• Horticultural Science & Technology
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• v.32 no.3
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• pp.303-309
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• 2014

The purpose of this study is to suggest indicators and proper standards to forecast the sensory quality of 'Hongisul' grape. Further, this study determines the optimum fruit load of 'Hongisul' grape reflecting consumer preference. Among several quality attributes, identified through instrumental and sensory evaluation, soluble solid-acid ratio was the most useful indicator of consumer preference for 'Hongisul' grapes. The ideal soluble solid-acid ratio for marketable grapes was found to be higher than 86.3. Analysis on the effects of fruit load control on fruit quality revealed a negative correlation where the latter increased as the former decreased. This trend is common for all grape species. The field with a fruit load of $1,400kg{\cdot}10a^{-1}$ exhibited a soluble solid-acid ratio of less than 86.3, uneven coloring and slow softening texture at the mature stage. However, fruit quality proved excellent in the field with a fruit load of $1,200kg{\cdot}10a^{-1}$ or less. It is therefore proposed that fruit load should be controlled to $1,200kg{\cdot}10a^{-1}$ or less when growing 'Hongisul' grapes to produce high quality grapes. We believe that this result can be used as a standard in judging harvest time and evaluating fruit quality.

• Korean Journal of Agricultural and Forest Meteorology
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• v.13 no.2
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• pp.93-100
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• 2011

The study was conducted to investigate the correlation analysis between fruit quality and climatic parameters in grapevines. 'Campbell Early', one of the major grape cultivars, occupies more than 70% of cultivated areas in Korea. Recent research results have shown that the movement of cultivation area of fruit production resulted from the temperature increase. In this study, we investigated the relationship between fruit quality and climatic conditions in 13 major 'Campbell Early' grape producing areas (i.e., Sacheon, Jinju, Naju, Dangjin, Cheonan, Gimcheon, Yeongdong, Okcheon, Sangju, Suwon, Yeongju, Gangneung, Chuncheon, and Yanggu). The earliest and latest full blooming days of grapevines were ovserved on May 25 in Sacheon area and on June 7 in Yanggu area, respectively. At least 90 days are required for grapes to mature enough for the production of highquality fruits. The southern areas with less than 90 days of maturity period had difficulty in the production of good fruit. However, the areas with longer maturity period of 100 to 110 days produced grapes with with high sugar content and good coloring. The fruit qualities of 'Campbell Early' grapes are more closely related with temperature than other climatic factors. High temperature resulted in fruits with high acidity and delayed the coloration of the fruit skin in the southern area. The fruit skin was thin in the southern area and inclined to be thicker in the northern areas. Therefore, grape should be cultivated in regions with long maturity period to have high quality.

• Journal of Embryo Transfer
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• v.18 no.3
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• pp.237-242
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• 2003

These studies were carried out to establish an effective in vitro embryo transfer methods by analyzing several factors. The base media were TCM-199 solution for in vitro maturation(IVM) of bovine follicular oocytes and Fer-TALP solution for in vitro fertilization(IVF) and CRlaa medium for in vitro culture(IVC). IVC used the fertilized oocytes of 24-hr culture (day 1)after IVF. Embryos were cultured in drop-culture that contained 25 embryos per 10${mu}ell$. Blastocysts cultured for 7 to 9 in vitro were transferred to recipients. The results obtained were as follows: 1. The pregnancy rate according to different region of embryo transfer were 33.8％, 48.1％, 45.0％ and 35.3％ respectively. 2. The pregnancy rate according to the parity of recipient when embryos were transferred to nulliparous (42.9％) was higher than that of 1∼3nd parlous(36.9％), however there were not show significant difference each other. 3. According to the stage of blastocyst, the pregnancy rate when middle blastocysts (MB) (45.5％) were transferred to recipients were higher than that of late blastocysts (LB) (41.0％). 4. When IVF-derived blastocysts cultured for 7 to 9 day were transferred to recipients, the pregnancy rate was higher 7 day of blastocyst than that of 8 day or 9 day of blastocyst. The results of embryo transfer according to the regions, the parity of recipient and the development stage showed that blastocyst formed for 7day transferred to nulliparou were higher pregnancy rate than others.

