• Journal of The Korean Society of Grassland and Forage Science
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• v.33 no.2
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• pp.100-104
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• 2013

Zoysiagrass (Zoysia japonica Steud.) is an important forage and turfgrass that spreads by stolons and rhizomes. Zoysiagrass stolon can be used directly for Agrobacterium-mediated genetic transformation by exploiting the potential of direct shoot formation. However, surface sterilization of field-grown stolons is difficult and remains to be explored. We developed an effective surface sterilization and culture method using the stolon explant for infection with Agrobacterium tumefaciens. Among various treatments, sequential disinfection in 30% bleach for 15 min followed by 0.1% mercuric chloride for 25 min resulted in the highest number of clean stolons. The efficacy of mercuric chloride was increased under vacuum conditions by incubating at 800 mbar for 5 min. The inclusion of 2.5 mg/l amphotericin B further prevents fungal growth in in vitro cultures. This protocol would speed up the development of transgenic plants by utilizing field-grown stolon nodes.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.33 no.4
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• pp.420-428
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• 1988

Root is supposed to be important role in growth and yield formation of soybeans and also there is expected to exist varietal differences between soybean root morphology and system. 30 soybean varieties including 10 Korean recommended, 10 Introduced and 10 Korean native varieties were grown at field and pot under standard culture condition. Top and root characteristics were investigated at V4, R1 and R5 stage and T/R relations, correlations with yield were calculated. Top characteristics showed significant difference between 3 variety groups of Korean recommended, Introduced and Korean native variety. Stem length at R1 stage and stem length, stem diameter, number of nodes, number of branches, number of pods and grain yield at R5 stage were significantly different top characteristics. Root characteristics showed significant difference between 3 variety groups were number of thick primary root at V4 stage, primary root angle and root cutting strength at R1 stage, thick root diameter and root dry weight at R5 stage. The correlations between top and root characteristics and soybean yield was greatly different by soybean variety groups, and the top characteristics was more highly correlated then root characteristics. All the top and root characteristics of Korean recommended variety showed no correlations with yield. However, leaf area, stem length, top dry weight in Introduced variety and leaf area, top dry weight, number of nodes, number of thick primary root in Korean native variety were highly correlated with soybean yield.

• Journal of Food Hygiene and Safety
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• v.28 no.4
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• pp.376-380
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• 2013

Determination of PFOS in instant food packing materials by LC-MS/MS was developed. The procedure involves an extraction of the Food Packing Materials with 100% methanol soxcelet extraction method. The LC separation was performed by Hypersil Gold ($150mm{\times}2.1mm$ 5 um) with mobile phases of 2 mM amoniumacetate solution and acetonitrile. The Mass spectral acquisition was done in negative ESI/SRM using the TSQ Quantum Ultra. With this method, good linear relationship, sensitivity and reproducibility were obtained. The rate of recoveries of PFOS from paper material spiked with 1.0 ug/L were 99.84%, respectively. The limit of quantitation and limit of detection were below 0.03 ug/L and 0.009 ug/L. The method had been applied to determination of PFOS in instant food packing materials.

• Journal of the Society of Disaster Information
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• v.15 no.4
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• pp.459-468
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• 2019

Purpose: The cavity exploration of the lower part of the road is carried out to prevent ground-sinking. However, the detected communities cannot be identified by the cavity location and history information, such as repackaging the pavement. Therefore, the field applicability of RFID systems was evaluated in this study to enable anyone to accurately identify information. Method: During temporary recovery, tag recognition distance and recognition rate were measured according to underground burial materials and telecommunication tubes using RFID systems with electronic tag chips attached to the bottom of the rubber cap. Result: The perceived distance and perceived rate of depth for each position of the electron tag did not significantly affect the depth up to 15cm, but it did have some effect if the depth was 20cm. In addition, water effects from nearby underground facilities and rainfall are relatively small, and the effects of wind will need to be considered during the weather conditions of the road. Conclusion: The RFID tags for field application of the pavement management system store various information such as location and size of cavity, identification date, cause of occurrence, and surrounding underground facilities to maximize cavity management effect with a system that can be computerized and mobile utilization.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2019.10a
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• pp.53-53
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• 2019

