• Journal of the Korean Society of Clothing and Textiles
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• v.45 no.2
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• pp.390-407
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• 2021

The study aims to assess the current status of sustainable clothing habits from the perspective of consumers. Awareness and management behavior regarding microplastics from fashion products and usage of fleece and faux fur were investigated. A random online survey involving 413 women was conducted to figure out their perceptions on microplastics that are shedded from fashion products such as fleece and faux fur. The results indicate that 73.6% were not aware of the fact that microplastic is released during the washing process of fleece and faux fur. Furthermore, only 26.6% of the respondents who were aware of microplastics from clothing washing were making efforts to reduce its emission. The respondents considered product sustainability more in the selection stage than in the management stage (p<.001). The results revealed that, although the respondents were highly aware of the risk of environmental pollution that microplastics pose, they were neither fully cognizant of the fact that microplastics may come from fashion products, nor did they make efforts to reduce its emissions. Compared with respondents in their 20's, respondents in the age of 30-40 years seemed more aware of microplastics from fashion products and exerted more effort to reduce its emission.

• Korean Journal of Microbiology
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• v.52 no.1
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• pp.110-115
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• 2016

A small plasmid (pDK4) from the Antarctic marine organism Pseudoalteromonas sp. PAMC 21150, was purified, sequenced and analyzed. pDK4 was determined to be 3,480 bp in length with a G+C content of 41.64% and contains three open reading frames encoding a replication initiation protein (RepA), a conjugative mobilization protein (Mob) and a hypothetical protein. PCR-amplified pDK4 was cloned in high-copy pUC19 to yield the fusion vector pDOC153. The chloramphenicol resistance gene was inserted into pDOC153 to give an ampicillin and chloramphenicol-resistant, Pseudoalteromonas - Escherichia coli shuttle vector (7,216 bp; pDOC155). The TonB-dependent receptor (chi22718_IV ) and exochitinase (chi22718_III ) genes from Arctic marine P. issachenkonii PAMC 22718 were cloned into pDOC155 to produce pDOC158 and pDOC165, respectively. Both vector derivatives were transferred into plasmid-free Pseudoalteromonas sp. PAMC 22137 by the triparental mating method. PCR experiments showed that the genes were stably maintained both in Pseudoalteromonas sp. PAMC 22137 and E. coli $DH5{\alpha}$ cells, indicating the potential use of pDOC155 as a new gene transfer system into marine Pseudoalteromonas spp.