• Korean Journal of Animal Reproduction
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• v.26 no.2
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• pp.173-182
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• 2002

The mechanisms underlying of the visual assessment and resulting in optimum embryonic development following in vitro maturation, fertilization, and culture are unclear, It was known that in vitro produced embryos show more frequent occurrence of fragmentation, which resulted in poor developmental potential and decreased implantation rate. The objective of this study was to investigate the apoptotic rates in bovine blastocyst derived from in vitro fertilization (IVF) and nuclear transfer (NT). In addition, the expression levels of Bcl-2 and Bax gene were investigated in the blastocyst to confirm their potential roles in the regulation of apoptosis during preimplantation embryonic development. Analysis of apoptosis was carried out by using terminal deoxynucleotidyl transferase mediate dUTP nick end labeling (TUNEL) method. The levels of Bcl-2 and Bax gene in the blastocyst derived from IVF and NT were determined by RT-PCR. The proportion of TUNEL positive signal in blastocyst derived from NT was significantly higher than that in blastocyst derived from IVF (p<0.001). Bcl-2 expression level of blastocyst derived from IVF was higher than that of blstocyst derived from NT. However, high expression level of Bax was observed in the blastocyst derived from NT. These results indicates that apoptosis is more responsible for fiagmentation in bovine blastocyst derived from NT than IVF. These results suggested that the increase of developmental failure followed by NT could be caused by nuclear fragmentation as apoptosis.

• KSBB Journal
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• v.11 no.4
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• pp.398-404
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• 1996

Escherichia coli was inoculated in calcium alginate capsules and cultivated to prepare encapsulated whole cell ${\beta}$-galactosidase. The dry cell weight in the capsule reached 100 g/L based on the inner space of the capsule. The activity of the encapsulated whole cell ${\beta}$-galactosidase increased with the dry cell weight increase during cultivation in the production medium. The activity of the encapsulated whole cell ${\beta}$-galactosidase was increased 25% by adding $2{\times}10^{-4}M Zn^{+2}$ ion in the production medium and 10% by coencapsulating with 2% (v/v) sunflower seed oil. The activity of encapsulated whole cell ${\beta}$-galactosidase produced in the concentric air lift reactor in which kLa was 82/hr was 86% higher than that in the shaking flask incubator where kLa was 2.55/hr.

• Proceedings of the Korean Society of Developmental Biology Conference
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• 2003.10a
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• pp.139-139
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• 2003

최근, 한우의 수정란 체외이식두수가 급격히 늘어나고 있으나, 모축의 등록번호를 대부분 확인 할 수 없으며, 확인이 가능할지라도 개체별 수정란의 생산이 거의 이루어지지 않고 있다. 본 실험에서는 모축의 등록번호가 확인된 개체의 난소로부터 채취한 난포란만을 체외성숙, 수정, 배양하여 배 발생율을 조사하고, 동결성적을 조사하였다. 도축된 한우의 난소를 개체별로 채취하여 2$0^{\circ}C$생리식염수에 넣어 3시간 이내에 실험실로 운반하고, 난포란을 채취하였다 개체별로 채취한 난포란은 9~47개/난소였고, 평균 14.3개/난소였다. 개체별 난포란은 IVMD 101 (일본, 펩티트연구소)에서 22물24시간 동안 체외 성숙시킨 후, IVF 100(일본, 펩티트연구소)으로 2회 세정한 후, 각각의 배양액 50${\mu}\ell$ 소적에 5개씩 5~6시간 수정시켰다. 수정란을 저 산소 배지 IVD 101 (일본, 펩티트연구소)로 7~9일간 배양하여 배 발생율을 조사하고 동결하였다. 평균 수정율은 81%, 배반포의 발생율은 35% 였으며, 동결 가능한 수정란은 평균 4개/두 정도 얻을 수 있었다. 이상의 결과로 한우의 개체가 확인된 난소로부터 수정란생산이 가능하고, 육질등급이 우수한 한우의 수정란생산이 가능해졌으며, 앞으로 고 능력 젖소의 난소에서도 고능력 수정란의 생산이 가능할 것으로 사료된다.