방울다다기양배추는 아직까지 국내에서는 소비자들에게 인지도가 낮은 작물이지만 최근 식생활 및 소비 패턴이 변화하면서 편의성, 차별성 등이 중요시면서 미니 과일, 미니채소류에 대한 관심이 증가하고 있다. 또한 외국의 도입 채소류에 대한 관심이 증가하면서 2013년 방울다다기양배추 수입량이 36톤이었던 것이 2015년에는 14.3배가 증가한 515톤으로 증가하였다. 이를 통해 방울다다기양배추가 가지고 있는 기능성과 미니채소에 대한 관심으로 지속적으로 소비가 증가할것으로 예상되고 있다. 이와같이 증가추세에 있는 방울다다기양배추의 수입을 대체하기 위해서는 국내 재배면적 확대가 필요하나 저온성 작물인 방울다다기양배추는 고온기 남부지역에서는 재배가 어려워 제주지역의 겨울재배 작형으로 제한되어 재배가 되고 있다. 따라서 제주지역의 겨울재배 작형과 연계하여 강원도 고랭지의 서늘한 여름 기후를 이용하여 단경기인 9~11월에 생산할 수 있는 여름재배 작형을 개발할 필요성이 있어 시험을 수행하였다. 본 시험은 2018년~2019년 태백 철암의 노지(표고 750 m)에서 시험품종은 "브릴리언트"를 이용하여 3월 하순 파종하여 50일 육묘하여 5월 10일 포장에 정식하였다. 흑색멀칭한 시험포장에 $80{\times}30cm$부터 $80{\times}60cm$까지 10 cm 간격으로 차이를 두고 정식하였으며 정식 전 N-P-K = 20-15-20 kg/10 a를 시비하였고 시험포장은 수확까지 병해충 방제 등 주기적으로 관리하면서 생육특성 및 수량성을 조사하였다. 재식거리별 생육상황을 비교한 결과 초장은 처리간 큰 차이가 없었고 경경은 재식거리가 커질수록 증가하는 경향이었다. 또한 엽장 및 엽폭은 처리간의 경향이 없었으며 엽수는 재식거리가 커질수록 증가하였고 $80{\times}30cm$ 처리시 80매내외였던 것이 $80{\times}50cm$ 이상일 경우 100매 이상으로 증가하였다. 재식거리별 구의 특성을 조사한 결과 구경은 처리간 큰 차이는 없었으나 재식거리가 커질수록 구경이 다소 증가하였으며 구중도 증가하는 경향이었다. 방울다다기양배추의 구의 엽수는 처리간 큰 차이가 없었고 경도 및 당도도 큰 차이가 없었다. 처리별 수량은 $80{\times}40cm$와 $80{\times}50cm$ 처리에서 수량이 다소 증가하였고 상품화율은 $80{\times}50{\sim}60cm$ 처리에서 80%이상으로 높았다. 따라서 방울다다기양배추의 여름 고랭지 재배시 재식거리는 $80{\times}50cm$ 처리시 생육 및 엽수 증가에 따른 구의 형성이 증가하여 수량성이 높아지고 상품화율도 증가하였다.

• Clean Technology
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• v.24 no.4
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• pp.269-274
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• 2018

In this study, Life Cycle Assessment (LCA) of wet tissue manufacturing process was performed. The wet tissue manufacturing process consists of preparation of wetting agent (chemical liquid), impregnation of nonwoven fabric into wetting agent and primary and secondary packaging. Data and information were collected on the input and output of the actual process from a certain company and the database of the Korea Ministry of Environment and some foreign countries (when Korean unavailable) were employed to connect the upper and the lower process flow. Based on the above and the potential environmental impacts of the wet tissue manufacturing process were calculated. As a result of the characterization, Ozone Layer Depletion (OD) is 3.46.E-06 kg $CFC_{11}$, Acidification (AD) is 5.11.E-01 kg $SO_2$, Abiotic Resource Depletion (ARD) is $3.52.E+00\;1yr^{-1}$, Global Warming (GW) is 1.04.E+02 kg $CO_2$, Eutrophication (EUT) is 2.31.E-02 kg ${PO_4}^{3-}$, Photochemical Oxide Creation (POC) was 2.22.E-02 kg $C_2H_4$, Human Toxicity (HT) was 1.55.E+00 kg 1,4 DCB and Terrestrial Ecotoxicity (ET) was 5.82.E-04 kg 1,4 DCB. In order to reduce the environmental impact of the manufacturing process, it is necessary to improve the overall process as other general cases and change the raw materials including packaging materials with less environmental impact. Conclusively, the energy consumed in the manufacturing process has emerged as a major issue, and this needs to be reconsidered other options such as alternative energy. Therefore, it is recommended that a process system should be redesigned to improve energy efficiency and to change to an energy source with lower environmental impact. Due to the nature of LCA, the final results of this study can be varied to some extent depending on the type of LCI DB employed and may not represent of all wet tissue manufacturing processes in the current industry.

