• Research in Plant Disease
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• v.27 no.4
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• pp.172-179
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• 2021

This study aimed to assess the disease incidence and distribution of toxigenic in Korean triticale. The pathogen of triticale that cause Fusarium head blight were isolated from five different triticale cultivars that cultivated in Suwon Korea at 2021 year. The 72 candidate were classified as a Fusarium asiaticum by morphology analysis and by ITS1, TEF-1α gene sequence analysis. And the results of pathogenicity with 72 isolates on seedling triticale, 71 isolates were showed disease symptom. Also, seven out of 71 Fusarium isolates were inoculated on the wheat, to test the pathogenicity on the different host. The results showed more low pathogenicity on the wheat than triticale. The results of analysis of toxin type with 72 isolates, 64.6% isolates were produced nivalenol type toxin and other 4.6% and 30.8% isolates were produce 3-acetyldeoxynivalenol and 15-acetyldeoxynivalenol, respectively. To select fungicide for control, the 72 Fusarium isolates were cultivated on the media that containing four kinds fungicide. The captan, hexaconazole, and difenoconazole·propiconazole treated Fusarium isolates were not showed resistance response against each fungicide. However, six isolates out of 72 isolates, showed resistance response to fludioxonil. This study is first report that F. asiaticum causes Fusarium head blight disease of triticale in Korea.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.67 no.1
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• pp.41-52
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• 2022

As a cultivar of Korean wheat, 'Keumgang' wheat variety has a fast growth period and can be grown stably. Hexaploid wheat (Triticum aestivum) has moderately high salt tolerance compared to tetraploid wheat (Triticum turgidum L.). However, the molecular mechanisms related to salt tolerance of hexaploid wheat have not been elucidated yet. In this study, the candidate genes related to salt tolerance were identified by investigating the genes that are differently expressed in Keumgang variety and examining salt tolerant mutation '2020-s1340.'. A total of 85,771,537 reads were obtained after quality filtering using NextSeq 500 Illumina sequencing technology. A total of 23,634,438 reads were aligned with the NCBI Campala Lr22a pseudomolecule v5 reference genome (Triticum aestivum). A total of 282 differentially expressed genes (DEGs) were identified in the two Triticum aestivum materials. These DEGs have functions, including salt tolerance related traits such as 'wall-associated receptor kinase-like 8', 'cytochrome P450', '6-phosphofructokinase 2'. In addition, the identified DEGs were classified into three categories, including biological process, molecular function, cellular component using gene ontology analysis. These DEGs were enriched significantly for terms such as the 'copper ion transport', 'oxidation-reduction process', 'alternative oxidase activity'. These results, which were obtained using RNA-seq analysis, will improve our understanding of salt tolerance of wheat. Moreover, this study will be a useful resource for breeding wheat varieties with improved salt tolerance using molecular breeding technology.

• Journal of Environmental Science International
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• v.31 no.5
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• pp.413-422
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• 2022

To identify rice (Oryza sativa L.) cultivars suitable for Italian ryegrass (Lolium multiflorum Lam.)-rice double cropping systems, we investigated the yield and grain quality of four different midseason maturing rice cultivars ('Daebo', 'Haepum', 'Haiami', and 'Samdeog') and four midseason-to-late maturing rice cultivars ('Hyunpoom', 'Saeilmi', 'Saenuri', and 'Samkwang') in single rice cropping and Italian ryegrass-rice double cropping systems in paddy fields of Miryang, South Korea. We found that organic matter and available P₂O₅ content slightly decreased, whereas Na content increased, in the soil where Italian ryegrass was cultivated during winter compared to that in the soil that remained fallow during winter. The pH, electrical conductivity, organic matter, and contents of K⁺, Ca²⁺, Mg²⁺, and Na⁺ decreased, whereas the available P₂O₅ content slightly increased, in the soil where rice was harvested in both single and double cropping systems. However, compared to the optimum soil conditions for rice cultivation, available P₂O₅ and K⁺ content were high and Mg²⁺ content was low in both single and double cropping systems. At the heading stage, the culm length and leaf color slightly increased in most of the rice cultivars, whereas the panicle length and number slightly decreased, in the double cropped system. After harvesting, spikelet number and milled rice yield did not show a significant difference between single and double cropping systems. However, the ripened grain rate and weight per thousand grains increased slightly in the 'Saeilmi' and 'Samkwang' cultivars but remained either stable or slightly low in other cultivars in the double cropping system. The milled rice yield was high (> 500 kg/10a) in 'Daebo' and 'Haepum' among midseason maturing rice cultivars, and in 'Saeilmi' and 'Saenuri' among midseason-to-late maturing rice cultivars, in both single and double cropping systems. The head rice rate was high in midseason maturing rice cultivars in the double cropping systems, reaching > 70% in 'Haepum' and 'Haiami' cultivars, whereas it decreased in most midseason-to-late maturing rice cultivars (excluding 'Samkwang' cultivar), in double cropping systems. Particularly, it exceeded > 70% in the 'Saenuri' cultivar in both single and double cropping systems. The protein content in milled rice increased, whereas the amylose content either remained stable or slightly increased, in double cropping systems. The Toyo taste value decreased in all midseason-to-late maturing rice cultivars and slightly increased in the 'Daebo' and 'Haiami' cultivars among midseason maturing rice cultivars in double cropping systems. However, Toyo taste values in the 'Haepum', 'Haiami', and 'Saenuri' cultivars exceeding > 80% in both single and double cropping systems. Therefore, we recommend 'Haepum', 'Haiami', and 'Saenuri' cultivars as candidates for Italian ryegrass-rice double cropping systems due to high yield, head rice rate, and Toyo taste value.