• Reproductive and Developmental Biology
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• v.28 no.1
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• pp.29-36
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• 2004

The objective of this study was to investigate the effects of amino acid supplementation of oocyte maturation medium on 1st polar body(PB) extrusion, embryo development and blastocsyt cell number. In experiment 1, Cumulus oocyte complexes(COCs) were matured in in vitro maturation(IVM) medium supplemented with 1, 2, or 4-fold of 10 $\mu$l/ml MEM non-essential amino acid(NEAA) and 20 Park, $\mu$ l/ml BME essential amino acid(EAA). The PB extrusion rate of oocytes matured in 1-fold amino acid group was significantly higher than that matured in medium without amino acid (p＜0.05), but it was decreased by the increase of the dosage of amino acid. There were no difference in the percentage of embryos reaching 2-cell, 8-cell and blastocyst in all treatments. The number of trophectoderm(TE) cells and total cell number of blastocysts were highest in 2-fold amino acid group, and the number of inner cell mass(ICM) cells was increased by the increase of the dosage of amino acid. In experiment 2, COCs were matured in IVM medium with 1, 5, or 10 mg/ml lactalbumin hydrolysate(LAH). The PB extrusion rate of oocytes matured in medium with 5 mg LAH was significantly higher than that matured in medium with 1 mg LAH (p＜0.05). The development rate to the blastocyst stage was significantly higher in non-supplement and 1 mg LAH group than in 5 mg and 10 mg LAH group (p＜0.05). The number of TE cells and total cell number did not differ among treatment groups, but the number of ICM cells was increased by the increase of LAH supplement. These results suggested that the supplement of certain group of amino acid in IVM medium effective on the quality of blastocyst, and further studies will be accompany with the search of new sources of amino acid used for the use of in vitro embryo production.

• Proceedings of the Korea Water Resources Association Conference
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• 2016.05a
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• pp.323-323
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• 2016