• Journal of the Korean Institute of Landscape Architecture
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• v.46 no.6
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• pp.120-126
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• 2018

The study conducted an experiment in which residual aggregate and polypropylene fibers are mixed in concrete, and an experiment in which granite and polypropylene fibers are mixed. Two types of experiments, in particular, changed the amount of polypropylene fibers, and examined the mechanical properties of slump, compressive strength, tensile strength and the like. To establish a light and easy-to-use material for landscape construction and packaging material development by comparing two kinds of experimental results, comparing and analyzing residual aggregate as experimental materials and materials using granite soil to prevent partial destruction due to cracks in drying shrinkage. The more the amount of the PP fibers increases in concrete, the more the volume of the PP fibers increases, the less the slump is determined. As a result of the compressive strength, the cast-down earth concrete is measured to be about 59% to 71% of the concrete strength. As the amount of PP fibers mixed in increased, the compression strength showed a relative decrease. As a result of tensile strength, it is found that the granite concrete is about 68-67% of concrete tensile strength. It was found that the compression strength decreased as the amount of PP fibers mixed in concrete or fire-gant concrete was increased. Then, when polypropylene fibers are mixed in the concrete and the concrete, it is found that tensile strength is increased. By analyzing these results, a fixed amount of PP fiber is mixed in the concrete mixed with the granite soil and utilized for various structures in the field of landscape construction or materials related to packaging, the prevention and improvement effect of the structure is determined.

• KOREAN JOURNAL OF PACKAGING SCIENCE & TECHNOLOGY
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• v.28 no.3
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• pp.171-174
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• 2022

Cosmetic packing materials tend to be difficult to recycle when discarded due to the cosmetic industry's pursuit of aesthetics, functionality, and high value-added design. Pump packaging, which is widely used for the good preservation and discharge of cosmetics contents, is difficult to be separated and recycled because of a metal spring, which is in charge of pump resilience. In this study, a polypropylene spring was developed to replace the existing metal spring to improve the recyclability of the pump packaging for cosmetics, and was uni-materialized by applying to the cosmetic packing materials with 0.2 ml of discharge amount. In addition, performance test was conducted to verify the equivalence with the existing metal spring pumps as grounds for the commercialization of metal-free uni material pump packaging. The decompression leak test showed no leakage and displayed 14.8~17.5 N of pressing strength, 2.3~8.8 % of deviation in dispensing volume, and 4 occasions of pumping for initial discharge.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.29 no.6D
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• pp.719-726
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• 2009

In this study, a series of push-off tests for lean concrete, aggregate, asphalt subbases mainly used in Korea were performed to investigate the friction characteristics between the slab and subbase layers. Use of separation membrane and wet condition of subbase were other parameters in the tests. Horizontal displacements of the slabs and friction coefficients were measured at 1st loading, stable condition (2nd and 3rd loadings), and wet condition (4th loading) by applying 40mm/hour horizontal loadings. Larger maximum friction coefficients were measured in order of the lean concrete, asphalt, aggregate, and subbases using the separation membrane at 1st loading, and in order of the asphalt, aggregate, lean concrete, and subbases using the separation membrane at stable and wet conditions. The friction coefficients of the aggregate and asphalt subbases which did not used the separation membrane decreased by the wet condition while the subbases using the separation membrane were not affected. Additional push-off tests for effects of slab thickness and temperature sensitivity of asphalt will be performed. And, effects of the friction characteristics between the slab and subbase layers on behavior and performance of concrete pavements will be investigated by structural analyses using the test results.

• Research in Plant Disease
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• v.23 no.4
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• pp.348-354
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• 2017

To investigate occurrence of root-knot nematode (RKN) in plastic house of central area of Korea, 132 soil samples were collected in cucumber, water melon, tomato, red pepper and strawberry fields from 2013 to 2015. Among 132 soil samples, 65 soil samples (49%) were infested with RKN and mean density of RKN was 178 second-stage juveniles per $100cm^3$ soil (min. 1 ~ max. 3,947). The frequency of RKN by regional was the highest in Chuncheon with 80%, followed by Cheonan (68%), Nonsan (36%), Buyeo (33%) and Yesan (30%). The frequency of RKN by crops was the highest in tomato with 83%, followed by cucumber (61%), strawberry (41%), red pepper (30%), watermelon (26%). To identify the species of RKN, fifteen populations were selected for representative populations. As a phylogenetic analysis of 15 populations, southern root-knot nematode (Meloidogyne incognita), peanut root-knot nematode (M. arenaria) and northern root-knot nematode (M. hapla) were identified with 47%, 20% and 33% ratio, respectively. In crops, M. incognita, M. arenaria and M. hapla were detected in tomato, M. incognita and M. arenaria were detected in cucumber and watermelon, and M. hapla was detected in strawberry and lettuce. Thus, there should be a continuous management to major species of each crops to prevent dispersal of RKN damages.