm$ 65 $mu extrm{m}$2 in 8 week old mouse and 755 $\pm$ 56 ${\mu}{\textrm}{m}$2 in 82 week old mouse. So the barrier function was reinforced with aging. This result suggested that the reinforced barrier function is one of the defense systems against the outer enviornment and the decreased skin function with aging.

• Korean Journal of Ichthyology
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• v.19 no.3
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• pp.201-209
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• 2007

The fine structures and histochemical features on the integumentary system of the grass puffer, Takifugu niphobles were examined by means of the light and transmission electron microscopy. Integumentary surface of the grass puffer showed irregular folds in light microscope. The folds of the ventral region are more pronounced than those in the dorsal region. Integumentary system is composed of outer epidermal layer and inner dermal layer. The stratified epidermal layer consists of epithelia, mucous cells, club cells, granular cells and multivacuolar gland. Epithelial cells are classified into superficial, intermediated and basal cell, and free surface of superficial cell is covered with microridges. Glands of the epidermal layer are divided into unicellular and multicellular gland. Mucous cells of multicellular gland contains mucosal materials of neutral glycoprotein. Multivacuolar gland is composed of numerous vacuole cells of about $20{\mu}m$ in axial diameter. Vacuole cells contains a large central vacuole and are connected to another by many desmosomes. The mucous glands and multivacuolar glands are more abundant in ventral region than dorsal integument. The thickness of dermis is more three to five times than epidermis in ventral integument. The collagen fibers, fibrocytes, nerve cells, basal plate of spine and chromatophore are observed in the dermal layer of compact connective tissue.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.34 no.1
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• pp.1-8
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• 2008

Epidermis is one of the most dynamic organs in the human body. Multiple layers of keratinocytes in the epidermis continuously undergo proliferation, differentiation, and desquamation cycles, which is the bases of maintaining the epidermal homeostasis. Epidermal homeostasis eventually leads to establish and maintain permeability barrier homeostasis, the most important function of the epidermis. The permeability barrier is located in the stratum corneum. Tightly coordinated regulations are required for the sustained normal barrier function. Extensive studies have established that several nuclear hormone liposensors, including peroxisome proliferator-activated receptor a PPARa, PPARb/d, PPARg and LXRs are expressed in keratinocyte. Activation of PPARs and LXRs could provide a mechanism to coordinate the formation of the corneocytes and extracellular lipid membranes that constitute the stratum corneum. Topical application of PPAR/LXR ligands to murine skin results in the increased expression of keratinocyte differentiation-related proteins, such as involucrin, loricrin, profilaggrin, and trans-glutaminase 1, which would stimulate cornified envelope formation. In conclusion, topical application of ligands or activators of PPAR/LXR as an epidermotherapy would be a promising option to deal dry skin conditions such as atopy.

• Proceedings of the Korean Society of Fisheries Technology Conference
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• 2001.05a
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• pp.475-476
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• 2001

어류의 피부와 아가미는 물이라는 외부환경과 직접 접촉하고 있기 때문에, 삼투압의 불균형과 환경유해성분 등에 직면하게 되어 상피층의 이상증식을 유발한다(Hawkes, 1974). 청어류, Clupea harengus 자어시기에 철의 영향으로 인해 피부계 미토콘드리아가 위축되며, 활면소포체가 감소한다고 보고되었다 (Somasundaran, 1985) 해양생물에 있어서 유기오염원의 생체내 축적은 수용액의 상태로 아가미와 체표의 흡수 및 먹이의 섭취를 통해서 일어나며, 이 가운데 아가미를 통해서 흡수되는 경우가 높다. (중략)

• Journal of the Society of Cosmetic Scientists of Korea
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• v.44 no.1
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• pp.31-37
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• 2018

Oil/water (O/W) nanoemulsions are effective vehicles to change the permeability of the skin. In this study, we focused on the preparation and characterization of nanoemulsion which serve as colloidal carriers for the dermal application of ceramide IIIB (CIIIB) and stratum corneum (SC) lipids such as cholesterol, and palmitic acid. In order to optimize the nanoemulsions, emulsification process conditions were conducted with regard to droplet size, nanoemulsion stability, and solubility of CIIIB. A decrease in droplet size was observed through emulsification temperature of $80^{\circ}C$ and phase inversion composition (PIC) method. CIIIB has low solubility in oil and water. When the concentration of CIIIB was increased, the droplet size of nanoemulsion was increased. When Lipoid S75-3 was added to the oil phase, the solubility of CIIIB increased, indicating some interactions shown in DSC measurements. CIIIB and SC lipids could be successfully incorporated in nanoemulsions without crystallization or physical instability. In conclusion, a stable nanoemulsion containing the SC lipids could be effective as an efficient moisturizing system for skin.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2022.09a
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• pp.25-25
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• 2022