• Journal of the Korean Society of International Agriculture
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• v.23 no.5
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• pp.546-551
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• 2011

Chrysanthemums, often called mums or chrysanths, belong to the genus Chrysanthemum, which includes about 30 species of perennial flowering plants in the family Asteraceae. We extracted DNA from Dendranthema grandiflorum ('Smileball') to construct a simple sequence repeat (SSR)-enriched library, using a modified biotin-streptavidin capture method. GS FLX (Genome Sequencer FLX System which provides the flexibility to perform the broad range of applications) sequencing (at the 1/8 run specification) resulted in 18.83 mega base pairs (Mbp) with an average read length of 280.06 bp. Sequence analyses of all SSR-containing clones revealed a predominance of di-nucleotide motifs (16,375, 61.5%) followed by tri-nucleotide motifs (6,616, 24.8%), tetra-nucleotide motifs (1,674, 6.3%), penta-nucleotide motifs (1,283, 4.8%), and hexa-nucleotide motifs (693, 2.6%). Among the di-nucleotide motifs, the AC/CA class was the most frequently identified (93.5% of all di-nucleotide types), followed by the GA/AG class (6.1%), the AT/TA class (0.4%), and the CG/GC class (0.03%). When we analyzed the distribution of different repeat motifs and their respective numbers of repeats, regardless of the motif class, of 100 SSR markers, we found a higher number of di-nucleotide motifs with 70 to 80 repeats; we also found two di-nucleotide motifs with 83 and 89 repeats, respectively, but their product lengths were within optimum size (297 and 300 bp). In future work, we will screen for polymorphisms of possible primer pairs. The results will provide a useful tool for assessing molecular diversity and investigating the population structure among and within Chrysanthemum species.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.68 no.4
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• pp.276-284
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• 2023

Nitrogen is a major and essential macronutrient for plant growth and development. However, excessive nitrogen application can lead to ecological pollution or greenhouse gas emissions, consequently resulting in climate change. In this study, we used 153 genetic resources of rice to evaluate the effects of the levels of nitrogen application on grain yield and yield-related traits. Significant differences were noted in the yield and yield-related traits of genetic resources between two nitrogen application levels, namely, 4.5 kg/10a (NN: normal nitrogen condition) and 9.0 kg/10a (LN: low-nitrogen condition). Among the tested traits, days to heading (DTH), clum length (CL), grain yield per plant (GYP), number of panicles per plant (NPP), and number of spikelets per panicle (NSP) decreased by 1.8 to 17.9% when the nitrogen application levels decreased from NN to LN. The 1,000-grain weight (TWG) and percentage of ripened grain (PRG) increased by 2.6 to 11.2% under these conditions. Based on nitrogen application levels, two-way analysis of variance (ANOVA) demonstrated significant differences in GYP, NPP, and PRG but not in NSP and TGW. NPP exhibited negative correlations with NSP (-0.44) and TGW (-0.44), and TGW displayed a negative correlation with PRG (-0.34), whereas, GYP exhibited a positive correlation with PRG (0.37) and NSP (0.38). A similar pattern was recorded under the LN condition. NPP, TGW, and PRG were clustered as PA (principle axis) 1 under the LN condition by factor analysis. NSP and GYP were clustered as PA (principle axis) 2. These results demonstrated NPP and NSP as the primary factors contributing to the decrease in grain yield under LN conditions. In conclusion, we selected eight genetic resources that exhibited higher GYP under both NN and LN conditions with higher NPP or NSP. These genetic resources can be considered valuable breeding materials for the adaptation of plants to nitrogen deficiency.