최근 강우강도 등의 기후변화로 인한 표토침식량이 증가하고 있고, 이에 따른 사회적 환경적 문제가 부각되고 있다. 특히 표토는 인류에게 식량생산의 기반이 되고, 정주공간을 제공할 뿐만 아니라 에너지 생산, 수자원 함양, 기후변화 대응, 생물 다양성 유지, 생태계의 건전성, 자원함양 및 순환, 오염물질 정화 등 소중한 생명자원이다. 이에 환경부에서는 2012월 12월 표토의 침식현황에 관한 고시를 제정하고, 표토 유실 대책 방안을 수립하기 위한 노력을 기울이고 있다. 이에 자원으로서의 표토를 보전하기 위한 단기적 관점의 대책 수립을 위한 웹GIS 기반 단일 강우에 의한 표토침식량을 예측하는 시스템을 개발하였다. 본 연구는 크게 표토침식 예측 모듈 개발과 정확성 평가를 위한 시험포 단위 모니터링 그리고 모듈을 적용한 웹GIS 시스템 개발, 시범적용을 위한 강원도 홍천군 자운리 DB 구축으로 구분된다. 표토침식예측 모듈의 정확성을 검증하기 위하여 가로 4m ${\times}$ 세로 22m, 경사도 3%, 9% 시험포 2개를 조성, 2015년 5월 11일부터 2015년 11월 23일까지 강우량, 유출량, 표토침식량을 조사하였으며, 웹 GIS 시스템은 Open Source Software인 Geoserver, PostGIS, OpenLayers를 활용하여 개발하였다. 마지막으로 개발된 웹GIS 표토침식예측시스템의 시범적용을 위하여 강원도 홍천군 자운리의 농경지 경계, 경사도, 경사장, 작물특성 등에 대한 GIS DB를 구축하였다. 시험포 모니터링 결과 강우발생일수는 총 64일로 관측되었고, 이중 유출은 총 30회가 발생되었다. 이 결과를 활용하여 표토침식 예측 모듈을 검증한 결과 3 % 시험포의 유출량 NSE : 0.88, $R^2$ : 0.91, 표토침식량 NSE : 0.87, $R^2$ : 0.90, 9 % 시험포의 유출량 NSE : 0.76, $R^2$ : 0.82, 표토 침식량 NSE : 0.82, $R^2$ : 0.88로 나타났다. 웹GIS 표토침식 예측 시스템은 Layer 정보, 맵, GIS tool, 경작기 정보, 날씨 정보 등으로 구성되어 있으며, 기상청 Open API와 연동하여 당일의 강수량 예보 데이터와 표토침식량 산정 모듈을 이용하여 예측 표토 침식량 데이터를 제공한다. 하루가 지나면 기상청에서 실측한 강수량 데이터를 이용하여 표토 침식량 산정모듈이 자동적으로 수행된 뒤 실측 강우량에 대한 표토침식량 정보가 제공된다 본 연구에서 개발된 웹GIS기반 표토침식 예측 시스템은 시범 대상 유역인 강원도 홍천군 자운리 유역을 대상으로 구축되었으며, 지속적으로 대상유역을 확대할 계획이다.

• Korean journal of applied entomology
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• v.60 no.1
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• pp.79-90
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• 2021

Landscape composition and structure are important factors determining biological diversity including pests and natural enemires in agricultural ecosystem. This study was conducted to indentify effect of landscape composition on occurrence of lepidopteran pest population in Geochang, Gyoungdnam. For this, orchard characteristics and management practices were surveyed in 80 conventional apple orchards in Geochang, Korea, along with the monitoring of pest population densities. The landscape features of each surveyed orchard also obtained by extracting information from the public-service map. Grapholita molesta was the most dominat and damaging pest followed by Phyllonorycter ringoniella and Carposina sasakii in trap catches. Adoxophyes paraorana occurrences were low. Farmers spray insecticides and fungicides ap. 12.4 times per year respectively while acaricides were sprayed 2.4 times. Major landscape features such as surrounding apple orchard or paddy field did not influence the pest populations but presence of plum, peach, wild peach, graph, and even abandoned orchards significantly resulted in higher pest population mostly on G. molesta. C. sasakii population was higher in orchards with grape, peach, and P. ringoniella with peach, grape, abandoned orchards and jujube. Results highlight the need of landscape management not only for the rural amenity but also for increasing functional diversity of agroecosystem as well as reducing pest population.

• Food Science and Preservation
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• v.5 no.4
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• pp.331-334
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• 1998

Weight loss of table Grape packed with control(Corrugated paperboard box), LDPE, CE(MA film masterbatched by ceramic powder treated cemical reagent) and FC box(laminated by CE film pouch) at 25 C after 10 days were 5.6%, 0.4%, 0.6%, and 0.7%, respectively. Weight loss of control was 7.29times higher than that of FC box. Total ascorbic acid content(TAA) of table grape after 10 days was 3.42 mg% for control, was 5.33 for LDPE and 5.14mg% for CE, 4.98mg% for FC. TAA of LDPE and CE showed to higher than that of control, especially TAA of FC was 33% higher than that of control. Titratable acid of LDPE and CE was high compare to control, and acidity of FC was 6% higher than that of control. References in overall appearance of LDPE, CE and FC were better than that of control. Corrugated paperboard box(FC) laminated with functional MA film showed to be able to used as packaging box of table grape.