피부 노화는 피부와 피부 지지층 등의 광범위한 퇴행 과정을 말한다. 피부 노화의 원인은 흡연, 공해, 스트레스 등이 있지만, 그 중에서도 자외선(ultra violet, UV) 조사가 가장 큰 요인으로 꼽힌다. 반복적인 자외선 조사에 의해 진행되는 피부노화를 광노화라고 하며 그 가장 큰 특징으로는 콜라겐 섬유와 엘라스틴의 감소로 야기되는 주름을 들 수 있다. 본 연구에서는 제주에서 채집한 바위수국의 추출물 및 분획물의 항산화 및 자외선으로 인한 피부노화 예방(anti-photoaging) 효능을 확인하고, 활성물질을 분리하여 광노화 예방 효능과 그 메커니즘을 확인하였다. 실험에 사용된 바위수국은 범의귀과의 덩굴성 식물로 바위면이나 나무줄기 등에 붙어서 자라며, 한국(제주, 울릉도)과 일본에 분포한다. 바위수국 추출물과 분획물에서 총 페놀 함량. 총 플라보이드 함량, DPPH 및 ABTS 라디칼소거 활성의 항산화 실험 결과, 부탄올과 에틸아세테이트 분획층에서 강력한 항산화 활성이 관찰되었다. 또한 UVA를 조사한 인간 진피 섬유아세포 (human dermal fibroblast, HDF)데 대한 콜라겐 분해효소인 matrix metalloproteinase-1(MMP-1) 생성 억제 활성을 확인한 결과, 부탄올 분획층이 세포 생장 저해 없이 가장 우수한 효능이 확인되었다. 따라서 부탄올 분획층에서 주요 성분 분리 실험을 수행하여 총 4개의 화합물을 분리하였다; Chlorogenic acid (1), Quercetin-3-O-glucosyl-(1-2)-rhamnoside (2), Quercetin-3-O-xylosyl-(1-2)-rhamnoside (3), Quercitrin (4). 분리한 4개의 물질의 MMP-1 생성 억제 활성을 비교한 결과 화합물 2가 세포독성 없이 MMP-1 생성 억제 효능이 우수하였고, 이후 화합물 2의 광노화 예방 효능과 그 메커니즘을 확인하였다. 화합물 2는 MMP-1의 생성을 억제할 뿐만 아니라 procollagen type I의 생성을 증가시켰으며, MMP-1 생성에 관여하는 mitogen-activated protein kinase (MAPK)/activator protein-1 (AP-1) 신호전달경로를 하향 조절하며, 콜라겐 생성과 관련된 Transforming growth factor-β (TGF-β)/Smad 신호전달경로를 상향 조절하여 UVA에 의한 광노화 예방에 효능을 나타내었다. 이러한 결과들을 바탕으로, 바위수국은 항노화(anti-aging) 기능성 화장품 및 이너뷰티 기능성 식품 소재로 개발이 가능할 것으로 기대된다.

• Korean Journal of Ichthyology
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• v.19 no.2
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• pp.120-127
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• 2007

To investigate the skin of the flat-headed goby, Luciogobius guttatus, it was used 8 body regions such as the head, the upper and lower jaw, the chin, the back, lateral region, abdomen, and the operculum. The epidermis consisted of three layers: the outermost layer, middle layer and stratum germinativum (basal layer). The outermost layer consisted of rather flattened cells arranged in 1 to 4 layers and mucous cells. The middle layer consisted of large epidermal cells occupying the height of the epidermis with 1 to 10 layers, causing a web-shaped structure. Due to the large epidermal cells of the middle layer, L. guttaus had a thick epidermis. The large epidermal cell contains tonofilaments, lucent contents, and desmosome. The basal layer was comprised of a single layer having cuboidal cells. A large number of fine blood capillaries were found just under the basal layer. The dermis consisted mostly of stratum compactum with numerous blood capillaries but had no scale.

• Applied Microscopy
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• v.31 no.4
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• pp.325-331
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• 2001

Integumentary structures of the stone flounder, Karefus bicoloratus were examined by means of the light and transmission electron microscopy. Stratified epidermal layer consists of supporting cells, unicellular glands and granular cells. The epidermal layer could be classified into superficial, intermediated and basal layer by morphology and structure of the supporting cells . The cytoplasm of supporting cells is divided into cortex and medullar part. In the cortex microfilaments are well developed. Mucous cells of unicellular gland were observed in the superficial and intermediated layer of the epidermis. The mucous materials were identified as glycoprotein of neutral and carboxylated mucosubstance by histochemical methods. Club cell has well developed smooth endoplasmic reticula and Golgi complex in the cytoplasm. Granular cells were observed in the intermediated and basal layer, and the cytoplasm is occupied with membrane-bounded granules of electron dense. Three types of pigment cells could be distinguished with electron density of cytoplasmic inclusions. Nerve myelins were observed near the pigment cells.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.40 no.3
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• pp.259-268
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• 2014

Non-invasive technologies in skin research have enabled to use a live image of living skin without a biopsy or histologic processing of tissue. Confocal scanning laser microscope (CSLM) operated at a near-infrared wavelength of 830 nm allows visualization of inner structure of skin as a non-invasive manner. According to previous researches using CSLM, melanin cap and papillary ring were clearly observed in pigmented areas between stratum basale and papillary dermis. In this study, conversional analysis of CSLM digital images into numerical estimation using scanning probe image processor (SPIP) software was attempted for the first time. It is concluded that a quantification of CSLM images can pave way to expand the field of applications of CSLM.